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A Brief Discussion on the Development of the Silk-Weaving Industry Along the “Southern Silk Road” in Yunnan 被引量:1
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作者 Lin Kaiqiang 《Contemporary Social Sciences》 2024年第1期18-33,共16页
Ancient Yunnan was one of the most significant regions along China’s ancient“Southern Silk Road.”During the Nanzhao period(738–902)of the late Tang Dynasty,Yunnan’s silk-weaving industry underwent a qualitative l... Ancient Yunnan was one of the most significant regions along China’s ancient“Southern Silk Road.”During the Nanzhao period(738–902)of the late Tang Dynasty,Yunnan’s silk-weaving industry underwent a qualitative leap as skilled silk craftsmen from the Bashu area migrated to Yunnan and introduced mulberry planting,silkworm breeding,and advanced silk-weaving techniques from Sichuan to the region.Consequently,people in Yunnan gradually acquired expertise in brocade weaving and embroidery.Many even mastered complex silk-weaving techniques.The development and progress of the silk-weaving industry in the ancient Yunnan region were intricately linked to the economic function and value of silk as both a commodity and currency along the“Southern Silk Road.”The local government in ancient Yunnan was greatly motivated by the economic interests brought by the development of silk-related industries and recognized the significance of developing the local silk industry.They even initiated a campaign to capture skilled silk craftsmen from Sichuan,aiming to foster the growth of the silk-weaving industry in Yunnan.After years of dedicated efforts from the local government in ancient Yunnan,the region emerged as a significant hub for silk production along China’s ancient“Southern Silk Road.”Despite the devastation caused by the wars in other parts of the country,Yunnan’s silk industry continued to thrive and provide ample silk products to sustain trade along this renowned route.In the contemporary era,amidst the decline of the silk-weaving industry in eastern China,Yunnan has proposed an industrial development strategy known as“relocating the silk-weaving industry from east to west.”This involves introducing advanced silk production techniques from the eastern regions into Yunnan to enhance and enrich its local silk industry,thereby establishing it as a traditional national sector and securing a competitive position within the global silk market.The historical experience of Yunnan’s silk industry demonstrated that economic development opportunities can only be seized through proactive endeavors rather than passive anticipation.The modern Yunnan silk industry,which upholds its historical traditions,continues to actively engage in international high-end technical cooperation,thus ensuring the enduring vitality of the ancient“Southern Silk Road.” 展开更多
关键词 Southern silk Road Bashu area YUNNAN silk-weaving technique
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The coordinated evolution of ecological environment,public service,and tourism economy along the Silk Road Economic Belt,using the Dual-Carbon Targets 被引量:1
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作者 Shuo Yang Wei Guo +1 位作者 Tianjun Xu Tongtong Liu 《Chinese Journal of Population,Resources and Environment》 2024年第1期34-47,共14页
Carbon peak and carbon neutrality(dual-carbon)are important targets for the international response to climate change.The Silk Road Economic Belt is a strategic resource region and is important for future ecological en... Carbon peak and carbon neutrality(dual-carbon)are important targets for the international response to climate change.The Silk Road Economic Belt is a strategic resource region and is important for future ecological environment and tourism development.Based on the“dual-carbon”targets,the Single index quantification,Multiple index synthesis,and Poly-criteria integration evaluation model were used in this study to measure the coordinated development index of the ecological environment,public service,and tourism economy along the Silk Road Economic Belt and to