An attempt was made to preserve mango slices by treating with 2% calcium chloride solution followed by dipping into sugar syrup of different concentrations i.e. 50°, 60° and 70 °Brix respectively. The t...An attempt was made to preserve mango slices by treating with 2% calcium chloride solution followed by dipping into sugar syrup of different concentrations i.e. 50°, 60° and 70 °Brix respectively. The treated slices were subjected for drying in different modes of drying (oven, microwave oven and cabinet tray drying) and analyzed for various physico-chemical and organoleptic quality characteristics. The study revealed that the osmo-air dried slices of mango produced with partial dehydration facilitated by osmotic agent (sugar syrup of 60 °Brix. A fruit: sugar syrup ratio of 1:4 (w/v) for 18 h at 40 ℃ temperature) followed by mechanical drying showed superiority in sensorial quality attributes over other concentrations of ingredients and the rest of the modes of drying. The good quality osmotically dehydrated mango slices could be preserved with maximum retentions of vitamins with better dehydration, rehydration and sensorial quality characteristics.展开更多
Objective To establish a fast and sensitive method for the detection of 8-hydroxy-2’-deoxyguanosine (8-OHdG) in precision-cut rat liver slices by HPLC-MS/MS and to investigate isoniazid (INH) -induced oxidative D...Objective To establish a fast and sensitive method for the detection of 8-hydroxy-2’-deoxyguanosine (8-OHdG) in precision-cut rat liver slices by HPLC-MS/MS and to investigate isoniazid (INH) -induced oxidative DNA damage. Methods Precision-cut liver slices (300 μm) were prepared from male rats, and incubated with INH (0.018 mol/L) for 2 h after 1 h preincubation. DNA in the slices was extracted and digested into free nucleosides at 37℃ . The samples were injected into HPLC-MS/MS after the proteins were removed. The level of oxidative DNA damage was estimated using the ratio of 8-OHdG to deoxyguanosine (dG). Results The limit of detection of 8-OHdG was 1 ng/mL (S/N=3) and the intra-assay relative standard variation was 3.38% when one transition 284.3/168.4 was used as a quantifier and another two transitions 284.3/140.2, 306.1/190.2 as qualifiers. 8-OHdG and dG were well separated, as indicated by elution at 10.02 and 7.37 min, respectively. INH significantly increased the ratio of 8-OHdG to dG in rat liver slices (P〈0.05). Conclusion 8-OHdG in precision-cut liver slices could be sensitively determined by HPLC-MS/MS. HPLC-MS/MS coupled with precision-cut tissue slices is a fast and reliable analytical technique to evaluate oxidative DNA damage of target tissues caused by procarcinogens and cytotoxins.展开更多
The effects of potato cultivar, frying temperature and slice thickness on oil uptake and sensory quality of potato crisps were investigated in four Kenyan cultivars. Potato tubers were peeled, washed and cut into slic...The effects of potato cultivar, frying temperature and slice thickness on oil uptake and sensory quality of potato crisps were investigated in four Kenyan cultivars. Potato tubers were peeled, washed and cut into slices of thickness 1.0 mm, 1.5 mm and 2.0 mm. Each size was fried at a constant temperature of 170 ℃ for 3-5 minutes. For frying temperature evaluation, the potatoes for all cultivars were cut into a uniform thickness of 1.5 mm and fried at temperatures of 160, 170 and 180 ℃ for 2-5 minutes. Crisps made from the four cultivars differed significantly (P 〈 0.05) in oil absorbed which ranged from 35.12% in Dutch Robyjn to 36.52% in clone 391,691.96. Tuber dry matter differed significantly (P 〈 0.05) among the cultivars ranging from 20.99% in clone 391691.96 to 25.29% in variety Dutch Robyjn. Tuber dry matter content was found to be negatively correlated to oil content of crisps; oil content increased with decrease in dry matter content. For each cultivar, the oil content of crisps differed significantly (P 〈 0.05) with temperatures and was higher at frying temperatures of 160 ℃ and lowest at 180 ℃, respectively. The oil content was significantly (P 〈 0.05) higher in slices of 1.0 mm thick than in slices of 1.5 mm and 2.0 mm; the amount ofoil absorbed decreased with increase in slice thickness. There was significant correlation (P 〈 0.05, r = -0.834) between oil content as determined in the laboratory and sensory scores. Results showed that high dry matter, slice thickness and temperature of frying resulted in reduced oil absorption by crisps during processing.展开更多
