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RNA pull-down联合质谱分析lncRNA HSFAS在增生性瘢痕中的作用及机制
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作者 夏童童 马芳 +8 位作者 孙浩原 刘虹麟 张正皓 杨佳琪 张慧萍 吴凯 沈江涌 姜怡邓 李桂忠 《中国组织工程研究》 CAS 北大核心 2025年第12期2492-2499,共8页
背景:课题组前期研究发现,增生性瘢痕特异性长链非编码RNA HSFAS是一种可用于增生性瘢痕诊断的新型生物标志物,但其如何在增生性瘢痕中发挥作用尚不清楚。目的:探讨长链非编码RNA HSFAS在增生性瘢痕中的作用及机制。方法:临床收集3例行... 背景:课题组前期研究发现,增生性瘢痕特异性长链非编码RNA HSFAS是一种可用于增生性瘢痕诊断的新型生物标志物,但其如何在增生性瘢痕中发挥作用尚不清楚。目的:探讨长链非编码RNA HSFAS在增生性瘢痕中的作用及机制。方法:临床收集3例行增生性瘢痕组织切除手术患者的新鲜增生性瘢痕皮肤组织和增生性瘢痕旁正常皮肤组织标本,应用免疫荧光技术检测两种皮肤组织冰冻切片中长链非编码RNA HSFAS的表达。应用酶消化法体外分离增生性瘢痕皮肤组织与正常皮肤组织原代成纤维细胞,采用qRT-PCR检测细胞中长链非编码RNA HSFAS mRNA表达,通过RNA pull-down联合质谱技术检测与长链非编码RNA HSFAS相互结合的蛋白,利用GO和KEGG分析长链非编码RNA HSFAS参与增生性瘢痕进展的主要功能和通路,通过catRAPID和RPISeq网站分析确定长链非编码RNA HSFAS与蛋白的靶向结合。结果与结论:①与正常皮肤组织相比,增生性瘢痕组织中的长链非编码RNA HSFAS表达升高(P<0.05);与正常皮肤组织来源成纤维细胞相比,增生性瘢痕组织来源成纤维细胞中长链非编码RNA HSFAS mRNA表达升高(P<0.05);②RNA pull-down联合质谱技术明确与长链非编码RNA HSFAS相互结合的蛋白有510个;GO和KEGG分析结果显示:这些蛋白主要涉及RNA剪接和加工、染色体合成和分离、细胞周期等过程,其中涉及RNA剪接和加工的蛋白有支架附着因子B2和DICER1,并且与长链非编码RNA HSFAS的结合分数较高;生物信息学技术分析验证结果显示,长链非编码RNA HSFAS与支架附着因子B2和DICER1蛋白存在相互结合;③结果显示,长链非编码RNA HSFAS可能通过与支架附着因子B2和DICER1蛋白相互结合调控RNA剪接和加工修饰影响基因表达,从而促进增生性瘢痕的发生发展。 展开更多
关键词 增生性瘢痕 lncRNA HSFAS 成纤维细胞 RNA pull-down 质谱分析
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Theoretical and experimental study of the pulling force of jet bits in radial drilling technology 被引量:5
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作者 Ruichang Guo Gensheng Li +3 位作者 Zhongwei Huang Shouceng Tian Xiaoning Zhang Wei Wu 《Petroleum Science》 SCIE CAS CSCD 2009年第4期395-399,共5页
Radial drilling technology, of which the jet bit is the key device, is a research focus in the field of oil drilling and production. This paper establishes mechanical equations for jet bits and analyzes the hydroseal ... Radial drilling technology, of which the jet bit is the key device, is a research focus in the field of oil drilling and production. This paper establishes mechanical equations for jet bits and analyzes the hydroseal of backward jets in bottom holes. Meanwhile this paper establishes a mechanical equation for a high pressure hose and analyzes the axial force distribution. Laboratory experiments indicate that the flow rate, the angle between the backward nozzle axis and the jet bit axis, and the hole diameter are the major influencing factors; the generation of the pulling force is mainly due to the inlet pressure of the jet bit; the backward jets can significantly increase not only the pulling force but also the stability of jet bits. The pulling force would reach 8,376 N under experimental conditions, which can steadily pull the high-pressure hose forward. 展开更多
关键词 Radial drilling water jet jet bit depression effect pulling force
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Graphene-tuned threshold gain to achieve optical pulling force on microparticle
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作者 Hong-Li Chen Yang Huang 《Chinese Physics B》 SCIE EI CAS CSCD 2021年第6期306-310,共5页
