Studies on Membrane Function and Sugar Components of Ultradried Seeds

The effects of ultradry storage on the activity of ATPase and fluidity of plasma membrane in Chinese cabbage (Brassica pekinensis (Lour.) Rupr.) and elm ( Ulmus pumila L.) seeds were investigated. The results indicated that no significant differences in the activity of ATPase and the micro- viscosity of plasma membrane of ultradried (UD) seeds could be found as compared to the control seeds stored under -20 degreesC, although there was a little adverse effect on the seeds with extreme dehydration. The results were consistent with higher vigor level of UD seeds. This implied that ultradry seed storage could protect the integrity of the membrane, maintain its physiological function and improve the storability of seeds. The relationship between sugar and desiccation tolerance of UD seeds was analysed using the HPLC. The results showed that the ratio of reducing to nonreducing sugar was lower in UD seeds than that in control. The content of sucrose and stachyose in elm and Chinese cabbage UD seeds was related to their desiccation tolerance, while no stachyose was detected in corn (Zea mays L.) seeds. This could be one of the reasons for its sensitivity to desiccation.

MIR1868 negatively regulates rice cold tolerance at both the seedling and booting stages

Low temperature causes rice yield losses of up to 30%–40%,therefore increasing its cold tolerance is a breeding target.Few genes in rice are reported to confer cold tolerance at both the vegetative and reproductive stages.This study revealed a rice-specific 24-nt miRNA,miR1868,whose accumulation was suppressed by cold stress.Knockdown of MIR1868 increased seedling survival,pollen fertility,seed setting,and grain yield under cold stress,whereas its overexpression conferred the opposite phenotype.Knockdown of MIR1868 increased reactive oxygen species(ROS)scavenging and soluble sugar content under cold stress by increasing the expression of peroxidase genes and sugar metabolism genes,and its overexpression produced the opposite effect.Thus,MIR1868 negatively regulated rice cold tolerance via ROS scavenging and sugar accumulation.

Effect of Sugar on the Process of Cold-Acclimation-Induced Freezing Tolerance of Populus tomentosa

Populus tomentosa seedlings for cold-acclimating were pretreated wit h or without 20% saccharose. Changes in the concentrations of total soluble sugar , the survival rates, and freezing tolerance of seedlings during cold acclimatio n were investigated. The results showed that cold acclimation increased the conc entrations of total soluble sugar, the survival rates and freezing tolerance. Co ld acclimation, combined with the saccharose-pretreatment, enhanced the above- ment ioned effect of cold acclimation, and obviously increased the concentrations of total soluble sugar, the survival rates and freezing tolerance of seedlings. Fur ther analysis found that the concentrations of total soluble sugar in branches i ncreased greater than that in leaves during both cold acclimation with or withou t the pretreatment of saccharose. Moreover, an increase of the concentrations of total soluble sugar in branches and leaves was closely related to the freezing tolerance of seedlings. The results indicate the accumulation of soluble sugar i n seedlings induced by cold acclimation may be involved in the induction of free zing tolerance .

Effects of Different Light Qualities on the Growth Characteristics of Populus trinervis

Populus trinervis is native to China and plays an irreplaceable role in maintaining the ecological balance of boreal and temperate forests.P.trinervis mainly grows in high-altitude areas.At present,there are limited studies on the response of P.trinervis to different light qualities,so it is necessary to investigate the photosynthetic physiological changes of P.trinervis in different light environments.In our study,P.trinervis was grown for 8 months under light filtered by three different colored films.The three treatments were blue film,green film,and white plastic film.The effects of blue(B),green(G),and white(W)light on photosynthetic pigment content,absolute growth,photosynthetic parameters,soluble sugar content,and chlorophyll fluorescence parameters were studied,respectively.Compared to the Wtreatment,the chlorophyll a and b,carotenoids,total chlorophyll content(a+b),absolute growth of seedling height,net photosynthetic rate(PN),water use efficiency(WUE),total soluble sugars,sucrose,and nonphotochemical quenching(NPQ)of P.trinervis were significantly increased under B treatment.Meanwhile,chlorophyll a and b,carotenoids,total chlorophyll(a+b),transpiration rate(Tr),intercellular CO_(2) concentration(Ci),stomatal conductance(gs),absolute growth of seedling height and leaf length,reducing sugar,total soluble sugar content,and NPQ were significantly increased under G treatment.The results showed that the absolute growth and chlorophyll content of P.trinervis were increased under B light,while the sugar and photosynthetic parameters were increased under G light.Additional studies may look into how B light impacts absolute growth and promotional mechanisms,as well as how G light affects the accumulation of sugar levels.