In the late 1980s,superparamagnetic iron oxide nanoparticles(SPIO)moved into focus as contrast agents in magnetic resonance imaging(MRI),due to their strong relaxivity and resulting higher resolution of images.At ...In the late 1980s,superparamagnetic iron oxide nanoparticles(SPIO)moved into focus as contrast agents in magnetic resonance imaging(MRI),due to their strong relaxivity and resulting higher resolution of images.At the time,no one anticipated their high potential in basic research or for medical diagnostic andtreatment. Since then, SPIO have been evaluated notonly as spe- cific markers for MRI, but also for cell labeling and tracking (Li et al., 2013).展开更多
This paper suggests that a single class rather than methods should be used as the slice scope to compute class cohesion. First, for a given attribute, the statements in all methods that last define the attribute are c...This paper suggests that a single class rather than methods should be used as the slice scope to compute class cohesion. First, for a given attribute, the statements in all methods that last define the attribute are computed. Then, the forward and backward data slices for this attribute are generated by using the class as the slice scope and are combined to compute the corresponding class data slice. Finally, the class cohesion is computed based on all class data slices for the attributes. Compared to traditional cohesion metrics that use methods as the slice scope, the proposed metrics that use a single class as slice scope take into account the possible interactions between the methods. The experimental results show that class cohesion can be more accurately measured when using the class as the slice scope.展开更多
To improve the accuracy and speed in cycle-accurate power estimation, this paper uses multiple dimensional coefficients to build a Bayesian inference dynamic power model. By analyzing the power distribution and intern...To improve the accuracy and speed in cycle-accurate power estimation, this paper uses multiple dimensional coefficients to build a Bayesian inference dynamic power model. By analyzing the power distribution and internal node state, we find the deficiency of only using port information. Then, we define the gate level number computing method and the concept of slice, and propose using slice analysis to distill switching density as coefficients in a special circuit stage and participate in Bayesian inference with port information. Experiments show that this method can reduce the power-per-cycle estimation error by 21.9% and the root mean square error by 25.0% compared with the original model, and maintain a 700 + speedup compared with the existing gate-level power analysis technique.展开更多
[Objective] The aim was to investigate the effects of different drying temperatures on the physiochemical properties and antioxidant activity of balsam pear slices. [Method] Balsam pear slices were dried at different ...[Objective] The aim was to investigate the effects of different drying temperatures on the physiochemical properties and antioxidant activity of balsam pear slices. [Method] Balsam pear slices were dried at different hot air temperatures, 40, 50, 60, 70 and 80 ℃. [Result] The polyphenols content was highest (2.83 mg/g) in the balsam pear slices dried at 50 ℃, and the flavonoids content was highest (2.584 mg/g) in those dried at 60 ℃. Different drying temperatures had a great impact on the antioxidant capacity of polyphenols in balsam pear. The balsam pear slices dried at 50 ℃ showed the strongest capacity for scavenging DPPH free radicals with IC50 of 0.015 mg/ml, and those dried at 80 ℃ showed the strongest capacity for removing ABTS free radicals with IC50 of 0.0689 mg/ml. [Conclusion] The hot air temperature of 50 ℃ had the least impact on the quality of balsam pear slices.展开更多
The influences of exogenous H 2O 2 and salicylic acid (SA) treatments on the alternative respiratory pathway (ARP) in aged potato (Solanum tuberosum L.) tuber slices were compared. The results showed that both H 2O...The influences of exogenous H 2O 2 and salicylic acid (SA) treatments on the alternative respiratory pathway (ARP) in aged potato (Solanum tuberosum L.) tuber slices were compared. The results showed that both H 2O 2(5.0 mmol/L) and SA (0.1 mmol/L) treatments had a significant inducing effect on ARP capacity (V alt ) and its ratio to total respiration (V alt /V t) in potato slices aged for 24 h. With a monoclonal antibody against the alternative oxidase (AOX), Western blotting results showed that both H 2O 2 and SA treatments increased the AOX expression levels in aged potato tuber slices. However, the results of oxygen isotope discrimination experiments showed that H 2O 2 had no influence on the in vivo ARP activity (ρV alt ) and its contribution to V t(expressed as ρV alt /V t) in potato slices aged for 24 h, but SA had a significant influence on the ρV alt and ρV alt /V t values of the aged potato tuber slices. These results indicate that there are differences between the effects of H 2O 2 and SA on ARP in plant tissues. Both of them possess the ability to induce ARP capacity through inducing AOX expression. However, SA can simultaneously stimulate the operation of ARP, but H 2O 2 can not.展开更多
