1 Introduction Many soda and salt lakes are characterized by the formation of the meromictic conditions under which a part of the water column is not involved in the annual process of mixing(Mac Intyre,Melack,1982).Th...1 Introduction Many soda and salt lakes are characterized by the formation of the meromictic conditions under which a part of the water column is not involved in the annual process of mixing(Mac Intyre,Melack,1982).This creates an展开更多
1 Introduction Prokaryotes are key organisms in aquatic ecosystems as they play animportant role in the biogeochemical cycling of elements.Investigations on the relationships betweenthe diversity of microbial communit...1 Introduction Prokaryotes are key organisms in aquatic ecosystems as they play animportant role in the biogeochemical cycling of elements.Investigations on the relationships betweenthe diversity of microbial community and environmentalfactors offer useful information that bothleads to understanding of the process of element展开更多
基于溶血曼氏杆菌(Mannheimia haemolytica,M.haemolytica)单拷贝基因sodA,通过优化反应条件和体系,建立了溶血曼氏杆菌ddPCR(droplet digital polymerase chain reaction,ddPCR)定量检测方法。结果显示,所建立的溶血曼氏杆菌ddPCR方法...基于溶血曼氏杆菌(Mannheimia haemolytica,M.haemolytica)单拷贝基因sodA,通过优化反应条件和体系,建立了溶血曼氏杆菌ddPCR(droplet digital polymerase chain reaction,ddPCR)定量检测方法。结果显示,所建立的溶血曼氏杆菌ddPCR方法特异性好;检出限(limit of detection,LOD)和定量值(limit of quantification,LOQ)均为170 CFU/m L,3个平行检测的变异系数均小于25%,重复性良好。针对系列稀释的溶血曼氏杆菌纯菌液,ddPCR法能够实现对溶血曼氏杆菌的准确定量,与平板计数结果偏差小于4%。运用所建立的ddPCR方法对15份牛鼻拭子样品进行检测,结果在10份样品中检出溶血曼氏杆菌,含量在3.07×10^(2)~9.52×10^(4)CFU/mL之间。本研究所建立的ddPCR方法适用于临床鼻拭子样品中溶血曼氏杆菌的精准定量分析,可为牛群中溶血曼氏杆菌的监测和治疗提供有效的技术支持。展开更多
文摘1 Introduction Many soda and salt lakes are characterized by the formation of the meromictic conditions under which a part of the water column is not involved in the annual process of mixing(Mac Intyre,Melack,1982).This creates an
基金performed as part of theintegration project of SB RAS № 56
文摘1 Introduction Prokaryotes are key organisms in aquatic ecosystems as they play animportant role in the biogeochemical cycling of elements.Investigations on the relationships betweenthe diversity of microbial community and environmentalfactors offer useful information that bothleads to understanding of the process of element
文摘基于溶血曼氏杆菌(Mannheimia haemolytica,M.haemolytica)单拷贝基因sodA,通过优化反应条件和体系,建立了溶血曼氏杆菌ddPCR(droplet digital polymerase chain reaction,ddPCR)定量检测方法。结果显示,所建立的溶血曼氏杆菌ddPCR方法特异性好;检出限(limit of detection,LOD)和定量值(limit of quantification,LOQ)均为170 CFU/m L,3个平行检测的变异系数均小于25%,重复性良好。针对系列稀释的溶血曼氏杆菌纯菌液,ddPCR法能够实现对溶血曼氏杆菌的准确定量,与平板计数结果偏差小于4%。运用所建立的ddPCR方法对15份牛鼻拭子样品进行检测,结果在10份样品中检出溶血曼氏杆菌,含量在3.07×10^(2)~9.52×10^(4)CFU/mL之间。本研究所建立的ddPCR方法适用于临床鼻拭子样品中溶血曼氏杆菌的精准定量分析,可为牛群中溶血曼氏杆菌的监测和治疗提供有效的技术支持。