Effect of Foliar Fertilizer Spraying at Seedling Stage on the Growth Physiological Characteristics and Yield of Brassica napus L.

In order to select a suitable foliar fertilizer for Brassica napus L.at the seedling stage,using‘Fengyou 958’as the material,different foliar fertilizers including BR,Se,Si,BR+Si,BR+Se,Se+Si and BR+Se+Si were sprayed at the seedling stage to study their effect on the physiological characteristics,growth and yield of Brassica napus L..The results showed that the growth,chlorophyll content,soluble sugar content,soluble protein content,and yield of different treatments at the budding stage improved compared with the control.The effect of the Si+Se treatment was the best,followed by the Se and Si treatments.The chlorophyll content of the flower decreased continuously during the whole flowering period,and the chlorophyll content of the mature silique peel was higher than that of the seed.The content of soluble sugar in flowers was the highest in the early flowering stage,and the content of soluble sugar in leaves at the flowering stage was higher than that at the budding stage.The soluble sugar content in the harvested silique peel decreased gradually with the increase of time,and that in the silique peel and seed was similar at 35 d after pollination.The soluble protein content in the silique peel and seed decreased gradually at the mature stage,and the soluble protein content in the silique peel was higher than that in the seed at the same stage.Si+Se foliar fertilizer spraying at the seedling stage can promote the growth and yield of Brassica napus L.and can be applied in Brassica napus L.production.

Effects of Different Nitrogen Fertilizer Rates on Soluble Sugar,Starch and Root Tissue Structure of the Peach Trees

[Objective] The research aimed to provide theoretical basis for the cultivation and production of peach.[Method] The three-year-old seedling peach tree was used as the materials,and NH4NO3 was used as the experimental nitrogen fertilizer.Three nitrogen levels,0,3,6 g per pot respectively were set to study the effects of different nitrogen fertilizer on the contents of soluble sugar,starch and internal structure of the root of the peaches.[Result] The contents of soluble sugar of roots and leaves increased with the increase of nitrogen fertilizer level,and the level of 6 g per pot was the highest,which showed extreme differences between the level of 3 g per pot and the control.However,starch contents showed opposite results,which decreased as the level of nitrogen fertilizer increased.Compared with the 3 g per pot level of nitrogen fertilizer and the control,the internal structure of root applied with nitrogen fertilizer of 6 g per pot level had larger fibrovascularcylinder,advanced vascular bundle,small-spaced thin-walled cells,smooth and full cells with smooth cell wall.[Conclusion]Appropriate nitrogen levels can accelerate the accumulation of soluble sugar and the growth of root tissue structure.

