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Changes in Antioxidant Activity of Alcalase-Hydrolyzed Soybean Hydrolysate under Simulated Gastrointestinal Digestion and Transepithelial Transport 被引量:1
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作者 Zhang Qiaozhi Tong Xiaohong +4 位作者 Qi Baokun Wang Zhongjiang Li Yang Sui Xiaonan Jiang Lianzhou 《大豆科技》 2019年第S01期84-96,共13页
Peptides from Alcalase-hydrolyzed soybean protein hydrolysate(SPH)may hold the potential as natural antioxidants.In addition,the effect of human gastrointestinal(GI)tract on peptide bioavailability needs to be explore... Peptides from Alcalase-hydrolyzed soybean protein hydrolysate(SPH)may hold the potential as natural antioxidants.In addition,the effect of human gastrointestinal(GI)tract on peptide bioavailability needs to be explored.In this study,the impact of simulated GI digestion and transepithelial transport on various antioxidant properties of SPH were investigated.SPH displayed DPPH radical scavenging(IC50=4.22 mg/m L),ABTS·+radical scavenging(IC50=2.93 mg/m L),reducing power and metal ion-chelating activities(IC50=0.67 mg/m L).Furthermore,SPH significantly(P<0.05)inhibited the generation of intracellular reactive oxygen species(ROS)in Caco-2 cells.After simulated GI digestion,the antioxidant properties of SPH were enhanced,except for a decrease in ABTS·+radical scavenging activity.After transepithelial transport,the permeates maintained partial antioxidant activity and the LC-MS/MS data further identified the absorbed soybean peptides.These results suggest that SPH contains the antioxidant peptides that are potentially bioavailable and can be regarded as a promising source of functional food ingredients. 展开更多
关键词 Soybean protein hydrolysate Antioxidant activity simulated gastrointestinal digestion Transepithelial transport Caco-2 cells
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Herba Epimedii′s biotransformation in vitro simulated gastrointestinal digestion and faecal fermentation systems
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作者 LI Ben ZHANG Xiao-yao +1 位作者 Alberto CP DIAS ZHANG Xiao-ying 《中国药理学与毒理学杂志》 CAS 北大核心 2021年第10期732-732,共1页
OBJECTIVE Epimedium is rich in a variety of beneficial active ingredients,and has been widely used in the ethnopharmacological practices,however,its biotransformation in gastrointestinal digestions remain unclear.This... OBJECTIVE Epimedium is rich in a variety of beneficial active ingredients,and has been widely used in the ethnopharmacological practices,however,its biotransformation in gastrointestinal digestions remain unclear.This study aimed to investigate the dynamic changes of components and biological activity of Epimedium in the in vitro simulated digestion and subsequent human faecal fermentation.METHODS The models of in vitro simulated saliva,gastric and intestinal digestion,as well as colonic fermentation were constructed to simulate the digestion process of Epimedium.The dynamic changes of components of Epimedium during the simulated digestions in vitro and subsequent human faecal fermentation were investigated by UPLC-MS,HPLC-DAD combined with principal component analysis(PCA)and multi-ingredient quantitative analysis.RESULTS A variety of metabolites with high contents were produced after 0.5 h of intestinal digestion and colonic fermentation 0.5 h.Application of PCA to HPLC data showed the obvious separation of colonic fermentation 0.5 h stage samples from other colonic fermentation stages samples(24,48 and 72 h).Additionally,non-digestion and saliva digestion stage samples clustered together,and there was obvious separation between intestinal digestion samples and gastric digestion samples.The contents of epimedium C,icariin and baohuside I all increased significantly after intestinal digestion[58.70±7.08,47.15±5.68 and(12.78±0.55)mg·g^(-1)]compared with gastric digestion[29.00±5.65,17.40±4.55 and(2.77±0.19)mg·g^(-1)].There were significant differences between sample after 0.5 h of colonic fermentation[64.22±9.32,51.26±6.33 and(16.68±3.19)mg·g^(-1)]and other time points(24,48 and 72 h)in components and the contents of active ingredient,and the content of these components all decreased with the fermentation time.The ability of scavenging ABTS free radicals[IC50=(0.29±0.02)g·L^(-1)]increased significantly compared with gastric digestion[(1.57±0.02)g·L^(-1)],and after 0.5 h of colonic fermentation,the ability also increased significantly.CONCLUSION Gastrointestinal digestion had a significant impact on the contents of active components in Epimedium,and the metabolism of these components mainly occurred in the colon.The intestinal digestion and colonic fermentation significantly improved the anti-ABTS activity of epimedium. 展开更多
