Effects of Slow-release Nitrogen Fertilizer on Yield and Nitrogen Accumulation of Summer Maize in Shajiang Black Soil Area

[Objectives] This study was conducted to verify the field application effect of slow-release nitrogen fertilizer on summer maize in Shajiang black soil area by simultaneous sowing and fertilization, and explore the application scope and nitrogen metabolism mechanism, so as to lay a foundation for fertilizer reduction and efficiency improvement. [Methods] With maize variety Beiqing 340 and sulfur-coated urea as experimental materials, five nitrogen application levels were set, namely, control (C0), slow-release nitrogen 70 kg/hm^(2) (C70), slow-release nitrogen 140 kg/hm^(2) (C140), slow-release nitrogen 210 kg/hm^(2) (C210) and slow-release nitrogen 280 kg/hm^(2) (C280). The phosphorus and potassium fertilizers were all in accordance with the unified standard. [Results] With the application rate of slow-release nitrogen increasing, the nitrogen accumulation in organs increased first and then decreased after tasseling stage of maize. In order to reduce the fertilizing amount and increase efficiency, 210 kg/hm^(2) of slow-release nitrogen fertilizer was the best fertilizing amount for summer maize in Shajiang black soil area. [Conclusions] This study provides reference for fertilizer reduction, efficiency improvement and sustainable development of summer maize in Shajiang black soil area.

Optimization of slow-release fertilizer application improves lotus rhizome quality by affecting the physicochemical properties of starch

To achieve the dual goals of high yield and good quality with low environmental costs,slow-release fertilizer(SRF)has been widely used in lotus cultivation as new type of fertilizer instead of traditional nitrogen fertilizer.However,the optimal amount of SRF and how it would promote lotus rhizome quality remain unclear.This study was designed to investigate the photosynthetic characteristics and the synthesis,accumulation,and physicochemical properties of lotus rhizome starches under six SRF levels(CK,S1,S2,S3,S4,and S5).Compared with CK(0 kg ha^(–1)),the net photosynthetic rate(P_(n))and SPAD values of leaves remained at higher levels under SRF treatment.Further research showed that SRF increased the lotus rhizome yield,the contents of amylose,amylopectin,and total starch,and the number of starch granules.Among the six SRF levels,S3(1035 kg ha^(–1))showed the greatest difference from CK and produced the highest levels.With the increasing SRF levels,the peak,hot and final viscosities decreased at first and then increased,but the setback viscosity and pasting temperature increased.In order to interpret these changes at the molecular level,the activities of key enzymes and relative expression levels of starch accumulation related genes were analyzed.Each of these parameters also increased under SRF treatment,especially under the S3 treatment.The results of this study show that SRF,especially S3(1035 kg ha^(–1)),is a suitable fertilizer option for lotus planting which can improve lotus rhizome quality by affecting starch accumulations related enzymes and genes.These results will be useful for SRF application to high-quality lotus rhizome production with low environmental costs.

Impacts and Demonstration Effects of Applying Long-acting Slow-release Fertilizer on Economic Yield of Peanut

[Objectives]To study the impacts and demonstration effects of long-acting slow-release fertilizer application on economic yield of peanut.[Methods]The 25,30,35,40,45,50 kg of long-acting slow-release fertilizers were applied to 667 m 2 of peanuts,and different amounts of urea were applied together.[Results]Applying 40 kg of long-acting slow-release fertilizer and 10.45 kg of urea had the best effect.Compared with the application of ordinary compound fertilizers,the plants did not age prematurely,the leaf diseases were mild,the stems and leaves remained dark green when harvested,and the stems and leaves had a longer functional period.Bearing shoots increased by 1.7,single-plant full pods increased by 2.4,double-seed peanuts increased by 3.2,empty pods decreased by 0.5,and single-seed peanuts decreased by 0.7.The experimental demonstration results show that the spring-sowed peanuts had an average yield increase of 29.0-67.2 kg/667 m 2,and the yield increase rate was 7.35%-16.89%,and the difference was extremely significant.[Conclusions]In the high-yield cultivation of peanuts,the application of long-acting slow-release fertilizer can be promoted to improve peanut production.

