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Effect of BN52021 on NFκ-Bp65 expression in pancreatic tissues of rats with severe acute pancreatitis 被引量:13
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作者 Shi-Hai Xia Dian-Chun Fang Chun-Xiu Hu Hui-Ying Bi Yin-Zhi Yang Yao Di 《World Journal of Gastroenterology》 SCIE CAS CSCD 2007年第6期882-888,共7页
AIM: To investigate dynamic changes and significance of expression of NF-κBp65 in pancreatic tissues of rats with severe acute pancreatitis (SAP), as well as BN52021 effects. METHODS: Wistar male rats were random... AIM: To investigate dynamic changes and significance of expression of NF-κBp65 in pancreatic tissues of rats with severe acute pancreatitis (SAP), as well as BN52021 effects. METHODS: Wistar male rats were randomly divided into negative control group (NC group, n = 60), SAP-model group (SAP group, n = 60), and BN52021-treated group (BN group, n = 60), and each of the above groups was respectively divided into 6 subgroups at different time points after operation (1 h, 2 h, 3 h, 6 h, 12 h, and 24 h) (n = 10). By RT-PCR and Western blot, NF-κBp65 mRNA and its protein expression in pancreatic tissues of rats were detected respectively. RESULTS: The expression of NF-κBp65 mRNA dynamically changed in both SAP groups and BN groups. The mRNA level was higher in SAP groups than NC groups at 2 h, 3 h, 12 h, and 24 h after operation (P 〈 0.05), higher in BN groups than NC groups at all time points (P 〈 0.05), and higher in BN groups than SAP group at 1 h (P 〈 0.05). The NF-κBp65 protein level was higher in SAP groups than NC groups at 1 h, 3 h, and 6 h (P 〈 0.01), and 2 h, 12 h, and 24 h (P 〈 0.05), higher in BN groups than NC groups at all time points (P 〈 0.05), and lower in BN groups than SAP groups at 1 h, 3 h, and 6 h (P 〈 0.05). CONCLUSION: The expression of NF-κBp65 in pancreatic tissues is dynamically changed and the changes play an important role in pathogenesis of SAR BN52021 exerts therapeutic effects through reducing the expression level of NF-κBp65 protein in the early stage of SAR 展开更多
关键词 BN52021 PANCREATITIS NF-ΚB signaltransduction
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Signal transduction pathways in liver and the influence of hepatitis C virus infection on their activities 被引量:3
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作者 Magdalena M Dabrowska Anatol Panasiuk Robert Flisiak 《World Journal of Gastroenterology》 SCIE CAS CSCD 2009年第18期2184-2189,共6页
In liver,the most intensively studied transmembrane and intracellular signal transduction pathways are the Janus kinase signal transduction pathway,the mitogen-activated protein kinases signal transduction pathway,the... In liver,the most intensively studied transmembrane and intracellular signal transduction pathways are the Janus kinase signal transduction pathway,the mitogen-activated protein kinases signal transduction pathway,the transforming growth factor β signal transduction pathway,the tumor necrosis factor α signal transduction pathway and the recently discovered sphingolipid signal transduction pathway.All of them are activated by many different cytokines and growth factors.They regulate specific cell mechanisms such as hepatocytes proliferation,growth,differentiation,adhesion,apoptosis,and synthesis and degradation of the extracellular matrix.The replication cycle of hepatitis C virus(HCV) is intracellular and requires signal transduction to the nucleus to regulate transcription of its genes.Moreover,HCV itself,by its structural and non-structural proteins,could influence the activity of the second signal messengers.Thus,the inhibition of the transmembrane and intracellular signal transduction pathways could be a new therapeutic target in chronic hepatitis C treatment. 展开更多
关键词 LIVER Hepatitis C virus infection signaltransduction pathway PROLIFERATION APOPTOSIS
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Novel matrine derivative MD-1 attenuates hepatic fibrosis by inhibiting EGFR activation of hepatic stellate cells 被引量:20
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作者 Yi Feng Hai-yan Ying +3 位作者 Ying Qu Xiao-bo Cai Ming-yi Xu Lun-gen Lu 《Protein & Cell》 SCIE CAS CSCD 2016年第9期662-672,共11页
