The COVID-19 pandemic has brought great challenges to traditional nucleic acid detection technology.Thus,it is urgent to develop a more simple and efficient nucleic acid detection technology.CRISPR-Cas12 has signal am...The COVID-19 pandemic has brought great challenges to traditional nucleic acid detection technology.Thus,it is urgent to develop a more simple and efficient nucleic acid detection technology.CRISPR-Cas12 has signal amplification ability,high sensitivity and high nucleic acid recognition specificity,so it is considered as a nucleic acid detection tool with broad development prospects and high application value.This review paper discusses recent advances in CRISPR-Cas12-based nucleic acid detection,with an emphasis on the new research methods and means to improve the nucleic acid detection capability of CRISPR-Cas12.Strategies for improving sensitivity,optimization of integrated detection,development of sim-plified detection mode and improvement of quantitative detection capabilities are included.Finally,the future development of CRISPR-Cas12-based nucleic acids detection is prospected.展开更多
基金supported by the National Natural Science Foundation of China(91959128,21874049).
文摘The COVID-19 pandemic has brought great challenges to traditional nucleic acid detection technology.Thus,it is urgent to develop a more simple and efficient nucleic acid detection technology.CRISPR-Cas12 has signal amplification ability,high sensitivity and high nucleic acid recognition specificity,so it is considered as a nucleic acid detection tool with broad development prospects and high application value.This review paper discusses recent advances in CRISPR-Cas12-based nucleic acid detection,with an emphasis on the new research methods and means to improve the nucleic acid detection capability of CRISPR-Cas12.Strategies for improving sensitivity,optimization of integrated detection,development of sim-plified detection mode and improvement of quantitative detection capabilities are included.Finally,the future development of CRISPR-Cas12-based nucleic acids detection is prospected.