analyze its spatial and temporal evolution.Further,it explores the dynamic evolution and development trend of the three systems using the Kernel Density and Grey Markov Prediction Model.The results show that the coordinated development index along this region needs to be improved during the study period.Furthermore,the coordinated development index of the Southwest region is relatively higher than that of the Northwest region.From the development trend of the three systems,all of them develop in a stable manner;however,the tourism economy system is easily affected by external disturbances.The coordinated development index of the three systems changes dynamically and tends to be in a good state of coordination.There is a certain spatial and temporal heterogeneity.The gravity center of the coordinated development index has been in the Southwest region.During the forecast period,the coordinated development index along this region will improve significantly,while insufficient and unbalanced development will continue. 展开更多
关键词 Dual-carbon Ecological environment Public services Tourist economy silk Road Economic Belt
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Research on the Development of Fibroin and Nano-Fiber from Silk Cocoons for Regenerated Tissue Engineering Applications by Electro-Spinning
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作者 Md Kamrul Hasan Xinbo Ding 《Advances in Nanoparticles》 CAS 2024年第1期1-9,共9页
In this paper, the main goal is to prepare silk fibroin nano-fiber, which is used for regenerated tissue applications. Silk scaffold nano-fibers made by electro-spinning technology can be used in regenerated tissue ap... In this paper, the main goal is to prepare silk fibroin nano-fiber, which is used for regenerated tissue applications. Silk scaffold nano-fibers made by electro-spinning technology can be used in regenerated tissue applications. The purpose of the research is to prepare a silk-fibroin nano-fiber solution for potential applications in tissue engineering. Using a degumming process, pure silk fibroin protein is extracted from silk cocoons. The protein solution for fibroin is purified, and the protein content is determined. The precise chemical composition, exact temperature, time, voltage, distance, ratio, and humidity all have a huge impact on degumming, solubility, and electro-spinning nano-fibers. The SEM investigates the morphology of silk fibroin nano-fibres at different magnifications. It also reveals the surface condition, fiber orientation, and fiber thickness of the silk fibroin nano-fiber. The results show that regenerated silk fibroin and nano-fiber can be used in silk fibroin scaffolds for various tissue engineering applications. 展开更多
关键词 silk fibroin SCAFFOLD electro-spinning nano-fiber Tissue Engineering
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Piezoelectric fibers based on silk fibroin with excellent output performance
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作者 甄文强 陈杰 +1 位作者 范苏娜 张耀鹏 《Chinese Physics B》 SCIE EI CAS CSCD 2024年第8期128-135,共8页
The self-powered tissue engineering scaffold with good biocompatibility is of great significance for stimulating nerve cell growth.In this study,silk fibroin(SF)-based fibers with regulatable structure and piezoelectr... The self-powered tissue engineering scaffold with good biocompatibility is of great significance for stimulating nerve cell growth.In this study,silk fibroin(SF)-based fibers with regulatable structure and piezoelectric performance are fabricated by dry-spinning and post-treatment.The concentration of SF and calcium ion in spinning dope and the post-treatment affect the conformation transition and crystallinity of SF.As a result,the SF fibers exhibit high piezoelectric coefficient d_(33)(3.24 pm/V)and output voltage(~27 V).Furthermore,these piezoelectric fibers promote the growth of PC-12 cells,demonstrating the promising potential for nerve repair and other energy harvester. 展开更多
关键词 silk fibroin piezoelectric fiber DRY-SPINNING conformation transition
