近年来,赛博物理系统(Cyber-Physical Systems,CPS)随着工业4.0、智能制造的提出而成为行业热点话题,多CPS系统的智能互联是构成当今物联网(The Internet of Things&Services,IOTS)的基础。重点探索多个CPS之间的协同机制,提出了基...近年来,赛博物理系统(Cyber-Physical Systems,CPS)随着工业4.0、智能制造的提出而成为行业热点话题,多CPS系统的智能互联是构成当今物联网(The Internet of Things&Services,IOTS)的基础。重点探索多个CPS之间的协同机制,提出了基于总线型智能探头的多CPS互联互通模型以完成多CPS间的通信。在中航工业成飞数控加工厂建立了一套总线型CPS模型,实现了核心协议设计及编码设计,通过车间内各个MES子系统能够充分实现互联互通,以证明设计方法的可行性。展开更多
即时检测(Point of Care)技术在快速、灵敏、特异的检测疾病相关的生物因子中得到越来越多的关注。尤其是新兴的即时检测的平台的出现和推广,使得即时检测技术相比传统的诊断技术展现出巨大的优势。稀土上转换材料以其卓越的光学特性,...即时检测(Point of Care)技术在快速、灵敏、特异的检测疾病相关的生物因子中得到越来越多的关注。尤其是新兴的即时检测的平台的出现和推广,使得即时检测技术相比传统的诊断技术展现出巨大的优势。稀土上转换材料以其卓越的光学特性,在生物医学领域展现出巨大的潜力,是即时检测材料中冉冉升起的新星。从近年即时检测技术平台、稀土上转换材料等方面介绍了即时检测的发展,详细论述了检测试纸、智能手机和芯片实验室等检测平台、稀土上转换材料在即时检测中的应用。同时结合当前的市场分析,展望了稀土上转换材料在即时检测中未来发展的新趋势。展开更多
Nitroreductases(NTRs) are known to be able to metabolize nitro-substituted compounds in the presence of reduced nicotinamide adenine dinucleotide(NADH) as an electron donor. NTRs are present in a wide range of bacteri...Nitroreductases(NTRs) are known to be able to metabolize nitro-substituted compounds in the presence of reduced nicotinamide adenine dinucleotide(NADH) as an electron donor. NTRs are present in a wide range of bacterial genera and, to a lesser extent, in eukaryotes hypoxic tumour cells and tumorous tissues, which makes it an appropriate biomarker for an imaging target to detect the hypoxic status of cancer cells and potential bacterial infections. To evaluate the specific activation level of NTR, great efforts have been devoted to the development of fluorescent probes to detect NTR activities using fluorogenic methods to probe its behaviour in a cellular context; however, NTR-responsive MRI contrast agents are still by far underexplored. In this study, para-nitrobenzyl substituted T_1-weighted magnetic resonance imaging(MRI)contrast agent Gd-DOTA-PNB(probe 1) has been designed and explored for the possible detection of NTR.Our experimental results show that probe 1 could serve as an MRI-enhanced contrast agent for monitoring NTR activity. The in vitro response and mechanism of the NTR catalysed reduction of probe 1 have been investigated through LC–MS and MRI. Para-nitrobenzyl substituted probe 1 was catalytically reduced by NTR to the intermediate para-aminobenzyl substituted probe which then underwent a rearrangement elimination reaction to Gd-DOTA, generating the enhanced T1-weighted MR imaging. Further, LC–MS and MRI studies of living Escherichia coli have confirmed the NTR activity detection ability of probe 1 at a cellular level. This method may potentially be used for the diagnosis of bacterial infections.展开更多