Objective To identify the protective effect of lipopolysaccharide (LPS) preconditioning against LPS-induced inflammatory damage in dopaminergic neurons of midbrain slice culture and the possible mechanisms. Methods ...Objective To identify the protective effect of lipopolysaccharide (LPS) preconditioning against LPS-induced inflammatory damage in dopaminergic neurons of midbrain slice culture and the possible mechanisms. Methods After cultured in vitro for 14 d, the rat organotypic midbrain slices were pretreated with different concentrations (0, 1, 3, 6 or 10 ng/mL) of LPS for 24 h followed by treatment with 100 ng/mL LPS for 72 h. The whole slice viability was detelmined by measurement of the activity of lactic acid dehydrogenase (LDH). Tyrosine hydroxylase-immunoreactive (TH-IR) neurons and CD 1 1 b/c equivalent-immunoreactive (OX-42-IR) microglia in the slices were observed by immunohistochemical method, and tumor necrosis factor-α (TNF-α levels in the culture media were detected by enzymelinked immunosorbent assays (ELISA). Results In the slices treated with 100 ng/mL LPS for 72 h, the number of TH-IR neurons reduced from 191± 12 in the control slices to 46±4, and the LDH activity elevated obviously (P 〈 0.01), along with remarkably increased number of OX-42-IR cells and production of TNF-α (P 〈 0.01). Preconditioning with 3 or 6 ng/mL LPS attenuated neuron loss (the number of TH-IR neurons increased to 126± 12 and 180± 13, respectively) and markedly reduced LDH levels (P 〈 0.05), accompanied by significant decreases of OX-42-IR microglia activation and TNF-α production (P 〈 0.05). Conclusion Low-dose LPS preconditioning could protect dopaminergic neurons against inflammatory damage in rat midbrain slice culture, and inhibition of microglial activation and reduction of the proinflammatory factor TNF-α production may contribute to this protective effect. Further understanding the underlying mechanism of LPS preconditioning may open a new window for treatment of Parkinson's disease.展开更多
Beamline BL17 U1 at Shanghai Synchrotron Radiation Facility is an energy-tunable macromolecular crystallography beamline that has been in user operation since 2009. Growing demand from the user community for a small b...Beamline BL17 U1 at Shanghai Synchrotron Radiation Facility is an energy-tunable macromolecular crystallography beamline that has been in user operation since 2009. Growing demand from the user community for a small beam and related experimental methods have motivated upgrades of the devices in the endstation.Minibeam modes have already been developed for operation. A self-integrated diffractometer reduces the sphere of confusion of the rotatory axis to 1μm. The new diffractometer is equipped with an upgraded on-axis viewing system that can improve the resolving power. Additionally,the area detector was also upgraded to the newest generation of detectors, the EIGER X 16 M, which can collect data at 133 Hz. After these upgrades, the endstation became virtually new. This paper covers the upgrade of the endstation devices and gives the first data collection results.展开更多
Wireless transmission method in wireless sensor networks has put forward higher requirements for private protection technology. According to the packet loss problem of private protection algorithm based on slice techn...Wireless transmission method in wireless sensor networks has put forward higher requirements for private protection technology. According to the packet loss problem of private protection algorithm based on slice technology, this paper proposes the data private protection algorithm with redundancy mechanism, which ensures privacy by privacy homomorphism mechanism and guarantees redundancy by carrying hidden data. Moreover,it selects the routing tree generated by CTP(Collection Tree Protocol) as routing path for data transmission. By dividing at the source node, it adds the hidden information and also the privacy homomorphism. At the same time,the information feedback tree is established between the destination node and the source node. In addition, the destination node immediately sends the packet loss information and the encryption key via the information feedback tree to the source node. As a result,it improves the reliability and privacy of data transmission and ensures the data redundancy.展开更多