关键词 EPIMEDIUM simulated gastrointestinal digestion faecal fermentation BIOTRANSFORMATION
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Microencapsulation of immunoglobulin Y: optimization with response surface morphology and controlled release during simulated gastrointestinal digestion 被引量:3
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作者 Jin ZHANG Huan-huan LI +7 位作者 Yi-fan CHEN Li-hong CHEN Hong-gang TANG Fan-bin KONG Yun-xin YAO Xu-ming LIU Qian LAN Xiao-fan YU 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE CAS CSCD 2020年第8期611-627,共17页
Immunoglobulin Y(Ig Y)is an effective orally administered antibody used to protect against various intestinal pathogens,but which cannot tolerate the acidic gastric environment.In this study,Ig Y was microencapsulated... Immunoglobulin Y(Ig Y)is an effective orally administered antibody used to protect against various intestinal pathogens,but which cannot tolerate the acidic gastric environment.In this study,Ig Y was microencapsulated by alginate(ALG)and coated with chitooligosaccharide(COS).A response surface methodology was used to optimize the formulation,and a simulated gastrointestinal(GI)digestion(SGID)system to evaluate the controlled release of microencapsulated Ig Y.The microcapsule formulation was optimized as an ALG concentration of 1.56%(15.6 g/L),COS level of 0.61%(6.1 g/L),and Ig Y/ALG ratio of 62.44%(mass ratio).The microcapsules prepared following this formulation had an encapsulation efficiency of 65.19%,a loading capacity of 33.75%,and an average particle size of 588.75μm.Under this optimum formulation,the coating of COS provided a less porous and more continuous microstructure by filling the cracks on the surface,and thus the GI release rate of encapsulated Ig Y was significantly reduced.The release of encapsulated Ig Y during simulated gastric and intestinal digestion well fitted the zero-order and first-order kinetics functions,respectively.The microcapsule also allowed the Ig Y to retain 84.37%immune-activity after 4 h simulated GI digestion,significantly higher than that for unprotected Ig Y(5.33%).This approach could provide an efficient way to preserve Ig Y and improve its performance in the GI tract. 展开更多
关键词 Immunoglobulin Y(IgY) MICROENCAPSULATION Chitooligosaccharide(COS) Response surface methodology(RSM) Controlled release simulated gastrointestinal digestion(SGID)
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Evaluation of viability to simulated gastrointestinal tract passage of probiotic strains and pioneer bioaccessibility analyses of antioxidants in chocolate
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作者 Jocelane Zoldan Ivan De Marco +4 位作者 Silvani Verruck Aline Iamin Gomide Claudio Eduardo Leite Cartabiano Gilberto Vinicius de Melo Pereira Juliano De Dea Lindner 《Food Bioscience》 SCIE 2023年第2期1258-1267,共10页
Chocolate is appreciated worldwide for its flavor and antioxidant properties.This study aims to evaluate the viability of Lactiplantibacillus plantarum,Lacticaseibacillus casei,and Bacillus subtilis in milk and 70%coc... Chocolate is appreciated worldwide for its flavor and antioxidant properties.This study aims to evaluate the viability of Lactiplantibacillus plantarum,Lacticaseibacillus casei,and Bacillus subtilis in milk and 70%cocoa chocolates during 90-days.Chocolates incorporated with free and microencapsulated strains were subjected to a gastrointestinal tract(GIT)in vitro passage simulation.Results indicate that survival depends on the strain used;B.subtilis presented the highest performance,with values above 8.0 log CFU/g.GIT simulation demonstrated strains survival during the passage through all the phases,with intestinal phase average values above 7.9 log CFU/g for B.subtilis and L.casei;a factor attributed to the protection provided by the chocolate matrix.Bioaccessibility to phenolics,flavonoids,and antioxidants was also higher in all samples simulated analysis,and 70%cocoa chocolates showed the highest concentrations.The chocolates showed high acceptance in the sensorial analysis.The maintenance of the viability of potential probiotic microorganisms in the food matrix,and the high content of antioxidant compounds,indicate the great potential of chocolate for the diversification of probiotic foods currently available in the market. 展开更多