We investigate optical force on a graphene-coated gain microparticle by adopting the Maxwell's stress tensor method.It is found that there exists a threshold gain in obtaining the Fano-profile optical force which ... We investigate optical force on a graphene-coated gain microparticle by adopting the Maxwell's stress tensor method.It is found that there exists a threshold gain in obtaining the Fano-profile optical force which indicates the reversal of optical pushing and pulling force. And giant pushing/pulling force can be achieved if the gain value of the material is in the proximity of the threshold gain. Our results show that the threshold gain is more sensitive to the relaxation time than to the Fermi energy of the graphene. We further study the optical force on larger microparticle to demonstrate the pulling force occurring at octupole resonance with small gain value and then it will appear at quadrupole resonance by increasing gain value. Our work provides an in-depth insight into the interaction between light and gain material and gives the additional degree of freedom to optical manipulation of microparticle. 展开更多
关键词 pulling force threshold gain GRAPHENE MICROPARTICLE
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Pulling force of acoustic-vortex beams on centered elastic spheres based on the annular transducer model
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作者 Yuzhi Li Qingdong Wang +4 位作者 Gepu Guo Hongyan Chu Qingyu Ma Juan Tu Dong Zhang 《Chinese Physics B》 SCIE EI CAS CSCD 2020年第5期318-325,共8页
To solve the difficulty of generating an ideal Bessel beam,an simplified annular transducer model is proposed to study the axial acoustic radiation force(ARF)and the corresponding negative ARF(pulling force)exerted on... To solve the difficulty of generating an ideal Bessel beam,an simplified annular transducer model is proposed to study the axial acoustic radiation force(ARF)and the corresponding negative ARF(pulling force)exerted on centered elastic spheres for acoustic-vortex(AV)beams of arbitrary orders.Based on the theory of acoustic scattering,the axial distributions of the velocity potential and the ARF for AV beams of different orders generated by the annular transducers with different physical sizes are simulated.It is proved that the pulling force can be generated by AV beams of arbitrary orders with multiple axial regions.The pulling force is more likely to exert on the sphere with a smaller k0a(product of the wave number and the radius)for the AV beam with a bigger topological charge due to the strengthened off-axis acoustic scattering.The pulling force decreases with the increase of the axial distance for the sphere with a bigger k0a.More pulling force areas with wider axial regions can be formed by AV beams using a bigger-sized annular transducer.The theoretical results demonstrate the feasibility of generating the pulling force along the axes of AV beams using the experimentally applicable circular array of planar transducers,and suggest application potentials for multi-position stable object manipulations in biomedical engineering. 展开更多
关键词 acoustic radiation force pulling force acoustic-vortex beams annular transducer model acoustic scattering
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Optical pulling force on nanoparticle clusters with gain due to Fano-like resonance
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作者 马江南 冯侣 +3 位作者 王国富 林志方 郑红霞 陈华金 《Chinese Physics B》 SCIE EI CAS CSCD 2023年第1期334-339,共6页