Ultra-thick steep coal seam mining will inevitably lead to the increase of greater and violent ground subsidence and deformation.A subsidence control method by inversely-inclined slicing and upward mining is proposed ...Ultra-thick steep coal seam mining will inevitably lead to the increase of greater and violent ground subsidence and deformation.A subsidence control method by inversely-inclined slicing and upward mining is proposed in this paper.By this method,the sequence of collapse of overlying strata and the direction of propagation of strata movement are changed,the extent of roof-side deformation thereby is lessened,and boundary angle of roof-side subsidence is reduced by 5°-10°.The mechanism of this mining method for control of strata movement has been evidenced by numerical simulation and experiments with similarity materials.A subsidence prediction model based on the variation of mining influence propagation angle can be used to evaluate the surface movement and deformation of the mining method.The application of the method in No.3 Mine in Yaojie mining area has yielded the expected result.展开更多
In the 5th generation(5G)wireless communication networks,network slicing emerges where network operators(NPs)form isolated logical slices by the same cellular network infrastructure and spectrum resource.In coverage r...In the 5th generation(5G)wireless communication networks,network slicing emerges where network operators(NPs)form isolated logical slices by the same cellular network infrastructure and spectrum resource.In coverage regions of access points(APs)shared by slices,device to device(D2D)communication can occur among different slices,i.e.,one device acts as D2D relay for another device serving by a different slice,which is defined as slice cooperation in this paper.Since selfish slices will not help other slices by cooperation voluntarily and unconditionally,this paper designs a novel resource allocation scheme to stimulate slice cooperation.The main idea is to encourage slice to perform cooperation for other slices by rewarding it with higher throughput.The proposed incentive scheme for slice cooperation is formulated by an optimal problem,where cooperative activities are introduced to the objective function.Since optimal solutions of the formulated problem are long term statistics,though can be obtained,a practical online slice scheduling algorithm is designed,which can obtain optimal solutions of the formulated maximal problem.Lastly,the throughput isolation indexes are defined to evaluate isolation performance of slice.According to simulation results,the proposed incentive scheme for slice cooperation can stimulate slice cooperation effectively,and the isolation of slice is also simulated and discussed.展开更多
Position-spoofing-based attacks seriously threaten the security of Vehicular Ad Hoc Network(VANET).An effective solution to detect position spoofing is location verification.However,since vehicles move fast and the to...Position-spoofing-based attacks seriously threaten the security of Vehicular Ad Hoc Network(VANET).An effective solution to detect position spoofing is location verification.However,since vehicles move fast and the topology changes quickly in VANET,the static location verification method in Wireless Sensor Network(WSN) is not suitable for VANET.Taking into account the dynamic changing topology of VANET and collusion,we propose a Time-Slice-based Location Verification scheme,named TSLV,to resist position spoofing in VANET.Specifically,TSLV transforms the dynamic topology into static topology by time slice and each time slice corresponds to a verification process.The verifier can implement location verification for the corresponding prover.During the verification process,the verifier first filters out vehicles which provide unreasonably claimed locations,and then uses the Mean Square Error(MSE)-based cluster approach to separate the consistent vehicles by time slice,and uses the consistent set for its verification.In addition,security analysis and simulation show that TSLV can defend against the collusion attack effectively.展开更多
