Non-genotoxicity of Dental Monomer Siloranes in the Sister Chromatid Exchange Assay with Cultured Mammalian Cells

目的:Siloranes是一种含硅的新型牙科复合树脂环氧乙烷单体,较传统的含碳的环氧乙烷单体具有更好的生物相容性。本研究的目的为用哺乳动物细胞培养检测姐妹染色体交换的方法评估Siloranes的基因毒性,并与传统的环氧乙烷比较。方法:研究所用的传统环氧乙烷为CyracureTM UVR6105。Silorane包括Ph-Sil,TET-Sil和二者50/50(w/w)的混合物(3M-ESPE)。用CHO细胞在有或无S9情况下检测姐妹染色体交换(SCE)。Bonferron post hoc统计分析检测有无显著性差异。结果:与溶剂对照相比,在有或无S9情况下,环氧乙烷UVR6105显著性地增加了培养CHO细胞的SCE数目。3种Silorane单体Ph-Sil,TET-Sil或Sil-Mix均未能增加SCE数目。结论:用哺乳动物细胞培养检测姐妹染色体交换的方法评估显示,3种新型Silorane单体Ph-Sil,TET-Sil或Sil-Mix均未检测出基因毒性效果,提示Silorane单体具有较低基因毒性,是适于研发牙科树脂的新型单体。

Cytotoxicity of a silorane-based dental composite on human gingival fibroblasts

AIM:To evaluate the direct and indirect biocompatibility of Filtek Silorane on human gingival fibroblastic cells.METHODS:Sixty-three standardized cylindrical specimens(8 mm diameter and 2 mm thickness)of restorative material were prepared using a light emitting diode-curing unit.The sample were built up in one increment and divided in 2 groups.In the first group,21samples(unpolished samples)were left without a specific polishing procedure;in the second one,42 samples(polished samples)were polished with 4 different grains of discs.Fibroblast cultures,obtained from gingiva of 2 subjects without systemic and oral disease,were used to assess the direct and indirect biocompatibility.Cells cultured for 48 h in normal culture medium were used as a control.RESULTS:The scanning electron microscope observations of fibroblasts cultured on the silorane samples,either polished or unpolished,confirmed the good biocompatibility of the material,favouring the cellular spreading.3-dimethylthiazol-2,5-diphenyltetrazolium bromide tests showed a significant reduction(P<0.01)of gingival fibroblasts viability cultured both in polished samples(90.05%±19.00%)and unpolished samples(78.15%±11.00%)compared with the control.Cells growth in medium conditioned with the samples for 1 wk showed a significant viability reduction(P<0.01)compared to the control.A reduction of cell viability was observed even in the groups containing the material for 3 wk(polished:89.45%±10.00%;unpolished:65.97%±10.00%),even if the cytotoxicity was reduced after this long time exposure.CONCLUSION:Although the poor chromatic availability of this material remains a big limit that restricts its use to posterior sectors,the silorane-based material can be considered an option to perform restorations when aesthetic demands are not the priority,such as the

新型低聚合收缩后牙树脂P90临床应用效果的对比观察

目的观察新型低聚合收缩后牙树脂FiltekTMP90的临床疗效,并与传统后牙树脂FiltekTMP60相比较。方法将20名患者的同颌同名同洞型牙齿进行自身对照,一侧用P90树脂充填,一侧用P60树脂充填,于术后6个月、1年、2年复查,观察两种树脂的充填效果。结果 P90和P60充填体的2年成功率分别为90%和85%,差异无统计学意义。结论 P90和P60树脂的2年充填效果均令人满意。

纤维桩联合树脂覆盖牙尖充填修复磨牙大面积缺损的临床效果评价

目的:评价纤维桩联合树脂覆盖牙尖充填修复磨牙大面积缺损的临床效果。方法:选取2009年1月—2019年12月在本院诊治的磨牙大面积缺损患者63例,采用随机信封抽签法将其分为研究组和对照组。研究组32例、对照组31例。对照组给予常规纤维桩覆盖牙尖充填修复治疗,研究组给予纤维桩联合Silorane树脂覆盖牙尖充填修复治疗。结果:研究组术后3个月的磨牙松动度(0度、Ⅰ度、Ⅱ度、Ⅲ度分别为29例、3例、0例、0例)少于对照组(0度、Ⅰ度、Ⅱ度、Ⅲ度分别为19例、5例、4例、3例)(P<0.05)。研究组术后3个月的总有效率比对照组高,差异显著(P<0.05)。以手术前1 d作为参照时间,两组菌斑指数与牙龈指数在术后3个月明显减少,且研究组少于对照组,差异显著(P<0.05)。研究组术后3个月的根尖周病变、冠根折、桩体脱落、桩体松动等并发症发生率为6.3%,低于对照组的35.5%(P<0.05)。结论:纤维桩联合树脂覆盖牙尖充填修复磨牙大面积缺损能降低磨牙松动度,减少对菌斑指数与牙龈指数的影响,减少并发症的发生率。从而提高总体治疗效果。