Effects of Slow-release Fertilizer and Balanced Fertilization on Nitrogen, Phosphorus and Potassium Uptake in Peppers

Field plot experiment was conducted to study the effects of two slow-re- lease fertilizers and balanced fertilization on dry matter accumulation, yield, fertilizer use efficiency, nitrogen, phosphorus and potassium uptake of peppers at Jiangna Town, Yanshan County, Yunnan Province in 2011. The results showed that the dry matter accumulation in dried pepper plant, pepper yield, nitrogen, phosphorus, potassium uptake in peppers were significantly increased in all the fertilizer treat- ments, compared with those in control （no fertilizer）. Compared with conventional fertilization, balanced fertilization, slow-release compound fertilizer and slow-release urea fertilizer significantly increased dried pepper economic output by 20.94%, 17.5% and 14.54%, nitrogen uptake in dried peppers by 21.53%,18.46% and 13.19%, phosphorus uptake in dried peppers by 14.08%, 15.76% and 10.44%, potassium uptake in dried peppers by 22.66%, 15.73% and 16.28%; they also in- creased nitrogen and potassium use efficiency, but reduced potassium use efficiency due to the increased potassium addition. In treatments with balanced fertilization, slow-release compound fertilizer and slow-release urea fertilizer, the nitrogen utiliza- tion was 5.84%, 7.14% and 8.33% higher and the phosphorus utilization was 3.32%, 3.27% and 2.47% higher than those in treatment with conventional fertiliza- tion. In addition, the nitrogen application could be reduced by 20%-50% by bal- anced fertilization and the two slow-release fertilizers, thereby reducing environmen- tal pollution. Slow-release fertilizers could also reduce the frequency of fertilization and labor costs.

Effects of Soil Nitrate Nitrogen Residues and Leaching for Different Kinds of Slow-release Nitrogen Fertilizers in Tall-fescue Soil

ObjectiveThe aim was to explore the movement of nitrate nitrogen in tall-fescue soils by different kinds of slow release nitrogen fertilizers. MethodBased on infiltration-tanks and test plots, a new and environment friendly fertilizer was explored. ResultThe results show that compared with urea treatment, slow-release nitrogen fertilizer treatments could reduce nitrate nitrogen content and leaching amount in soils. Compared with PCU30 and IU treatments, the PCU60 treatment became more efficient in reducing nitrate content and leaching amount in 0-90 cm soil layer. ConclusionIn summary, slow-release nitrogen fertilizer, which can reduce soil nitrate content and leaching losses, is a kind of novel fertilizer with high environmental benefit and promising application.

Preliminary Report on Application Effects of Slow-release Formula Fertilizer in Tea Plants

In order to accelerate the construction of green production mode for tea in Hubei Province, improve the yield of tea and use efficiency of fertilizers, and improve the quality of tea, application effects of special slow-release formula fertilizer in tea plants were investigated in Wuhan. The results showed that compared with conventional fertilization, application of tea plant-specific slow-release formula fertilizer improved the yield of tea by more than 30%. The amino acid content in tea was increased by 21.05% and 30.82%, respectively, and the income was increased 27 696 （33.88%） and 27 624 （33.79%） yuan/hm2, respectively.

Effects of Slow-release Nitrogen on Dry Matter Accumulation,Translocation and Yield of Summer Maize

[Objectives]This study was conducted to investigate the effects of slow-release nitrogen fertilizer on dry matter accumulation and translocation of summer maize.[Methods]With Zhoudan 9 as the test variety,six different treatment were set up:blank control(CK1),slow-release urea 75 kg/hm^(2)(C1),slow-release urea 150 kg/hm^(2)(C2),slow-release urea 225 kg/hm^(2)(C3),slow-release urea 300 kg/hm^(2)(C4)and ordinary urea 300 kg/hm^(2)(CK2),to study the change law of dry matter accumulation and translocation in summer maize.[Results]Treatment slow-release urea 225 kg/hm^(2)(C4)showed summer maize yield,dry matter translocation between organs,grain contribution rate and proportion of grain dry matter in the full ripe stage higher than other treatments.Considering the weight loss and cost factors,slow-release urea 225 kg/hm^(2)(C3)could be recommended as the fertilizing amount for summer maize.[Conclusions]This study provides theoretical reference for rational selection of fertilizers for reducing fertilizer application and increasing fertilizer efficiency,and for production of summer maize in Shajiang black soil region.