To enhance the capacity of the radar-reconnaissance interception receiver recognizing linear frequency modulated (LFM) at a low signal-noise ratio, this paper presents WignerHough transform (WHT) of the LFM signal and...To enhance the capacity of the radar-reconnaissance interception receiver recognizing linear frequency modulated (LFM) at a low signal-noise ratio, this paper presents WignerHough transform (WHT) of the LFM signal and its corresponding characteristics, derives the probability density functions of the LFM signal and Gaussian white noise within WHT based on entropy (WHTE), dimension under different assumptions and puts forward a WHT algorithm based on entropy of slice to improve the capacity of detecting the LFM signal. Entropy of the WHT domain slice is adopted to assess the information size of polar radius or angle slice, which is converted into the weight factor to weight every slice. Double-deck weight is used to weaken the influences of noise and disturbance terms and WHTE treatment and signal detection procedure are also summarized. The rationality of the algorithm is demonstrated through theoretical analysis and formula derivation, the efficiency of the algorithm is verified by simulation comparison between WHT, fractional Fourier transform and periodic WHT, and it is highlighted that the WHTE algorithm has better detection accuracy and range of application against strong noise background.展开更多
文摘An attempt was made to preserve mango slices by treating with 2% calcium chloride solution followed by dipping into sugar syrup of different concentrations i.e. 50°, 60° and 70 °Brix respectively. The treated slices were subjected for drying in different modes of drying (oven, microwave oven and cabinet tray drying) and analyzed for various physico-chemical and organoleptic quality characteristics. The study revealed that the osmo-air dried slices of mango produced with partial dehydration facilitated by osmotic agent (sugar syrup of 60 °Brix. A fruit: sugar syrup ratio of 1:4 (w/v) for 18 h at 40 ℃ temperature) followed by mechanical drying showed superiority in sensorial quality attributes over other concentrations of ingredients and the rest of the modes of drying. The good quality osmotically dehydrated mango slices could be preserved with maximum retentions of vitamins with better dehydration, rehydration and sensorial quality characteristics.
基金This research was supported by the National Natural Science Foundation of China (No. 30600773).
文摘Objective To establish a fast and sensitive method for the detection of 8-hydroxy-2’-deoxyguanosine (8-OHdG) in precision-cut rat liver slices by HPLC-MS/MS and to investigate isoniazid (INH) -induced oxidative DNA damage. Methods Precision-cut liver slices (300 μm) were prepared from male rats, and incubated with INH (0.018 mol/L) for 2 h after 1 h preincubation. DNA in the slices was extracted and digested into free nucleosides at 37℃ . The samples were injected into HPLC-MS/MS after the proteins were removed. The level of oxidative DNA damage was estimated using the ratio of 8-OHdG to deoxyguanosine (dG). Results The limit of detection of 8-OHdG was 1 ng/mL (S/N=3) and the intra-assay relative standard variation was 3.38% when one transition 284.3/168.4 was used as a quantifier and another two transitions 284.3/140.2, 306.1/190.2 as qualifiers. 8-OHdG and dG were well separated, as indicated by elution at 10.02 and 7.37 min, respectively. INH significantly increased the ratio of 8-OHdG to dG in rat liver slices (P〈0.05). Conclusion 8-OHdG in precision-cut liver slices could be sensitively determined by HPLC-MS/MS. HPLC-MS/MS coupled with precision-cut tissue slices is a fast and reliable analytical technique to evaluate oxidative DNA damage of target tissues caused by procarcinogens and cytotoxins.
文摘The effects of potato cultivar, frying temperature and slice thickness on oil uptake and sensory quality of potato crisps were investigated in four Kenyan cultivars. Potato tubers were peeled, washed and cut into slices of thickness 1.0 mm, 1.5 mm and 2.0 mm. Each size was fried at a constant temperature of 170 ℃ for 3-5 minutes. For frying temperature evaluation, the potatoes for all cultivars were cut into a uniform thickness of 1.5 mm and fried at temperatures of 160, 170 and 180 ℃ for 2-5 minutes. Crisps made from the four cultivars differed significantly (P 〈 0.05) in oil absorbed which ranged from 35.12% in Dutch Robyjn to 36.52% in clone 391,691.96. Tuber dry matter differed significantly (P 〈 0.05) among the cultivars ranging from 20.99% in clone 391691.96 to 25.29% in variety Dutch Robyjn. Tuber dry matter content was found to be negatively correlated to oil content of crisps; oil content increased with decrease in dry matter content. For each cultivar, the oil content of crisps differed significantly (P 〈 0.05) with temperatures and was higher at frying temperatures of 160 ℃ and lowest at 180 ℃, respectively. The oil content was significantly (P 〈 0.05) higher in slices of 1.0 mm thick than in slices of 1.5 mm and 2.0 mm; the amount ofoil absorbed decreased with increase in slice thickness. There was significant correlation (P 〈 0.05, r = -0.834) between oil content as determined in the laboratory and sensory scores. Results showed that high dry matter, slice thickness and temperature of frying resulted in reduced oil absorption by crisps during processing.