关键词 Cell viability gastrointestinal simulation Antioxidant activity Sensory analysis Chocolate Probiotics
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Purifi cation, characterization and hypoglycemic activity of glycoproteins obtained from pea (Pisum sativum L.) 被引量:10
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作者 Gaoyixin Qin Wu Xu +2 位作者 Junping Liu Liyan Zhao Guitang Chen 《Food Science and Human Wellness》 SCIE 2021年第3期297-307,共11页
This study aimed to isolate and characterize the structures of glycoproteins from peas and determine their hypoglycemic activity.The crude pea glycoproteins(PGP)were extracted by hot water and purified by diethylamino... This study aimed to isolate and characterize the structures of glycoproteins from peas and determine their hypoglycemic activity.The crude pea glycoproteins(PGP)were extracted by hot water and purified by diethylaminoethyl(DEAE)-Sepharose chromatography and Sephadex G-100 size-exclusion chromatography in sequence.Then three main fractions were obtained,namely PGP1,PGP2 and PGP3,with molecular weights of 897615,846740 and 1194692 Da,respectively.The physical and chemical properties of the three fractions were evaluated and compared by Fourier transform infrared spectroscopy(FT-IR),nuclear magnetic resonance(NMR),scanning electron microscope(SEM),high performance liquid chromatography(HPLC)and other analytical techniques.The fraction PGP2 with the highest hypoglycemic activity,was screened using the Caco-2 monolayer cell model.It can inhibit the uptake of glucose in the small intestine,as well as the activities of maltase and sucrase.After simulated gastrointestinal digestion,PGP2 signifi cantly enhanced the inhibitory effect of α-glucosidase,and slightly reduced the inhibitory ability ofα-amylase.In summary,PGP2 possessed strong hypoglycemic activity after digestion.These results indicated that PGP2 has the potential to be developed into a functional food or natural medicine for the treatment of type 2 diabetes mellitus. 展开更多
关键词 Pea(Pisum sativum L.) GLYCOPROTEIN Structural characterization Hypoglycemic activity simulated gastrointestinal digestion
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The dual-function of bioactive peptides derived from oyster (Crassostrea gigas) proteins hydrolysates
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作者 Dongyang Zhu Zhen Yuan +3 位作者 Di Wu Chao Wu Hesham R.El-Seedi Ming Du 《Food Science and Human Wellness》 SCIE CSCD 2023年第5期1609-1617,共9页
Oysters(Crassostrea gigas)have a wide range of functionality due to their nutritional and bioactive components. However, the bioactive peptides of oyster proteins are rarely reported, particularly their antidiabetes e... Oysters(Crassostrea gigas)have a wide range of functionality due to their nutritional and bioactive components. However, the bioactive peptides of oyster proteins are rarely reported, particularly their antidiabetes effects and antioxidants. Oyster proteins were extracted from fresh oysters using phosphatebuffered saline and simulated gastrointestinal digestion was performed. The degree of hydrolysis(DH), structural characterization, molecular weight(Mw)distribution, free amino acid, anti-diabetic activity, and antioxidant activity were studied during in vitro simulated gastrointestinal digestion. The results showed that the α-glucosidase inhibitory activity, α-amylase inhibitory activity, DPPH radical scavenging activity, and ABTS radical scavenging activity of the oyster protein gastrointestinal digest were increased(P < 0.05)from 0 to 33.96%, from 9.17% to 44.22%, from 9.01 μg trolox/mg protein to 18.48 μg trolox/mg protein, and from 21.44 μg trolox/mg protein to 56.21 μg trolox/mg protein, respectively. Additionally, the DH, β-turn structure, fluorescence intensity, free amino acid, and short peptide content(Mw < 1 000 Da)increased in the simulated gastrointestinal digestion. These results indicate that the digestive hydrolysates obtained from oyster proteins could be used as natural anti-diabetic and antioxidant agents. 展开更多