Comparison of Nutrient Release Characteristics from Coated Controlled/Slow-release Fertilizers

[Objective] The study aimed at comparing the characteristics of nutrient release from coated controlled/slow-release fertilizers,so as to provide theoretical reference for improving the farming practice and fertilizer application,reducing pollution in Baiyangdian Lake and the surrounding area.[Method] Soil column leaching method was used to study NH4-N and NO3-N leaching characteristics from five kinds of nitrogen fertilizers including three coated controlled/slow-release fertilizers.[Result] Resin and sulfur coated controlled/slow-release urea decreased NH4-N concentration obviously,controlled NO3-N release in early term of experiment,while increased the content of NH4-N ＋ NO3-N in middle-late periods.Compared with that of common urea,the NH4-N of resin and sulfur coated controlled/slow-release urea decreased by 98.5%,98.7% in early period,96.5%,97.4% in middle period,and 59.1%,81.75% in last period.[Conclusion] Resin and sulfur coated controlled/slow-release urea significantly reduced the NH4-N concentration,effectively controlled the NO3-N release in early stage and increased the supply of NO3-N in last period.

Hybrid rice yield response to potted-seedling machine transplanting and slow-release nitrogen fertilizer application combined with urea topdressing

Machine transplanting and the application of slow-release nitrogen(N) fertilizer(SRNF) have played vital roles in the modernization of rice production. We aimed to determine the effects of potted-seedling transplanting—a new machine-transplanting method—and SRNF on hybrid rice yields. A 2-year splitplot experiment(2016–2017) was conducted in Meishan, Sichuan province, China, using two machinetransplanting methods(potted-seedling and blanket-seedling) and three N treatments. Total green leaf area, high-effective leaf area and its rate at heading, net photosynthetic rate of flag leaves 7 days after heading, glutamate synthase(GOGAT) and glutamine synthase(GS) activity after heading, dry matter production, and N accumulation at heading and maturity increased under the potted-seedling method or 70% SRNF as a base + 30% urea application at the panicle initiation stage(SBUP). Stem diameter and number of small and of all vascular bundles at the neck–panicle node in potted-seedling plants increased as a result of increasing numbers of effective panicles, secondary branches, and spikelets. In pottedseedling plants, treatment with SBUP increased the number of large and total vascular bundles at the panicle–neck internode and the number of differentiated and surviving secondary branches and spikelets and decreased the number of ineffective tillers and degenerated secondary branches and spikelets. We conclude that the potted-seedling machine transplanting method and SRNF combined with urea topdressing can strengthen the source–sink relationship in rice, resulting in higher yields.

Effects of Slow-release Fertilizers and Sugarcane-specific Fertilizers on Yield and Quality of Sugarcane

The effects of slow-release fertilizers and sugarcane-specific fertilizers on the growth and quality of sugarcane were studied to provide a theoretical basis for cultivation of sugarcane with lower costs but higher efficiency. Field experiments were carried out in two major sugarcane areas in Guangxi and three fertilization treatments were studied: single application of compound fertilizers( treatment I),compound fertilizers + slow-release fertilizers( treatment II) and sugarcane-specific base fertilizers + sugarcane-specific topdressing( treatment III). Effects of equal fertilization conditions of treatment I,II and III on growth,yield and sugar of sugarcane were studied. The three fertilization treatments had little effects on emergence,tillering,and effective stems of sugarcane,but compared with the treatment of compound fertilizer( treatment I) with a ratio of N,P,and K of 1∶1∶1,treatment II using slow-release fertilizers as topdressing had better growth,higher plant height and stem diameter,so the yield was higher. Treatment III designed sugarcane-specific fertilizers with proper ratio according to fertilizer demands of sugarcane. Besides,the treatment III sugarcane-specific base fertilizers,containing certain amount of organic matters,could promote the sugar accumulation of sugarcane. Therefore,the sugarcane yield of treatment III was higher than that of treatment II. In conclusion,slow-release fertilizers and sugarcane-specific fertilizers can significantly increase sugarcane yield,especially sugarcane-specific fertilizers. Sugarcane-specific fertilizers have reasonable ratio and contain certain amount of organic matters,and can increase sugar content,obtain significant economic benefits,so it is worth popularization in large areas.

