As a macronutrient,Phosphorus(P)takes many roles in plant growth and development.It should be significant to explore the molecular mechanism of low-phosphorus stress response of plants.Phosphate starvation response(PH...As a macronutrient,Phosphorus(P)takes many roles in plant growth and development.It should be significant to explore the molecular mechanism of low-phosphorus stress response of plants.Phosphate starvation response(PHR)transcription factors play important roles in response to phosphorus deficiency stress in plants.In this study,we isolated a gene related to the plant phosphorus signaling system from the acid-soil-resistant centipedegrass(Eremochloa ophiuroides[Munro]Hack.),termed EoPHR2.The subcellular localization of EoPHR2 protein was observed to be nuclear located.The expression patterns of EoPHR2 in different tissues and Al/Pi-stress conditions were analyzed by qRT-PCR,they suggested a potential role in response to the multiple-stress under acid soil adversity.Based on the functional identification through transgenic plants,we found that(1)EoPHR2 is involved in the Pi-signaling pathway,and(2)overexpression of EoPHR2 mimics Pi-starvation signalling resulting on enhanced roots whether under Pi-deficiency stress or not.In conclusion,EoPHR2 transcription factor plays a role in response to the multiple stresses under acid soil conditions,improving the low-phosphorus stress resistance of Eremochloa ophiuroides.展开更多
基金This work was supported by the National Natural Science Foundation of China(Grant No.31902046)the National Natural Science Foundation of China(Grant No.32072608)+1 种基金the National Natural Science Foundation of China(Grant No.31902060)the Natural Science Foundation of Jiangsu Province,China(Grant No.BK20180315),and the Foundation of Jiangsu Key Laboratory for the Research and Utilization of Plant Resources(Institute of Botany,Jiangsu Province and Chinese Academy of Sciences).
文摘As a macronutrient,Phosphorus(P)takes many roles in plant growth and development.It should be significant to explore the molecular mechanism of low-phosphorus stress response of plants.Phosphate starvation response(PHR)transcription factors play important roles in response to phosphorus deficiency stress in plants.In this study,we isolated a gene related to the plant phosphorus signaling system from the acid-soil-resistant centipedegrass(Eremochloa ophiuroides[Munro]Hack.),termed EoPHR2.The subcellular localization of EoPHR2 protein was observed to be nuclear located.The expression patterns of EoPHR2 in different tissues and Al/Pi-stress conditions were analyzed by qRT-PCR,they suggested a potential role in response to the multiple-stress under acid soil adversity.Based on the functional identification through transgenic plants,we found that(1)EoPHR2 is involved in the Pi-signaling pathway,and(2)overexpression of EoPHR2 mimics Pi-starvation signalling resulting on enhanced roots whether under Pi-deficiency stress or not.In conclusion,EoPHR2 transcription factor plays a role in response to the multiple stresses under acid soil conditions,improving the low-phosphorus stress resistance of Eremochloa ophiuroides.