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A Review on Silk Fibroin as a Biomaterial in Tissue Engineering
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作者 Tkhu Chang Le Qian Zhang +3 位作者 Qingdi Qu Wentong Ding Sergej Anatolyevich Lazarev Shuang Pan 《Journal of Biosciences and Medicines》 2024年第3期275-290,共16页
Regenerative medicine progress is based on the development of cell and tissue bioengineering. One of the aims of tissue engineering is the development of scaffolds, which should substitute the functions of the replace... Regenerative medicine progress is based on the development of cell and tissue bioengineering. One of the aims of tissue engineering is the development of scaffolds, which should substitute the functions of the replaced organ after their implantation into the body. The tissue engineering material must meet a range of requirements, including biocompatibility, mechanical strength, and elasticity. Furthermore, the materials have to be attractive for cell growth: stimulate cell adhesion, migration, proliferation and differentiation. One of the natural biomaterials is silk and its component (silk fibroin). An increasing number of scientists in the world are studying silk and silk fibroin. The purpose of this review article is to provide information about the properties of natural silk (silk fibroin), as well as its manufacture and clinical application of each configuration of silk fibroin in medicine. Materials and research methods. Actual publications of foreign authors on resources PubMed, Medline, E-library have been analyzed. The selection criteria were materials containing information about the structure and components of silk, methods of its production in nature. This article placed strong emphasis on silk fibroin, the ways of artificial modification of it for use in various sphere of medicine. 展开更多
关键词 Tissue Engineering Biomaterial SCAFFOLD silk Fibroin
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Structural analysis of silk using solid-state NMR
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作者 Tetsuo Asakura 《Magnetic Resonance Letters》 2024年第3期19-34,共16页
Silkworms and spiders are capable of generating fibers that are both highly durable and elastic in a short span of time,using a silk solution stored within their bodies at room temperature and normal atmospheric press... Silkworms and spiders are capable of generating fibers that are both highly durable and elastic in a short span of time,using a silk solution stored within their bodies at room temperature and normal atmospheric pressure.The dragline silk fiber,which is essentially a spider's lifeline,surpasses the strength of a steel wire of equivalent thickness.Regrettably,humans have yet to replicate this process to produce fibers with similar high strength and elasticity in an eco-friendly manner.Therefore,it is of utmost importance to thoroughly comprehend the extraordinary structure and fibrillation mechanism of silk,and leverage this understanding in the manufacturing of high-strength,high-elasticity fibers.This review will delve into the recent progress in comprehending the structure of silks derived from silkworms and spiders,emphasizing the distinctive attributes of solidstate NMR. 展开更多
关键词 silk Bombyx mori SPIDER Solid-state NMR STRUCTURE
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Three-dimensional thermohaline structure estimation derived from HY-2 satellite data over the Maritime Silk Road and its applications
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作者 Zhiqiang Chen Xidong Wang +4 位作者 Xiangyu Wu Yuan Cao Zikang He Dakui Wang Jian Chen 《Acta Oceanologica Sinica》 SCIE CAS CSCD 2024年第5期41-53,共13页
