Multiple Tin Compounds Modified Carbon Fibers to Construct Heterogeneous Interfaces for Corrosion Prevention and Electromagnetic Wave Absorption

Currently,the demand for electromagnetic wave(EMW)absorbing materials with specific functions and capable of withstanding harsh environments is becoming increasingly urgent.Multi-component interface engineering is considered an effective means to achieve high-efficiency EMW absorption.However,interface modulation engineering has not been fully discussed and has great potential in the field of EMW absorption.In this study,multi-component tin compound fiber composites based on carbon fiber(CF)substrate were prepared by electrospinning,hydrothermal synthesis,and high-temperature thermal reduction.By utilizing the different properties of different substances,rich heterogeneous interfaces are constructed.This effectively promotes charge transfer and enhances interfacial polarization and conduction loss.The prepared SnS/SnS_(2)/SnO_(2)/CF composites with abundant heterogeneous interfaces have and exhibit excellent EMW absorption properties at a loading of 50 wt%in epoxy resin.The minimum reflection loss(RL)is−46.74 dB and the maximum effective absorption bandwidth is 5.28 GHz.Moreover,SnS/SnS_(2)/SnO_(2)/CF epoxy composite coatings exhibited long-term corrosion resistance on Q235 steel surfaces.Therefore,this study provides an effective strategy for the design of high-efficiency EMW absorbing materials in complex and harsh environments.

Defects‑Rich Heterostructures Trigger Strong Polarization Coupling in Sulfides/Carbon Composites with Robust Electromagnetic Wave Absorption

Defects-rich heterointerfaces integrated with adjustable crystalline phases and atom vacancies,as well as veiled dielectric-responsive character,are instrumental in electromagnetic dissipation.Conventional methods,however,constrain their delicate constructions.Herein,an innovative alternative is proposed:carrageenan-assistant cations-regulated(CACR)strategy,which induces a series of sulfides nanoparticles rooted in situ on the surface of carbon matrix.This unique configuration originates from strategic vacancy formation energy of sulfides and strong sulfides-carbon support interaction,benefiting the delicate construction of defects-rich heterostructures in M_(x)S_(y)/carbon composites(M-CAs).Impressively,these generated sulfur vacancies are firstly found to strengthen electron accumulation/consumption ability at heterointerfaces and,simultaneously,induct local asymmetry of electronic structure to evoke large dipole moment,ultimately leading to polarization coupling,i.e.,defect-type interfacial polarization.Such“Janus effect”(Janus effect means versatility,as in the Greek two-headed Janus)of interfacial sulfur vacancies is intuitively confirmed by both theoretical and experimental investigations for the first time.Consequently,the sulfur vacancies-rich heterostructured Co/Ni-CAs displays broad absorption bandwidth of 6.76 GHz at only 1.8 mm,compared to sulfur vacancies-free CAs without any dielectric response.Harnessing defects-rich heterostructures,this one-pot CACR strategy may steer the design and development of advanced nanomaterials,boosting functionality across diverse application domains beyond electromagnetic response.

Integration of Electrical Properties and Polarization Loss Modulation on Atomic Fe–N‑RGO for Boosting Electromagnetic Wave Absorption

Developing effective strategies to regulate graphene’s conduction loss and polarization has become a key to expanding its application in the electromagnetic wave absorption(EMWA)field.Based on the unique energy band structure of graphene,regulating its bandgap and electrical properties by introducing heteroatoms is considered a feasible solution.Herein,metal-nitrogen doping reduced graphene oxide(M–N-RGO)was prepared by embedding a series of single metal atoms M–N_(4) sites(M=Mn,Fe,Co,Ni,Cu,Zn,Nb,Cd,and Sn)in RGO using an N-coordination atom-assisted strategy.These composites had adjustable conductivity and polarization to optimize dielectric loss and impedance matching for efficient EMWA performance.The results showed that the minimum reflection loss(RL_(min))of Fe–N-RGO reaches−74.05 dB(2.0 mm)and the maximum effective absorption bandwidth(EAB_(max))is 7.05 GHz(1.89 mm)even with a low filler loading of only 1 wt%.Combined with X-ray absorption spectra(XAFS),atomic force microscopy,and density functional theory calculation analysis,the Fe–N_(4) can be used as the polarization center to increase dipole polarization,interface polarization and defect-induced polarization due to d-p orbital hybridization and structural distortion.Moreover,electron migration within the Fe further leads to conduction loss,thereby synergistically promoting energy attenuation.This study demonstrates the effectiveness of metal-nitrogen doping in regulating the graphene′s dielectric properties,which provides an important basis for further investigation of the loss mechanism.

