Background:Many researches about in ovo feeding(IOF)of vitamin C(VC)are gradually carried out to explore physiological development in chicken,but little studies focus on VC synthesis capacity of the embryo itself,the ...Background:Many researches about in ovo feeding(IOF)of vitamin C(VC)are gradually carried out to explore physiological development in chicken,but little studies focus on VC synthesis capacity of the embryo itself,the selection of injection site and the effectiveness of IOF of VC.This study aims to explore the above problems.Results:Kidney and yolk sac were the main organs for VC synthesis and L-gulonolactone oxidase(GLO)expression was lower during pre-hatch development than that during post-hatch development.Sodium-dependent vitamin C transporter 1(SVCT1)expression was increased continuously in yolk sac from embryonic age 19(E19)to post-hatch day 1(D1)and in intestine(duodenum,jejunum and ileum)from E17 to D1.Plasma VC content was higher at D1 than that at D21 and D42.IOF of VC significantly reduced GLO expression in liver,kidney and yolk sac as well as SVCT1 expression in duodenum,jejunum and ileum,but increased the VC content in plasma,brain,kidney and liver.In addition,IOF of VC obviously reduced the embryonic morality and increased the hatchability under heat stress.Conclusions:This study suggested that IOF of VC at E11 in yolk was effective for embryonic VC supplementation.These findings provide a theoretical reference about the method of embryonic VC supplementation and effective methodology on embryonic VC nutrition in broiler chickens.展开更多
In ovo feeding of vitamin C(VC)has positive effects on the growth performance,immune and antioxidant function in poultry,which indicates that increasing VC content in eggs may be of benefit.This study was to investiga...In ovo feeding of vitamin C(VC)has positive effects on the growth performance,immune and antioxidant function in poultry,which indicates that increasing VC content in eggs may be of benefit.This study was to investigate the effects of dietary VC supplementation on VC synthesis and transportation and egg deposition.In Exp.1,in order to select a suitable animal model,VC content was detected in different eggs from different layer species.Vitamin C content was lower in ISA Brown breeder eggs and Hy-Line Brown layer eggs(P<0.05)then in Arbor Acres breeder eggs.In Exp.2,a total of 24 Hy-Line Brown layers(42-week-old)were randomly divided into 3 treatments with 8 replicates and fed a basal diet with VC at 0,200 and 400 mg/kg.Sodium-dependent VC transporter 1 and 2(SVCT1 and SVCT2)expressions were higher in ileum than in duodenum and jejunum(P<0.05).SVCT1 expression was higher but SVCT2 expression was lower in the magnum than in the ovary(P<0.05).L-Gulonolactone oxidase(GLO)and SVCT7 expressions were higher but SVCT2 was lower in the kidney than in the liver(P<0.05).Dietary VC supplementation at 400 mg/kg increased SVCT1 expression in duodenum,ovary and magnum,but decreased GLO and SVCT1 expression in liver(P<0.05).Dietary VC supplementation at 200 and 400 mg/kg increased SVCT2 expression in duodenum,but decreased GLO and SVCT1 expression in kidney and SVCT2 expression in liver(P<0.05).Dietary VC supplementation promoted VC absorption in duodenum and jejunum,but reduced endogenous VC synthesis in liver and kidney.Although dietary VC supplementation enhanced VC transportation in ovary and magnum,it did not increase VC deposition in produced eggs.展开更多
维生素C(又名抗坏血酸)是一种基本的微量营养素,作为辅助因子参与多个酶促反应,同时还是一种自由基清除剂。维生素C内稳态主要由两种钠离子依赖的维生素C转运蛋白(sodium-dependent vitamin C transporter,SVCT)——SVCT1和SVCT2来保持...维生素C(又名抗坏血酸)是一种基本的微量营养素,作为辅助因子参与多个酶促反应,同时还是一种自由基清除剂。维生素C内稳态主要由两种钠离子依赖的维生素C转运蛋白(sodium-dependent vitamin C transporter,SVCT)——SVCT1和SVCT2来保持。SVCT1在内皮系统表达,介导了维生素C的肠吸收和肾脏重吸收;而SVCT2表达广泛,表达于脑、骨骼和其他组织,保护这些组织免遭氧化损伤。SVCT的遗传多态性与癌症的发生密切相关。对SVCT介导的维生素C内稳态的保持机制的研究,可使维生素C更好地应用于临床。展开更多
基金funded by the National Key Research and Development Program of China(2017YFD0500500,20170502200)the National Natural Science Foundation of China(No.31972529)+1 种基金the Innovative Transforming Project of Shaanxi Agricultural Achievements in Science&Technology(NYKJ-2018-YL15)the Program for Shaanxi Science&Technology from Shaanxi Provincial Science and Technology Department(2021TD-30).
