Differential Expression of Genes Related to Fruit Development and Capsaicinoids Synthesis in Habanero Pepper Plants Grown in Contrasting Soil Types

Habanero pepper(Capsicum chinense Jacq.)is a crop of economic relevance in the Peninsula of Yucatan.Its fruits have a high level of capsaicinoids compared to peppers grown in other regions of the world,which gives them industrial importance.Soil is an important factor that affects pepper development,nutritional quality,and capsaicinoid content.However,the effect of soil type on fruit development and capsaicinoid metabolism has been little understood.This work aimed to compare the effect of soils with contrasting characteristics,black soil(BS)and red soil(RS),on the expression of genes related to the development of fruits,and capsaicinoid synthesis using a transcriptomic analysis of the habanero pepper fruits.Plants growing in RS had bigger fruits and higher expression of genes related to floral development,fruit abscission,and softening which suggests that RS stimulates fruit development from early stages until maturation stages.Fruits from plants growing in BS had enrichment in metabolic pathways related to growth,sugars,and photosynthesis.Besides,these fruits had higher capsaicinoid accumulation at 25 days post-anthesis,and higher expression of genes related to the branched-chain amino acids metabolism(ketol-acid reductisomerase KARI),pentose phosphate pathway and production of NADPH(glucose-6-phosphate-1-dehydrogenase G6PDH),and proteasome and vesicular traffic in cells(26S proteasome regulatory subunit T4 RPT4),which suggest that BS is better in the early stimulation of pathways related to the nutritional quality and capsaicinoid metabolism in the fruits.

Soil Organic Carbon Stock Variation under Different Soil Types and Land Uses in the Sub-Humid Noun Plain, Western Cameroon

This study was conducted to assess the current stock of soil organic carbon under different agricultural land uses, soil types and soil depths in the Noun plain in western Cameroon. Three sites were selected for the study, namely Mangoum, Makeka and Fossang, representative of the three dominant soil types of the noun plain (Andosols, Acrisols and Ferralsols). Three land uses were selected per site including natural vegetation, agroforest and crop field. Soil was sampled at three depths;0 - 20 cm, 20 - 40 cm, and 40 - 60 cm. Analysis of variance showed that soil type did not significantly influence carbon storage, but rather land uses and soil depth. SOCS decreased significantly with depth in all the sites, with an average stock of 66.3 ± 15.8 tC/ha at 0 - 20 cm, compared to an average stock of 33.3 ± 7.4 tC/ha at 40 - 60 cm. SOCS was significantly highest in the natural formation with 57.2 ± 19.7 tC/ha, and lowest in cultivated fields, at 37.7 ± 10.6 tC/ha. Andosols, with their high content of coarse fragments, stored less organic carbon than Ferralsols and Acrisols.

Effects of Crops, Soil Types and Irrigation Methods on Digestion Capacity of Biogas Slurry

In the research, pot experiment and field testing were conducted to study the effect of different crops, soil types, and irrigation modes on biogas slurry diges- tions. The results showed that when silage maize, sweet sorghum and Chinese cabbage were planted in purple soils, the quantities of digested biogas slurry were of 57, 157.5, and 34.5 t/hm2, respectively, while the quantities of digested biogas slurry were 70.5, 157.5 and 40.5 t/hm2 in yellow soils. Besides, the digested biogas slurries reached 36 and 27 t/hm2 as per flood irrigation and sprinkling irrigation when Chinese cabbages were planted in yellow soils. The research indicated crop variety, soil type, and irrigation method all have effects on farmland digestion of biogas slurry.

Comparison of Soil Organic Carbon Content in Different Soil Types of Wuyi Mountains

[Objective] The aim was to explore the soil organic carbon（SOC）content from different types of soil and its influencing factors.[Method] Soil samples were collected from Wuyi Mountains,and the physical and chemical properties were measured and the relationship between SOC content and soil physical and chemical properties,altitude,average annual temperature was analyzed.[Result] SOC content ranged from 14.91 to 112.34 g/kg,and SOC content was significant positive correlated with available nutrients（available N,P and K）and total nitrogen.[Conclusion] This study indicated that the physical and chemical properties of soil,altitude,average annual temperature had a significant influence on the SOC content of Wuyi Mountains.

