An efficient in vitro protocol for mass production of shoot of Dahlia was developed by using node explant, various carbon sources such as sucrose, glucose, fructose and galactose. Agar concentrations and various growt...An efficient in vitro protocol for mass production of shoot of Dahlia was developed by using node explant, various carbon sources such as sucrose, glucose, fructose and galactose. Agar concentrations and various growth regulators on in vitro shoot multiplication of Dahlia were studied in the present investigation. The nodal explant from the gardens grown plant were used as testing plant material to develop an efficient protocol for mass propagation of exotic Dahlia to enhance their production for growers and the local markets. This study determined the effect of different carbon sucrose concentrations and gelling agent on in vitro propagation of Dahlia, different carbon sources （sucrose, glucose, fructose and galactose） were investigated, each sugar was added individually to the MS culture medium at the concentrations of 15, 30 and 45 g·L^-1, respectively. Culture medium of each treatment was supplemented with 1,5 mg·L^-1 BA ＋ 1.5 mg·L^-1Kin ＋ 7,0 g·L^-1 agar. The highest number of shoots （7.00）, number of leaves （11.50）, number of node （6.75） and shoot length （8.24 cm） was obtained on MS medium supplemented with 30 g·L^-1 glucose. The least number of shoots （3.38）, number of leaves （5.00）, number of node （3.13） and the least shoot length （2.96 cm） was obtained on 45 g·L^-1 galactose and the least shoot length （2.29 cm） was observed on MS medium with free carbon sources. While the medium with 30 g·L^-1 glucose and 8 g·L^-1 agar gave the highest number of shoots （7.13）, number of leaves （10.75）, number of node （7.13） and shoot length （8.18 cm）. However, the least number of shoots （1.50）, number of leaves （1.88）, number of node （1.63） and the least shoot length （1.26 cm） was obtained with 30 g·L^-1 galactose and 12 g·L^-1 agar. Rooting was readily achieved upon transferring the microshoots onto MS medium supplemented with 0.1 mg·L^-1 IBA, IAA and NAA and 30 g·L^-1 （w/v） different types of carbon sources. The percentage of rooting was less （71.88%） on MS medium containing IAA as compared with IBA or NAA. While the medium having 30 g·L^-1 glucose with 0.1 IBA or NAA mg·L^-1, give the highest percentage of root （100%）, and the highest number of root （3.88） and root length （3.56 cm） was obtained on MS medium containing 30 g·L^-1 glucose with 0.1 mg·L^-1 IBA. More than 98% of rooted plantlets were established in the greenhouse.展开更多
文摘An efficient in vitro protocol for mass production of shoot of Dahlia was developed by using node explant, various carbon sources such as sucrose, glucose, fructose and galactose. Agar concentrations and various growth regulators on in vitro shoot multiplication of Dahlia were studied in the present investigation. The nodal explant from the gardens grown plant were used as testing plant material to develop an efficient protocol for mass propagation of exotic Dahlia to enhance their production for growers and the local markets. This study determined the effect of different carbon sucrose concentrations and gelling agent on in vitro propagation of Dahlia, different carbon sources （sucrose, glucose, fructose and galactose） were investigated, each sugar was added individually to the MS culture medium at the concentrations of 15, 30 and 45 g·L^-1, respectively. Culture medium of each treatment was supplemented with 1,5 mg·L^-1 BA ＋ 1.5 mg·L^-1Kin ＋ 7,0 g·L^-1 agar. The highest number of shoots （7.00）, number of leaves （11.50）, number of node （6.75） and shoot length （8.24 cm） was obtained on MS medium supplemented with 30 g·L^-1 glucose. The least number of shoots （3.38）, number of leaves （5.00）, number of node （3.13） and the least shoot length （2.96 cm） was obtained on 45 g·L^-1 galactose and the least shoot length （2.29 cm） was observed on MS medium with free carbon sources. While the medium with 30 g·L^-1 glucose and 8 g·L^-1 agar gave the highest number of shoots （7.13）, number of leaves （10.75）, number of node （7.13） and shoot length （8.18 cm）. However, the least number of shoots （1.50）, number of leaves （1.88）, number of node （1.63） and the least shoot length （1.26 cm） was obtained with 30 g·L^-1 galactose and 12 g·L^-1 agar. Rooting was readily achieved upon transferring the microshoots onto MS medium supplemented with 0.1 mg·L^-1 IBA, IAA and NAA and 30 g·L^-1 （w/v） different types of carbon sources. The percentage of rooting was less （71.88%） on MS medium containing IAA as compared with IBA or NAA. While the medium having 30 g·L^-1 glucose with 0.1 IBA or NAA mg·L^-1, give the highest percentage of root （100%）, and the highest number of root （3.88） and root length （3.56 cm） was obtained on MS medium containing 30 g·L^-1 glucose with 0.1 mg·L^-1 IBA. More than 98% of rooted plantlets were established in the greenhouse.
