Following dental implantation,the characteristic bacterial milieu of the oral cavity may lead to peri-implant inflammation,which can negatively impact osseointegration and cause implant failure.To improve soft tissue ...Following dental implantation,the characteristic bacterial milieu of the oral cavity may lead to peri-implant inflammation,which can negatively impact osseointegration and cause implant failure.To improve soft tissue sealing around the implant,enhance osseointegration,and improve implant success rates,this paper proposes a composite multifunctional coating(PHG)prepared using gelatin and polydopamine/hydroxyapatite nanoparticles,investigates the effects of this novel coating on cell adhesion,proliferation,antibacterial activity,osteogenic differentiation,and evaluates its immune-related properties.The PHG coating was proved to have satisfactory hydrophilicity and wettability for cell attachment.Furthermore,it improved the expression of adhesion-related genes and proteins in human gingival fibroblasts,indicating its adhesion-promoting effect.Additionally,bone marrow mesenchymal stem cells exhibited strong osteogenic differentiation potential and mineralization on PHG-coated surfaces.Notably,the PHG coating exhibited antibacterial activity against Streptococcus mutans,as well as anti-inflammatory effects,potentially via the regulation of macrophages.Therefore,the proposed PHG coating may promote soft tissue sealing and bone bonding,providing a potential strategy for the surface modification of dental implants.展开更多
Transcutaneous implants that penetrate through skin or mucosa are susceptible to bacteria invasion and lack proper soft tissue sealing.Traditional antibacterial strategies primarily focus on bacterial eradication,but ...Transcutaneous implants that penetrate through skin or mucosa are susceptible to bacteria invasion and lack proper soft tissue sealing.Traditional antibacterial strategies primarily focus on bacterial eradication,but excessive exposure to bactericidal agents can induce noticeable tissue damage.Herein,a rechargeable model(HPI-Ti)was constructed using perylene polyimide,an aqueous battery material,achieving temporal-sequence regulation of bacterial killing and soft tissue sealing.Charge storage within HPI-Ti is achieved after galvanostatic charge,and chemical discharge is initiated when immersed in physiological environments.During the early discharge stage,post-charging HPI-Ti demonstrates an antibacterial rate of 99.96±0.01%for 24 h,preventing biofilm formation.Contact-dependent violent electron transfer between bacteria and the material causes bacteria death.In the later discharge stage,the attenuated discharging status creates a gentler electron-transfer microenvironment for fibroblast proliferation.After discharge,the antibacterial activity can be reinstated by recharge against potential reinfection.The antibacterial efficacy and soft tissue compatibility were verified in vivo.These results demonstrate the potential of the charge-transfer-based model in reconciling antibacterial efficacy with tissue compatibility.展开更多
Soft tissue seal around the transmucosal region of dental implants is crucial for shielding oral bacterial invasion and guaranteeing the long-term functioning of implants.Compared with the robust periodontal tissue ba...Soft tissue seal around the transmucosal region of dental implants is crucial for shielding oral bacterial invasion and guaranteeing the long-term functioning of implants.Compared with the robust periodontal tissue barrier around a natural tooth,the peri-implant mucosa presents a lower bonding efficiency to the transmucosal region of dental implants,due to physiological structural differences.As such,the weaker soft tissue seal around the transmucosal region can be easily broken by oral pathogens,which may stimulate serious inflammatory responses and lead to the development of peri-implant mucositis.Without timely treatment,the curable peri-implant mucositis would evolve into irreversible peri-implantitis,finally causing the failure of implantation.Herein,this review has summarized current surface modification strategies for the transmucosal region of dental implants with improved soft tissue bonding capacities(e.g.,improving surface wettability,fabricating micro/nano topographies,altering the surface chemical composition and constructing bioactive coatings).Furthermore,the surfaces with advanced soft tissue bonding abilities can be incorporated with antibacterial properties to prevent infections,and/or with immunomodulatory designs to facilitate the establishment of soft tissue seal.Finally,we proposed future research orientations for developing multifunctional surfaces,thus establishing a firm soft tissue seal at the transmucosal region and achieving the long-term predictability of dental implants.展开更多
Peri-implant epithelial sealing is the first line of defense against external pathogens or stimuli;hence,an essential process to prevent peri-implantitis.Laminin 332(LN332)is the main component of the internal basal l...Peri-implant epithelial sealing is the first line of defense against external pathogens or stimuli;hence,an essential process to prevent peri-implantitis.Laminin 332(LN332)is the main component of the internal basal lamina and participates in peri-implant epithelial sealing by forming hemidesmosomes(HDs)with integrinα6β4.In thiswork,poly(D,L-lactide)(PDLLA)-LN332 composite coating was successfully constructed by a method similar to layer-by-layer assembly,displaying staged LN332 release for as long as 28days.The PDLLA-LN332 composite coating can activate the intracellular PI3K-Akt pathway via binding to cellular integrinα6β4,which can promote adhesion,migration and proliferation of HaCaT cells and further enhance the expression of keratinocyte HD-related molecules,including integrinα6β4,LN332 and plectin.Furthermore,the PDLLA-LN332 composite coating can promote the adhesion,spreading and proliferation of gingival mesenchymal stem cells and accelerate their epithelial differentiation.Therefore,the PDLLA-LN332 composite coating can enhance implant soft tissue sealing,warranting further in vivo study.展开更多
基金funded by the National Natural Science Foundation of China(Nos.81801006,31870953,81901048,81620108006,81991505,81921002,81801023,and 82100963)Shanghai Rising-Star Program(21QA1405400)+1 种基金the National Key Research and Development Program of China(No.2016YFC1102900)Innovative Research Team of High-Level Local Universities in Shanghai(No.SSMU-ZDCX20180900)。
文摘Following dental implantation,the characteristic bacterial milieu of the oral cavity may lead to peri-implant inflammation,which can negatively impact osseointegration and cause implant failure.To improve soft tissue sealing around the implant,enhance osseointegration,and improve implant success rates,this paper proposes a composite multifunctional coating(PHG)prepared using gelatin and polydopamine/hydroxyapatite nanoparticles,investigates the effects of this novel coating on cell adhesion,proliferation,antibacterial activity,osteogenic differentiation,and evaluates its immune-related properties.The PHG coating was proved to have satisfactory hydrophilicity and wettability for cell attachment.Furthermore,it improved the expression of adhesion-related genes and proteins in human gingival fibroblasts,indicating its adhesion-promoting effect.Additionally,bone marrow mesenchymal stem cells exhibited strong osteogenic differentiation potential and mineralization on PHG-coated surfaces.Notably,the PHG coating exhibited antibacterial activity against Streptococcus mutans,as well as anti-inflammatory effects,potentially via the regulation of macrophages.Therefore,the proposed PHG coating may promote soft tissue sealing and bone bonding,providing a potential strategy for the surface modification of dental implants.