关键词 Oyster proteins simulated gastrointestinal digestion Anti-diabetic activity Antioxidant activity Free amino acid
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Development of milk powder containing Lactobacillus plantarum NCIMB 8826 immobilized with prebiotic hi-maize starch and survival under simulated gastric and intestinal conditions
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作者 Author detais Ronamae Bradford +6 位作者 Vondel Reyes Franklin Bonilla Federico Bueno Bennett Dzandu Chen Liu Alexander Chouljenko Subramaniam Sathivel 《Food Production, Processing and Nutrition》 2019年第1期36-43,共8页
The objectives of this study were to develop a probiotic milk powder containing Lactobacillus plantarum NCIMB 8826 immobilized with prebiotic Hi-maize starch and to analyze cell viability after spray drying and exposu... The objectives of this study were to develop a probiotic milk powder containing Lactobacillus plantarum NCIMB 8826 immobilized with prebiotic Hi-maize starch and to analyze cell viability after spray drying and exposure to simulated gastric and intestinal conditions.Milk powders containing free L.plantarum and cells immobilized with Hi-maize starch were assessed.Powders were evaluated during storage at 4°C for 15 days.After spray drying,at 0 and 15 days of storage both treatments had over 8 log CFU/g of viable cells and there were higher viable counts found for immobilized cells compared to free cells after 120 min in simulated gastric fluid.At 15 days of storage,immobilized cells had higher viable counts than free cells after exposure to simulated intestinal fluid for 120 min.The combined probiotic and prebiotic milk powder had stable viable cell counts at refrigerated storage conditions and under simulated gastric and intestinal transit. 展开更多
关键词 PROBIOTICS PREBIOTICS simulated gastrointestinal conditions Milk powder Spray drying Immobilization
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Preparation, characterization and gastrointestinal stability of silk fibroin nanoparticles loaded with red wine polyphenols
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作者 Diego Paladines-Quezada Carolina Cueva +4 位作者 Rocio Gil-Munoz Jose Luis Cenis Begona Bartolome M.Victoria Moreno-Arribas Antonio Abel Lozano-Perez 《Food Bioscience》 SCIE 2023年第2期1091-1101,共11页
Silk fibroin nanoparticles(SFN)are interesting drug delivery systems due to their high load capacity and low toxicity.The aim of this study was to reveal how SFN load red wine polyphenols(RWP),and to assess their prot... Silk fibroin nanoparticles(SFN)are interesting drug delivery systems due to their high load capacity and low toxicity.The aim of this study was to reveal how SFN load red wine polyphenols(RWP),and to assess their protective effect during the early stages of digestion by using the dynamic gastrointestinal simulator(simgi^(R))under physiological conditions.RWP-SFN were prepared by incubation of SFN in red wine and recovered by centrifugation.The obtained nanoparticles(particle size of 194.4±2.1 nm and a Zeta potential of􀀀22.7±0.1 mV)showed a significant polyphenol loading content(7386.59±1018.28μg/g)with a high encapsulation efficiency(66.66±2.58%).They were highly stable during their passage through the digestive tract,being the activity of RWP highly preserved.This data paves the way for future developments of SFN as stabilizers with a wide range of applications in the field of food additives. 展开更多
关键词 Silk fibroin nanoparticles Wine polyphenols Adsorption Dynamic gastrointestinal simulator(simgi^(R)) Protein digestion
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Effects of Hericium erinaceus polypeptide on lowering blood lipids of mice with hyperlipidemia induced by a high-fat diet 被引量:2
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作者 Nan Wang Zhengquan Tong +2 位作者 Dawei Wang Yanrong Zhang Tingting Liu 《Journal of Future Foods》 2022年第4期346-357,共12页