Effect of Alkali Stress on Soluble Sugar, Antioxidant Enzymes and Yield of Oat

Alkali stress can cause severe crop damage and reduce production. However, physiological processes involved in alkali stress in oat seedlings are not well understood. In this study, physiological responses and yield of oat to alkali stress were studied using the alkali-tolerant oat genotype Vao-9 and the alkali-sensitive oat genotype Baiyan 5. The results were： （i） low concentrations of alkali stress （25 and 50 mmol L^-1） significantly reduced the yield and grain weight while increased the oat grain number per spike. A negative correlation between yield and malondialdehyde （MDA） content at the jointing and grain filling stages and positive correlations between yield on one hand and superoxide dismutase （SOD）, and peroxidase （POD） activities on the other at the jointing stage were observed. There was a positive correlation between MDA and soluble sugar at the grain filling stage; （ii） soluble sugar content was increased at the jointing and grain filling stages and decreased at the heading stage by alkali stress; （iii） alkali stress increased the SOD activity during the heading and grain filling stages, and increased the POD activity at the heading stage. As compared to the control, the increase of MDA contents in alkali-treated oat was observed, during the jointing, heading and grain filling stages; （iv） under alkali stress, the oat genotype Vao-9 showed higher antioxidant enzyme activity and lower soluble sugar contents during the heading stage, and lower MDA contents than those in the oat genotype Baiyan 5 under alkali stress. The result suggested that the high ROS scavenging capacity and soluble sugar levels might play roles in oat response to alkali stress,

Changes of Oxidative Stress and Soluble Sugar in Anthers Involve in Rice Pollen Abortion Under Drought Stress

Two rice maintaining lines with different drought tolerances, viz., Jin 23B （tolerant） and Zhenshan 97B （sensitive）, were used to study the oxidative stress and soluble sugar in rice anthers and pollen viability under drought stress during flowering stage. Higher antioxidant enzyme activities and lower malonaldehyde （MDA） content in rice anthers were observed in Jin 23B than in Zhenshan 97B under drought stress. Further, a great increase in the content of soluble sugar in rice anthers of Jin 23B was observed across the whole drought exposure, while Zhenshan 97B showed significant decrease in soluble sugar during 9-12 d after drought stress （DADS）. Accordingly, a marked decline of pollen fertility and activity, pollen numbers in an anther and pollen numbers on a stigma was observed in Zhenshan 97B, whereas little difference was found in Jin 23B. Thus, we suggest that pollen abortion caused by drought stress may be related with the reciprocity between oxidative stress and soluble sugar content in rice anthers.

Effect of Cadmium and Herbicides on the Growth, Chlorophyll and Soluble Sugar Content in Rice Seedlings

To investigate the combined effects of Cd^2＋ （ 0,10,100,500 μmol/L） , acetochlor （AC） （0,1.6,4.0,8.0μmol/L）, and bensulfuronmethyl （BSM） （ 0,0.16,0.40,0.80 μmol/L） on the biomass,leaf soluble sugar, total chlorophyll （chl） content, chl a/b ratio, and Cd content in roots and shoots in the rice seedlings, pot culture experiments were conducted with rice （Oryza sativa L. ） cultivar Jinyou 402. The results showed that the Cd and AC combined treatment （Cd＋ AC treatment） significantly inhibited the growth of the roots and shoots. The root dry weight/shoot dry weight （RDW/SDW） ratio, total chlorophyll content and chl a/b ratio decreased by 41%, 50%, 56% , respectively, in comparison with the control, as well as the leaf soluble sugar content, plant dry weight /plant fresh weight （PDW/PFW） ratio increased by 284% and 44% , respectively. Cd content in the roots and shoots had a decreasing tendency with the increasing concentration of AC under the Cd＋AC treatment. The Cd and BSM combined treatment （Cd＋BSM treatment） had a similar result to the Cd＋AC treatment. The results suggest that the toxicity of Cd to rice seedlings has been enhanced due to its combination with herbicides.

Identification of candidate genes related to soluble sugar contents in soybean seeds using multiple genetic analyses

