Introduction Artificial multiple tetraploid allogynogenetic silver crucian carp possesses the reproductive function of gynogenesis in spite of incorporating one genome of common carp, because all chromosomes of silver...Introduction Artificial multiple tetraploid allogynogenetic silver crucian carp possesses the reproductive function of gynogenesis in spite of incorporating one genome of common carp, because all chromosomes of silver crucian carp are maintained. This was first confirmed in the artificial propagation experiments in which the eggs of the multiple tetraploid fish were respectively inseminated with normal and UV-irradiated sperms of red common carp. Recently,展开更多
1 Introduction Gynogenesis is a rare reproductive mode in fish. Among a few of species reproduced by this method, silver crucian carp (Carassius auratus gibelio) is the most special one. The specialities mainly includ...1 Introduction Gynogenesis is a rare reproductive mode in fish. Among a few of species reproduced by this method, silver crucian carp (Carassius auratus gibelio) is the most special one. The specialities mainly include the following two aspects: (ⅰ)The crucian carp exists as a bisexual population which can be reproduced by natural gynogenesis, because there are both gynogenetic function and certain proportion of males in the offsprings; (ⅱ)展开更多
A modified mRNA differential display method has been applied to studying differential expression of protein kinase genes in oocytes between natural gynogenetic silver crucian carp and amphimictic crucian carp. Total R...A modified mRNA differential display method has been applied to studying differential expression of protein kinase genes in oocytes between natural gynogenetic silver crucian carp and amphimictic crucian carp. Total RNA was reverse transcribed using downstream 3′primers T<sub>12</sub>MA, T<sub>12</sub>MG and T<sub>12</sub>MC respectively. Then the reverse transcription products were amplified using upstream 5′kinase-specific primer designed according to protein kinase conserved sequence. The PCR products had different patterns and numbers of cDNA bands on polyacrylamide gel. Totally 21 cDNAs fragments were recovered and cloned. Two of them were confirmed to be particularly expressed in oocytes of amphimictic crucian carp, and another was specific for gynogenetic silver crucian carp.展开更多
I Introduction Artificial multiple tetraploid allogynogenetic silver crucian carp is a special individual, which was discovered from artificially propagated populations of allogynogenetic silver crucian carp and has t...I Introduction Artificial multiple tetraploid allogynogenetic silver crucian carp is a special individual, which was discovered from artificially propagated populations of allogynogenetic silver crucian carp and has the obviously enhanced growth rates. One of its main specialities is that the fish maintains the whole chromosomes (162) of silver crucian carp (Carassius auratus gibelio) and fuses one chromosome set of haploid sperm (50 chromosomes ) of展开更多
The spindle behavior and MPF activity changes in the progression of oocyte maturation were investigated and compared with cytological observation and kinase assay between gynogenetic silver crucian carp and amphimicti...The spindle behavior and MPF activity changes in the progression of oocyte maturation were investigated and compared with cytological observation and kinase assay between gynogenetic silver crucian carp and amphimictic colored crucian carp. MPF activity was measured by using histone H1 as phosphorylation substrate. There were two similar oscillatory MPF kinase activity changes during oocyte maturation in two kinds of fishes with different reproductive modes, but there existed some subtle difference between them. The subtle difference was that the first peak of MPF kinase activity was kept to a longerlasting time in the gynogenetic silver crucian carp than in the amphimictic colored crucian carp. It was suggested that the difference may be related to the spindle behavior changes, such as tripolar spindle formation and spindle rearrangement in the gynogenetic crucian carp.展开更多
Genetic homogeneity between Pengze crucian carp and strain A of silver crucian carp was studied by using transferrin, isozyme and RAPD markers. The studied individuals of Pengze crucian carp showed transferring patter...Genetic homogeneity between Pengze crucian carp and strain A of silver crucian carp was studied by using transferrin, isozyme and RAPD markers. The studied individuals of Pengze crucian carp showed transferring patterns were the same of silver crucian carp A strain while distinct from those of other crucian carp populations. As far as isozyme is concerned, the MDH, LDH and EST are all of the same with only slight differences in SOD between them. The RPAD patterns clearly indicated high homogeneity among 16 individuals(6 sampled from individuals of two years old and the others aged one) from crucian carp of Pengze and 5 individuals from strain A of silver crucain carp. Nearly indentical banding patterns were observed among all individuals. Average genetic distance within all the individuals is only 0.011, suggesting crucian carp of Pengze might possess indentical genetic background with strain A of silver crucian carp.展开更多
文摘Introduction Artificial multiple tetraploid allogynogenetic silver crucian carp possesses the reproductive function of gynogenesis in spite of incorporating one genome of common carp, because all chromosomes of silver crucian carp are maintained. This was first confirmed in the artificial propagation experiments in which the eggs of the multiple tetraploid fish were respectively inseminated with normal and UV-irradiated sperms of red common carp. Recently,
基金Project supported by the president grant from Academia Sinica.