Estimated ocean subsurface fields derived from satellite observations provide potential data sources for operational marine environmental monitoring and prediction systems.This study employs a statistic regression rec... Estimated ocean subsurface fields derived from satellite observations provide potential data sources for operational marine environmental monitoring and prediction systems.This study employs a statistic regression reconstruction method,in combination with domestic autonomous sea surface height and sea surface temperature observations from the Haiyang-2(HY-2)satellite fusion data,to establish an operational quasi-realtime three-dimensional(3D)temperature and salinity products over the Maritime Silk Road.These products feature a daily temporal resolution and a spatial resolution of 0.25°×0.25°and exhibit stability and continuity.We have demonstrated the accuracy of the reconstructed thermohaline fields in capturing the 3D thermohaline variations through comprehensive statistical evaluations,after comparing them against Argo observations and ocean analysis data from 2022.The results illustrate that the reconstructed fields effectively represent seasonal variations in oceanic subsurface structures,along with structural changes resulting from mesoscale processes,and the upper ocean’s responses to tropical cyclones.Furthermore,the incorporation of HY-2 satellite observations notably enhances the accuracy of temperature and salinity reconstructions in the Northwest Pacific Ocean and marginally improves salinity reconstruction accuracy in the North Indian Ocean when compared to the World Ocean Atlas 2018 monthly climatology thermohaline fields.As a result,the reconstructed product holds promise for providing quasi-real-time 3D temperature and salinity field information to facilitate fast decisionmaking during emergencies,and also offers foundational thermohaline fields for operational ocean reanalysis and forecasting systems.These contributions enhance the safety and stability of ocean subsurface activities and navigation. 展开更多
关键词 HY-2 satellite observations subsurface structures reconstruction Maritime silk Road operational thermohaline product
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Matching Dyeing and Properties of Silk Fabrics with Natural Edible Pigments
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作者 CHEN Yangyi ZHOU Shihang +4 位作者 SU Tong LI Jingzhi CHEN Hongshan QI Huan QIU Yiping 《Journal of Donghua University(English Edition)》 CAS 2024年第4期428-435,共8页
The silk fabrics were matching dyed with three natural edible pigments(red rice red,ginger yellow and gardenia blue).By investigating the dyeing rates and lifting properties of these pigments,it was observed that thei... The silk fabrics were matching dyed with three natural edible pigments(red rice red,ginger yellow and gardenia blue).By investigating the dyeing rates and lifting properties of these pigments,it was observed that their compatibilities were excellent in the dyeing process:dye dosage 2.5%(omf),mordant alum dosage 2.0%(omf),dyeing temperature 80℃and dyeing time 40 min.The silk fabrics dyed with secondary colors exhibited vibrant and vivid color owing to the remarkable lightness and chroma of ginger yellow.However,gardenia blue exhibited multiple absorption peaks in the visible light range,resulting in significantly lower lightness and chroma for the silk fabrics dyed with tertiary colors,thus making it suitable only for matte-colored fabrics with low chroma levels.In addition,the silk fabrics dyed with these three pigments had a color fastness that exceeded grade 3 in resistance to perspiration,soap washing and light exposure,indicating acceptable wearing properties.The dyeing process described in this research exhibited a wide range of potential applications in matching dyeing of protein-based textiles with natural colorants. 展开更多
关键词 matching dyeing silk fabric natural edible pigment secondary color tertiary color
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Preparation of Regenerated Silk Fibroin Hybrid Fibers with Hydrogen Peroxide Sensing Properties by Wet Spinning
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作者 Song Lu Jianjun Guo +3 位作者 Richard Ansah Herman Xinyi Wu Lin Ma Guohua Wu 《Journal of Renewable Materials》 EI CAS 2024年第6期1043-1055,共13页