基金supported by National Natural Science Foundation of China(52272283)Key Research and Development Program of Zhejiang Province(2023C03G9103957)+2 种基金Young Elite Scientists Sponsorship Program by CAST(YESS)(2022-2024QNRC001)Youth Innovation Promotion Association CAS(2023263)Major/Key Program of State Key Laboratory for Modification of Chemical Fibers and Polymer Materials(23M1060280).
文摘Transcutaneous implants that penetrate through skin or mucosa are susceptible to bacteria invasion and lack proper soft tissue sealing.Traditional antibacterial strategies primarily focus on bacterial eradication,but excessive exposure to bactericidal agents can induce noticeable tissue damage.Herein,a rechargeable model(HPI-Ti)was constructed using perylene polyimide,an aqueous battery material,achieving temporal-sequence regulation of bacterial killing and soft tissue sealing.Charge storage within HPI-Ti is achieved after galvanostatic charge,and chemical discharge is initiated when immersed in physiological environments.During the early discharge stage,post-charging HPI-Ti demonstrates an antibacterial rate of 99.96±0.01%for 24 h,preventing biofilm formation.Contact-dependent violent electron transfer between bacteria and the material causes bacteria death.In the later discharge stage,the attenuated discharging status creates a gentler electron-transfer microenvironment for fibroblast proliferation.After discharge,the antibacterial activity can be reinstated by recharge against potential reinfection.The antibacterial efficacy and soft tissue compatibility were verified in vivo.These results demonstrate the potential of the charge-transfer-based model in reconciling antibacterial efficacy with tissue compatibility.
基金supported by the National Key Research and Development Program of China(2023YFC2412600)the National Natural Science Foundation of China(52271243,52171233,82370924)+4 种基金the NSFC-RFBR Joint Research Scheme(82361138575)the Beijing Nova Program(20230484459)the Beijing Natural Science Foundation(7242173)the Clinical Medicine Plus X-Young Scholars Project of Peking Universitythe Fundamental Research Funds for the Central Universities(PKU2024LCXQ014).
文摘Soft tissue seal around the transmucosal region of dental implants is crucial for shielding oral bacterial invasion and guaranteeing the long-term functioning of implants.Compared with the robust periodontal tissue barrier around a natural tooth,the peri-implant mucosa presents a lower bonding efficiency to the transmucosal region of dental implants,due to physiological structural differences.As such,the weaker soft tissue seal around the transmucosal region can be easily broken by oral pathogens,which may stimulate serious inflammatory responses and lead to the development of peri-implant mucositis.Without timely treatment,the curable peri-implant mucositis would evolve into irreversible peri-implantitis,finally causing the failure of implantation.Herein,this review has summarized current surface modification strategies for the transmucosal region of dental implants with improved soft tissue bonding capacities(e.g.,improving surface wettability,fabricating micro/nano topographies,altering the surface chemical composition and constructing bioactive coatings).Furthermore,the surfaces with advanced soft tissue bonding abilities can be incorporated with antibacterial properties to prevent infections,and/or with immunomodulatory designs to facilitate the establishment of soft tissue seal.Finally,we proposed future research orientations for developing multifunctional surfaces,thus establishing a firm soft tissue seal at the transmucosal region and achieving the long-term predictability of dental implants.
基金supported by the National Natural Science Foundation of China(No.81970971)Shaanxi Key Research and Development Program(No.2022SF-179).
文摘Peri-implant epithelial sealing is the first line of defense against external pathogens or stimuli;hence,an essential process to prevent peri-implantitis.Laminin 332(LN332)is the main component of the internal basal lamina and participates in peri-implant epithelial sealing by forming hemidesmosomes(HDs)with integrinα6β4.In thiswork,poly(D,L-lactide)(PDLLA)-LN332 composite coating was successfully constructed by a method similar to layer-by-layer assembly,displaying staged LN332 release for as long as 28days.The PDLLA-LN332 composite coating can activate the intracellular PI3K-Akt pathway via binding to cellular integrinα6β4,which can promote adhesion,migration and proliferation of HaCaT cells and further enhance the expression of keratinocyte HD-related molecules,including integrinα6β4,LN332 and plectin.Furthermore,the PDLLA-LN332 composite coating can promote the adhesion,spreading and proliferation of gingival mesenchymal stem cells and accelerate their epithelial differentiation.Therefore,the PDLLA-LN332 composite coating can enhance implant soft tissue sealing,warranting further in vivo study.