基金supported by deutsche Forschungsgemeinschaft Grant Klinische Forschungsgruppe 213 to JG
文摘In the late 1980s,superparamagnetic iron oxide nanoparticles(SPIO)moved into focus as contrast agents in magnetic resonance imaging(MRI),due to their strong relaxivity and resulting higher resolution of images.At the time,no one anticipated their high potential in basic research or for medical diagnostic andtreatment. Since then, SPIO have been evaluated notonly as spe- cific markers for MRI, but also for cell labeling and tracking (Li et al., 2013).
基金The National Natural Science Foundation of China(No.60425206,60633010)the High Technology Research and Development Program of Jiangsu Province(No.BG2005032)
文摘This paper suggests that a single class rather than methods should be used as the slice scope to compute class cohesion. First, for a given attribute, the statements in all methods that last define the attribute are computed. Then, the forward and backward data slices for this attribute are generated by using the class as the slice scope and are combined to compute the corresponding class data slice. Finally, the class cohesion is computed based on all class data slices for the attributes. Compared to traditional cohesion metrics that use methods as the slice scope, the proposed metrics that use a single class as slice scope take into account the possible interactions between the methods. The experimental results show that class cohesion can be more accurately measured when using the class as the slice scope.
文摘To improve the accuracy and speed in cycle-accurate power estimation, this paper uses multiple dimensional coefficients to build a Bayesian inference dynamic power model. By analyzing the power distribution and internal node state, we find the deficiency of only using port information. Then, we define the gate level number computing method and the concept of slice, and propose using slice analysis to distill switching density as coefficients in a special circuit stage and participate in Bayesian inference with port information. Experiments show that this method can reduce the power-per-cycle estimation error by 21.9% and the root mean square error by 25.0% compared with the original model, and maintain a 700 + speedup compared with the existing gate-level power analysis technique.
文摘[Objective] The aim was to investigate the effects of different drying temperatures on the physiochemical properties and antioxidant activity of balsam pear slices. [Method] Balsam pear slices were dried at different hot air temperatures, 40, 50, 60, 70 and 80 ℃. [Result] The polyphenols content was highest (2.83 mg/g) in the balsam pear slices dried at 50 ℃, and the flavonoids content was highest (2.584 mg/g) in those dried at 60 ℃. Different drying temperatures had a great impact on the antioxidant capacity of polyphenols in balsam pear. The balsam pear slices dried at 50 ℃ showed the strongest capacity for scavenging DPPH free radicals with IC50 of 0.015 mg/ml, and those dried at 80 ℃ showed the strongest capacity for removing ABTS free radicals with IC50 of 0.0689 mg/ml. [Conclusion] The hot air temperature of 50 ℃ had the least impact on the quality of balsam pear slices.
文摘The influences of exogenous H 2O 2 and salicylic acid (SA) treatments on the alternative respiratory pathway (ARP) in aged potato (Solanum tuberosum L.) tuber slices were compared. The results showed that both H 2O 2(5.0 mmol/L) and SA (0.1 mmol/L) treatments had a significant inducing effect on ARP capacity (V alt ) and its ratio to total respiration (V alt /V t) in potato slices aged for 24 h. With a monoclonal antibody against the alternative oxidase (AOX), Western blotting results showed that both H 2O 2 and SA treatments increased the AOX expression levels in aged potato tuber slices. However, the results of oxygen isotope discrimination experiments showed that H 2O 2 had no influence on the in vivo ARP activity (ρV alt ) and its contribution to V t(expressed as ρV alt /V t) in potato slices aged for 24 h, but SA had a significant influence on the ρV alt and ρV alt /V t values of the aged potato tuber slices. These results indicate that there are differences between the effects of H 2O 2 and SA on ARP in plant tissues. Both of them possess the ability to induce ARP capacity through inducing AOX expression. However, SA can simultaneously stimulate the operation of ARP, but H 2O 2 can not.