Low‑Temperature Oxidation Induced Phase Evolution with Gradient Magnetic Heterointerfaces for Superior Electromagnetic Wave Absorption

Gradient magnetic heterointerfaces have injected infinite vitality in optimizing impedance matching,adjusting dielectric/magnetic resonance and promoting electromagnetic(EM)wave absorption,but still exist a significant challenging in regulating local phase evolution.Herein,accordion-shaped Co/Co_(3)O_(4)@N-doped carbon nanosheets(Co/Co_(3)O_(4)@NC)with gradient magnetic heterointerfaces have been fabricated via the cooperative high-temperature carbonization and lowtemperature oxidation process.The results indicate that the surface epitaxial growth of crystal Co_(3)O_(4) domains on local Co nanoparticles realizes the adjustment of magnetic-heteroatomic components,which are beneficial for optimizing impedance matching and interfacial polarization.Moreover,gradient magnetic heterointerfaces simultaneously realize magnetic coupling,and long-range magnetic diffraction.Specifically,the synthesized Co/Co_(3)O_(4)@NC absorbents display the strong electromagnetic wave attenuation capability of−53.5 dB at a thickness of 3.0 mm with an effective absorption bandwidth of 5.36 GHz,both are superior to those of single magnetic domains embedded in carbon matrix.This design concept provides us an inspiration in optimizing interfacial polarization,regulating magnetic coupling and promoting electromagnetic wave absorption.

Effects of mesoscale gravity waves on sporadic E simulated by a one-dimensional dynamic model

In addition to being driven by tidal winds,the sporadic E(Es)layers are modulated by gravity waves(GWs),although the effects are not yet comprehensively understood.In this article,we discuss the effects of mesoscale GWs on the Es layers determined by using a newly developed model,MISE-1D(one-dimensional Model of Ionospheric Sporadic E),with low numerical dissipation and high resolution.Driven by the wind fields resolved by the high-resolution version of the Whole Atmosphere Community Climate Model with thermosphere and ionosphere extension(WACCM-X),the MISE-1D simulation revealed that GWs significantly influence the evolution of the Es layer above 100 km but have a very limited effect at lower altitudes.The effects of GWs are diverse and complex,generally including the generation of fluctuating wavelike structures on the Es layer with frequencies similar to those of the GWs.The mesoscale GWs can also cause increases in the density of Es layers,or they can disperse or diffuse the Es layers and increase their thickness.In addition,the presence of GWs is a key factor in sustaining the Es layers in some cases.

Statistical analysis on the validity of the cold plasma approximation for chorus waves based on Van Allen Probe observations and their effects on radiation belt electrons

Theoretical analysis has demonstrated that the dispersion relation of chorus waves plays an essential role in the resonant interaction and energy transformation between the waves and magnetospheric electrons.Previous quantitative analyses often simplified the chorus dispersion relation by using the cold plasma assumption.However,the applicability of the cold plasma assumption is doubtful,especially during geomagnetic disturbances.We here present a systematic statistical analysis on the validity of the cold plasma dispersion relation of chorus waves based on observations from the Van Allen Probes over the period from 2012 to 2018.The statistical results show that the observed magnetic field intensities deviate substantially from those calculated from the cold plasma dispersion relation and that they become more pronounced with an increase in geomagnetic activity or a decrease in background plasma density.The region with large deviations is mainly concentrated in the nightside and expands in both the radial and azimuthal directions as the geomagnetic activity increases or the background plasma density decreases.In addition,the bounce-averaged electron scattering rates are computed by using the observed and cold plasma dispersion relation of chorus waves.Compared with usage of the cold plasma dispersion relation,usage of the observed dispersion relation considerably lowers the minimum resonant energy of electrons and lowers the scattering rates of electrons above tens of kiloelectronvolts but enhances those below.Furthermore,these differences are more pronounced with the enhancement of geomagnetic activity or the decrease in background plasma density.