Silk is widely used in the production of high-quality textiles.At the same time,the amount of silk textiles no longer in use and discarded is increasing,resulting in significant waste and pollution.This issue is of gr... Silk is widely used in the production of high-quality textiles.At the same time,the amount of silk textiles no longer in use and discarded is increasing,resulting in significant waste and pollution.This issue is of great concern in many countries where silk is used.Hydrogen peroxide as a naturally occurring compound is an important indicator of detection in both biology and the environment.This study aims to develop a composite fiber with hydrogen peroxide-sensing properties using discarded silk materials.To achieve this goal,firstly,polydopamine(PDA)was used to encapsulate the ZnFe_(2)O_(4) NPs to achieve the improvement of dispersion,and then regenerated silk fibroin(RSF)and PDA@ZnFe_(2)O_(4)/RSF hybrid fibers are prepared by wet spinning.Research has shown that PDA@ZnFe_(2)O_(4)/RSF demonstrates exceptional sensitivity,selectivity,and stability in detecting hydrogen peroxide,while maintaining high mechanical strength.Furthermore,the complete hybridization of PDA@ZnFe_(2)O_(4) with silk fibroin not only results in the combination of the durability of silk fibroin and PDA@ZnFe_(2)O_(4)’s rigidity,ensuring a reliable service life,but also makes PDA@ZnFe_(2)O_(4)/RSF exhibit excellent catalytic activity and biocompatibility.Therefore,the composite fiber exhibits exceptional mechanical properties and reliable hydrogen peroxide sensing capabilities,making it a promising material for biological and medical applications. 展开更多
关键词 Regenerated silk fibroin modified zinc ferrite nanoparticles composite fiber hydrogen peroxide sensor wet spinning
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Jili Silk
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《China Today》 2024年第4期80-80,共1页
JILI silk is a type of silk produced in Jili Village,Nanxun Town,Nanxun District,Huzhou City in east China’s Zhejiang Province.Jili Village has been producing silk since the village was established in the late Yuan D... JILI silk is a type of silk produced in Jili Village,Nanxun Town,Nanxun District,Huzhou City in east China’s Zhejiang Province.Jili Village has been producing silk since the village was established in the late Yuan Dynasty(1271-1368).Jili silk is characterized by being stretchy,soft and fine with an even thickness and shiny white color.With these features,Jili silk is widely recognized in China and across the world. 展开更多
关键词 Province. VILLAGE silk
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Synergistic effects of three traditional herbs green tea,mulberry leaf and corn silk on glucose uptake level of L6 myoblasts and the hypoglycemic mechanism
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作者 Jing-Na Zhou Ming-Yue Li +4 位作者 Ting-Ting Zhang Jing-Yang Lu Min Zhang Peng-Wei Zhuang Hai-Xia Chen 《Traditional Medicine Research》 2024年第7期1-12,共12页
Background:Green tea,mulberry leaf and corn silk are traditional herbs used in the prevention and treatment of diabetes in China for a long time,but their synergistic hypoglycemic effects and mechanisms remain unclear... Background:Green tea,mulberry leaf and corn silk are traditional herbs used in the prevention and treatment of diabetes in China for a long time,but their synergistic hypoglycemic effects and mechanisms remain unclear.Methods:The effective components of green tea,mulberry leaf and corn silk were extracted and enriched.Mixture design of experiments was used to study the influences of different combinations on the cell viability and glucose uptake level of L6 myoblasts,so as to determine the optimal synergistic hypoglycemic combination.The possible hypoglycemic mechanism of the optimal synergistic combination was explored by cytotoxicity assay,glucose uptake assay,and western blot.Results:Three polyphenol enrichment fractions of the herbs,30%ethanol elution fraction of green tea(GT),50%ethanol elution fraction of mulberry leaf(ML)and 60%ethanol elution fraction of corn silk(CS)were obtained.The antioxidant activities of GT-30%,ML-50%and CS-60%were superior to those of crude extracts,and showed strong potential inα-amylase andα-glucosidase inhibition activities.The optimal synergistic combination