通过不同甘蓝型油菜中SLG基因的克隆及序列分析,探索了甘蓝型油菜中是否存在与芸薹属二倍体中相同或相似的S-locus基因。对10个甘蓝型油菜品种和品系中SLG基因的PCR扩增和序列比较分析发现,这些甘蓝型油菜中都存在第2类的SLG基因,而且,...通过不同甘蓝型油菜中SLG基因的克隆及序列分析,探索了甘蓝型油菜中是否存在与芸薹属二倍体中相同或相似的S-locus基因。对10个甘蓝型油菜品种和品系中SLG基因的PCR扩增和序列比较分析发现,这些甘蓝型油菜中都存在第2类的SLG基因,而且,同二倍体芸薹属物种一样,第2类SLG基因之间具有较高的同源性;只有5个甘蓝型油菜品种和品系中存在第一类SLG基因,而且这些基因序列之间表现出高度的保守性,即同源性在96%以上,明显高于不同等位基因之间的同源性。这些甘蓝型油菜中的class I SLG基因可能源于同一个自交不亲和单体。展开更多
The analysis of the coupling mechanism of thermal-hydraulic-mechanical(THM)fields,and solid-liquidgas(SLG)phases during gas extraction process is of profound significance to explore its numerical application in the ga...The analysis of the coupling mechanism of thermal-hydraulic-mechanical(THM)fields,and solid-liquidgas(SLG)phases during gas extraction process is of profound significance to explore its numerical application in the gas occurrence regularity and its effective extraction radius.In this study,the Hudi coal mine in Qinshui basin is taken as the research area,the influencing factors of gas occurrence were analyzed,the differences in overburden load for gas pressure distribution and the factors influencing the effective extraction radius were further discussed by using the COMSOL software.The results show that the derivation of mathematical model in gas extraction shows that the process is a process the THM fields restrict each other,and the SLG phases influence each other.The longer the extraction time,the larger the influencing range of borehole,and the better the extraction effect.The larger the diameter of borehole,the larger the effective extraction radius,and the influence on gas extraction effect is smaller in the early stage and larger in the late stage.The borehole arrangement should be flexibly arranged according to the actual extraction situation.The higher the porosity,the higher the permeability,the better the gas extraction effect.The larger the overburden load of reservoir,the stronger the effective stress,which will result in the more severe the strain,and the closure of pore and fracture,which in turn will lead to the decrease of permeability and slow down the gas extraction.The relationship among extraction time,borehole diameter,negative pressure of gas extraction,permeability with effective extraction radius is exponential.This study has important theoretical and practical significance for clarifying and summarizing the gas occurrence regularity and its engineering practice.展开更多
文摘通过不同甘蓝型油菜中SLG基因的克隆及序列分析,探索了甘蓝型油菜中是否存在与芸薹属二倍体中相同或相似的S-locus基因。对10个甘蓝型油菜品种和品系中SLG基因的PCR扩增和序列比较分析发现,这些甘蓝型油菜中都存在第2类的SLG基因,而且,同二倍体芸薹属物种一样,第2类SLG基因之间具有较高的同源性;只有5个甘蓝型油菜品种和品系中存在第一类SLG基因,而且这些基因序列之间表现出高度的保守性,即同源性在96%以上,明显高于不同等位基因之间的同源性。这些甘蓝型油菜中的class I SLG基因可能源于同一个自交不亲和单体。
基金financially supported by the University Synergy Innovation Program of Anhui Province(No.GXXT-2021-018)the National Natural Science Foundation of China(No.42102217)+3 种基金the Natural Science Research Project of Anhui University(Nos.KJ2020A0315,KJ2020A0317)the Institute of Energy,Hefei Comprehensive National Science Center(No.21KZS218)the Natural Science Foundation of Anhui Province(No.2108085MD134)the Foundation of State Key Laboratory of Petroleum Resources and Prospecting,China University of Petroleum,Beijing(No.PRP/open-2005)
文摘The analysis of the coupling mechanism of thermal-hydraulic-mechanical(THM)fields,and solid-liquidgas(SLG)phases during gas extraction process is of profound significance to explore its numerical application in the gas occurrence regularity and its effective extraction radius.In this study,the Hudi coal mine in Qinshui basin is taken as the research area,the influencing factors of gas occurrence were analyzed,the differences in overburden load for gas pressure distribution and the factors influencing the effective extraction radius were further discussed by using the COMSOL software.The results show that the derivation of mathematical model in gas extraction shows that the process is a process the THM fields restrict each other,and the SLG phases influence each other.The longer the extraction time,the larger the influencing range of borehole,and the better the extraction effect.The larger the diameter of borehole,the larger the effective extraction radius,and the influence on gas extraction effect is smaller in the early stage and larger in the late stage.The borehole arrangement should be flexibly arranged according to the actual extraction situation.The higher the porosity,the higher the permeability,the better the gas extraction effect.The larger the overburden load of reservoir,the stronger the effective stress,which will result in the more severe the strain,and the closure of pore and fracture,which in turn will lead to the decrease of permeability and slow down the gas extraction.The relationship among extraction time,borehole diameter,negative pressure of gas extraction,permeability with effective extraction radius is exponential.This study has important theoretical and practical significance for clarifying and summarizing the gas occurrence regularity and its engineering practice.