Soluble sugar content in seeds is an important quality trait of soybean. In this study, 57 quantitative trait loci(QTLs) related to soluble sugar contents in soybean seeds were collected from databases and published papers. After meta-overview-collinearity integrated analysis to refine QTL intervals, eight consensus QTLs were identified. To further verify the consensus QTLs, a population of chromosome segment substitution lines(CSSLs) was analyzed. Two lines containing fragments covering the regions of consensus QTLs and the recurrent parent were selected: one line showed high soluble sugar contents associated with a consensus QTL fragment, and the other line showed low soluble sugar contents. Transcriptome sequencing was conducted for these two lines at the early, middle, and late stages of seed development, which identified 158, 109 and 329 differentially expressed genes, respectively. Based on the analyses of re-sequencing data of the CSSLs and the consensus QTL region, three candidate genes(Glyma.19 G146800, Glyma.19 G122500, and Glyma.19 G128500) were identified in the genetic fragments introduced from wild soybean. Sequence comparisons between the two CSSL parents SN14 and ZYD00006 revealed a single nucleotide polymorphism(SNP) mutation in the coding sequence of Glyma.19 G122500, causing a nonsynonymous mutation in the amino acid sequence that affected the predicted protein structure. A Kompetitive allele-specific PCR(KASP) marker was developed based on this SNP and used to evaluate the CSSLs. These results lay the foundation for further research to identify genes related to soluble sugar contents in soybean seeds and for future soybean breeding.

Change of 3 Kinds of Enzyme Activity and Soluble Sugar in 3 Chinese Cabbage Varieties with Different Resistance Level During the Process of Plasmodiophora brassicae Infecting Their Roots

There are different levels of resistance in Chinese cabbage varieties against clubroot.The content of catalase(CAT),peroxidase(POD),phenylalanin ammonia-lyase(PAL),Super Oxide Dismutase(SOD)and soluble sugar activities of 3cultivars with different clubroot-resistant levelswere detected after inoculation in this study.The results suggest that the changing rates of CAT,PAL and SOD contents of resistance cultivars after inoculation were higher than those of susceptible cultivars(Baigengbaiye>Zaobaicaitai>Huangjinxiaobaicai);the POD activities of resistance cultivars reached a peak on an earlier day than those of susceptible cultivars;the increasing rate of soluble sugar of susceptible cultivars was higher than that of the resistance cultivars,and ended with two peaks.Therefore,the four enzymes(CAT,POD,PAL and SOD)and soluble sugar may be used as physiological and biochemical reference indexes for the resistance identification to clubroot after inoculation with P.brassicae.

The difference of soluble sugar contents and starch synthetic key enzyme activities between high and low starch cultivar of cassava

The difference of soluble sugar contents and starch synthetic key enzyme activities between high starch cultivar and low starch cultivar were studied in this experiment, of which FuXuan01, GR891, SC124, and SC201 were used as materials. The results showed that the contents of reducing sugar in root tube of low starch cultivars were higher than those of high starch cultivars in all growth periods, the contents of sucrose in root tube of high starch cultivar were higher than those of low starch cultivars in the early growth period, but, lower in the late growth period, and there were almost no difference in the contents of soluble sugar in root tube between high starch cultivar and low starch cultivar in the early growth period, but notable difference in the late growth period and the soluble sugar contents of low starch cultivars were higher than those of high starch cultivars. It also-showed that the activities of ADPGPpase, SSS, and SBE （starch branching enzyme） of high starch cultivars were higher than those of low starch cultivars. It was evident that there were close correlations between the content of sucrose, reducing sugar and soluble sugar in root tube, and the activities of ADPGPpase, SSS, SBE, and the starch accumulation in the root tubers of cassava. It was quite evident in this experiment that the soluble sugar and starch synthetic key enzyme were main factors controlling starch accumulation in root tubers. These results provided important indication for physiological controlling in high starch cultivation and gene engineering breeding of high starch cultivar of cassava.

Profiling seed soluble sugar compositions in 1164 Chinese soybean accessions from major growing ecoregions

