RNA interference (RNAi) is triggered by the presence of a double-stranded RNA (dsRNA), and results in the silencing of homologous gene expression through the specific degradation of an mRNA containing the same sequenc...RNA interference (RNAi) is triggered by the presence of a double-stranded RNA (dsRNA), and results in the silencing of homologous gene expression through the specific degradation of an mRNA containing the same sequence. dsRNAmediated RNAi can be used in a wide variety of eucaryotes to induce the sequence-specific inhibition of gene expression.Synthetic 21-23 nucleotide (nt) small interfering RNA (siRNA) with 2 nt 3' overhangs was recently found to mediate efficient sequence-specific mRNA degradation in mammalian cells. Here, we studied the effects of synthetic siRNA duplexes targeted to SARS coronavirus structural proteins E, M, and N in a cell culture system. Among total 26 siRNA duplexes, we obtained 3 siRNA duplexes which could sequence-specifically reduce target genes expression over 80％ at the concentration of 60 nM in Vero E6 cells. The downregulation effect was in correlation with the concentrations of the siRNA duplexes in a range of 0～60 nM. Our results also showed that many inactive siRNA duplexes may be brought to life simply by unpairing the 5' end of the antisense strands. Results suggest that siRNA is capable of inhibiting SARS coronavirus genes expression and thus may be a new therapeutic strategy for treatment of SARS.展开更多
To satisfy practical requirements of high real-time accuracy and low computational complexity of synthetic aperture radar (SAR) image ship small target detection, this paper proposes a small ship target detection meth...To satisfy practical requirements of high real-time accuracy and low computational complexity of synthetic aperture radar (SAR) image ship small target detection, this paper proposes a small ship target detection method based on the improved You Only Look Once Version 3 (YOLOv3). The main contributions of this study are threefold. First, the feature extraction network of the original YOLOV3 algorithm is replaced with the VGG16 network convolution layer. Second, general convolution is transformed into depthwise separable convolution, thereby reducing the computational cost of the algorithm. Third, a residual network structure is introduced into the feature extraction network to reuse the shallow target feature information, which enhances the detailed features of the target and ensures the improvement in accuracy of small target detection performance. To evaluate the performance of the proposed method, many experiments are conducted on public SAR image datasets. For ship targets with complex backgrounds and small ship targets in the SAR image, the effectiveness of the proposed algorithm is verified. Results show that the accuracy and recall rate improved by 5.31% and 2.77%, respectively, compared with the original YOLOV3. Furthermore, the proposed model not only significantly reduces the computational effort, but also improves the detection accuracy of ship small target.展开更多
基金supported by the Grant No.2003AA208215 from the National High Technology Programs of Chinathe Grant No.30270311 from the National Natural Science Foundation of China.
文摘RNA interference (RNAi) is triggered by the presence of a double-stranded RNA (dsRNA), and results in the silencing of homologous gene expression through the specific degradation of an mRNA containing the same sequence. dsRNAmediated RNAi can be used in a wide variety of eucaryotes to induce the sequence-specific inhibition of gene expression.Synthetic 21-23 nucleotide (nt) small interfering RNA (siRNA) with 2 nt 3' overhangs was recently found to mediate efficient sequence-specific mRNA degradation in mammalian cells. Here, we studied the effects of synthetic siRNA duplexes targeted to SARS coronavirus structural proteins E, M, and N in a cell culture system. Among total 26 siRNA duplexes, we obtained 3 siRNA duplexes which could sequence-specifically reduce target genes expression over 80％ at the concentration of 60 nM in Vero E6 cells. The downregulation effect was in correlation with the concentrations of the siRNA duplexes in a range of 0～60 nM. Our results also showed that many inactive siRNA duplexes may be brought to life simply by unpairing the 5' end of the antisense strands. Results suggest that siRNA is capable of inhibiting SARS coronavirus genes expression and thus may be a new therapeutic strategy for treatment of SARS.
文摘To satisfy practical requirements of high real-time accuracy and low computational complexity of synthetic aperture radar (SAR) image ship small target detection, this paper proposes a small ship target detection method based on the improved You Only Look Once Version 3 (YOLOv3). The main contributions of this study are threefold. First, the feature extraction network of the original YOLOV3 algorithm is replaced with the VGG16 network convolution layer. Second, general convolution is transformed into depthwise separable convolution, thereby reducing the computational cost of the algorithm. Third, a residual network structure is introduced into the feature extraction network to reuse the shallow target feature information, which enhances the detailed features of the target and ensures the improvement in accuracy of small target detection performance. To evaluate the performance of the proposed method, many experiments are conducted on public SAR image datasets. For ship targets with complex backgrounds and small ship targets in the SAR image, the effectiveness of the proposed algorithm is verified. Results show that the accuracy and recall rate improved by 5.31% and 2.77%, respectively, compared with the original YOLOV3. Furthermore, the proposed model not only significantly reduces the computational effort, but also improves the detection accuracy of ship small target.