of crude extracts G7(crude extract of green tea:crude extract of mulberry leaf:crude extract of corn silk=1:5:3),polyphenol enrichment fractions R3(GT-30%:ML-50%:CS-60%=1:7:1)and monomers X2(epigallocatechin gallate:morusin:formononetin=3:1:2)were selected,respectively.G7,R3,and X2 showed promoting effects on the cell viability and glucose uptake of L6 myoblasts within the detected concentration range.In addition,G7,R3,and X2 could increase the expression levels of p-PI3K/PI3K and p-Akt/Akt in L6 myoblasts,and promote the translocation of Glut4,but G7 and R3 showed more significant effects.Conclusion:The synergistic hypoglycemic effects of green tea,mulberry leaf and corn silk had the characteristics of multiple-components and multiple-targets with p-PI3K/PI3K,p-Akt/Akt and the translocation of Glut4 signal pathways involved.The three traditional herbs might have the potential to be combined used for the prevention and treatment of diabetes based on the synergistic hypoglycemic effects. 展开更多
关键词 green tea mulberry leaf corn silk synergistic effect type 2 diabetes mellitus mechanism
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Study on the Protection of Sites of“Maritime Silk Road”in Haikou from the Perspective of World Cultural Heritage
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作者 CHEN Xu 《Journal of Landscape Research》 2024年第1期70-76,共7页
The sites of Maritime Silk Road in Haikou City are composed of sites of navigation and trade,religious temples and stone monuments,urban construction and coastal defense scattered on the south bank of the Qiongzhou St... The sites of Maritime Silk Road in Haikou City are composed of sites of navigation and trade,religious temples and stone monuments,urban construction and coastal defense scattered on the south bank of the Qiongzhou Strait.They played a key role in the formation and shaping of the settlements in Haikou City,recorded the process of intercommunication and integration between Chinese civilization and other regional cultures,and witnessed the germination,flourishing and inheritance of Haikou’s unique marine culture.The mixture of points,lines and planes blends with the urban area and coastline of Haikou City in spatial distribution.In this paper,from the perspective of world cultural heritage,some suggestions for the protection planning of sites of Maritime Silk Road in Haikou City were proposed based on the analysis of historical and geographical background,comparison of domestic and foreign similar sites,and evaluation of cultural heritage value. 展开更多
关键词 Maritime silk Road World cultural heritage Heritage value Protection planning
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Preparation and release of curcumin/silk fibroin/sodium alginate film
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作者 Yerong Yuan Jun’an Zheng +3 位作者 Zunchao Liu Wei Li Jiaqing Cao Xiangrong Zhang 《Journal of Polyphenols》 2024年第1期1-10,共10页
The aim of this study was to prepare silk fibroin/sodium alginate composite film containing curcumin by casting method.Orthogonal test was used to optimize the formulation according to the values of tensile strength a... The aim of this study was to prepare silk fibroin/sodium alginate composite film containing curcumin by casting method.Orthogonal test was used to optimize the formulation according to the values of tensile strength and elongation at break.The release of curcumin in the optimal film was studied in order to explore its application as wound dressing.The results showed that the optimum composition of curcumin/silk fibroin/sodium alginate composite film was as follows:Silk fibroin(70 mg/mL)2.7 g,sodium alginate(24 mg/mL)0.84 g,span 40(5.0 mg/mL)0.4 g,glycerol(3.75%,V/V)3 mL,curcumin(0.2 mg/mL)0.016 g.The optimum film showed the tensile strength and the elongation at break was(0.628±0.032)MPa and(0.794±0.046)%,respectively. 展开更多
关键词 CURCUMIN silk fibroin sodium alginate composite film
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Application of Traditional Chinese Culture in Cross-Cultural English Education:A Case Study of the Maritime Silk Road in Guangdong Province
