目的：研究宫颈癌发生发展过程中组织微小RNA-21表达及高危型HPV病毒载量变化的意义。方法选取2011年3月至2015年3月收治的宫颈病变并行宫颈活检的患者150例， CINⅠ组（ n ＝39）、CINⅡ组（ n ＝37）、CINⅢ组（ n ＝34），CC组（ n ...目的：研究宫颈癌发生发展过程中组织微小RNA-21表达及高危型HPV病毒载量变化的意义。方法选取2011年3月至2015年3月收治的宫颈病变并行宫颈活检的患者150例， CINⅠ组（ n ＝39）、CINⅡ组（ n ＝37）、CINⅢ组（ n ＝34），CC组（ n ＝40），分析miR-21的表达及HR-HPV载量与宫颈癌临床病理特征的联系，miR-21的表达与HR-HPV载量的相关性，不同宫颈病变中miR-21的表达及HR-HPV载量。结果宫颈癌患者肿瘤细胞低分化者miR-21表达（6．12±0．83）低于中分化者（8．17±0．65）及高分化者（11．54±1．29）（ P ＜0．05）；肿瘤细胞直径＞4 cm者HR-HPV载量（26．53±3．12）高于＜4 cm者（23．60±2．85）（ P ＜0．05），FIGO分期Ⅰ～ⅡA者HR-HPV载量（23．86±2．34）低于ⅡB～Ⅳ者（27．59±3．12）；宫颈癌HR-HPV阳性者miR-21表达（9．75±0．83）高于HR-HPV阴性者（5．47±0．46），差异有统计学意义（ P ＜0．05）；但miR-21表达及HR-HPV载量与宫颈癌年龄、FIGO分期、病理类型、淋巴转移、脉管浸润无明显相关性（ P ＞0．05）；CINⅠ组HR-HPV阳性患者（41．03％）分别低于 CINⅡ组（67．57％）、CINⅢ组（85．29％）及CC组（92．50％），CINⅠ组mi-R21相对表达水平（6．42±0．53）低于CINⅡ组（7．26±0．61）、CINⅢ组（8．24±0．65）及CC组（9．36±0．70），差异有统计学意义（ P ＜0．05）。结论微小RNA-21表达可反应宫颈癌细胞分化程度，高危型HPV病毒载量可反应宫颈癌FIGO分期及肿瘤直径范围，微小RNA-21表达与高危型HPV病毒载量呈正相关，联合检查微小RNA-21及高危型病毒载量能够提示宫颈癌前病变、宫颈癌的发生发展。展开更多
AIM: To investigate the incidence of Epstein-Barr virus-associated gastric cancer (EBV-GC) in Kazakhstan and to compare it with that in Russia, Western and Asian countries in order to evaluate the significance of epid...AIM: To investigate the incidence of Epstein-Barr virus-associated gastric cancer (EBV-GC) in Kazakhstan and to compare it with that in Russia, Western and Asian countries in order to evaluate the significance of epidemiopathologic and ethnic factors. METHODS: In situ hybridization (ISH) of EBV-encoded small RNA-1 (EBER-1) was used to identify the presence of EBER-1 signal in 139 formalin-fixed and paraffin-embedded GC tissues from Kazakhstan. RESULTS: EBER-1 expression was observed in the nuclei of 10% of the cases of GC (14/139), but not in the surrounding normal mucosa. The incidence of the diffuse type of EBV-GC was significantly higher in Kazakhstan (14%, 13/91) than that of the intestinal type (2%, 1/48). Furthermore, the incidence was significantly higher in males (14%, 12/89) than in females (3.7%, 2/53) from all countries. The overall incidence of EBV-GC increased from 6.7% in Asian countries to 8.7% in Russia, 10.1% in Kazakhstan and 16% in Western countries. CONCLUSION: Geographical differences in the incidence of EBV-GC may reflect the epidemiologic factors and/or dietary habits independent of histological type and sex.展开更多
文摘目的：研究宫颈癌发生发展过程中组织微小RNA-21表达及高危型HPV病毒载量变化的意义。方法选取2011年3月至2015年3月收治的宫颈病变并行宫颈活检的患者150例， CINⅠ组（ n ＝39）、CINⅡ组（ n ＝37）、CINⅢ组（ n ＝34），CC组（ n ＝40），分析miR-21的表达及HR-HPV载量与宫颈癌临床病理特征的联系，miR-21的表达与HR-HPV载量的相关性，不同宫颈病变中miR-21的表达及HR-HPV载量。结果宫颈癌患者肿瘤细胞低分化者miR-21表达（6．12±0．83）低于中分化者（8．17±0．65）及高分化者（11．54±1．29）（ P ＜0．05）；肿瘤细胞直径＞4 cm者HR-HPV载量（26．53±3．12）高于＜4 cm者（23．60±2．85）（ P ＜0．05），FIGO分期Ⅰ～ⅡA者HR-HPV载量（23．86±2．34）低于ⅡB～Ⅳ者（27．59±3．12）；宫颈癌HR-HPV阳性者miR-21表达（9．75±0．83）高于HR-HPV阴性者（5．47±0．46），差异有统计学意义（ P ＜0．05）；但miR-21表达及HR-HPV载量与宫颈癌年龄、FIGO分期、病理类型、淋巴转移、脉管浸润无明显相关性（ P ＞0．05）；CINⅠ组HR-HPV阳性患者（41．03％）分别低于 CINⅡ组（67．57％）、CINⅢ组（85．29％）及CC组（92．50％），CINⅠ组mi-R21相对表达水平（6．42±0．53）低于CINⅡ组（7．26±0．61）、CINⅢ组（8．24±0．65）及CC组（9．36±0．70），差异有统计学意义（ P ＜0．05）。结论微小RNA-21表达可反应宫颈癌细胞分化程度，高危型HPV病毒载量可反应宫颈癌FIGO分期及肿瘤直径范围，微小RNA-21表达与高危型HPV病毒载量呈正相关，联合检查微小RNA-21及高危型病毒载量能够提示宫颈癌前病变、宫颈癌的发生发展。
文摘AIM: To investigate the incidence of Epstein-Barr virus-associated gastric cancer (EBV-GC) in Kazakhstan and to compare it with that in Russia, Western and Asian countries in order to evaluate the significance of epidemiopathologic and ethnic factors. METHODS: In situ hybridization (ISH) of EBV-encoded small RNA-1 (EBER-1) was used to identify the presence of EBER-1 signal in 139 formalin-fixed and paraffin-embedded GC tissues from Kazakhstan. RESULTS: EBER-1 expression was observed in the nuclei of 10% of the cases of GC (14/139), but not in the surrounding normal mucosa. The incidence of the diffuse type of EBV-GC was significantly higher in Kazakhstan (14%, 13/91) than that of the intestinal type (2%, 1/48). Furthermore, the incidence was significantly higher in males (14%, 12/89) than in females (3.7%, 2/53) from all countries. The overall incidence of EBV-GC increased from 6.7% in Asian countries to 8.7% in Russia, 10.1% in Kazakhstan and 16% in Western countries. CONCLUSION: Geographical differences in the incidence of EBV-GC may reflect the epidemiologic factors and/or dietary habits independent of histological type and sex.