基金the Foundation of Beijing Municipal Commission of Education,China (No.200410025011)
文摘Objective To identify the protective effect of lipopolysaccharide (LPS) preconditioning against LPS-induced inflammatory damage in dopaminergic neurons of midbrain slice culture and the possible mechanisms. Methods After cultured in vitro for 14 d, the rat organotypic midbrain slices were pretreated with different concentrations (0, 1, 3, 6 or 10 ng/mL) of LPS for 24 h followed by treatment with 100 ng/mL LPS for 72 h. The whole slice viability was detelmined by measurement of the activity of lactic acid dehydrogenase (LDH). Tyrosine hydroxylase-immunoreactive (TH-IR) neurons and CD 1 1 b/c equivalent-immunoreactive (OX-42-IR) microglia in the slices were observed by immunohistochemical method, and tumor necrosis factor-α (TNF-α levels in the culture media were detected by enzymelinked immunosorbent assays (ELISA). Results In the slices treated with 100 ng/mL LPS for 72 h, the number of TH-IR neurons reduced from 191± 12 in the control slices to 46±4, and the LDH activity elevated obviously (P 〈 0.01), along with remarkably increased number of OX-42-IR cells and production of TNF-α (P 〈 0.01). Preconditioning with 3 or 6 ng/mL LPS attenuated neuron loss (the number of TH-IR neurons increased to 126± 12 and 180± 13, respectively) and markedly reduced LDH levels (P 〈 0.05), accompanied by significant decreases of OX-42-IR microglia activation and TNF-α production (P 〈 0.05). Conclusion Low-dose LPS preconditioning could protect dopaminergic neurons against inflammatory damage in rat midbrain slice culture, and inhibition of microglial activation and reduction of the proinflammatory factor TNF-α production may contribute to this protective effect. Further understanding the underlying mechanism of LPS preconditioning may open a new window for treatment of Parkinson's disease.
基金supported by the Maintenance and Renovation Project for the major infrastructure of the Chinese Academy of Sciencesthe Operation Project of SSRF
文摘Beamline BL17 U1 at Shanghai Synchrotron Radiation Facility is an energy-tunable macromolecular crystallography beamline that has been in user operation since 2009. Growing demand from the user community for a small beam and related experimental methods have motivated upgrades of the devices in the endstation.Minibeam modes have already been developed for operation. A self-integrated diffractometer reduces the sphere of confusion of the rotatory axis to 1μm. The new diffractometer is equipped with an upgraded on-axis viewing system that can improve the resolving power. Additionally,the area detector was also upgraded to the newest generation of detectors, the EIGER X 16 M, which can collect data at 133 Hz. After these upgrades, the endstation became virtually new. This paper covers the upgrade of the endstation devices and gives the first data collection results.
基金sponsored by the National Key R&D Program of China(No.2018YFB1003201)the National Natural Science Foundation of China(No.61672296,No.61602261)Major Natural Science Research Projects in Colleges and Universities of Jiangsu Province(No.18KJA520008)
文摘Wireless transmission method in wireless sensor networks has put forward higher requirements for private protection technology. According to the packet loss problem of private protection algorithm based on slice technology, this paper proposes the data private protection algorithm with redundancy mechanism, which ensures privacy by privacy homomorphism mechanism and guarantees redundancy by carrying hidden data. Moreover,it selects the routing tree generated by CTP(Collection Tree Protocol) as routing path for data transmission. By dividing at the source node, it adds the hidden information and also the privacy homomorphism. At the same time,the information feedback tree is established between the destination node and the source node. In addition, the destination node immediately sends the packet loss information and the encryption key via the information feedback tree to the source node. As a result,it improves the reliability and privacy of data transmission and ensures the data redundancy.