Matrine (MT), the effective component of Sophora fla- vescens Air, has been shown to have anti-inflammation, immune-suppressive, anti-tumor, and anti-hepatic fibrosis activities. However, the pharmacological effects... Matrine (MT), the effective component of Sophora fla- vescens Air, has been shown to have anti-inflammation, immune-suppressive, anti-tumor, and anti-hepatic fibrosis activities. However, the pharmacological effects of MT still need to be strengthened due to its relatively low efficacy and short half-life. In the present study, we report a more effective thio derivative of MT, MD-1, and its inhibitory effects on the activation of hepatic stellate cells (HSCs) in both cell culture and animal models. Cytological experiments showed that MD-1 can inhibit the proliferation of HSC-T6 cells with a half-maximal inhibitory concentration (ICs0) of 62 pmollL. In addition, MD-1 more strongly inhibits the migration of HSC-T6 cells compared to MT and can more effectively induce G0/G1 arrest and apoptosis. Investigating the biological mechanisms underlying anti-hepatic fibrosis in the presence of MD-I, we found that MD-I can bind the epidermal growth factor receptor (EGFR) on the surface of HSC-T6 cells, which can further inhibit the phospho- rylaUon of EGFR and its downstream protein kinase B (Akt), resulting in decreased expression of cyclin D1 and eventual inhibition of the activation of HSC-T6 cells. Furthermore, in rats with dimethylnitrosamine (DMN)- induced hepatic fibrosis, MD-1 slowed the development and progression of hepatic fibrosis, protecting hepatic parenchymal cells and improving hepatic functions. Therefore, MD-1 is a potential drug for anti-hepatic fibrosis. 展开更多
关键词 matrine derivative hepatic stellate cellhepatic fibrosis epidermal growth factor receptor signaltransduction pathway
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Ethylene Controls Autophosphorylation of the Histidine Kinase Domain in Ethylene Receptor ETR1 被引量:8
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作者 Jan Voet-van-Vormizeele Georg Groth 《Molecular Plant》 SCIE CAS CSCD 北大核心 2008年第2期380-387,共8页
Perception of the phytohormone ethylene is accomplished by a small family of integral membrane receptors. In Arabidopsis, five ethylene receptor proteins are known, including ethylene resistant I (ETR1). The hydroph... Perception of the phytohormone ethylene is accomplished by a small family of integral membrane receptors. In Arabidopsis, five ethylene receptor proteins are known, including ethylene resistant I (ETR1). The hydrophobic aminoterminal domain of these receptors contains the ethylene-binding site while the carboxyl-terminal part consists of a histidine kinase domain and a response regulator domain, which are well known elements found in bacterial two-component signaling. The soluble membrane-extrinsic carboxyl-terminal part of the receptor, which is likely to play an important role in signal transduction, showed intrinsic kinase activity when expressed and purified on its own. However, a correlation between signal input and autokinase activity was not established in these studies, as receptors were missing the trans- membrane amino-terminal sensor domain. Thus, it is still unclear whether autophosphorylation occurs in response to perception of the ethylene signal. Here, we report on autophosphorylation studies of purified full-length ETR1. Autoki- nase activity of the purified receptor is controlled by ethylene or by ethylene agonists like the π-acceptor compound cyanide. In fact, both signal molecules were able to completely turn off the intrinsic kinase activity, Furthermore, the observed inhibition of autophosphorylation in ETR1 by both molecules could be prevented when the ethylene antagonist 1-methyl-cyclopropene (MOP) was applied. 展开更多
关键词 ethylene pathway phytohormone receptor membrane protein two-component system signaltransduction Arabidopsis thaliana
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Roles of Arabidopsis Patatin-Related Phospholipases A in Root Development Are Related to Auxin Responses and Phosphate Deficiency 被引量:7
