BACKGROUND Transcrestal sinus floor elevation(TSFE)has been widely used in the oral clinic when the residual bone height(RBH)exceeds 5 mm.However,when there is insufficient RBH in the posterior maxilla,two-stage TSFE ...BACKGROUND Transcrestal sinus floor elevation(TSFE)has been widely used in the oral clinic when the residual bone height(RBH)exceeds 5 mm.However,when there is insufficient RBH in the posterior maxilla,two-stage TSFE may be an option.CASE SUMMARY This article introduces the concept of two-stage TSFE.Six patients had osseointegration failure after TSFE.For the first-stage surgery,we restricted the vertical bone augmentation as much as possible.At the second-stage surgery,the increased RBH was 3.28±1.55 mm,which was beneficial for surgery.Five implants functioned successfully on schedule,but one implant failed again during the healing period.A third surgery was performed,and the implant functioned successfully.CONCLUSION When RBH was less than 5 mm,two or more procedures of TSFE might result in a higher RBH.展开更多
The aim of the present study was to investigate an inorganic bovine-derived hydroxyapatite bone substitute (Osteograph?) mixed with the same biomaterial coated with a synthetic peptide (P-15) analogue of collagen (Pep...The aim of the present study was to investigate an inorganic bovine-derived hydroxyapatite bone substitute (Osteograph?) mixed with the same biomaterial coated with a synthetic peptide (P-15) analogue of collagen (PepGen P-15?). This blend of bone replacement materials was used for sinus floor augmentation. Assessments were carried out by using histology methods, transmission electron microscopy (TEM) and microanalysis (EDX). Ultrastructural and analytical features of the interfaces between the graft material and the peri-biomaterial tissues were evaluated six months after implantation. Our findings clearly show that newly-formed crystallites first develop at the surface of implanted crystals. Histological investigations revealed new bone tissue linking biomaterial particles together. TEM assessments pointed out that lamellar bone was generally separated from the graft material by a layer of woven bone measuring between 1 and 1.5 μm in thickness. Although calcified bone tissue was observed in direct contact with bone filling particles, the presence of mineralized granular material around implanted particles was also noticed. No characteristic periodic striation of mineralized collagen was evident within that mineralized structure. Chemical analyses (TEM-EDX) realized at different locations of newly formed mineralized granular substance along the interface revealed average Ca/P ratios ranging between 1.02 and 1.63. The different, concomitantly occurring, aforementioned structural features of the interfaces strongly suggested that the host responses to the used biomaterial blend resulted from dynamic osseointegration phenomena related to various interfacial mechanisms. Nevertheless, the biological response to the bone graft material appeared clinically and histologically satisfactory.展开更多
BACKGROUND In this case study,a minimally invasive transalveolar approach using platelet-rich fibrin and bone substitute with simultaneous implantation was carried out in an elderly patient.We analyzed the cone-beam c...BACKGROUND In this case study,a minimally invasive transalveolar approach using platelet-rich fibrin and bone substitute with simultaneous implantation was carried out in an elderly patient.We analyzed the cone-beam computed tomography(CBCT)findings to evaluate bone regeneration.CASE SUMMARY A 65-year-old female with no contraindications for dental implants and loss of maxillary bilateral molars is described.Examination by CBCT showed the available vertical bone height in the bilateral posterior maxilla was 0.5-6.8 mm in the left and 2.8-6.5 mm in the right.The patient underwent a transalveolar approach using platelet-rich fibrin and bone substitute with simulataneous placement of an implant 10 mm in length.Six months post-surgery,the implant showed excellent osseointegration with the bone graft.Thereafter,full-ceramic crowns were fitted.Follow-up at 2 years demonstrated satisfactory prognosis.CONCLUSION Platelet-rich fibrin and bone substitute can be used to augment the maxillary sinus with a vertical bone height less than 4 mm.展开更多
Introduction: Platelet Rich-Fibrin (PRF) is a biological matrix derived from a patient’s own blood, rich in growth factors and platelets. Its use in various periodontal and non-periodontal procedures is gaining recog...Introduction: Platelet Rich-Fibrin (PRF) is a biological matrix derived from a patient’s own blood, rich in growth factors and platelets. Its use in various periodontal and non-periodontal procedures is gaining recognition due to its potential in promoting tissue regeneration. The purpose of this review was to evaluate the benefits of using PRF in intra-bony defect regeneration, guided-bone regeneration, and sinus floor elevation. Methods: The study searched PubMed for manuscripts published between 2017 and 2022 to better understand the clinical and radiological effects of PRF. The manuscripts were divided into