Graphene Aerogel Composites with Self‑Organized Nanowires‑Packed Honeycomb Structure for Highly Efficient Electromagnetic Wave Absorption

With vigorous developments in nanotechnology,the elaborate regulation of microstructure shows attractive potential in the design of electromagnetic wave absorbers.Herein,a hierarchical porous structure and composite heterogeneous interface are constructed successfully to optimize the electromagnetic loss capacity.The macro–micro-synergistic graphene aerogel formed by the ice template‑assisted 3D printing strategy is cut by silicon carbide nanowires(SiC_(nws))grown in situ,while boron nitride(BN)interfacial structure is introduced on graphene nanoplates.The unique composite structure forces multiple scattering of incident EMWs,ensuring the combined effects of interfacial polarization,conduction networks,and magnetic-dielectric synergy.Therefore,the as-prepared composites present a minimum reflection loss value of−37.8 dB and a wide effective absorption bandwidth(EAB)of 9.2 GHz(from 8.8 to 18.0 GHz)at 2.5 mm.Besides,relying on the intrinsic high-temperature resistance of SiC_(nws) and BN,the EAB also remains above 5.0 GHz after annealing in air environment at 600℃ for 10 h.

An investigation on the wind profiles and gravity wave dynamics in MLT region based on the meteor radars from the Meridian Project

The meteor radar can detect the zenith angle,azimuth,radial velocity,and altitude of meteor trails so that one can invert the wind profiles in the mesosphere and low thermosphere(MLT)region,based on the Interferometric and Doppler techniques.In this paper,the horizontal wind field,gravity wave(GW)disturbance variance,and GW fluxes are analyzed through the meteor radar observation from 2012−2022,at Mohe(53.5°N,122.4°E)and Zuoling(30.5°N,114.6°E)stations of the(Chinese)Meridian Project.The Lomb−Scargle periodogram method has been utilized to analyze the periodic variations for time series with observational data gaps.The results show that the zonal winds at both stations are eastward dominated,while the meridional winds are southward dominated.The variance of GW disturbances in the zonal and meridional directions increases gradually with height,and there is a strong pattern of annual variation.The zonal momentum flux of GW changes little with height,showing weak annual variation.The meridional GW flux varies gradually from northward to southward with height,and the annual periodicity is stronger.For both stations,the maximum values of zonal and meridional wind occur close to the peak heights of GW flux,with opposite directions.This observational evidence is consistent with the filtering theory.The horizontal wind velocity,GW flux,and disturbance variance of the GW at Mohe are overall smaller than those at Zuoling,indicating weaker activities in the MLT at Mohe.The power spectral density(PSD)calculated by the Lomb−Scargle periodogram shows that there are 12-month period and 6-month period in horizontal wind field,GW disturbance variance and GW flux at both stations,and especially there is also a 4-month cycle in the disturbance variance.The PSD of the 12-month and 6-month cycles exhibits maximum values below 88 km and above 94 km.

Experimental and numerical simulation of the attenuation effect of blast shock waves in tunnels at different altitudes

Traffic engineering such as tunnels in various altitudinal gradient zone are at risk of accidental explosion,which can damage personnel and equipment.Accurate prediction of the distribution pattern of explosive loads and shock wave propagation process in semi-enclosed structures at various altitude environment is key research focus in the fields of explosion shock and fluid dynamics.The effect of altitude on the propagation of shock waves in tunnels was investigated by conducting explosion test and numerical simulation.Based on the experimental and numerical simulation results,a prediction model for the attenuation of the peak overpressure of tunnel shock waves at different altitudes was established.The results showed that the peak overpressure decreased at the same measurement points in the tunnel entrance under the high altitude condition.In contrast,an increase in altitude accelerated the propagation speed of the shock wave in the tunnel.The average error between the peak shock wave overpressure obtained using the overpressure prediction formula and the measured test data was less than15%,the average error between the propagation velocity of shock waves predicted values and the test data is less than 10%.The method can effectively predict the overpressure attenuation of blast wave in tunnel at various altitudes.