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作者 Steffen Rietz Georgi Dermendjiev +6 位作者 Esther Oppermann Fikadu Getah Tafesse Yunus Effendi Andre Holk Jane E. Parker Markus Teige Gunther F.E. Scherer 《Molecular Plant》 SCIE CAS CSCD 2010年第3期524-538,共15页
Phospholipase A enzymes cleave phospho- and galactolipids to generate free fatty acids and lysolipids that function in animal and plant hormone signaling. Here, we describe three Arabidopsis patatin-related phospholip... Phospholipase A enzymes cleave phospho- and galactolipids to generate free fatty acids and lysolipids that function in animal and plant hormone signaling. Here, we describe three Arabidopsis patatin-related phospholipase A (pPLA) genes AtPLAIVA, AtPLAIVB, and AtPLAIVC and their corresponding proteins. Loss-of-function mutants reveal roles for these pPLAs in roots during normal development and under phosphate deprivation. AtPLAIVA is expressed strongly and exclusively in roots and AtplalVA-null mutants have reduced lateral root development, characteristic of an impaired auxin response. By contrast, AtPLAIVB is expressed weakly in roots, cotyledons, and leaves but is transcriptionally induced by auxin, although AtplalVB mutants develop normally. AtPLAIVC is expressed in the floral gynaecium and is induced by abscisic acid (ABA) or phosphate deficiency in roots. While an AtplalVC-1 loss-of-function mutant displays ABA respon- siveness, it exhibits an impaired response to phosphate deficiency during root development. Recombinant AtPLA proteins hydrolyze preferentially galactolipids and, less efficiently, phospholipids, although these enzymes are not localized in chloroplasts. We find that AtPLAIVA and AtPLAIVB are phosphorylated by calcium-dependent protein kinases in vitro and this enhances their activities on phosphatidylcholine but not on phosphatidylglycerol. Taken together, the data reveal novel functions of pPLAs in root development with individual roles at the interface between phosphate deficiency and auxin signaling. 展开更多
关键词 Abiotic/environmental stress hormonal regulation protein phosphorylation/dephosphorylation signaltransduction gene regulation Arabidopsis.
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Jinghua Weikang capsule protects against Helicobacter pylori-induced inflammatory responses via the nuclear factor-kappa B signaling pathway 被引量:4
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作者 Shi Zongming Ye Hui +3 位作者 Yu Jing Cheng Hong Li Jiang Zhang Xuezhi 《Journal of Traditional Chinese Medicine》 SCIE CAS CSCD 2018年第3期366-372,共7页
OBJECTIVE: To investigate the inhibitory effect of Jinghua Weikang capsule(JWC) on gastric inflammation induced by Helicobacter pylori(H. pylori)via the nuclear factor-kappa B(NF-κB) signaling pathway in Kunming mice... OBJECTIVE: To investigate the inhibitory effect of Jinghua Weikang capsule(JWC) on gastric inflammation induced by Helicobacter pylori(H. pylori)via the nuclear factor-kappa B(NF-κB) signaling pathway in Kunming mice.METHODS: We investigated the anti-inflammation potential of JWC extract in vivo in a H. pylori-induced gastritis mouse model. The expression of inflammation-related molecules was evaluated by Western blotting, and the concentrations of in vivo inflammatory markers were measured by enzyme-linked immunosorbent assay. Inflammatory cell infiltration was evaluated by histopathological examination, and m RNA levels of related genes were evaluated by quantitative reverse transcription polymerase chain reaction.RESULTS: JWC had a dose-dependent protective effect against H. pylori-induced gastritis by protecting gastric epithelial cells and inhibiting inflammatory cell infiltration. Mechanistically, JWC decreased the protein levels of phosphorylated IκBαand NF-κB p65, m RNA levels of NF-κB pathway molecules, and plasma levels of tumor necrosis factor-α and interleukin 1 beta.CONCLUSION: An important finding of our study is that JWC attenuated gastrointestinal inflammation and ulceration and exerted a protective effect against gastric injury via inhibition of inflammation reactions and regulating the canonical NF-κB signaling pathway in vivo. 展开更多
关键词 HELICOBACTER PYLORI NF-KAPPA B signaltransduction Jinghua Weikang CAPSULE
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