Influence of water potential and soil type on conventional japonica super rice yield and soil enzyme activities

We carried out a pool culture experiment to determine the optimal water treatment depth in loam and clay soils during the late growth stage of super rice. Three controlled water depth treatments of 0-5, 0-10 and 0-15 cm below the soil surface were established using alternate wetting and drying irrigation, and the soil water potential （0 to -25 kPa） was measured at 5, 10 and 15 cm. A 2-cm water layer was used as the control. We measured soil enzyme activities, root antioxidant enzyme activities, chlorophyll fluorescence parameters, and rice yield. The results showed that the 0-5-cm water depth treatment significantly increased root antioxidant enzyme activities in loam soil compared with the control, whereas soil enzyme activities, chlorophyll fluorescence parameters and yield did not differ from those of the control. The 0-10- and 0-15-cm water depth treatments also increased root antioxidant enzyme activities, whereas soil enzyme activities, chlorophyll fluorescence parameters and yield decreased. In clay soil, the soil enzyme activities, root antioxidant enzyme activities, chlorophyll fluorescence parameters, and yield did not change with the 0-5-cm water treatment, whereas the 0-10- and 0-15-cm water treatments improved these parameters. Therefore,the appropriate depths for soil water during the late growth period of rice with a 0 to -25 kPa water potential were 5 cm in loam and 15 cm in clay soil.

Mapping Detailed Soil Property Using Small Scale Soil Type Maps and Sparse Typical Samples

Soil type maps at the scale of I Z 1 000 000 are used extensively to provide soil spatial distribution information for soil erosion assessment and watershed management models in China. However, the soil property maps produced through conventional direct linking method usually suffer low accuracy as well as the lack of spatial details within a soil type polygon. This paper presents an effective method to produce detailed soil property map based on representative samples which were extracted from each polygon on the 1 ： 1 000 000 soil type map. The representative sample of each polygon is defined as the location that can represent the largest area within the polygon. The representativeness of a candidate sample is determined by calculating the soil-forming environment condition similarities between the sample and other locations. Once the representative sample of each polygon has been chosen, the property values of the existing typical samples are assigned to the corresponding representative samples with the same soil type. Finally, based on these representative samples, the detailed soil property map could be produced by using existing digital soil mapping methods. The case study in XuanCheng City, Anhui Province of China, demonstrated the proposed method could produce soil property map at a higher level of spatial details and accuracy： 1） The soil organic matter （SOM） map produced based on the representative samples can not only depict the detailed spatial distribution of SOM within a soil type polygon but also largely reduce the abrupt change of soil property at the boundaries of two adjacent polygons. 2） The Root Mean Squared Error （RMSE） of the SOM map based on the representative samples is 1.61, and it is 1.37 for the SOM map produced by using conventional direct linking method. Therefore, the proposed method is an effective approach to produce spatial detailed soil property map with higher accuracy for environment simulation models.

Spatial patterns of insect herbivory within a forest landscape:the role of soil type and forest stratum

Background:Insect herbivory has profound impacts on ecosystem processes and services.Although many efforts have been made to recognize the main drivers of insect herbivory at different scales,the results are inconsistent.One likely reason is that studies have insufficiently captured the spatially heterogeneous factors such as soil type and forest stratum within the stand that may significantly affect insect herbivory.In particular,there is a lack of studies that address the detailed spatial patterns of insect herbivory which are influenced by these factors.Methods:We measured the detailed spatial patterns of insect herbivory on cork oak(Quercus variabilis Bl.)in response to soil type(gravel soil and loam)and forest stratum(the upper,lower,and sapling stratum),and correlated these patterns with a set of influencing factors(litter coverage,coverage of shrubs and herbs,soil nutrients,soil moisture,and leaf traits)in a forest landscape.Results:Generally,insect herbivory was spatially heterogeneous within stands.Herbivory was significantly lower in gravel soil areas than in loam soil areas and the highest herbivory occurred in the lower stratum.However,there were also 41 individual plots in which the highest herbivory occurred in the upper stratum and 29 plots in which the highest herbivory occurred in the sapling stratum.There were significant differences in soil nutrient and water status between soil types,but no significant differences in leaf traits.The effects of forest stratum on leaf traits were also inconsistent with those on insect herbivory.Conclusions:Leaf traits may not be the main factors influencing insect herbivory in the field.Soil type may have major effects on herbivory patterns by influencing litter coverage while higher coverage of shrubs and herbs may reduce herbivory in the sapling stratum.These findings may advance our understanding of tree-herbivore interactions in real-world situations and have important implications for the sustainable management of forest ecosystems.