文摘Background:Many researches about in ovo feeding(IOF)of vitamin C(VC)are gradually carried out to explore physiological development in chicken,but little studies focus on VC synthesis capacity of the embryo itself,the selection of injection site and the effectiveness of IOF of VC.This study aims to explore the above problems.Results:Kidney and yolk sac were the main organs for VC synthesis and L-gulonolactone oxidase(GLO)expression was lower during pre-hatch development than that during post-hatch development.Sodium-dependent vitamin C transporter 1(SVCT1)expression was increased continuously in yolk sac from embryonic age 19(E19)to post-hatch day 1(D1)and in intestine(duodenum,jejunum and ileum)from E17 to D1.Plasma VC content was higher at D1 than that at D21 and D42.IOF of VC significantly reduced GLO expression in liver,kidney and yolk sac as well as SVCT1 expression in duodenum,jejunum and ileum,but increased the VC content in plasma,brain,kidney and liver.In addition,IOF of VC obviously reduced the embryonic morality and increased the hatchability under heat stress.Conclusions:This study suggested that IOF of VC at E11 in yolk was effective for embryonic VC supplementation.These findings provide a theoretical reference about the method of embryonic VC supplementation and effective methodology on embryonic VC nutrition in broiler chickens.
基金funded by the National Key Research and Development Program of China(2017YFD0500500,20170502200)the National Natural Science Foundation of China(No.31972529)+1 种基金the Program for Shaanxi Science&Technology(2017TSCXLNY-04-04,2018ZDCXL-NY-0201,2018ZDXM-NY-051)the Program for Yangling Agricultural High-tech Industries Demonstration Zone(2018CXY-10)。
文摘In ovo feeding of vitamin C(VC)has positive effects on the growth performance,immune and antioxidant function in poultry,which indicates that increasing VC content in eggs may be of benefit.This study was to investigate the effects of dietary VC supplementation on VC synthesis and transportation and egg deposition.In Exp.1,in order to select a suitable animal model,VC content was detected in different eggs from different layer species.Vitamin C content was lower in ISA Brown breeder eggs and Hy-Line Brown layer eggs(P<0.05)then in Arbor Acres breeder eggs.In Exp.2,a total of 24 Hy-Line Brown layers(42-week-old)were randomly divided into 3 treatments with 8 replicates and fed a basal diet with VC at 0,200 and 400 mg/kg.Sodium-dependent VC transporter 1 and 2(SVCT1 and SVCT2)expressions were higher in ileum than in duodenum and jejunum(P<0.05).SVCT1 expression was higher but SVCT2 expression was lower in the magnum than in the ovary(P<0.05).L-Gulonolactone oxidase(GLO)and SVCT7 expressions were higher but SVCT2 was lower in the kidney than in the liver(P<0.05).Dietary VC supplementation at 400 mg/kg increased SVCT1 expression in duodenum,ovary and magnum,but decreased GLO and SVCT1 expression in liver(P<0.05).Dietary VC supplementation at 200 and 400 mg/kg increased SVCT2 expression in duodenum,but decreased GLO and SVCT1 expression in kidney and SVCT2 expression in liver(P<0.05).Dietary VC supplementation promoted VC absorption in duodenum and jejunum,but reduced endogenous VC synthesis in liver and kidney.Although dietary VC supplementation enhanced VC transportation in ovary and magnum,it did not increase VC deposition in produced eggs.
文摘维生素C(又名抗坏血酸)是一种基本的微量营养素,作为辅助因子参与多个酶促反应,同时还是一种自由基清除剂。维生素C内稳态主要由两种钠离子依赖的维生素C转运蛋白(sodium-dependent vitamin C transporter,SVCT)——SVCT1和SVCT2来保持。SVCT1在内皮系统表达,介导了维生素C的肠吸收和肾脏重吸收;而SVCT2表达广泛,表达于脑、骨骼和其他组织,保护这些组织免遭氧化损伤。SVCT的遗传多态性与癌症的发生密切相关。对SVCT介导的维生素C内稳态的保持机制的研究,可使维生素C更好地应用于临床。
