Safety Observation Study on Haemophilus Influenza Type B Conjugate Vaccines Injected at Different Sites in Chinese Infants

In the present study, the safety of Hoemophilus influenza type b conjugate vaccines inoculated in the upper arm deltoid and vastus lateralis muscle was evaluated. 680 infants aged 2-5 months and 6-12 months were selected to be the research subjects in whom the Hib conjugate vaccines were inoculated by injection in the upper arm deltoid and vastus lateralis muscle, respectively. The safety analysis indicated that there were no statistic differences in the incidence rates of adverse reactions when the Hib conjugate vaccines were inoculated at different sites. So we concluded that the safety of inoculation injection of Hib conjugate vaccines in vastus lateralis muscle was the same as that inoculated in the upper arm deltoid.

Differences of carbon and nitrogen stoichiometry between different habitats in two natural Korean pine forests in Northeast China's mountainous areas

There is a strong link between vegetation and environmental factors.Therefore,to elucidate how the stoichiometric characteristics of plants respond to different habitats,we studied organic carbon(C)and total nitrogen(N)stoichiometry characteristics of plants in four different habitats of two different natural Korean pine forests.Three of the habitats(gaps,mounds,and pits)were formed via tree uprooting,whereas an undisturbed site(closed canopies)was used as a control.The followings were our main findings:(1)the organic carbon content of more than half of the 12 picked plants showed significant differences in different habitats.Most of the plants in pit-mound complexes exhibited lower organic carbon contents than plants in gaps and closed canopies;(2)there was no significant differences in the total nitrogen content in 13 of the 25 groups of plant samples;(3)interestingly,the C/N of the sample groups with significant differences in total nitrogen also showed significant differences;(4)there was no significant variations in the stoichiometric characteristics of plants that grew in gaps;however,the variations among plants that grew in pits and mounds were significant;(5)through redundancy analysis(RDA),we find that the main factors effecting the C and N stoichiometric characters of the plants were plants own regulation and the change of photosynthetically active radiation(PAR).In conclusion,the C and N stoichiometric characteristics of plants did not respond to gap formations,but they did strongly respond to pit-mound complex formations.Photosynthetically active radiation was the most important effective factor to plants in the four habitats.

Project of “Three Networks Greening” based on optimal allocation in the Yellow River Delta,China (Dongying section)

We have used the Yellow River Delta （Dongying section） as our study area to address the project of ＂Three Networks Greening＂ （TNG）. With the use of GIS technology and from an ecological point of view, an optimal allocation scheme of land resources is constructed and applied to guide the adjustment of land resources. Given this scheme, we have calculated that the area of land suitable for forest and shrubs without greening is 2256 km^2. Simultaneously, acting on the layout of the TNG project, afforestation site types are prepared and improved. Soil types, microrelief, salinity and underwater levels are combined as major classification factors and irrigation conditions as a reference to classify sites into eight types. In this way, land suitable for forest and grass is afforested given particular planting patterns. Finally, by overlaying this forestry site type map with the TNG plan map, some suggestions and strategies are proposed and used to direct the TNG project. An ecological oasis of the Yellow River Delta should be the result.

Critical role of Dengue Virus NS1 protein in viral replication

Dengue virus(DENV) nonstructural protein 1(NS1) is a highly conserved 46-kDa protein that contains 2 glycosylation sites(Asn-130 and Asn-207) and 12 conserved cysteine(Cys) residues. Here, we performed site-directed mutagenesis to generate systematic mutants of viral strain TSV01. The results of the subsequent analysis showed that an alanine substitution at the second N-linked glycan Asn-207 in NS1 delayed viral RNA synthesis, reduced virus plaque size, and weakened the cytopathic effect. Three mutants at Cys sites(Cys-4, Cys-55, Cys-291) and a C-terminal deletion(ΔC) mutant significantly impaired RNA synthesis, and consequently abolished viral growth, whereas alanine mutations at Asn-130 and Glu-173 resulted in phenotypes that were similar to the wild-type(WT) virus. Further analysis showed that the Asn-207 mutation slightly delayed viral replication. These results suggest that the three conserved disulfide bonds and the second N-linked glycan in NS1 are required for DENV-2 replication.

Functions and Plant Configuration of Wuhan Jiangtan Park

With the rapid development of social economy, and constant improvement of living standards, modern people have had higher requirements on neighboring environment. Park green space is an important component of urban ecosystem, it improves quality of urban ecological environment, reduces urban heat island effect, and beautifies city environment. By studying plant configuration in Jiangtan Park, Hankou District, Wuhan City, functional areas were regarded as a whole to collect data, AHP was applied to investigate the sample plots, garden plant landscape evaluation system was established, and landscapes in all units were evaluated. From the perspectives of site type, functional area, plant configuration, landscape composition, this paper analyzed site conditions of Jiangtan Park, provided scientific theoretic support and case reference for the plant configuration of park green spaces, which would play an extremely important role in improving urban ecological environment and enhancing urban landscape effect.