Available Selenium in Main Soil Types of China and ItsRelation to Some Soil Properties

Forty-eight soil profiles were sampled from different ecological and pedogeochemical areas of China and their available selenium (Av-Se) contents were determined. Results showed that the content of Av-Se in the profiles of soils in China ranged within 2.49-18.10 μg kg--1 ) with a mean value of 10.01 μg kg--1，and was in the sequence of Ferralisols > Luvisols > Isohumisols > Aridisols. The correlation analysis between Av-Se and the soil physical-chemical properties revealed that the correlations between Av-Se and O.M., CEC, Fe2O3,Al2O3, ignition loss, Co and Zn were positive at a highly significant level, but those between Av-Se and pH,base saturation, CaO, Ba and Sr were all negative at a highly significant level. The reason of some diseases related to the Se deficiency might be the leaching loss of selenium in some soils.

Impact of Soil Type Used in Tunneling on Land Subsidence and Mobility Effective Time under Different Earthquake Records

During recent decades, tunnels construction in urban environments has been recognized as a useful factor in improving transportation situation in cities. Construction of underground structures like tunnels has a direct impact on the above-ground structures. The most important impacts are soil crust movement of the structure movement), acceleration change of the earthquake on structures, and land subsidence. The present research aims at seismic evaluation of the effect of soil type in land subsidence and effective mobility time in tunneling projects. For this purpose, two different soil types are chosen to model and assess factors using numerical methods. The outcome of this research concludes that tunneling changes in the dominant frequency record if these changes reach to the extent that structure frequency equals to record frequency, resonance phenomenon happens.

REGIONAL DIFFERENTIATION OF CRITICAL CONCENTRATION OF ARSENIC(As) AND CADMIUM (Cd) IN MAIN SOIL TYPES OF CHINA

Studies of critical concentration distribution features of polluted elements of Cd and As in main soil types of China, such as laterite, crimson soil, purplish soil, yellow brown soil, brunisolic soil, black soil, cinnamon soil and sierozem revealed that soil types vary in different zones, so do their critical concentrations.The critical concentration has a zonal differentiation with the distribution of soil types from south to north, and from east to west. The critical concentration of Cd increases from south to north (yellow brown soil,brunisolic soil and cinnamon soil),and from east to west (black soil and sierozem). This coincides With changes of climate zones and soil zones. On the contrary, the critical concentration of Asdecreases from south to north and from east to west.