AI-based Correction of Wave Forecasts Using the Transformer-enhanced UNet Model

Grid forecasting can be used to effectively enhance the spatial and temporal density of forecast products,thereby improving the capability of short-term marine disaster forecasting and warnings in terms of proximity.The traditional method that relies on forecasters'subjective correction of station observation data for forecasting has been unable to meet the practical needs of refined forecasting.To address this problem,this paper proposes a Transformer-enhanced UNet(TransUNet)model for wave forecast AI correction,which fuses wind and wave information.The Transformer structure is integrated into the encoder of the UNet model,and instead of using the traditional upsampling method,the dual-sampling module is employed in the decoder to enhance the feature extraction capability.This paper compares the TransUNet model with the traditional UNet model using wind speed forecast data,wave height forecast data,and significant wave height reanalysis data provided by ECMWF.The experimental results indicate that the TransUNet model yields smaller root-meansquare errors,mean errors,and standard deviations of the corrected results for the next 24-h forecasts than does the UNet model.Specifically,the root-mean-square error decreased by more than 21.55%compared to its precorrection value.According to the statistical analysis,87.81%of the corrected wave height errors for the next 24-h forecast were within±0.2m,with only 4.56%falling beyond±0.3 m.This model effectively limits the error range and enhances the ability to forecast wave heights.

Role of variation coefficient of stone density in determining success of shock wave lithotripsy in urinary calculi

BACKGROUND Various stone factors can affect the net results of shock wave lithotripsy(SWL).Recently a new factor called variation coefficient of stone density(VCSD)is being considered to have an impact on stone free rates.AIM To assess the role of VCSD in determining success of SWL in urinary calculi.METHODS Charts review was utilized for collection of data variables.The patients were subjected to SWL,using an electromagnetic lithotripter.Mean stone density(MSD),stone heterogeneity index(SHI),and VCSD were calculated by generating regions of interest on computed tomography(CT)images.Role of these factors were determined by applying the relevant statistical tests for continuous and categorical variables and a P value of<0.05 was gauged to be statistically significant.RESULTS There were a total of 407 patients included in the analysis.The mean age of the subjects in this study was 38.89±14.61 years.In total,165 out of the 407 patients could not achieve stone free status.The successful group had a significantly lower stone volume as compared to the unsuccessful group(P<0.0001).Skin to stone distance was not dissimilar among the two groups(P=0.47).MSD was significantly lower in the successful group(P<0.0001).SHI and VCSD were both significantly higher in the successful group(P<0.0001).CONCLUSION VCSD,a useful CT based parameter,can be utilized to gauge stone fragility and hence the prediction of SWL outcomes.

A weak formulation of heterogenous viscoacoustic wave propagation in infinite domain

The accurate simulation of wave propagation in real media requires properly taking the attenuation into account,which leads to wave dissipation together with its causal companion,wave dispersion.In this study,to obtain a weak formulation of heterogenous viscoacoustic wave propagation in an infinite domain,the viscoacoustic medium is first characterized by its frequency-dependent complex bulk compliance instead of the classically used complex bulk modulus.Then,a mechanical model using serially connected standard linear solids(SSLS)is built to obtain the rational approximation of the complex bulk compliance whose parameters are calculated via an adapted nonlinear optimization method.Utilizing the obtained bulk compliance-based constitutive relation,a novel second-order viscoacoustic wave equation in the frequency domain is derived,of which the weak formulation can be physically explained as the virtual work equation and can thus be discretized using a continuous spectral element method in space.Additionally,a new method is introduced to address the convolution terms involved in the inverse Fourier transform,whose accurate time integration can then be achieved using an explicit time scheme,which avoids the transient growth that exists in the classical method.The resulting full time-space decoupling scheme can handle wave propagation in arbitrary heterogeneous media.Moreover,to treat the wave propagation in an infinite domain,a perfectly matched layer in weak formulation is derived for the truncation of the infinite domain via complex coordinate stretching of the virtual work equation.With only minor modification,the resulting perfectly matched layer can be implemented using the same time scheme as for the wave equation inside the truncated domain.The accuracy,numerical stability,and versatility of the new proposed scheme are demonstrated with numerical examples.