Effect of slow-release iron fertilizer on iron-deficiency chlorosis, yield and quality of Lilium davidii var.unicolor in a two-year field experiment

Iron deficiency chlorosis of Lilium davidii var. unicolor is often the case in practice in alkaline soils of northwest region of China. It is difficult to control iron chlorosis because of high cost and short effective work time of conventional iron fertilizers. In this study, a 2-year field experiment was conducted to evaluate the effects of two slow-release fertilizers on the suppression of iron deficiency chlorosis, soil chemical properties, and the yield and quality of L. davidii var. unicolor. Results show that both coated slow-release iron fertilizers and embedded slow-release iron fertilizer effectively controlled iron-deficiency chlorosis. The application of slow-release iron fertilizers significantly increased plant height and chlorophyll content of L. davidii var. unicolor at different growth stages. Furthermore, coated iron fertilizer application significantly increased starch, protein, soluble sugar and vitamin C content of L. davidii var. unicolor, and it also significantly improved total amino acid content, with increases in essential amino acids(Trp, Leu, Lys, Phe, Val, and Thr contents) and in nonessential amino acids(Asp, Glu, Cit, Ihs, Acc, Ala, Pro, and Cys contents). It was concluded that application of coated slow-release iron fertilizer could be a promising option for suppression of iron deficiency chlorosis and deserves further study.

Delaying application time of slow-release fertilizer increases soil rhizosphere nitrogen content,root activity,and grain yield of spring maize

Application of slow-release fertilizer(SF)is a nutrient-management measure aimed at improving maize nutrient use and yield and saving labor cost.One-time application of SF at sowing usually results in nutrient deficiency during the post-silking stage,owing to the long growth period of spring maize.This study was conducted to investigate the effects on spring maize of SF application stage(zero,three-,and six-leaf stages,designated as SF0,SF3,and SF6,respectively)on grain yield,total soil rhizosphere nitrogen(N)content,and root activity,in comparison with the conventional fertilization mode(CF,application of compound fertilizer at sowing time,and topdressing urea at six-leaf and tasseling stages)at the same fertilization level as the control.Compared with no fertilization(F0)and CF,SF increased grain number and weight.The maize cultivars Suyu 30(SY30)and Jiangyu 877(JY877)produced the highest grain yield and net return under SF6 treatment over the three years.SF6 increased enzymatic activities including oxidoreductase,hydrolase,transferase,and lyase in rhizosphere soil at silking(R1)and milking stages(R3).SF6 increased the total N contents of rhizosphere soil by 7.1%at R1 and 9.2%R3 stages compared with SF0.The activities of antioxidant enzymes in roots were increased under SF6 treatments at R1and R3.The mean root activities of SF0,SF3,and SF6 increased by 7.1%,12.8%,and 20.5%compared with CF at R1 and by 8.8%,13.0%,and 23.5%at R3.Delaying the application time of SF could increase grain yield by increasing total N content of rhizosphere soil,delaying root senescence,and increasing root activity at the late reproductive stage.Applying SF at the six-leaf stage is recommended as an effective fertilization strategy for the sustainable production of spring maize in southern China.

Candelilla Wax as Natural Slow-Release Matrix for Fertilizers Encapsulated by Spray Chilling

Efforts to improve crop yields with efficient use of fertilizers are needed to guarantee global food security.Enhanced slow-release fertilizer systems(SRFs)encapsulated in biodegradable matrices are being developed to address this global concern.From a wide range of strategies for SRFs development,we explored a natureinspired solution based on the plant cuticle model and its function as a membrane for water and nutrient transport control.Here,Candelilla wax,extracted from Candelilla wild plants(Euphorbia antisyphilitica),is studied as a renewable slow-release matrix for fertilizers encapsulated by a modified spray chilling process.From this process,microencapsulates containing 40 wt%of phosphorus fertilizer are obtained with distinctive sizes and chemical characteristics,presenting a slow-release behavior.Considering the abovementioned features,novel insights into fertilizer release mechanisms based on plant cuticle models are discussed.

Used for the Study of Nitrogen Fertilizer Slow-Release Carrier Life Sludge

After the sewage treatment, putting the wet sludge in which the heavy metal content is extremely low, corrupt and broken straw, bentonite, urea in proportioning according to the certain ratio, mixing well-distributed, taking the shape of Nitrogen slow-release fertilizers, doing the dynamic bioleaching test by the method of Artificial rainfall simulation, researching the slow-release characteristic, water retention. When the mass ratio of the wet sludge whose water content is 82.5%: bentonite: corrupt and broken straw: urea is 62.5: 12.5: 12.5: 12.5, drip washing the 10g Nitrogen slow-release fertilizers by the 80ml distilled water after 48h, the residue rate of urea is 29.63%; Under the room temperature of 25 ℃, 77%RH, moisture evaporate 46.32% after 60 h. The results demonstrate that the slow-release fertilizer has a good release-effect of nitrogen and water conservation effect. It provides the basic for the development and application of the sewage in the aspect of Nitrogen slow-release fertilizers.