the following sections: intra-bony defect regeneration, guided-bone regeneration, and sinus floor elevation. Results: In intra-bony defects, PRF improved clinical and radiological parameters when compared with OFD alone, with a significant difference in wound healing at 7 days. In GBR, a CBCT evaluation shows no statistical difference between the PRF-autogenous bone complex group and the bovine bone-collagen membrane complex regarding volume change of the augmented bone with a 16% rate of bone loss following a 6-month healing period. Also, a slight increase in bone thickness has been seen when liquid PRF is used. In sinus floor elevation, results revealed no differences in graft volume between PRF group and control group at any of the evaluated time points. Although higher implant stability immediately postoperatively, higher new bone formation, the lesser amount of residual graft and earlier implant placement. Conclusion: Platelet Rich-Fibrin is widely accepted for use in periodontal surgery and dentistry due to its minimally invasive nature and low risk of adverse effects, with positive results in tissue regeneration. There is evidence that PRF leads to improved and faster healing, as well as cost-effective regenerative procedures compared to other treatments.展开更多
The aim of the study was to analyze the histologic and ultrastructural changes after maxillary sinus augmentation with simultaneous implant placement using engineered bone graft material.In this study,calcium phosphat...The aim of the study was to analyze the histologic and ultrastructural changes after maxillary sinus augmentation with simultaneous implant placement using engineered bone graft material.In this study,calcium phosphate cement(CPC)scaffolds combined with goat bone marrow stromal cells(BMSCs)were used to fill goat sinus floor space after maxillary sinus floor elevation with simultaneous implant placement comparing with those not filled any grafted materials and used as controls.After a healing period of 3 months,the goat maxillary sinus membrane was examined using light microscopy and scanning electronic microscopy.The results showed that the connective tissue thickness and the epithelium thickness of mucosa were not statistically significant difference between two groups.The tissue engineered bone complex might be an ideal graft for the sinus floor elevation and have no influence on the sinus membrane under the histological and ultrastructural observation.展开更多
基金the Wenzhou Science and Technology Bureau Projects,No.Y20190105.
文摘BACKGROUND Transcrestal sinus floor elevation(TSFE)has been widely used in the oral clinic when the residual bone height(RBH)exceeds 5 mm.However,when there is insufficient RBH in the posterior maxilla,two-stage TSFE may be an option.CASE SUMMARY This article introduces the concept of two-stage TSFE.Six patients had osseointegration failure after TSFE.For the first-stage surgery,we restricted the vertical bone augmentation as much as possible.At the second-stage surgery,the increased RBH was 3.28±1.55 mm,which was beneficial for surgery.Five implants functioned successfully on schedule,but one implant failed again during the healing period.A third surgery was performed,and the implant functioned successfully.CONCLUSION When RBH was less than 5 mm,two or more procedures of TSFE might result in a higher RBH.
文摘The aim of the present study was to investigate an inorganic bovine-derived hydroxyapatite bone substitute (Osteograph?) mixed with the same biomaterial coated with a synthetic peptide (P-15) analogue of collagen (PepGen P-15?). This blend of bone replacement materials was used for sinus floor augmentation. Assessments were carried out by using histology methods, transmission electron microscopy (TEM) and microanalysis (EDX). Ultrastructural and analytical features of the interfaces between the graft material and the peri-biomaterial tissues were evaluated six months after implantation. Our findings clearly show that newly-formed crystallites first develop at the surface of implanted crystals. Histological investigations revealed new bone tissue linking biomaterial particles together. TEM assessments pointed out that lamellar bone was generally separated from the graft material by a layer of woven bone measuring between 1 and 1.5 μm in thickness. Although calcified bone tissue was observed in direct contact with bone filling particles, the presence of mineralized granular material around implanted particles was also noticed. No characteristic periodic striation of mineralized collagen was evident within that mineralized structure. Chemical analyses (TEM-EDX) realized at different locations of newly formed mineralized granular substance along the interface revealed average Ca/P ratios ranging between 1.02 and 1.63. The different, concomitantly occurring, aforementioned structural features of the interfaces strongly suggested that the host responses to the used biomaterial blend resulted from dynamic osseointegration phenomena related to various interfacial mechanisms. Nevertheless, the biological response to the bone graft material appeared clinically and histologically satisfactory.