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作者 Xinyu He 《Journal of Contemporary Educational Research》 2024年第10期16-22,共7页
The phenomenon of aphasia in Chinese culture is serious.The existing English teaching materials emphasize too much Western culture education and lack traditional Chinese cultural elements.Therefore,this paper takes th... The phenomenon of aphasia in Chinese culture is serious.The existing English teaching materials emphasize too much Western culture education and lack traditional Chinese cultural elements.Therefore,this paper takes the Guangdong Maritime Silk Road as an example to study the specific application of traditional Chinese culture in cross-cultural English education.This paper first summarizes the significance of cross-cultural integration into college English education and then points out the serious phenomenon of Chinese cultural aphasia.Next,the paper focuses on English education,using English textbooks as a starting point to explore and integrate strategies related to excellent traditional Chinese culture from the Guangdong Maritime Silk Road.By integrating traditional Chinese culture into business English classes(with the Guangdong Maritime Silk Road as an example),the study explores the influence of such cultural integration on students’cross-cultural communication skills,cultural identity,and learning effects.The results showed that the P value of the experimental group and the control group was<0.05,that is,cultural integration had a positive effect on improving the effect of cross-cultural English education.The overall scores and cultural confidence of the experimental group are higher than those of the control group,which proves that cross-cultural teaching has a positive effect on the improvement of students’scores. 展开更多
关键词 Cross-cultural English education Chinese traditional culture Guangdong Maritime silk Road Cultural confidence English learning effect
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Silk血流导向支架联合弹簧圈治疗颅内复杂动脉瘤的短期疗效 被引量:4
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作者 毛之奇 张鹏 +1 位作者 叶明 凌锋 《中国脑血管病杂志》 CAS 2013年第10期518-522,共5页
目的 探讨Silk血流导向支架治疗颅内复杂动脉瘤的初步疗效.方法 回顾性分析首都医科大学宣武医院2010年4月1日-2011年10月30日应用Silk支架治疗30例颅内动脉瘤患者的临床资料,其中男11例、女19例,年龄为29~71岁,平均(51.7±11.7)... 目的 探讨Silk血流导向支架治疗颅内复杂动脉瘤的初步疗效.方法 回顾性分析首都医科大学宣武医院2010年4月1日-2011年10月30日应用Silk支架治疗30例颅内动脉瘤患者的临床资料,其中男11例、女19例,年龄为29~71岁,平均(51.7±11.7)岁.30例动脉瘤患者有31个动脉瘤,30个动脉瘤位于颈内动脉,除1例患者床突上段有两个动脉瘤为小动脉瘤(分别为3 mm×4 mm和2 mm×2 mm)外,其余均为大型或巨大型动脉瘤(最大径 〉15 mm),左椎动脉颅内段基底动脉夹层动脉瘤1例.结果 ①30例患者中3例置入2枚Silk,1例置入3枚Silk,其余患者置入1枚Silk.1例(3.3%)支架未能到位,治疗失败,1例(3.3%)第3天出现蛛网膜下腔出血,停抗血小板聚集药物后3个月复查DSA,显示同侧颈内动脉闭塞,1例治疗后第19天消化道出血,停用抗凝药物后出现脑梗死.②17例(56.7%)患者获得了6个月的DSA随访,动脉瘤完全或几乎消失2例(11.8%),动脉瘤及颈内动脉同时闭塞的患者3例(17.6%),动脉瘤部分显影的患者12例(70.6%).结论 对使用常规弹簧圈难以致密栓塞的大型动脉瘤、宽颈动脉瘤或不规则形状的动脉瘤,Silk支架提供了一种治疗方法,但短期内动脉瘤闭塞率较低,其远期效果尚有待于进一步的随访. 展开更多
关键词 颅内动脉瘤 血管内操作 silk血流导向支架
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Screening and Cloning of RAPD Marker of Fluoride Tolerance Gene in Silkworm,Bombyx mori 被引量:4
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作者 徐庆刚 陈克平 +1 位作者 姚勤 刘晓勇 《Zoological Research》 CAS CSCD 北大核心 2004年第1期69-72,共4页
In this study,silkworm strain T6,tolerant to fluoride,and silkworm strain 733xin,highly sensitive to fluoride,were used to construct the near-isogenic lines.300 random primers were used in RAPD amplification to DNAs o... In this study,silkworm strain T6,tolerant to fluoride,and silkworm strain 733xin,highly sensitive to fluoride,were used to construct the near-isogenic lines.300 random primers were used in RAPD amplification to DNAs of these lines.A molecular marker named S207 was found linked to the fluoride tolerance gene.Examination to F 2 segregated individuals of the above lines verified that this molecular marker was reliable.Subsequently,the molecular marker was cloned into a T vector (pUCm-T) for sequencing.Comparing with sequences available in the GenBank showed that this molecular marker was novel.We plan to convert it into a SCAR marker to facilitate establishment of a molecular marker assisted breeding system. 展开更多