THE INFLUENCE OF FERTILIZERS, NaHCO_3,SOIL TYPE ON CHEMISTRY OF FLOODED SOILS AND RICE PLANT GROWTH

This research was conducted to study the influence of NaHCO3and fertilizer treatments on flooded soils tothe chemistry of soil and rice plant growth.The experiment was done in a greenhouse using a split-split plot design ofthree factors: two types of soil,three doses of fertilizers and three levels of NaHCO3with three replications.The firstfactor was two kind of soils Fluvaquent and Calciquert,the factor was without nutrient(blank),macro nutrients,andcompletely nutrients(second macro and micro nutrients),and the third factor was 0 mg/kg,200 mg/kg and 400 mg/kgof NaHCO3or equivalent with 0 mg,1 200 mg and 2 400 mg NaHCO3per pot.Each experimental unit used a pot of 10L capacity and filled with soil mud equivalent with 6 kg of dried soil.After each of treatment combination mixed into themud soil,two plants of IR-32 variety were grown,and flooded at5 cm high accordingly with growth period.Parametersobserved were the growth of tillers,electrical conductivity(E.C.) and pH.The experiment results showed that the treatment of NaHCO3increased the soluble salt content(E.C.) and soilacidity(pH) during flooding on Calciquerts and Fluvaquents.The content of soluble salt decreased to 2.0 mmhos/cm,while pHon Calciquert decreased towards the neutral pH.Anyhowthe pHon Fluvaquents increased approach to neutral.The treatments of NaHCO3inhibited the tiller growth,either macro fertilizer and completely fertilizer increased thetillers,but did not improve the negative effect of alkalinity due to of NaHCOtreatment.

Effects of Soil Types and Moisture Contents on Emergence Rate of Contarinia morulae Jiang

In order to explore the relationship between soil moisture content and the severity of occurrence of Contarinia morulae Jiang,the effects of different soil types and moisture contents on the emergence rate of C. morulae were investigated. The results showed that soil moisture contents had a significant effect on the emergence rate of C. morulae. When the soil moisture content was 10%,the emergence rate of C. morulae was the highest in both soil types. When the soil moisture content was too high or too low,the emergence rate of C. morulae was significantly reduced. Within the suitable water content range,the emergence rate of C. morulae in sandy soil was significantly higher than that in sandy loam soil. The trend of emergence of C. morulae in the two soil types was basically the same. The number of adults of C. morulae began to increase slowly on March 18,peaked on March 28 and then peaked again on early April.

Distribution of antibiotic resistant bacteria in different soil types following manure application

ARB was investigated in different soil types following manure application.CTC-manure induced more resistance of soil indigenous microbes in fluvo-aquic soil.Lactobacillus,Dyella,Ralstonia,and Bacillus were the key different genera.Manure control is an effective way to reduce the risk of soil ARB.

PLFA Analysis of Soil Microbial Community Structure in Different Forest Types

Soil soluble organic matter is an important component in the study of carbon and nitrogen cycling in terrestrial ecosystems. Soil microorganisms, as soil decomposers, participate in soil biogeochemical processes and play an important role in maintaining the balance of soil ecosystems. As a typical subtropical regional unit, Queensland, Australia, is a relatively concentrated distribution area of forests in Australia. It is very sensitive to climate change and plays an important role in Australian climate and even global climate change. Its unique natural environment and ecosystem occupy a special position in the world. However, the knowledge of available carbon and nitrogen pool and microbial activity in forest soil is still very limited. Pinus elliottii, Araucaria cunninghamii and Agathis australis are the three most important forest types in southern Queensland, Australia. In our research, the function and structural diversity of soil microbial communities of these three forest types were studied using biochemical and molecular biological methods, and the effective carbon and nitrogen pools of soil of different forest types and related microbial processes were discussed, which has important theoretical guiding significance for further research on the structure and function of soil ecosystem. The number of PLFAs in the soil of P. elliottii was 45, the number of PLFAs in the soil of Araucaria cunninghamii and Agathis australis was 39 and 35, respectively. The number and content of PLFAs monomer in P. elliottii were higher than those in the other two kinds of forest soil.

Effects of self-healing biomimetic subsoiler on tillage resistance, wearcorrosion performance and soil disturbance morphology under different soil types