Effects of Long-term Application of Bag-controlled Slow-release Fertilizer on the Form and Availability of Phosphorus in Red Soil of Southern China

The insufficient supply of phosphorus is the main problem facing agriculture and forestry production in red soil region of southern China.To investigate whether applying bag-controlled slow-release fertilizer can improve the phosphorus availability and available phosphorus component content,with Gaofeng State-owned Eucalyptus Plantations Forest Farm in Guangxi red soil region as the research object,comparative test of different fertilizer types and fertilizer amount of bag-controlled slow-release fertilizer with conventional fertilization(G1),bag-controlled slow-release fertilizer with reducing fertilization(G2),traditional compound fertilizer(G3),basal fertilizer(G4),and no fertilization(G5)was designed.Soil phosphorus classification method was used to determine the content of phosphorus components in the soil,and redundancy analysis was used to study the effects of components on tree growth.The results showed that:compared with traditional fertilization,bag-controlled slow-release fertilizer significantly increased the contents of AP,Pi,Fe-P and other components in soil;the phosphorus activation coefficients of G2 and G1 were significantly higher than those of the other three treatments,and the order was G2>G1>G4>G5>G3;redundancy analysis showed that PAC and Fe-P had the most significant effect on plant growth among all phosphorus components.The application of bag-controlled slow-release fertilizer in red soil of eucalyptus plantation in southern China could promote the accumulation of phosphorus and increase the phosphorus activity.The mechanism of higher phosphorus use efficiency in bag-controlled slow-release fertilizer was mainly through the sustained phosphorus input and reducing phosphorus immobilization in soil by obstructing the contact of phosphorus with acid soil,to guarantee the phosphorus supply in tree growth process.

Effects of Nitrogen Fertilizer Level on Chlorophyll Fluorescence Characteristics in Flag Leaf of Super Hybrid Rice at Late Growth Stage

To compare the effects of slow-release nitrogen fertilizer at six different levels on the flag leaf chlorophyll fluorescence characteristics of super hybrid rice, a field fertilization experiment was conducted with super hybrid rice Y Liangyou 1 as a test material. The photosynthetic electron transport rate （ETR）, effective quantum yield （EQY）, photochemical quenching coefficient （qp）, and non-photochemical quenching coefficient （NPQ） of flag leaves were measured at the initial heading, full heading, 10 d after full heading and 20 d after full heading stages. Results showed that the values of ETR, EQY and qp increased with rice development from initial heading to 20 d after full heading, whereas the NPQ decreased. During the measured stages, ETR, EQY and qp increased initially and then decreased as nitrogen application amount increased, but they peaked at different nitrogen fertilizer levels. The maximum ETR and EQY values appeared at the treatment of 135 kg/hm2 N. In conclusion, the optimum nitrogen amount for chlorophyll fluorescence characteristics of super hybrid rice was 135-180 kg/hm2.

Co-incorporation of hydrotalcite and starch into biochar-based fertilizers for the synthesis of slow-release fertilizers with improved water retention

The unsatisfactory nutrient slow-release and water-retention performance of traditional biochar-based compound fertilizers(BCF)severely limit their practical application.Herein,a new type of slow-release fertilizer with high water retention was fabricated via the incorporation of hydrotalcite and starch into BCF,named as HS-BCF.The waterretention and nutrient releasing performance of the prepared HS-BCF and related nutrient slow-release mechanism were investigated.The results showed that the incorporation of hydrotalcite and starch into BCF could increase the soil water-retention ratio by 5-10%points.The accumulated N,P,and K leaching amounts of HS-BCF in soil within 30 days were 49.4%,13.3%,and 87.4%of BCF at most,respectively.Kinetic analysis indicated that the release of nutrients from HS-BCF was attributed to the coupling of the diffusion-controlled and relaxation-controlled mechanism.Moreover,hydrotalcite could bind with P in HS-BCF,contributing to the enhanced durability of P in HS-BCF.Finally,pot experiments showed that the N-P-K utilization efficiencies of HS-BCF were all higher than those of BCF due to a better synchronization between the nutrient release of HS-BCF and the uptake of tomato plants.Overall,the study may provide a promising strategy for simultaneously improving the water-retention and slow-release performance of traditional biochar-based fertilizers.