We demonstrate that,in a simple linearly-polarized plane wave,the optical pulling forces on nanoparticle clusters with gain can be induced by the Fano-like resonance.The numerical results based on the full-wave calcul... We demonstrate that,in a simple linearly-polarized plane wave,the optical pulling forces on nanoparticle clusters with gain can be induced by the Fano-like resonance.The numerical results based on the full-wave calculation show that the optical pulling forces can be attributed to the recoil forces for the nanoparticle clusters composed of dipolar nanoparticles with three different configurations.Interestingly,the recoil forces giving rise to optical pulling forces are exactly dominated by the coupling term between the electric and magnetic dipoles excited in the nanoparticle clusters,while other higherorder terms have a negligible contribution.In addition,the optical pulling force can be tailored by modulating the Fano-like resonance via either the particle size or the gain magnitude,offering an alternative freedom degree for optical manipulations of particle clusters. 展开更多
关键词 optical pulling force nanoparticle clusters Fano-like resonance
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prAMD:一种GitHub pull-request协作机制自动设计方法
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作者 郑阳 郑丽伟 牟永敏 《计算机应用与软件》 北大核心 2024年第9期9-16,28,共9页
社会协作编码的开放性使得软件在开发过程中可以汇聚集体智慧。pull-request作为GitHub中开发人员贡献代码的主要方式,若开发人员之间不能有效地沟通协作,整个开发过程就会变得迟缓低效。因此为了提高协作效率,将pull开发模型下开发人... 社会协作编码的开放性使得软件在开发过程中可以汇聚集体智慧。pull-request作为GitHub中开发人员贡献代码的主要方式,若开发人员之间不能有效地沟通协作,整个开发过程就会变得迟缓低效。因此为了提高协作效率,将pull开发模型下开发人员之间的协作过程建模为一种Agent协作模型。结合实际开发过程中开发人员扮演的不同角色,以及各个角色具有的相关特征,基于自动机制设计方法计算得到满足优化目标的协作机制,一定程度上可以更好地促进开发人员协作,提高开发效率。 展开更多
关键词 GitHub pull-request 自动机制设计
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Up-pulling force analysis for ESC hollow cylindrical casting
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作者 An-guo Wang Zhou-hua Jiang +7 位作者 Yan-chun Lou Ji Tang Yun-long Xiong Yan-wu Dong Yan-wu Dong Fu-bin Liu Jing-chang Cheng Rui Chen 《China Foundry》 SCIE 2020年第1期48-55,共8页
Electroslag casting(ESC)is an important method to produce high quality castings.In this study,the ESC up-pulling inner mold method(EUPIM)was used to produce hollow cylindrical castings with the multiple consumable ele... Electroslag casting(ESC)is an important method to produce high quality castings.In this study,the ESC up-pulling inner mold method(EUPIM)was used to produce hollow cylindrical castings with the multiple consumable electrodes.The radial deformation,the axial and radial internal stress of the inner mold,and the axial internal stress of the slag shell were analyzed using the finite element method(FEM)with the aid of ANSYS software.The ProCAST software was used to calculate the specific heat,heat conductivity and density curve of Cu.Simulation results show that the radial deformation,the axial and radial internal stress of the inner mold,and the axial internal stress of the slag shell near the slag-metal interface of hollow cylndrical casting gradually increase from 0 s to 360 s after the ESC starting(slagging)process but before applying the up-pulling force.The suitable initial up-pulling moment of the inner mold is at around 180-198 s after the starting process. 展开更多
关键词 ESC up-pulling EUPIM hollow cylindrical casting FEM internal stress sustainability
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Pulling the turnip
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《小雪花(小学快乐作文)》 2004年第22期38-41,共4页
Hare:Oh,a turnip!A big turnip!I’m hungry.I’ll pull it out.Ai-Hay-Yo,Ai-Hay-Yo.The turnip is too heavy.I can’t pull it out.Dog:I’m a dog.I’m hungry.Oh,Miss Hare,What are you doing?