Subsoiling has been widely used all over the world as an important operation method of no-tillage farming.For energy-saving and life-extension,the tillage resistance and wear-corrosion of subsoilers have attracted wide attention.In this study,the tillage resistance,soil disturbance,wear and corrosion of subsoiler with S-T-SK-2#biomimetic structures(S means subsoiler;T means tine;SK means shank;2#,h/s=0.57,h=5 mm andα=45°.)and self-healing coating under two seasons,two locations with different soil properties(black loam and clay soil)and subsoiling speeds(2 km/h and 3.6 km/h)were investigated.The soil moisture content and compactness affected the tillage resistance and wear-corrosion.The tillage resistance and degree of corrosion on all subsoilers were much larger in clay soil than that in black loam soil.Compared with S-T-SK-2#,the tillage reduction rate of C-S-T-SK-2#(S-T-SK-2#with self-healing coating)was up to 14.32%in clay soil under the speed of 2 km/h.The significance tests of regression equation results showed that subsoiler type and soil properties had a significant impact on soil disturbance coefficient,swelling of total soil layer,bulkiness of the plough pan.It is of a guiding significance for the analysis of soil disturbance.Synergism mechanism of subsoiler coupling with biomimetic structures and self-healing coating was analyzed in following.It depicted the guiding effect of biomimetic structure and the shield function of self-healing coating,resulting in anticorrosion and wear resistance of subsoiler.

Effects of Typical Soil and Stratification Thickness on Water Infiltration Characteristics in Central Ningxia

In order to compare the influence of different soil types and stratification on water infiltration capacity,two main types of soil in the desert steppe,sierozem(S)and aeolian sandy soil(A),were selected,and infiltration simulation tests were conducted on homogeneous soil and layered soil(layer thickness 5,10,and 20 cm),respectively.The results show that during the whole experiment,there was a small difference between S5A95(aeolian sandy soil 95 cm thick was covered with sierozem 5 cm thick)and S10A90(aeolian sandy soil 90 cm thick was covered with sierozem 10 cm thick)in the wetting front process,infiltration rate and cumulative infiltration,but there was a significant difference between S5A95 and S20A80(aeolian sandy soil 80 cm thick was covered with sierozem 20 cm thick).In the initial infiltration stage,there was no significant difference between A5S95(sierozem 95 cm thick was covered with aeolian sandy soil 5 cm thick)and A10S90(sierozem 90 cm thick was covered with aeolian sandy soil 10 cm thick).However,with the increase of infiltration time,the wetting front process,A5S95,A10S90 and A20S80 had significant differences in terms of wetting front process,infiltration rate and cumulative infiltration.The infiltration capacity of A was significantly higher than that of S.Combined with linear R 2 value and model parameters,the three infiltration models were comprehensively compared,and the fitting process and results of the general empirical model for the infiltration process of homogeneous soil and layered soil showed good results.Three models were used to simulate the water infiltration process of layered soil with different textures,and the order of the effect is as follows:general empirical model>Kostiakov model>Philip model.Soil type and layer thickness had a great influence on water infiltration process.When sierozem was covered with aeolian sandy soil 20 cm thick,the infiltration capacity was the best.As aeolian sandy soil was covered with sierozem 10 cm thick,the infiltration effect was the worst.Therefore,once coarse graying occurs on the surface of sierozem(the thickness of sand is more than 20 cm)or when the content of fine particles overlying aeolian sandy soil(the thickness of silt and clay soil is more than 10 cm)during ecological restoration is high,the soil hydrological characteristics will change significantly,which may lead to changes in vegetation types and even ecosystem structure.

Factors Influencing the Acidification Trends in Agriculture Soils: A Case Study of Slovakia

This study aims to evaluate the development of soil reaction values in 15 key localities of soil Partial Monitoring System from 1994 to 2023, and to identify the most important regional drivers of pH value development. Soil samples were collected from the depth 0 - 0.10 m yearly in the spring (5 samples from each locality). In the dry soil, samples were determined actively and exchanged soil reaction. The most significant negative changes (decreases of soil reaction) were determined in Haplic Stagnosols group and Cambisols group. The pH value in topsoil is primarily controlled by soil type and soil substrate, soil management and land use, and to a lesser extent by climatic region.

