Soybean somatic cell could induce the development of embryoid which was similar to embryo morphologically and structurally. Somatic embryogenesis system of soybean was used to conduct genetic transformation of soybean...Soybean somatic cell could induce the development of embryoid which was similar to embryo morphologically and structurally. Somatic embryogenesis system of soybean was used to conduct genetic transformation of soybean because of its several advantages such as higher transformational efficiency, beetter synchronism and fewer plant chimeras among transgenic plants. After infected with agrobacterium tumefaciens,the initiation, differentiation and development of young cotyledon embryogenic cell of soybean which was cultured on selective culture medium with kanamycin were investigated through histological study. The result showed that somatic embryo was differentiated in non-bud differentiation way. The embryogenic cells were differentiated from epidermis of explant or cells in 1 layer or 2 layers, with the division of embryogenic cells and degradation and disorganization of surrounding cells, the embryogenic cells would form embryoid with analogous suspensor structure. Later, globular embryoid would extrude from epidermis then developed into heart-shape embryo. The experiment was expected to provide theoretical reference for the construction of high transformational system of using plant somatic embryogenesis induced by young cotyledon of soybean.展开更多
The differentiation process including somatic embryogenesis in different Ginkgo explants in vitro culture were studied by cytological observation.The results are as follows:1) two complete cotyledons and a embryo bud ...The differentiation process including somatic embryogenesis in different Ginkgo explants in vitro culture were studied by cytological observation.The results are as follows:1) two complete cotyledons and a embryo bud were observed in mature embryos and several secretory acavitives appeared in maturation region of embryo buds,hypocotyls,cotyledons and radicles after culturing 20 days;two incomplete cotyledons and a embryo bud primordia were found in large cotyledon embryos.The proembryo of two cells,four cells, multi-cellular,and globular embroy were developed from the callus of the small cotyledon embryos.2) The differentiation of cotyledon explants started from epidermal cells,and gradually formed meristematic cell mass in the cortical cells,and eventually adventitious buds were observed.3) The adventitious roots of Ginkgo originated in the cells at the cross of vascular cambium and vascular rays. 4) The type of rooting belongs to induction type by root primordium.The formed adventitious roots were observed after 20 days.展开更多
How to regenerate whole plants from single cell clones and establish cell lines with vigorous growth and differentiation potential is now becoming one of the absorbing topics in plant tissue culture. Since somatic emb...How to regenerate whole plants from single cell clones and establish cell lines with vigorous growth and differentiation potential is now becoming one of the absorbing topics in plant tissue culture. Since somatic embryos could develop from single cells, the regeneration of them has been proved to be the most efficient approach with rapid propagation ability, which would be very useful in cytology, genetics and plant breeding studies. Foxtail millet is one of the main Cereals, so far, no report about the formation of its somatic embryos has been found in refer ence.展开更多
Maturing seeds were collected from fifty-year-old trees of Fraxinus mandshurica. The cotyledon and hypocotyl zygotic embryos were excised and cultured on 1/2MS media to induce somatic embryos. In cytological study of ...Maturing seeds were collected from fifty-year-old trees of Fraxinus mandshurica. The cotyledon and hypocotyl zygotic embryos were excised and cultured on 1/2MS media to induce somatic embryos. In cytological study of embryo development, by the way of paraffin, somatic and zygotic embryogenesis was observed and compared under light microscope. The results showed that somatic embryos from zygotic cotyledons originated directly from single epidermis cell. The somatic embryos that indirectly occurred on the embryogenic callus came from its singular surface cell or internal multi_cells. There was an obvious cytological difference between embryogenic and non-embryogenic callus. Somatic and zygotic embryogenesis underwent the similar course, passing through proembryo, heart, torpedo and cotyledonary stages. The important differences in morphogenetic process exited as following: volume of somatic embryos was smaller than its partner at the same development stage; somatic embryos had no obvious suspensors; somatic embryos showed morphologic variations.展开更多
基金the National Natural Science Foundation of China (C02020504)the Scientific and Techrological Developing Scheme of Jilin Province (20050217-2+1 种基金20060204)the national 863 project (2006AA100104-17)~~
文摘Soybean somatic cell could induce the development of embryoid which was similar to embryo morphologically and structurally. Somatic embryogenesis system of soybean was used to conduct genetic transformation of soybean because of its several advantages such as higher transformational efficiency, beetter synchronism and fewer plant chimeras among transgenic plants. After infected with agrobacterium tumefaciens,the initiation, differentiation and development of young cotyledon embryogenic cell of soybean which was cultured on selective culture medium with kanamycin were investigated through histological study. The result showed that somatic embryo was differentiated in non-bud differentiation way. The embryogenic cells were differentiated from epidermis of explant or cells in 1 layer or 2 layers, with the division of embryogenic cells and degradation and disorganization of surrounding cells, the embryogenic cells would form embryoid with analogous suspensor structure. Later, globular embryoid would extrude from epidermis then developed into heart-shape embryo. The experiment was expected to provide theoretical reference for the construction of high transformational system of using plant somatic embryogenesis induced by young cotyledon of soybean.
文摘The differentiation process including somatic embryogenesis in different Ginkgo explants in vitro culture were studied by cytological observation.The results are as follows:1) two complete cotyledons and a embryo bud were observed in mature embryos and several secretory acavitives appeared in maturation region of embryo buds,hypocotyls,cotyledons and radicles after culturing 20 days;two incomplete cotyledons and a embryo bud primordia were found in large cotyledon embryos.The proembryo of two cells,four cells, multi-cellular,and globular embroy were developed from the callus of the small cotyledon embryos.2) The differentiation of cotyledon explants started from epidermal cells,and gradually formed meristematic cell mass in the cortical cells,and eventually adventitious buds were observed.3) The adventitious roots of Ginkgo originated in the cells at the cross of vascular cambium and vascular rays. 4) The type of rooting belongs to induction type by root primordium.The formed adventitious roots were observed after 20 days.
文摘How to regenerate whole plants from single cell clones and establish cell lines with vigorous growth and differentiation potential is now becoming one of the absorbing topics in plant tissue culture. Since somatic embryos could develop from single cells, the regeneration of them has been proved to be the most efficient approach with rapid propagation ability, which would be very useful in cytology, genetics and plant breeding studies. Foxtail millet is one of the main Cereals, so far, no report about the formation of its somatic embryos has been found in refer ence.
文摘Maturing seeds were collected from fifty-year-old trees of Fraxinus mandshurica. The cotyledon and hypocotyl zygotic embryos were excised and cultured on 1/2MS media to induce somatic embryos. In cytological study of embryo development, by the way of paraffin, somatic and zygotic embryogenesis was observed and compared under light microscope. The results showed that somatic embryos from zygotic cotyledons originated directly from single epidermis cell. The somatic embryos that indirectly occurred on the embryogenic callus came from its singular surface cell or internal multi_cells. There was an obvious cytological difference between embryogenic and non-embryogenic callus. Somatic and zygotic embryogenesis underwent the similar course, passing through proembryo, heart, torpedo and cotyledonary stages. The important differences in morphogenetic process exited as following: volume of somatic embryos was smaller than its partner at the same development stage; somatic embryos had no obvious suspensors; somatic embryos showed morphologic variations.