关键词 pulling the turnip
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Study on the Horizontal Movement State of Suction Anchor Piles for Offshore Wind Power during Horizontal Pulling 被引量:1
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作者 Jianping Jiang Jiaruo Gao 《World Journal of Engineering and Technology》 2022年第1期88-97,共10页
The subsea anchor piles of offshore wind power floating platform structures are mainly subjected to uplift and horizontal loads, and this paper focuses on the case of horizontal loads. A three-dimensional numerical si... The subsea anchor piles of offshore wind power floating platform structures are mainly subjected to uplift and horizontal loads, and this paper focuses on the case of horizontal loads. A three-dimensional numerical simulation study of the horizontal pullout characteristics of wind power suction anchor piles in clay layers was carried out to reveal the horizontal movement state of the anchor piles during horizontal pile pullout, the range of pile depth at the pullout point where the horizontal movement is achieved (referred to as the horizontal movement range), the relationship between the pullout load and the ultimate load during the horizontal movement, and the optimal location of the pullout point for the horizontal movement. The results show that at certain pull-out points, the anchor pile produces an overall horizontal movement state under suitable horizontal pull-out loads. The depth of the pile pull-out point for horizontal movement is in the middle and lower part of the pile, i.e. 14.2 m to 14.5 m. The horizontal pull-out load of 24,000 kN at a depth of 14.5 m within the pile horizontal movement range of 14.2m to 14.5 m is the maximum ultimate horizontal pull-out load;the optimum pull-out point depth is 14.5 m at 0.275 L (L is the pile length). For each pull-out point of the anchor pile in horizontal movement, the horizontal pull-out load in horizontal movement and the horizontal ultimate pull-out load existed and it was found that the two values were not exactly the same, the values were compared and it was found that at the optimum pull-out point the value of the ultimate horizontal pull-out load/horizontal pull-out load in horizontal movement tended to 1. 展开更多
关键词 Horizontal Movement State Suction Anchor Piles Horizontal Movement Range Optimum pull-Out Point Depth Offshore Wind Power
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THE CLINICAL ANALYSIS ON THERAPEUTIC EFFECT ON 47 CASES OF CERVICAL HEADACHE AND VERTIGO TREATED WITH ACUPUNCTURE,COMBINED WITH LIFTING,ROTATING,PULLING-MANIPULATION AT NECK
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作者 Zhang LuAcupuncture-Moxibustion and Tuina Department, Beijing University of TCM, Beijing 100029, ChinaTang JierenInstitute of Acupuncture and Moxibustion, China Academy of TCM, Beijing 100700, China 《World Journal of Acupuncture-Moxibustion》 1994年第4期12-14,共3页
The cervical headache and vertigo were treated with acupuncture at Fengchi(GB20)and Wangu(GB 12)and lifting,rotating,pulling-manipulation at neck.The total effective ratewas 91.4%.The therapeutic effects of these... The cervical headache and vertigo were treated with acupuncture at Fengchi(GB20)and Wangu(GB 12)and lifting,rotating,pulling-manipulation at neck.The total effective ratewas 91.4%.The therapeutic effects of these two types,malposition type and osteophytosis type,were studied and compared.The results were significantly different in statistics. 展开更多