Hericium erinaceus polypeptide(HEP)was prepared by an ultrasound-microwave assisted enzymatic method.Using an ultrafiltration membrane with molecular weights of 5 and 10 kDa,HEP was fractionated into three fractions,n... Hericium erinaceus polypeptide(HEP)was prepared by an ultrasound-microwave assisted enzymatic method.Using an ultrafiltration membrane with molecular weights of 5 and 10 kDa,HEP was fractionated into three fractions,namely,(HEP-I(<5 kDa),HEP-II(5–10 kDa),and HEP-III(>10 kDa)).In vitro chemical methods were used to compare the antioxidant and hypolipidemic abilities of the polypeptide fractions from H.erinaceus before and after simulated gastrointestinal digestion.By constructing a hyperlipidemia model,the hypolipidemic ability of the high active fraction(HEP-II)was verified.The results showed that the antioxidant and hypolipidemic abilities of the polypeptide fractions from H.erinaceus did not change dramatically during simulated gastrointestinal digestion in vitro.The polypeptide fractions from H.erinaceus exhibited high tolerance to simulated gastrointestinal digestion in vitro,with strong antioxidant and hypolipidemic activities.HEP-II with a molecular weight of 5–10 kDa had the best stability,antioxidant,and hypolipidemic abilities in gastrointestinal digestion.The secondary structure of HEP-II was mainly composed of random coil(18.36%)andα-helix(47.71%)structures,which was beneficial to the hypolipidemic ability of HEP-II.Animal experiments showed that compared to the high-fat model group,HEP-II could inhibit the weight gain of the mice,decrease the liver index and serum levels of the serum total cholesterol(TC),triglycerides(TG),low-density lipoprotein cholesterol(LDL-C),nitric oxide synthase(NOS),alanine aminotransferase(AST),and glutamic-pyruvic transaminase(ALT),increase the levels of glutathione peroxidase(GSH-Px)and high-density lipoprotein cholesterol(HDL-C),decrease the arteriosclerosis index(AI),and improve the hemorheological indices of the mice.In addition,the whole blood and plasma viscosities of the mice decreased,and HEP-II increased the level of superoxide dismutase(SOD)in the liver,reducing the level of malondialdehyde(MDA),and the degree of oxidative stress in the liver of hypolipidemia mice.Furthermore,HEP-II improved liver steatosis.These results indicated that the polypeptide fractions from H.erinaceus all had a potential hypolipidemic effect,and HEP-II had the strongest potential hypolipidemic effect. 展开更多
关键词 Hericium erinaceus polypeptide simulated gastrointestinal digestion Structure characterization ANTIOXIDANT Aypolipidemia
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Peptides-carrageenan-xanthan gum:Printing mechanism and anti-oxidation under in vitro digestion
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作者 Jun-qi Zhan Wen-tao Yu +3 位作者 Jing-jing Fu Gao-shang Li Ya-qin Hu Yue-wen Chen 《Food Bioscience》 SCIE 2023年第3期1802-1812,共11页
Surimi is a protein-rich food.The peptides hydrolyzed by surimi have many functional activities and showed strong antioxidant activity in ABTS free radical scavenging experiments,hydroxyl radical scavenging experiment... Surimi is a protein-rich food.The peptides hydrolyzed by surimi have many functional activities and showed strong antioxidant activity in ABTS free radical scavenging experiments,hydroxyl radical scavenging experiments and iron ion reduction experiments.The scavenging rates of ABTS ions,hydroxyl radicals and iron ions were 62.83%,74.81%and 0.035 mg/mL,respectively.3D printing ink was formulated by compounding carrageenan,xanthan gum and surimi peptides.The surimi polypeptide“ink”contains 3%carrageenan,4%xanthan gum and 4 mg/mL surimi polypeptide.This“ink”not only has good printability,but also has stable antioxidant properties.The effect of the peptides on the printed structures can also be observed under an inverted fluorescence microscope.In the in vitro digestion experiment,the surimi polypeptide 3D printing ink added with carrageenan and xanthan gum can release most of the peptides.After in vitro digestion experiments,the antioxidant activity of surimi polypeptides increased.The above experiments have proved that surimi polypeptide is a high-quality raw material for preparing 3D printing ink. 展开更多
关键词 Peptide inks 3D printing Structure simulated gastrointestinal digestion Anti-oxidation properties
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