文摘1 Introduction Gynogenesis is a rare reproductive mode in fish. Among a few of species reproduced by this method, silver crucian carp (Carassius auratus gibelio) is the most special one. The specialities mainly include the following two aspects: (ⅰ)The crucian carp exists as a bisexual population which can be reproduced by natural gynogenesis, because there are both gynogenetic function and certain proportion of males in the offsprings; (ⅱ)
文摘A modified mRNA differential display method has been applied to studying differential expression of protein kinase genes in oocytes between natural gynogenetic silver crucian carp and amphimictic crucian carp. Total RNA was reverse transcribed using downstream 3′primers T<sub>12</sub>MA, T<sub>12</sub>MG and T<sub>12</sub>MC respectively. Then the reverse transcription products were amplified using upstream 5′kinase-specific primer designed according to protein kinase conserved sequence. The PCR products had different patterns and numbers of cDNA bands on polyacrylamide gel. Totally 21 cDNAs fragments were recovered and cloned. Two of them were confirmed to be particularly expressed in oocytes of amphimictic crucian carp, and another was specific for gynogenetic silver crucian carp.
文摘I Introduction Artificial multiple tetraploid allogynogenetic silver crucian carp is a special individual, which was discovered from artificially propagated populations of allogynogenetic silver crucian carp and has the obviously enhanced growth rates. One of its main specialities is that the fish maintains the whole chromosomes (162) of silver crucian carp (Carassius auratus gibelio) and fuses one chromosome set of haploid sperm (50 chromosomes ) of
文摘The spindle behavior and MPF activity changes in the progression of oocyte maturation were investigated and compared with cytological observation and kinase assay between gynogenetic silver crucian carp and amphimictic colored crucian carp. MPF activity was measured by using histone H1 as phosphorylation substrate. There were two similar oscillatory MPF kinase activity changes during oocyte maturation in two kinds of fishes with different reproductive modes, but there existed some subtle difference between them. The subtle difference was that the first peak of MPF kinase activity was kept to a longerlasting time in the gynogenetic silver crucian carp than in the amphimictic colored crucian carp. It was suggested that the difference may be related to the spindle behavior changes, such as tripolar spindle formation and spindle rearrangement in the gynogenetic crucian carp.
文摘Genetic homogeneity between Pengze crucian carp and strain A of silver crucian carp was studied by using transferrin, isozyme and RAPD markers. The studied individuals of Pengze crucian carp showed transferring patterns were the same of silver crucian carp A strain while distinct from those of other crucian carp populations. As far as isozyme is concerned, the MDH, LDH and EST are all of the same with only slight differences in SOD between them. The RPAD patterns clearly indicated high homogeneity among 16 individuals(6 sampled from individuals of two years old and the others aged one) from crucian carp of Pengze and 5 individuals from strain A of silver crucain carp. Nearly indentical banding patterns were observed among all individuals. Average genetic distance within all the individuals is only 0.011, suggesting crucian carp of Pengze might possess indentical genetic background with strain A of silver crucian carp.