Soluble sugar is a key quality trait of soybean seeds.We developed rapid and economic extraction and quantification methods for seed soluble sugars using an ultra-high performance liquid chromatography system with a refractive index detector.We evaluated the soluble sugar compositions of 1164 soybean accessions collected from diverse ecoregions and grown in multiple locations and years.Total soluble sugar(TSS)content was influenced by accession type,year of cultivation,and ecoregion.The mean contents of fructose,glucose,sucrose,raffinose,stachyose and TSS were 3.31,5.21,55.60,6.60,35.47,and 106.19 mg g^(-1),respectively.The highest mean TSS content(108.71 mg g^(-1)) was observed in accessions from Northern Region of China.Cultivars contained higher contents of sucrose,raffinose,and TSS,whereas landraces had a higher content of stachyose.Fourteen accessions with mean TSS contents>130 mg g^(-1) were identified as elite soybean resources.TSS was correlated with sucrose,raffinose,stachyose,protein,oil and total tocopherol.The main soluble sugar components were correlated with latitude and longitude,indicating that the geographical origin of the accessions affected their seed soluble sugar compositions.The developed methods and elite identified accessions can be used in the food and feed industry and in soybean breeding programs aimed at improving soybean seed nutrition.

Effects of NaOH Treatment on Fiber, Crude Protein and Soluble Sugar Content of Corn Straw

[Objective] The paper was to study the effects of different levels of NaOH on fiber, crude protein and soluble sugar content of cornstraw. [Method] Four treatments were designed in the test, namely control group, group 1, group2 and group 3, which were added with 0, 2%, 3%and 4% NaOH according to the dry weight of corn straw, and the moisture of corn straw after treatment was 45%. After being treated at room tem-perature for 48 h, sensory evaluation and nutrient composition analysis were performed for each group, and the contents of neutral detergent fiber（NDF）, acid detergent fiber （ADF）, crude protein （CP） and soluble sugar （SS） were measured. [Result] With the increasing addition level of NaOH,the content of NDF and ADF decreased gradually, and significant differences were observed between group 3 （adding 4% NaOH） and control group（P〈0.05）. There was no significant difference in CP content among treatment groups（P〉0.05）; the SS content gradually increased, and there weresignificant differences between treatment groups and control group （P〈0.05）. [Conclusion] 4% NaOH reduced the fiber content and increased thesoluble sugar content of corn straw.

Study on Physiological Characteristics of Three Different Cold-resistant Tomato Strains

[Objective]The aim was to study cold-resistant indexes and physiological characters of three different cold-resisitant tomato strains selected under natural conditions. [Method]With 3-4 weeks tomato seedlings as tested materials,through simulating external environment conditions by the cold treatment in lab,the survival ratio and growth status of seedlings were identified; by studying the morphological change of in vitro leaves after cold treatment and determining the relative conductivity,the cold-resistant degree of different tomato strains was identified and the correlation between conductivity and survival rate was analyzed; the variation of soluble sugar and praline content in plant of tomato was determined under the condition of cold stress. [Result]The results showed that leaf morphological changes,size of conductivity and growth status of different tomato strains after cold treatment have significant differences; the variation of soluble sugar contents in plant has significant differences,while the variation of proline content has no significant differences. [Conclusion]There is a certain correlation between conductivity and survival rate under cold treatment conditions,and plant cold tolerance is related to accumulation of soluble sugar content.

Influences of 5-ALA Application on Winter Oilseed Rape (Brassica napus L.) Growth before Winter

[Objective] The aim of this paper is to investigate the influences of exogenous 5-aminolevulinic acid （5-aLA） application on the growth and mechanism of winter oilseed rape （Brassica napus L.） before winter. [Method] For this purpose a split-plot experimental design with four treatments of seed soaking with 0,25,50 and 75 mg/L ALA solution （CK,A1,A2 and A3） was carried out from October 2009 to January 2010 in order to observe the influences upon the growth and relevant physiological indexes of Zhongshuang 9. [Result] Seed soaking with ALA solution significantly promotes the growth of double-9 before winter,which is probably due to the fact that ALA application enhances its photosynthesis,absorption of nutrients,and its anti-oxidation abilities. The research also finds that ALA treatment notably increases its photosynthetic pigment contents,root activities,leaf nitrate reductase activities,soluble sugar contents,antioxidase activities,and significantly decreases malondialdehyde（MDA） content. [Conclusion] These results indicate that seed soaking with 50 mg/L ALA solution might have a potential application in increasing the productivity of winter oilseed rape in agricultural management.