基金Supported by the Jilin Province Science and Technology Development Plan Project,No.20180101123JC13th Five-Year Science and Technology Project of Jilin Provincial Education Department,No.JJKH20190096KJ+2 种基金Jilin Province Health and Health Technology Innovation Project,No.2018J072Project of Jilin Provincial Development and Reform Commission,No.2019C051-2and Jilin Province TCM Science and Technology Project,No.2019036。
文摘BACKGROUND In this case study,a minimally invasive transalveolar approach using platelet-rich fibrin and bone substitute with simultaneous implantation was carried out in an elderly patient.We analyzed the cone-beam computed tomography(CBCT)findings to evaluate bone regeneration.CASE SUMMARY A 65-year-old female with no contraindications for dental implants and loss of maxillary bilateral molars is described.Examination by CBCT showed the available vertical bone height in the bilateral posterior maxilla was 0.5-6.8 mm in the left and 2.8-6.5 mm in the right.The patient underwent a transalveolar approach using platelet-rich fibrin and bone substitute with simulataneous placement of an implant 10 mm in length.Six months post-surgery,the implant showed excellent osseointegration with the bone graft.Thereafter,full-ceramic crowns were fitted.Follow-up at 2 years demonstrated satisfactory prognosis.CONCLUSION Platelet-rich fibrin and bone substitute can be used to augment the maxillary sinus with a vertical bone height less than 4 mm.
文摘Introduction: Platelet Rich-Fibrin (PRF) is a biological matrix derived from a patient’s own blood, rich in growth factors and platelets. Its use in various periodontal and non-periodontal procedures is gaining recognition due to its potential in promoting tissue regeneration. The purpose of this review was to evaluate the benefits of using PRF in intra-bony defect regeneration, guided-bone regeneration, and sinus floor elevation. Methods: The study searched PubMed for manuscripts published between 2017 and 2022 to better understand the clinical and radiological effects of PRF. The manuscripts were divided into the following sections: intra-bony defect regeneration, guided-bone regeneration, and sinus floor elevation. Results: In intra-bony defects, PRF improved clinical and radiological parameters when compared with OFD alone, with a significant difference in wound healing at 7 days. In GBR, a CBCT evaluation shows no statistical difference between the PRF-autogenous bone complex group and the bovine bone-collagen membrane complex regarding volume change of the augmented bone with a 16% rate of bone loss following a 6-month healing period. Also, a slight increase in bone thickness has been seen when liquid PRF is used. In sinus floor elevation, results revealed no differences in graft volume between PRF group and control group at any of the evaluated time points. Although higher implant stability immediately postoperatively, higher new bone formation, the lesser amount of residual graft and earlier implant placement. Conclusion: Platelet Rich-Fibrin is widely accepted for use in periodontal surgery and dentistry due to its minimally invasive nature and low risk of adverse effects, with positive results in tissue regeneration. There is evidence that PRF leads to improved and faster healing, as well as cost-effective regenerative procedures compared to other treatments.
基金the Natural Science Foundation of Science and Technology Commission of Shanghai Municipality (Nos.09JC1411700 and S30206)the Natural Science Foundation of Shanghai Jiaotong University School of Medicine(No.09XJ21030)
文摘The aim of the study was to analyze the histologic and ultrastructural changes after maxillary sinus augmentation with simultaneous implant placement using engineered bone graft material.In this study,calcium phosphate cement(CPC)scaffolds combined with goat bone marrow stromal cells(BMSCs)were used to fill goat sinus floor space after maxillary sinus floor elevation with simultaneous implant placement comparing with those not filled any grafted materials and used as controls.After a healing period of 3 months,the goat maxillary sinus membrane was examined using light microscopy and scanning electronic microscopy.The results showed that the connective tissue thickness and the epithelium thickness of mucosa were not statistically significant difference between two groups.The tissue engineered bone complex might be an ideal graft for the sinus floor elevation and have no influence on the sinus membrane under the histological and ultrastructural observation.