基金sponsored by the National Natural Science Foundation of China(Nos.51574242 and 5097412).
文摘Ultra-thick steep coal seam mining will inevitably lead to the increase of greater and violent ground subsidence and deformation.A subsidence control method by inversely-inclined slicing and upward mining is proposed in this paper.By this method,the sequence of collapse of overlying strata and the direction of propagation of strata movement are changed,the extent of roof-side deformation thereby is lessened,and boundary angle of roof-side subsidence is reduced by 5°-10°.The mechanism of this mining method for control of strata movement has been evidenced by numerical simulation and experiments with similarity materials.A subsidence prediction model based on the variation of mining influence propagation angle can be used to evaluate the surface movement and deformation of the mining method.The application of the method in No.3 Mine in Yaojie mining area has yielded the expected result.
基金supported by Beijing Natural Science Foundation under Grant number L172049the National Science and CAS Engineering Laboratory for Intelligent Agricultural Machinery Equipment GC201907-02
文摘In the 5th generation(5G)wireless communication networks,network slicing emerges where network operators(NPs)form isolated logical slices by the same cellular network infrastructure and spectrum resource.In coverage regions of access points(APs)shared by slices,device to device(D2D)communication can occur among different slices,i.e.,one device acts as D2D relay for another device serving by a different slice,which is defined as slice cooperation in this paper.Since selfish slices will not help other slices by cooperation voluntarily and unconditionally,this paper designs a novel resource allocation scheme to stimulate slice cooperation.The main idea is to encourage slice to perform cooperation for other slices by rewarding it with higher throughput.The proposed incentive scheme for slice cooperation is formulated by an optimal problem,where cooperative activities are introduced to the objective function.Since optimal solutions of the formulated problem are long term statistics,though can be obtained,a practical online slice scheduling algorithm is designed,which can obtain optimal solutions of the formulated maximal problem.Lastly,the throughput isolation indexes are defined to evaluate isolation performance of slice.According to simulation results,the proposed incentive scheme for slice cooperation can stimulate slice cooperation effectively,and the isolation of slice is also simulated and discussed.
基金supported by National Natural Science Foundation of China under Grant No.60972036
文摘Position-spoofing-based attacks seriously threaten the security of Vehicular Ad Hoc Network(VANET).An effective solution to detect position spoofing is location verification.However,since vehicles move fast and the topology changes quickly in VANET,the static location verification method in Wireless Sensor Network(WSN) is not suitable for VANET.Taking into account the dynamic changing topology of VANET and collusion,we propose a Time-Slice-based Location Verification scheme,named TSLV,to resist position spoofing in VANET.Specifically,TSLV transforms the dynamic topology into static topology by time slice and each time slice corresponds to a verification process.The verifier can implement location verification for the corresponding prover.During the verification process,the verifier first filters out vehicles which provide unreasonably claimed locations,and then uses the Mean Square Error(MSE)-based cluster approach to separate the consistent vehicles by time slice,and uses the consistent set for its verification.In addition,security analysis and simulation show that TSLV can defend against the collusion attack effectively.
基金supported by the Aeronautical Science Fund of China(201455960252015209619)
文摘To enhance the capacity of the radar-reconnaissance interception receiver recognizing linear frequency modulated (LFM) at a low signal-noise ratio, this paper presents WignerHough transform (WHT) of the LFM signal and its corresponding characteristics, derives the probability density functions of the LFM signal and Gaussian white noise within WHT based on entropy (WHTE), dimension under different assumptions and puts forward a WHT algorithm based on entropy of slice to improve the capacity of detecting the LFM signal. Entropy of the WHT domain slice is adopted to assess the information size of polar radius or angle slice, which is converted into the weight factor to weight every slice. Double-deck weight is used to weaken the influences of noise and disturbance terms and WHTE treatment and signal detection procedure are also summarized. The rationality of the algorithm is demonstrated through theoretical analysis and formula derivation, the efficiency of the algorithm is verified by simulation comparison between WHT, fractional Fourier transform and periodic WHT, and it is highlighted that the WHTE algorithm has better detection accuracy and range of application against strong noise background.