Effects of Bagging with Paper Bags and Non-woven Bags on Flavor Compounds of Feicheng Peach

With Hongli Feicheng Peach as the test material, the effects of bagging with 4 kinds of paper bags and 3 kinds of non-woven bags on the soluble sugar, organic acids and aroma compounds in peach fruits were investigated using headspace solid-phase microextraction （HS-SPME） combined with gas chromatogra- phy-mass spectrometry （GC-MS） and high-performance liquid chromatography （HPLC）. The results showed that there were significant differences in sugar and acids contents of peach fruits between different bagging treatments. The sugar con- tent of peach fruits was increased when bagged with single-layer inner-black outer- yellow paper bags and double-layer inner-white outer-yellow paper bags. The organ- ic acid content in peach fruits bagged with all the types of bags except the single- layer inner-black outer-yellow paper bag was increased greatly. The count and rela- tive content of aroma compounds were all decreased after bagging. Combined with the detection results of soluble sugar, organic acids and aroma compounds, the sin- gle-layer inner-black outer-yellow paper bag was suitable for bagging of Hongli Fe- icheng peach.

Physical-chemical and Agronomical Analysis of a Leaf Color Mutant of Rice

[Objective] This study was to investigate the physical-chemical properties and the agronomical traits of a leaf color mutant K7231,so as to provide scientific basis for its use as breeding material.[Method]Before heading,the contents of chlorophyll and soluble sugar of leaf color mutant K7231 were measured,and its agronomic traits after mature were investigated.[Result]The contents of chlorophyll and soluble sugar of leaf color mutant K7231 before heading assumed a regular change law.Except the lower yield,K7231 performed similar agronomic traits compared with the CK.[Conclusion]This study provided scientific basis for the utilization of this mutant.

Effect of Different Fertilization Patterns on Buckwheat Seed Quality

[Objective] This study aimed to improve the taste and quality of buckwheat（Fagopyrum esculentum） seeds. [Method] Buckwheat seeds were fertilized by using a quadratic orthogonal rotation combination design to establish quaternary quadratic regression equations between N, P, K, organic fertilizers and soluble sugar, protein contents in buckwheat seeds. [Result] According to the regression equations, appropriately increasing the application amount of P fertilizer and maintaining the ratio of N fertilizer to organic fertilizer at 1：140 could improve soluble sugar and protein contents in buckwheat seeds. By simulation optimization using a computer,in order to ensure high soluble sugar and protein contents in buckwheat seeds, the optimal fertilization patterns of each fertilizer were： N, 535.35-581.10 kg/hm^2; P2O5,69.511 5-71.359 5 kg/hm^2; K2 O, 249.525-270.975 kg/hm^2; organic fertilizer, 76 590-80 514 kg/hm^2. The optimal fertilization ratio of N, P, K and organic fertilizers was8：1：4：1 114. [Conclusion] This study provided scientific basis for producing buckwheat products with good taste, high nutrition value and excellent quality.

Comparison of Biochemical Substances in the Fiber Development of Different Cotton Varieties

Zhongmian 42 and Xinluzao 36 were used as raw materials to determine the contents of soluble sugar and protein, as well as dynamic changes of enzyme activities after flowering during cotton fiber growth. The results showed that contents of soluble protein in the two species sharply declined 7 to 21 days after flowering, as the soluble sugar in Zhongmian 42 leveling off after 21 days of flowering while the soluble sugar in Xinluzao 36 dropped notably after 21 days of flowering before remaining stable after seven days later. The soluble sugar decreased 7 to 14 days after flowering before sharply rising to the maximum seven days later, and then began to decline quickly. The soluble sugar was the minimum after 35 days of flowering and then remaining stable. Peroxidase activity generally increased. Indole-3- acetic acid oxidase activities were low at 7 days after flowering. IAAO activity reached to the peaks on the 14th and 28th day after flowering. IAAO activity of two varieties decreased with the same trend 35 days after flowering.