Study on strength properties and soil behaviour type classification of Huanghe River Delta silts based on variable rate piezocone penetration test

Fine-grained silt is widely distributed in the Huanghe River Delta(HRD)in China,and the sedimentary structure is complex,meaning that the clay content in the silt is variable.The piezocone penetration test(CPTu)is the most widely approved in situ test method.It can be used to invert soil properties and interpret soil behavior.To analyse the strength properties of surface sediments in the HRD,this paper evaluated the friction angle and its inversion formula through the CPTu penetration test and monotonic simple shear test and other soil unit experiments.The evaluation showed that the empirical formula proposed by Kulhawy and Mayne had better prediction and inversion effect.The HRD silts with clay contents of 9.2%,21.4%and 30.3%were selected as samples for the CPTu variable rate penetration test.The results show as follows.(1)The effects of the clay content on the tip resistance and the pore pressure of silt under different penetration rates were summarized.The tip resistance Q_t is strongly dependent on the clay content of the silt,the B_(q)value of the silt tends to 0 and is not significantly affected by the change of the CPTu penetration rate.(2)Five soil behavior type classification charts and three soil behavior type indexes based on CPTu data were evaluated.The results show that the soil behavior type classification chart based on soil behavior type index ISBT,the Robertson 2010 behavior type classification chart are more suitable for the silty soil in the HRD.

Analysis and Assessment on the Heavy Metals in a Severely Degraded Subtropical Red Soil Region

5 different forests of Pinus massoniana, Schima superba, Liquidambar formosana, P. massoniana × S. superba, P. massoniana × L. formosana as the research object were set up to study the Cr, Cu and Zn content of degraded red soil region in subtropics. The soil heavy metal pollution degree was evaluated by national environmental quality standard (II class). The results showed that three soil metals of P. massoniana × S. superba were the highest, and the soil metals enrichment ability was strong. The order of single factor pollution index of metal elements was Cu (1.38) > Cr (0.81) > Zn (0.42), and moderately pollution, pollution warning and no pollution, respectively. There was no significant correlation between three soil heavy metals and soil total carbon (TC), total nitrogen (TN) and total phosphorus (TP). These results suggested that the accumulation of heavy metal elements was not derived from the parent material of soil. There was a significant positive correlation between the three metal elements which indicated that the sources of the three elements were similar. The structural equation model showed that the direct and indirect effects among the influencing factors ultimately affected the activity of heavy metals by cascade effects.

Soil type-dependent effects of drying-wetting sequences on aggregates and their associated OC and N

Evaluating the impacts of drying-wetting(DW)cycles on soil aggregates and their associated organic carbon(OC)and nitrogen(N)is crucial to understand the OC and N cycles.Soils are likely subjected to DW cycles with different sequences depending on seasons or in agroecosystems.However,studies on how DW sequences influence OC and N dynamics within aggregates,and whether this effect is dependent on soil type,are relatively limited.Herein,two DW sequences,i.e.,drying-wetting-dryingwetting(2DW)and wetting-drying-wetting-drying(2WD)treatments were designed,and a consistent wetting(CW)was set as a control to assess the effects of DW sequences.Four soils(Entisol,Ultisol,Anthrosol and Mollisol)varying in texture and OC content were used.The aggregate size distribution,the OC,total N(TN),readily oxidizable OC(ROOC)and mineral N(Min-N)content in aggregates were determined.Results showed that 2DW treatment increased but 2WD treatment decreased the large aggregates of Entisol and Ultisol,while 2DW and 2WD treatments synchronously increased the large aggregates of Anthrosol but decreased them of Mollisol.Two DW treatments increased the OC in each aggregate of Entisol,Anthrosol and Mollisol but decreased them of Ultisol.The 2DW didn't affect but 2WD treatment decreased ROOC in 1e2 mm aggregates of Entisol and<0.25 mm aggregates of Ultisol and Mollisol.The 2DW and 2WD treatments minimally affected TN but potentially influenced Min-N in aggregates.The 2DW and 2WD treatments both decreased the Min-N in each aggregate size class of Entisol and Mollisol,while 2DW increased but 2WD treatment decreased Min-N in each aggregate of Ultisol and Anthrosol.These results indicated that the varied effects of DW sequences and the interactive effects of soil type with DW sequences on aggregate turnover and OC and N cycling should be reconsidered to provide more precisive evidences for global C and N cycles under the scenario of future climate changes.