Preparation of a Slow Release Biofertilizer From a Polymeric Urea-Formaldehyde Matrix (PUFM)

Fertilizers industry faces the challenge of improving the efficiency of its products either by optimizing the fertilizers in use or by developing new types of them.During the last decade,controlled and slow release technologies have become more important.These technologies aim to increase the efficiency of the applied substance by increasing its action over time and avoiding losses of all kinds(leaching,volatilization).The main purpose of the current study was to obtain a slow release biofertilizer by incorporating microalgae into a polymeric urea-formaldehyde matrix(PUFM).The quantitative analysis of macronutrients and micronutrients in the microalgae was determined using different techniques including titration,UV and Atomic Adsorption Spectroscopy.The matrix and the formulation obtained(PUFM+CHLO)were also characterized by Infrared Spectroscopy(FTIR)and Scanning Electron Microscopy(SEM).The“in vitro”study showed a typical slow release behavior of nitrogen(N),phosphorus(P)and potassium(K)macronutrients.It was also shown that(PUFM+CHLO)formulation has the slowest macronutrients release time with a maximum release of 28%,26%y 46%for(N-P-K)macronutrients respectively during a period of 30 days.The"in vivo"study exposed the benefits of the biofertilizer formulation(PUFM+CHLO)from conventional commercial fertilizer(CF)(NPK-14-5-12).Due to the presence of nutrients of natural origin in microalgae,(PUFM+CHLO)shows ecological effects which could also developing sustainable agriculture systems.

长期单施NK肥条件下几种典型土壤磷的演化

为探讨我国典型土壤长期单施氮钾肥对土壤全磷和Olsen-P磷的影响,研究了不同气候条件、不同耕作制度下,7种典型土壤类型长期定位试验单施氮钾肥下土壤全磷和Olsen-P变化及其影响因素。结果表明,在长期单施氮钾肥条件下,除杭州水稻土全磷含量稳定在1.0g·kg-1左右外,其余6种耕作土壤全磷含量和土壤Olsen-P均呈显著下降趋势,全磷含量下降幅度在13.6%～44.9%之间;土壤Olsen-P下降速率比全磷高几倍,Olsen-P下降有一定阈值,这一阈值大约为5mg·kg-1。土壤全磷含量与土壤Olsen-P含量呈极显著直线相关关系。因此,长期单施氮钾肥在很大程度上减少了土壤磷素养分库。

不良IVF结局患者NK细胞信号通路调节FoxP3细胞表达的研究

目的探讨IVF不良结局患者叉头样转录因子P3(FoxP3)细胞表达情况及NK细胞信号通路调节机制。方法选取我院自2018年1月至2019年1月间收治的75例IVF不良结局患者作为研究对象,依据失败类型分成3组:实施IVF≥3次均未获得妊娠者(反复失败组)25例、实施IVF后仅生化妊娠患者(生化妊娠组)25例、实施IVF后妊娠12周内胚胎停止发育/自然流产者(流产胚停组)25例;选取同期生育1次健康未孕女性25例作为对照组。所有受检者均于卵泡末期采集静脉血液,EDTA抗凝后密度梯度离心法分离血液中的单个核细胞(PBMC),再用免疫磁珠法分离血液中的NK细胞、CD4+CD25-Treg细胞,流式细胞分析各组NK细胞亚群活性;实时定量PCR检测4组受检者CD4+CD25-Treg细胞中FoxP3 mRNA表达情况,并分析肿瘤坏死因子-α(TNF-α)诱导的NK细胞对于CD4+CD25-Treg细胞表达FoxP3的影响。结果与健康对照组比较,IVF不良结局各组CD56+、CD56+CD16+NK细胞水平均显著升高(P<0.05);IVF不良结局组中流产胚停组CD56+NK细胞水平显著高于其他两组(P<0.05)。IVF不良结局各组CD4+CD25-Treg细胞FoxP3 mRNA表达水平均显著低于健康对照组(P<0.05),且不良结局组中反复失败组、生化妊娠组CD4+CD25-Treg细胞FoxP3 mRNA表达水平显著低于流产胚停组(P<0.05)。体外刺激实验显示TNF-α诱导NK细胞下调CD4+CD25-Treg细胞FoxP3的表达(P<0.05)。结论IVF不良结局患者外周血中CD56+、CD56+CD16+NK细胞水平显著升高,而Treg细胞中Fox3 mRNA水平显著降低,且NK细胞能够下调Treg细胞中FoxP3的表达。