WavewatchⅢ模拟和统计方法在最大波高预报方面的评测分析

为了研究WavewatchⅢ(WWⅢ)海浪模型对最大波高的模拟能力及其与传统统计关系方法的差异,通过对两次台风浪过程的后报模拟和半年的业务化预报,分析了WWⅢ数值模拟的准确度及其与统计关系方法的精度差异。研究结果表明:WWⅢ数值模拟的最大波高(Hmax)的精度略低于有效波高(Hs),但也达到了24 h预报相对误差(H_(max)≥1 m)低于18%、相关系数高于0.94的水平,模拟精度可靠,可以用于业务化预报;与两种统计关系方法(H_(max)和H_(s)分别为1.42和1.52)计算的最大波高相比,数值模拟的精度总体与其相当,但在H_(max)和H_(s)比值大于1.65这种易出现危险的海况下,数值模拟具有更高的准确性,更适合应用于海浪预警报服务。

基于D-Wave Advantage的量子退火公钥密码攻击算法研究

D-Wave专用量子计算机的原理量子退火凭借独特的量子隧穿效应可跳出传统智能算法极易陷入的局部极值,可视为一类具有全局寻优能力的人工智能算法.本文研究了两类基于量子退火的RSA公钥密码攻击算法(分解大整数N=pq):一是将密码攻击数学方法转为组合优化问题或指数级空间搜索问题,通过Ising模型或QUBO模型求解,提出了乘法表的高位优化模型,建立新的降维公式,使用D-Wave Advantage分解了 200万整数2269753.大幅度超过普渡大学、Lockheed Martin和富士通等实验指标,且Ising模型系数h范围缩小了 84%,系数J范围缩小了 80%,极大地提高了分解成功率,这是一类完全基于D-Wave量子计算机的攻击算法;二是基于量子退火算法融合密码攻击数学方法优化密码部件的攻击,采用量子退火优化CVP问题求解,通过量子隧穿效应获得比Babai算法更近的向量,提高了 CVP问题中光滑对的搜索效率,在D-Wave Advantage上实现首次50比特RSA整数分解.实验表明,在通用量子计算机器件进展缓慢情况下,D-Wave表现出更好的现实攻击能力,且量子退火不存在NISQ量子计算机VQA算法的致命缺陷贫瘠高原问题:算法会无法收敛且无法扩展到大规模攻击.

Absorption properties and mechanism of lightweight and broadband electromagnetic wave-absorbing porous carbon by the swelling treatment

Bioderived carbon materials have garnered considerable interest in the fields of microwave absorption and shielding due to their reproducibility and environmental friendliness.In this study,KOH was evenly distributed on biomass Tremella using the swelling induction method,leading to the preparation of a three-dimensional network-structured hierarchical porous carbon(HPC)through carbonization.The achieved microwave absorption intensity is robust at-47.34 dB with a thin thickness of 2.1 mm.Notably,the widest effective absorption bandwidth,reaching 7.0 GHz(11–18 GHz),is attained at a matching thickness of 2.2 mm.The exceptional broadband and reflection loss performance are attributed to the 3D porous networks,interface effects,carbon network defects,and dipole relaxation.HPC has outstanding absorption characteristics due to its excellent impedance matching and high attenuation constant.The uniform pore structures considerably optimize the impedance-matching performance of the material,while the abundance of interfaces and defects enhances the dielectric loss,thereby improving the attenuation constant.Furthermore,the impact of carbonization temperature and swelling rate on microwave absorption performance was systematically investigated.This research presents a strategy for preparing absorbing materials using biomass-derived HPC,showcasing considerable potential in the field of electromagnetic wave absorption.

