The changes of malondialdehyde （MDA）, H2O2, and O2^7 content, or the activities of superoxide dismutase （SOD）, catalase （CAT）, ascrobate peroxidase （APX）, peroxidase （POD）, phenylalanine ammonia lyase （PAL...The changes of malondialdehyde （MDA）, H2O2, and O2^7 content, or the activities of superoxide dismutase （SOD）, catalase （CAT）, ascrobate peroxidase （APX）, peroxidase （POD）, phenylalanine ammonia lyase （PAL）, and polyphenol oxidase （PPO） in pea seedlings （Pisum sativum L.） under wounding and treatment of exogenous jasmonic acid （JA） were investigated. The results showed that the activities of both phenylalanine ammonia lyase （PAL） and polyphenol oxidase （PPO） were significantly increased by wounding and application of JA. The metabolism of reaction oxidative species （ROS） was enhanced, especially O2^7 and H2O2 appeared to rapidly increase. The activities of antioxidant enzymes such as SOD, CAT, APX and POD were also increased. Treatment of JA of 1 or 10 μmol L^-1 could effectively induce plant defense response, and thus decrease the peroxidation of cell membrane lipid. However, high concentration of JA （100 μmol L^-1） resulted in unbalance of metabolism of ROS and promoted the peroxidation of cell membrane lipid. We thus suggested that JA, under the suitable concentration, could induce defense response of pea seedlings to wounding.展开更多
Malus hupehensis was used as experiment materials.This experiments were conducted to explore the effects of benzoic acid on the productivity of superoxide（O2 ·）,the contents of malondialdehvde（MDA）and sulfhyd...Malus hupehensis was used as experiment materials.This experiments were conducted to explore the effects of benzoic acid on the productivity of superoxide（O2 ·）,the contents of malondialdehvde（MDA）and sulfhydryl group activity（—SH）,antioxidant enzyme activity and mineral elements in roots of M.hupehensis seedlings.The results showed that there was no significant difference between the treatment of 5 mg·kg-1（soil）benzoic acid and CK in above parameters.With 25 mg·kg-1（soil）and 125 mg·kg-1（soil）benzoic acid treatments,O2 · and MDA contents increased in the early period and then declined;—SH content decreased during the treatment time;activities of SOD and POD increased firstly,then decreased,CAT activity decreased firstly and then slightly reversed,but lower than that of CK;SOD,POD and CAT had a similar trend,activities of roots increased under 5 mg·kg-1（soil）benzoic acid,while they were inhibited by 25 mg·kg-1（soil）and 125 mg·kg-1（soil）benzoic acid;mineral elements were decreased.Benzoic acid in low concentration promoted antioxidant enzyme activity,—SH content,and uptake of mineral elements,reduced O2 · and MDA contents.In conclusion,benzoic acid of 5 mg·kg-1（soil）had no effects on Membrance Lipid Peroxidation and Antioxidant enzymes in roots of M.hupehensis seedlings;at 25 mg·kg-1（soil）and 125 mg·kg-1（soil）,benzoic acid increased Membrance Lipid Peroxidationd,decreased activity of antioxidant enzymes of roots,and damaged integrity and stability of cell membrane in M.hupehensis seedlings,meanwhile,contents of mineral elements were decreased.展开更多
基金the National Natural Sci-ence Foundation of China(30270918) the National High Technology Research and Development Programof China(863 Program2003AA241170).
文摘The changes of malondialdehyde （MDA）, H2O2, and O2^7 content, or the activities of superoxide dismutase （SOD）, catalase （CAT）, ascrobate peroxidase （APX）, peroxidase （POD）, phenylalanine ammonia lyase （PAL）, and polyphenol oxidase （PPO） in pea seedlings （Pisum sativum L.） under wounding and treatment of exogenous jasmonic acid （JA） were investigated. The results showed that the activities of both phenylalanine ammonia lyase （PAL） and polyphenol oxidase （PPO） were significantly increased by wounding and application of JA. The metabolism of reaction oxidative species （ROS） was enhanced, especially O2^7 and H2O2 appeared to rapidly increase. The activities of antioxidant enzymes such as SOD, CAT, APX and POD were also increased. Treatment of JA of 1 or 10 μmol L^-1 could effectively induce plant defense response, and thus decrease the peroxidation of cell membrane lipid. However, high concentration of JA （100 μmol L^-1） resulted in unbalance of metabolism of ROS and promoted the peroxidation of cell membrane lipid. We thus suggested that JA, under the suitable concentration, could induce defense response of pea seedlings to wounding.
文摘Malus hupehensis was used as experiment materials.This experiments were conducted to explore the effects of benzoic acid on the productivity of superoxide（O2 ·）,the contents of malondialdehvde（MDA）and sulfhydryl group activity（—SH）,antioxidant enzyme activity and mineral elements in roots of M.hupehensis seedlings.The results showed that there was no significant difference between the treatment of 5 mg·kg-1（soil）benzoic acid and CK in above parameters.With 25 mg·kg-1（soil）and 125 mg·kg-1（soil）benzoic acid treatments,O2 · and MDA contents increased in the early period and then declined;—SH content decreased during the treatment time;activities of SOD and POD increased firstly,then decreased,CAT activity decreased firstly and then slightly reversed,but lower than that of CK;SOD,POD and CAT had a similar trend,activities of roots increased under 5 mg·kg-1（soil）benzoic acid,while they were inhibited by 25 mg·kg-1（soil）and 125 mg·kg-1（soil）benzoic acid;mineral elements were decreased.Benzoic acid in low concentration promoted antioxidant enzyme activity,—SH content,and uptake of mineral elements,reduced O2 · and MDA contents.In conclusion,benzoic acid of 5 mg·kg-1（soil）had no effects on Membrance Lipid Peroxidation and Antioxidant enzymes in roots of M.hupehensis seedlings;at 25 mg·kg-1（soil）and 125 mg·kg-1（soil）,benzoic acid increased Membrance Lipid Peroxidationd,decreased activity of antioxidant enzymes of roots,and damaged integrity and stability of cell membrane in M.hupehensis seedlings,meanwhile,contents of mineral elements were decreased.