关键词 Bombyx mori Near-isogenic lines Fluoride tolerance RAPD marker
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Synthesis of Biomorphic ZrO2-CeO2 Nanostructures by Silkworm Silk Template 被引量:1
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作者 张宗见 李嘉 +2 位作者 孙富升 吴恒亮 邝丰栾 《Chinese Journal of Chemical Physics》 SCIE CAS CSCD 2010年第3期351-354,I0002,共5页
A simple and green technique has been developed to prepare hierarchical biomorphic ZrO2- CeO2, using silkworm silk as the template. Different from traditional immersion technics, the whole synthesis process depends mo... A simple and green technique has been developed to prepare hierarchical biomorphic ZrO2- CeO2, using silkworm silk as the template. Different from traditional immersion technics, the whole synthesis process depends more on the restriction or direction functions of the silkworm silk template. The analytic results showed that ZrO2-CeO2 exhibited a well-crystallized hierarchically interwoven hollow fiber structure with 16-28 μm in diameter. The grain size of the sample calcined at 800 ℃ was about 14 nm. Consequently, the interwoven meshwork at three dimensions is formed due to the direction of biotemplate. The action mechanism is summarily discussed here. It may bring the biomorphic ZrO2-CeO2 nanomaterials with hierarchical interwoven structures to more applications, such as catalysts. 展开更多
关键词 NANOMATERIAL CATALYST ZrO2-CeO2 Biomorphic silkworm silk template
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面向图书馆关联数据的语义链接构建研究——以Silk工具为例 被引量:2
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作者 游毅 《图书馆论坛》 CSSCI 北大核心 2014年第10期24-30,共7页
图书馆关联数据的链接类型包括概念间词汇型链接与实体间关系型链接,均适用基于属性相似度匹配的构建方法。基于此,图书馆能够依托Silk的链接定义语言与实现机制满足馆藏数据集的链接构建需求,包括利用数据存取参数设置获取目标链接数... 图书馆关联数据的链接类型包括概念间词汇型链接与实体间关系型链接,均适用基于属性相似度匹配的构建方法。基于此,图书馆能够依托Silk的链接定义语言与实现机制满足馆藏数据集的链接构建需求,包括利用数据存取参数设置获取目标链接数据对象,借助链接构建条件定义语义关联发现依据的相似度算法,利用链接路径选择指定相似度匹配的馆藏属性,借助语义关联预匹配策略提高构建效率,从而实现面向海量数据的大规模语义链接构建。 展开更多
关键词 关联数据 语义链接 链接构建 silk工具
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PVA-Silk复合水凝胶的启动摩擦性能研究 被引量:2
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作者 张德坤 程慧茹 +1 位作者 沈艳秋 葛世荣 《中国矿业大学学报》 EI CAS CSCD 北大核心 2008年第6期819-823,共5页
选用高分子聚乙烯醇(PVA)和脱胶后的蚕丝(Silk)为原料,采用反复冷冻-融化法制备PVA-Silk复合水凝胶.在光学显微镜上观察PVA-Silk复合水凝胶的微观形貌,在UMT-Ⅱ型微摩擦试验机上对PVA-Silk复合水凝胶开展往复式摩擦实验,研究接触时间、... 选用高分子聚乙烯醇(PVA)和脱胶后的蚕丝(Silk)为原料,采用反复冷冻-融化法制备PVA-Silk复合水凝胶.在光学显微镜上观察PVA-Silk复合水凝胶的微观形貌,在UMT-Ⅱ型微摩擦试验机上对PVA-Silk复合水凝胶开展往复式摩擦实验,研究接触时间、滑动速率及冷冻-融化次数对复合水凝胶的启动摩擦性能的影响.结果表明,随反复冷冻-融化次数的增加,PVA-Silk复合水凝胶的交联度增大,结晶度提高,其弹性模量增大,最大法向压缩变形量减小,且其基本恢复变形所需的时间减小;PVA-Silk复合水凝胶在滑动过程中的法向位移量和最大摩擦系数随接触时间的延长而增大,随反复冷冻-融化次数的增加而减少;但最大摩擦系数与滑动速率无明显的相关联性. 展开更多
关键词 聚乙烯醇 蚕丝 复合水凝胶 启动摩擦性能
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不同静电纺丝膜上骨髓间充质干细胞的黏附、增殖与成血管平滑肌分化 被引量:2
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作者 孙现娟 王秋花 +3 位作者 张锦艺 杨杨杨 王文双 张晓晴 《中国组织工程研究》 CAS 北大核心 2025年第4期661-669,共9页
背景:临床上迫切需要小口径人工血管来治疗冠状动脉和外周动脉疾病,目前,血管组织工程已成为制备小口径人工血管的主要方法,选择合适的生物材料和细胞来源是小口径组织工程血管构建成功的关键因素。目的:观察4种静电纺丝膜材料对骨髓间... 背景:临床上迫切需要小口径人工血管来治疗冠状动脉和外周动脉疾病,目前,血管组织工程已成为制备小口径人工血管的主要方法,选择合适的生物材料和细胞来源是小口径组织工程血管构建成功的关键因素。目的:观察4种静电纺丝膜材料对骨髓间充质干细胞增殖、黏附及分化为血管平滑肌细胞的影响。方法:分离提取SD大鼠骨髓间充质干细胞。将骨髓间充质干细胞分别接种于聚己内酯(PCL)、聚己内酯-透明质酸(PCL-HA)、聚己内酯-丝素蛋白(PCL-SF)、聚己内酯-明胶(PCL-GEL)静电纺丝膜材料上,培养1,3,7 d后,扫描电镜下观察材料上的细胞排布,鬼笔环肽染色观察材料上的细胞增殖与黏附,qRT-PCR检测材料上细胞分泌的CD90、Meflin、转化生长因子βmRNA表达;向血管平滑肌细胞诱导分化7 d后,qRT-PCR检测材料上细胞ɑ-平滑肌肌动蛋白mRNA表达。结果与结论:①扫描电镜下可见骨髓间充质干细胞在4种静电纺丝膜上均沿着静电纺丝膜的纤维走向排列;②鬼笔环肽染色显示,骨髓间充质干细胞在4种静电纺丝膜上分布规律,均沿着纤维走向呈现平行分布,并且PCL-HA、PCL-SF、PCL-GEL静电纺丝膜较PCL静电纺丝膜更有利于骨髓间充质干细胞的增殖、黏附,PCL-SF静电纺丝膜相较于PCL-HA、PCL-GEL静电纺丝膜更有利于骨髓间充质干细胞的增殖、黏附;③qRT-PCR检测显示,4种静电纺丝膜材料均可维持骨髓间充质干细胞CD90和Meflin的mRNA表达,组间比较差异无显著性意义(P>0.05);PCL-HA、PCL-SF、PCL-GEL组培养1,7 d的转化生长因子βmRNA表达高于PCL组(P<0.05),PCL-SF组培养3,7 d的转化生长因子βmRNA表达高于其他3组(P<0.05),PCL-HA组培养7 d的转化生长因子βmRNA表达高于PCL-GEL组(P<0.05);④qRT-PCR检测显示,PCL-SF组ɑ-平滑肌肌动蛋白mRNA表达高于其他3组(P<0.05),PCL-HA组ɑ-平滑肌肌动蛋白mRNA表达高于PCL组(P<0.05);⑤结果表明:相较于PCL、PCL-HA、PCL-GEL静电纺丝膜,PCL-SF静电纺丝膜与骨髓间充质干细胞结合更适合制备小口径组织工程血管。 展开更多
关键词 骨髓间充质干细胞 小口径组织工程血管 静电纺丝 聚己内酯 聚己内酯-透明质酸 聚己内酯-丝素蛋白 聚己内酯-明胶
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