随着无线局域网(WLAN,Wireless Local Area Networks)用户数据业务量的不断增长,无线局域网维护的工作量也不断增加,严重影响了无线局域网的性能和用户体验和满意度,为建设稳定、高效、安全的无线局域网,这里研究如何实现无线局域网热...随着无线局域网(WLAN,Wireless Local Area Networks)用户数据业务量的不断增长,无线局域网维护的工作量也不断增加,严重影响了无线局域网的性能和用户体验和满意度,为建设稳定、高效、安全的无线局域网,这里研究如何实现无线局域网热点智能安全监护系统(WASM,A Smart SecurityMonitoring System of WLAN AP)。首先详细讨论了无线局域网系统的原理和组成,其次讨论使用智能探针方式,实现无线局域网热点远程性能监护和排障工作,有效解决无线局域网热点安全问题。展开更多
文摘近年来,赛博物理系统(Cyber-Physical Systems,CPS)随着工业4.0、智能制造的提出而成为行业热点话题,多CPS系统的智能互联是构成当今物联网(The Internet of Things&Services,IOTS)的基础。重点探索多个CPS之间的协同机制,提出了基于总线型智能探头的多CPS互联互通模型以完成多CPS间的通信。在中航工业成飞数控加工厂建立了一套总线型CPS模型,实现了核心协议设计及编码设计,通过车间内各个MES子系统能够充分实现互联互通,以证明设计方法的可行性。
文摘即时检测(Point of Care)技术在快速、灵敏、特异的检测疾病相关的生物因子中得到越来越多的关注。尤其是新兴的即时检测的平台的出现和推广,使得即时检测技术相比传统的诊断技术展现出巨大的优势。稀土上转换材料以其卓越的光学特性,在生物医学领域展现出巨大的潜力,是即时检测材料中冉冉升起的新星。从近年即时检测技术平台、稀土上转换材料等方面介绍了即时检测的发展,详细论述了检测试纸、智能手机和芯片实验室等检测平台、稀土上转换材料在即时检测中的应用。同时结合当前的市场分析,展望了稀土上转换材料在即时检测中未来发展的新趋势。
基金supported by Sino-German research project GZ 1271,Peking Union Medical College(PUMC)Youth Fund(No.3332016056),the Innovation Project of Shandong Academy of Medical Sciences
文摘Nitroreductases(NTRs) are known to be able to metabolize nitro-substituted compounds in the presence of reduced nicotinamide adenine dinucleotide(NADH) as an electron donor. NTRs are present in a wide range of bacterial genera and, to a lesser extent, in eukaryotes hypoxic tumour cells and tumorous tissues, which makes it an appropriate biomarker for an imaging target to detect the hypoxic status of cancer cells and potential bacterial infections. To evaluate the specific activation level of NTR, great efforts have been devoted to the development of fluorescent probes to detect NTR activities using fluorogenic methods to probe its behaviour in a cellular context; however, NTR-responsive MRI contrast agents are still by far underexplored. In this study, para-nitrobenzyl substituted T_1-weighted magnetic resonance imaging(MRI)contrast agent Gd-DOTA-PNB(probe 1) has been designed and explored for the possible detection of NTR.Our experimental results show that probe 1 could serve as an MRI-enhanced contrast agent for monitoring NTR activity. The in vitro response and mechanism of the NTR catalysed reduction of probe 1 have been investigated through LC–MS and MRI. Para-nitrobenzyl substituted probe 1 was catalytically reduced by NTR to the intermediate para-aminobenzyl substituted probe which then underwent a rearrangement elimination reaction to Gd-DOTA, generating the enhanced T1-weighted MR imaging. Further, LC–MS and MRI studies of living Escherichia coli have confirmed the NTR activity detection ability of probe 1 at a cellular level. This method may potentially be used for the diagnosis of bacterial infections.
文摘随着无线局域网(WLAN,Wireless Local Area Networks)用户数据业务量的不断增长,无线局域网维护的工作量也不断增加,严重影响了无线局域网的性能和用户体验和满意度,为建设稳定、高效、安全的无线局域网,这里研究如何实现无线局域网热点智能安全监护系统(WASM,A Smart SecurityMonitoring System of WLAN AP)。首先详细讨论了无线局域网系统的原理和组成,其次讨论使用智能探针方式,实现无线局域网热点远程性能监护和排障工作,有效解决无线局域网热点安全问题。
