采用SMART(switching metchanism at 5’end of RNA transcript)技术构建缢蛏cDNA基因文库。并对cDNA文库的滴度,重组率和插入片段的大小进行了检测。结果表明,该cDNA文库的滴度为5.50×104cfu/ml,重组率为92.5%,插入片段的长度大多...采用SMART(switching metchanism at 5’end of RNA transcript)技术构建缢蛏cDNA基因文库。并对cDNA文库的滴度,重组率和插入片段的大小进行了检测。结果表明,该cDNA文库的滴度为5.50×104cfu/ml,重组率为92.5%,插入片段的长度大多在1kb以上。对478个克隆子进行了测序,共得到430个有效序列,其中166个序列无同源性,264条序列具有同源性。从中得到肌动蛋白(actin)cDNA的全长1538bp,开放阅读框(ORF)为1131bp,编码376个氨基酸,其预测蛋白的分子量为41.78kD,等电点为5.30。该氨基酸序列与其它物种的同源性大都在90%以上。展开更多
The genetic variation and differentiation of the razor clam Sinonovacula constricta distributed along the coast of China were studied through amplified fragment length polymorphism(AFLP) analysis.Six primer combinatio...The genetic variation and differentiation of the razor clam Sinonovacula constricta distributed along the coast of China were studied through amplified fragment length polymorphism(AFLP) analysis.Six primer combinations generated 193 fragments.The He values varied from 0.322 to 0.463 and the percentage of polymorphic loci ranged from 74.1% to 98.4%,which indicates a high level of genetic diversity.Cluster analysis by Nei's pairwise distance grouped all specimens by geographical origins.AMOVA consistently showed that genetic variation among populations was 8.71%,and most of the variation came from the genetic variation within populations(91.29%).Genetic differentiation among the six populations was moderate;pairwise FST ranged from 0.0282 to 0.1480,which indicated that S.constricta populations along the coast of China are genetically connected.Among all the six populations,the Beihai population is the mostly differentiated from the others,suggesting that Hainan Island and Leizhou Peninsula act as barriers to gene flow.All populations abide isolation by distance model as indicated by Mantel test,except for ZS(Zhoushan) and YQ(Yueqing) populations.Information obtained in this study will provide guidelines for conservation and fishery management of this species in the future.展开更多
The nucleotide sequence of the mitochondrial genome of the solecurtidae Bivalvia mollusca Sinonovacula constricata（GenBank accession number EU880278） has been determined and is reported here. We determined the compl...The nucleotide sequence of the mitochondrial genome of the solecurtidae Bivalvia mollusca Sinonovacula constricata（GenBank accession number EU880278） has been determined and is reported here. We determined the complete mitochondrial genome sequence using long-PCR and Shot Gun Sequencing. Contained within the 17 225 base pairs （bp） are the two ribosomal RNA genes and 12 protein coding genes typical of metazoan mitochondrial genomes. The S. constricta mitochondrial DNA （mtDNA） did not contain a gene for atp8, similar to the mtDNA of Crassostrea virginica, Crassostrea giga and Mytilus edulis. The S. constricta mtDNA is 67.0% A＋T （A 25.9%, C 10.5%, G 22.5%, and T 41.1%）. This value is higher than that for many invertebrate mitochondrial genomes. Only 19 putative tRNA genes are present in S. constricta and 27 noncoding regions, of which two are large in size. The trnE and trnW genes as well as a second trnS were absent in S. constricta. The gene arrangement of S. constricta is different from the other Bivalvia genomes.展开更多
文摘采用SMART(switching metchanism at 5’end of RNA transcript)技术构建缢蛏cDNA基因文库。并对cDNA文库的滴度,重组率和插入片段的大小进行了检测。结果表明,该cDNA文库的滴度为5.50×104cfu/ml,重组率为92.5%,插入片段的长度大多在1kb以上。对478个克隆子进行了测序,共得到430个有效序列,其中166个序列无同源性,264条序列具有同源性。从中得到肌动蛋白(actin)cDNA的全长1538bp,开放阅读框(ORF)为1131bp,编码376个氨基酸,其预测蛋白的分子量为41.78kD,等电点为5.30。该氨基酸序列与其它物种的同源性大都在90%以上。
基金supported by the grants from National High Technology Research and Development Program (2007AA09Z433)the Major State Basic Research Development Program of China (973 Program, 2010CB-126406)Chinese Ministry of Education (707041)
文摘The genetic variation and differentiation of the razor clam Sinonovacula constricta distributed along the coast of China were studied through amplified fragment length polymorphism(AFLP) analysis.Six primer combinations generated 193 fragments.The He values varied from 0.322 to 0.463 and the percentage of polymorphic loci ranged from 74.1% to 98.4%,which indicates a high level of genetic diversity.Cluster analysis by Nei's pairwise distance grouped all specimens by geographical origins.AMOVA consistently showed that genetic variation among populations was 8.71%,and most of the variation came from the genetic variation within populations(91.29%).Genetic differentiation among the six populations was moderate;pairwise FST ranged from 0.0282 to 0.1480,which indicated that S.constricta populations along the coast of China are genetically connected.Among all the six populations,the Beihai population is the mostly differentiated from the others,suggesting that Hainan Island and Leizhou Peninsula act as barriers to gene flow.All populations abide isolation by distance model as indicated by Mantel test,except for ZS(Zhoushan) and YQ(Yueqing) populations.Information obtained in this study will provide guidelines for conservation and fishery management of this species in the future.
基金"863" Hi-tech Research and Development Program of China under contract No. 2006 AA10 A410Shanghai Leading Academic Discipline Project under contract No. Y1101Dr. Start-up Fund under contract No. A-2400-09-0144
文摘The nucleotide sequence of the mitochondrial genome of the solecurtidae Bivalvia mollusca Sinonovacula constricata（GenBank accession number EU880278） has been determined and is reported here. We determined the complete mitochondrial genome sequence using long-PCR and Shot Gun Sequencing. Contained within the 17 225 base pairs （bp） are the two ribosomal RNA genes and 12 protein coding genes typical of metazoan mitochondrial genomes. The S. constricta mitochondrial DNA （mtDNA） did not contain a gene for atp8, similar to the mtDNA of Crassostrea virginica, Crassostrea giga and Mytilus edulis. The S. constricta mtDNA is 67.0% A＋T （A 25.9%, C 10.5%, G 22.5%, and T 41.1%）. This value is higher than that for many invertebrate mitochondrial genomes. Only 19 putative tRNA genes are present in S. constricta and 27 noncoding regions, of which two are large in size. The trnE and trnW genes as well as a second trnS were absent in S. constricta. The gene arrangement of S. constricta is different from the other Bivalvia genomes.