Structural Engineering of Hierarchical Magnetic/Carbon Nanocomposites via In Situ Growth for High-Efficient Electromagnetic Wave Absorption

Materials exhibiting high-performance electromagnetic wave absorption have garnered considerable scientific and technological attention,yet encounter significant challenges.Developing new materials and innovative structural design concepts is crucial for expanding the application field of electromagnetic wave absorption.Particularly,hierarchical structure engineering has emerged as a promising approach to enhance the physical and chemical properties of materials,providing immense potential for creating versatile electromagnetic wave absorption materials.Herein,an exceptional multi-dimensional hierarchical structure was meticulously devised,unleashing the full microwave attenuation capabilities through in situ growth,selfreduction,and multi-heterogeneous interface integration.The hierarchical structure features a three-dimensional carbon framework,where magnetic nanoparticles grow in situ on the carbon skeleton,creating a necklace-like structure.Furthermore,magnetic nanosheets assemble within this framework.Enhanced impedance matching was achieved by precisely adjusting component proportions,and intelligent integration of diverse interfaces bolstered dielectric polarization.The obtain Fe_(3)O_(4)-Fe nanoparticles/carbon nanofibers/Al-Fe_(3)O_(4)-Fe nanosheets composites demonstrated outstanding performance with a minimum reflection loss(RLmin)value of−59.3 dB and an effective absorption bandwidth(RL≤−10 dB)extending up to 5.6 GHz at 2.2 mm.These notable accomplishments offer fresh insights into the precision design of high-efficient electromagnetic wave absorption materials.

Tracking Regulatory Mechanism of Trace Fe on Graphene Electromagnetic Wave Absorption

Polarization and conductance losses are the fundamental dielectric attenuation mechanisms for graphene-based absorbers, but it is not fully understood in revealing the loss mechanism of affect graphene itself. For the first time, the reduced graphene oxide(RGO) based absorbers are developed with regulatory absorption properties and the absorption mechanism of RGO is mainly originated from the carrier injection behavior of trace metal Fe nanosheets on graphene. Accordingly, the minimum reflection loss(RLmin) of Fe/RGO-2composite reaches-53.38 dB(2.45 mm), and the effective absorption bandwidth achieves 7.52 GHz(2.62 mm) with lower filling loading of 2 wt%. Using off-axis electron hologram testing combined with simulation calculation and carrier transport property experiments, we demonstrate here the carrier injection behavior from Fe to graphene at the interface and the induced charge accumulation and rearrangement, resulting in the increased interfacial and dipole polarization and the conductance loss. This work has confirmed that regulating the dielectric property of graphene itself by adding trace metals can not only ensure good impedance matching, but also fully exploit the dielectric loss ability of graphene at low filler content,which opens up an efficient way for designing lightweight absorbers and may be extended to other types materials.

Interface Engineering of Titanium Nitride Nanotube Composites for Excellent Microwave Absorption at Elevated Temperature

Currently,the microwave absorbers usually suffer dreadful electromagnetic wave absorption(EMWA)performance damping at elevated temperature due to impedance mismatching induced by increased conduction loss.Consequently,the development of high-performance EMWA materials with good impedance matching and strong loss ability in wide temperature spectrum has emerged as a top priority.Herein,due to the high melting point,good electrical conductivity,excellent environmental stability,EM coupling effect,and abundant interfaces of titanium nitride(TiN)nanotubes,they were designed based on the controlling kinetic diffusion procedure and Ostwald ripening process.Benefiting from boosted heterogeneous interfaces between TiN nanotubes and polydimethylsiloxane(PDMS),enhanced polarization loss relaxations were created,which could not only improve the depletion efficiency of EMWA,but also contribute to the optimized impedance matching at elevated temperature.Therefore,the TiN nanotubes/PDMS composite showed excellent EMWA performances at varied temperature(298-573 K),while achieved an effective absorption bandwidth(EAB)value of 3.23 GHz and a minimum reflection loss(RLmin)value of−44.15 dB at 423 K.This study not only clarifies the relationship between dielectric loss capacity(conduction loss and polarization loss)and temperature,but also breaks new ground for EM absorbers in wide temperature spectrum based on interface engineering.