关键词 Malposition Osteophytosis ACUPUNCTURE LIFTING ROTATING pulling-manip- ulation at NECK Cervical headache and VERTIGO
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Push/Pull和CONWIP/Pull生产控制模式对比分析 被引量:6
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作者 林勇 乐晓娟 付秋芳 《工业工程》 2006年第2期6-10,共5页
针对制造企业如何有效地进行生产控制的问题,对Push/Pull、CONWIP、CONWIP/Pull生产控制模式作了详细介绍,对比分析了Push/Pull、CONWIP/Pull两种混合生产控制,对混合生产控制方法做出了研究展望。
关键词 生产控制模式 Push/pull CONWIP/pull 对比分析
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GST Pull-down实验鉴定NF-κB相互作用多肽 被引量:5
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作者 李生茂 梁华平 +1 位作者 徐祥 刘东擘 《免疫学杂志》 CAS CSCD 北大核心 2006年第1期94-97,共4页
目的体外鉴定NF-κB相互作用多肽与p50和p65间的相互作用。方法首先构建GST-作用多肽融合蛋白的原核表达载体pET-42a/polypeptide及NF-κB p50和p65亚基保守结构域的原核表达载体pET22b/p50和pET22b/p65,并在大肠杆菌E.coliBL-21中诱导... 目的体外鉴定NF-κB相互作用多肽与p50和p65间的相互作用。方法首先构建GST-作用多肽融合蛋白的原核表达载体pET-42a/polypeptide及NF-κB p50和p65亚基保守结构域的原核表达载体pET22b/p50和pET22b/p65,并在大肠杆菌E.coliBL-21中诱导表达,后进行GST pull-down实验验证多肽与NF-κB p50和p65的结合效应。结果经诱导表达获得了可溶性的GST-多肽融合蛋白和具有DNA结合活性的p50p、65蛋白,GST pull-down实验证实3条多肽与p50发生特异地相互作用。结论证实3条多肽在体外能与p50发生物理性的相互作用,这为获得靶向NF-κB的功能拮抗多肽工作奠定了基础。 展开更多
关键词 GST pull—down实验 多肽 NF—κB p50/p65 蛋白间相互作用
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文献信息检索与PUSH、PULL技术 被引量:5
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作者 秦荪涛 潘元康 《图书馆杂志》 CSSCI 北大核心 2000年第6期30-32,36,共4页
文章探讨了在资料文献信息检索中使用PUSH和PULL技术的结合,提出一种新的信息检索模式理论,有利于提高科研工作效率,方便用户资料检索。
关键词 文献信息检索 PUSH pull 模式
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基于时间竞争的Push & Pull供应链运营机制 被引量:10
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作者 马士华 沈玲 《管理学报》 2005年第3期347-351,共5页
在对传统的供应链运营模式Push模式和Pull模式进行基本要素比较和流程比较的基础之上,提出了基于时间竞争的Push &Pull综合供应链运营机制,阐述了该模式的运作构架,以及Push &Pull结合点的确定和运作。
关键词 供应链运营机制 多阶响应周期 Push模式 pull模式
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ZCT Scheme of Push-Pull Forward Converter 被引量:4
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作者 张方华 王慧贞 严仰光 《Journal of Southeast University(English Edition)》 EI CAS 2002年第4期331-335,共5页
The paper proposes a novel zero current transition (ZCT) push pull forward converter. The auxiliary resonant cell is in parallel with the main circuit and the zero current switching (ZCS) range of the main and the aux... The paper proposes a novel zero current transition (ZCT) push pull forward converter. The auxiliary resonant cell is in parallel with the main circuit and the zero current switching (ZCS) range of the main and the auxiliary switches of the proposed converter are entirely achieved. The resonant capacitor achieves high voltage by operating the auxiliary cell. The auxiliary switch turns on before the main switches turn off, the high voltage of resonant capacitor blanks off the rectifier, and then the curr... 展开更多
关键词 converter/ZCT converter ZCS/ZCT push pull/push pull forward
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分销网络多级存储的RFID使能的Pull控制系统 被引量:3
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作者 李国家 汪定伟 《系统管理学报》 CSSCI 北大核心 2016年第3期571-576,共6页
射频识别(RFID)和物联网技术能够实现出入库产品快速自动扫描、精确计量以及订发货数量等物流信息在供应链上下游节点企业间的实时传递,为供应链存储控制提供了新方法。基于此,设计了RFID使能的远程电子看板系统,实现了供应链分销网络... 射频识别(RFID)和物联网技术能够实现出入库产品快速自动扫描、精确计量以及订发货数量等物流信息在供应链上下游节点企业间的实时传递,为供应链存储控制提供了新方法。基于此,设计了RFID使能的远程电子看板系统,实现了供应链分销网络多级存储的RFID使能的Pull控制,并对涉及的RFID使能的电子看板、分销网络多级存储的Pull控制以及网络数据库等关键技术进行了分析。最后,通过分别对RFID使能的Pull策略与(r,Q)策略下多种结构供应链分销网络多级存储的仿真,验证了RFID使能的Pull策略能够有效降低存储成本、提高服务水平。 展开更多
关键词 射频识别 供应链 多级存储 pull控制 仿真
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大粒度Pull Request描述自动生成 被引量:2
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作者 邝砾 施如意 +2 位作者 赵雷浩 张欢 高洪皓 《软件学报》 EI CSCD 北大核心 2021年第6期1597-1611,共15页
在GitHub平台中,许多项目贡献者在提交Pull Request(PR)时往往会忽略提交PR描述,这使得提交的PR容易被评审者忽略或者拒绝.因此,自动生成PR描述以帮助项目贡献者提高PR通过率是很有必要的.然而,现有PR描述生成方法的表现会受到PR粒度影... 在GitHub平台中,许多项目贡献者在提交Pull Request(PR)时往往会忽略提交PR描述,这使得提交的PR容易被评审者忽略或者拒绝.因此,自动生成PR描述以帮助项目贡献者提高PR通过率是很有必要的.然而,现有PR描述生成方法的表现会受到PR粒度影响,无法有效为大粒度的PR生成描述.因此,该工作专注于大粒度PR描述的自动生成.首先对PR中的文本信息进行预处理,将文本中的单词作为辅助节点构建词-句异质图,以建立PR语句间的联系;随后对异质图进行特征提取,并将提取后的特征输入至图神经网络进行图表示学习,通过节点间的消息传递,使句子节点学习到更丰富的内容信息;最后,选择带有关键信息的句子组成PR描述.此外,针对PR数据集缺少人工标注的真实标签而无法进行监督学习的问题,使用强化学习指导PR描述的生成,以最小化获得奖励的负期望为目标训练模型,该过程与标签无关,并且直接提升了生成结果的表现.在真实的数据集上进行了实验,实验结果表明,提出的大粒度PR描述生成方法在F1值和可读性上优于现有方法. 展开更多
关键词 pull Request描述 异质图神经网络 强化学习 非结构性文档 摘要生成
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扭臂式静电微驱动器的pulli-n现象分析 被引量:1
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作者 孙东明 董玮 +3 位作者 玄伟 刘彩霞 王国东 陈维友 《微纳电子技术》 CAS 2006年第5期251-253,共3页
从扭臂式微驱动器模型出发,分析了器件在静电驱动条件下pulli-n现象的产生条件,并给出公式化结果。讨论了器件的几何结构参数对于pulli-n现象的影响,并对具体结构的器件给出了pulli-n角度和pulli-n电压等方面的分析结果。对于特定的扭... 从扭臂式微驱动器模型出发,分析了器件在静电驱动条件下pulli-n现象的产生条件,并给出公式化结果。讨论了器件的几何结构参数对于pulli-n现象的影响,并对具体结构的器件给出了pulli-n角度和pulli-n电压等方面的分析结果。对于特定的扭臂结构,pulli-n角度为悬臂梁最大扭转角度的44.04%,且与扭臂的结构参数无关。 展开更多
关键词 微驱动器 静电驱动 扭臂 pull—in现象
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A NEW SOFT-SWITCHING PUSH-PULL THREE-LEVEL CONVERTER 被引量:4
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作者 马运东 阮新波 严仰光 《Transactions of Nanjing University of Aeronautics and Astronautics》 EI 2002年第2期127-133,共7页
The application areas of conventional push pull converters are limited because of high voltage stress of switches (twice of input voltage). This paper presents a novel zero voltage and zero current switching (ZCS)... The application areas of conventional push pull converters are limited because of high voltage stress of switches (twice of input voltage). This paper presents a novel zero voltage and zero current switching (ZCS) PWM push pull three level converter in which the voltage stress of switches is input voltage. With phase shifted modulation strategy, the leading switches can only realize zero voltage switching (ZVS), and the lagging switches can realize ZCS when block capacitor and block diodes are added. Using the strategy, the converter overcomes the drawbacks presented by the conventional push pull converter, such as magnetic aberration, large switch loss, and voltage spike on switches, so it can get higher efficiency, and a wider application area. The operating principle of the new converter is analyzed and verified on a 600 W, 50 kHz experimental prototype. Several zero voltage and zero current switching PWM push pull three level converters are proposed. 展开更多
关键词 zero voltage and zero current switching push pull converter three levels
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基于信息熵的Push/Pull策略下供应链不确定性 被引量:1
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作者 赵文丹 汪定伟 《东北大学学报(自然科学版)》 EI CAS CSCD 北大核心 2019年第4期457-460,466,共5页
以3级多产品供应链为例,分别采用Push/Pull/混合Push&Pull/改进Push 4种控制策略运行供应链,采用总熵比量化供应链的全局不确定性.将基于仿真优化(SBO)与遗传算法(GA)相结合,解决了计算量大与不确定因素多的难题.以控制规律中的增... 以3级多产品供应链为例,分别采用Push/Pull/混合Push&Pull/改进Push 4种控制策略运行供应链,采用总熵比量化供应链的全局不确定性.将基于仿真优化(SBO)与遗传算法(GA)相结合,解决了计算量大与不确定因素多的难题.以控制规律中的增益为决策变量,对不确定性进行优化,计算出最优决策变量下的客户满意度、超量库存、延迟交货和总成本等常用性能指标.仿真结果表明:供不应求时,采用混合Push&Pull策略可以降低总成本;供大于求时,采用改进Push策略能最大程度降低供应链的不确定性. 展开更多
关键词 供应链 不确定性 信息熵 pull/Push策略 仿真优化
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