Analysis of explosion wave interactions and rock breaking effects during dual initiation

In blasting engineering, the location and number of detonation points, to a certain degree, regulate the propagation direction ofthe explosion stress wave and blasting effect. Herein, we examine the explosion wave field and rock breaking effect in terms of shockwave collision, stress change of the blast hole wall in the collision zone, and crack propagation in the collision zone. The produced shockwave on the collision surface has an intensity surpassing the sum of the intensities of the two colliding explosion shock waves. At the collisionlocation, the kinetic energy is transformed into potential energy with a reduction in particle velocity at the wave front and the wavefront pressure increases. The expansion form of the superposed shock wave is dumbbell-shaped, the shock wave velocity in the collisionarea is greater than the radial shock wave velocity, and the average propagation angle of the explosion shock waves is approximately 60°.Accordingly, a fitted relationship between blast hole wall stress and explosion wave propagation angle in the superposition area is plotted.Under the experimental conditions, the superimposed explosion wave stress of the blast hole wall is approximately 1.73 times the singleexplosionwave incident stress. The results of the model test and numerical simulations reveal that large-scale radial fracture cracks weregenerated on the blast hole wall in the superimposed area, and the width of the crack increased. The width of the large-scale radial fracturecracks formed by a strong impact is approximately 5% of the blast hole length. According to the characteristics of blast hole wallcompression, the mean peak pressures of the strongly superimposed area are approximately 1.48 and 1.84 times those of the weakly superimposedand nonsuperimposed areas, respectively.

WAVE1调控脂多糖诱导的巨噬细胞线粒体代谢异常和炎症反应的机制研究

目的探讨WASP家族富含脯氨酸同源蛋白1(Wiskott-Aldrich syndrome protein family verprolinhomologous protein 1,WAVE1)调控脂多糖(lipopolysaccharide,LPS)诱导的巨噬细胞线粒体代谢异常和炎症反应的机制。方法构建过表达WAVE1的巨噬细胞系(小鼠BMDM和人THP-1细胞),LPS(500 ng/mL)处理巨噬细胞模拟脓毒症炎症反应,实验分为两部分。第一部分设立对照组、LPS组、空载质粒(LPS+oe-NC)组、WAVE1过表达(LPS+oe-WAVE1)组;第二部分设立LPS组、LPS+oe-NC组、LPS+oe-WAVE1组、外源性高迁移率族蛋白1(high mobility group box-1,HMGB1)干预(LPS+oe-WAVE1+HMGB1)组。采用RT-PCR法测定线粒体DNA含量,RT-qPCR法检测WAVE1、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、白介素(interleukin,IL)-1β、IL-6 mRNA表达,免疫印迹法检测WAVE1、己糖激酶2、丙酮酸激酶M2蛋白表达,ELISA法检测TNF-α、IL-1β、IL-6、HMGB1含量,JC-1染色法检测线粒体膜电位,Seahorse XP96法检测耗氧率和细胞外酸化率,MitoSOX探针检测线粒体活性氧水平,2-NBDG法检测葡萄糖摄取水平,试剂盒检测丙酮酸激酶活性、乳酸、三磷酸腺苷、HMGB1水平。结果与对照组相比,LPS组WAVE1蛋白和mRNA表达、线粒体膜电位、耗氧率、线粒体DNA含量降低(P<0.05),TNF-α、IL-1β、IL-6含量和mRNA表达、线粒体活性氧、葡萄糖摄取、乳酸、三磷酸腺苷、己糖激酶2和丙酮酸激酶M2蛋白表达水平以及细胞外酸化率、丙酮酸激酶活性、HMGB1释放量升高(P<0.05);与LPS+oe-NC组相比,LPS+oe-WAVE1组WAVE1蛋白和mRNA表达、线粒体膜电位、耗氧率、线粒体DNA含量升高(P<0.05),TNF-α、IL-1β、IL-6含量和mRNA表达、线粒体活性氧、葡萄糖摄取、乳酸、三磷酸腺苷、己糖激酶2和丙酮酸激酶M2蛋白表达水平以及细胞外酸化率、丙酮酸激酶活性、HMGB1释放量降低(P<0.05)。与LPS+oe-WAVE1组比较,LPS+oe-WAVE1+HMGB1组葡萄糖摄取、乳酸、三磷酸腺苷水平及细胞外酸化率升高(P<0.05)。结论WAVE1通过调控炎症因子释放、线粒体代谢及HMGB1释放参与调控LPS诱导的巨噬细胞炎症反应。