In this study,embryogenic cell aggregates obtained from the established embryogenic cell suspension culture system of Anthurium andraeanum‘Alabama’were used as experimental materials to investigate the effects of ka...In this study,embryogenic cell aggregates obtained from the established embryogenic cell suspension culture system of Anthurium andraeanum‘Alabama’were used as experimental materials to investigate the effects of kanamycin and hygromycin on survival rate of cell aggregates,somatic embryogenesis and plantlet regeneration of A.andraeanum.According to the results,at the embryonic cell propagation stage,lethal doses of kanamycin and hygromycin to embryogenic cell aggregates of A.andraeanum were 200 and 60 mg/L,respectively;at the differentiation stage,either 150 mg/L kanamycin or 40 mg/L hygromycin could inhibit somatic embryogenesis;either 100 mg/L kanamycin or 20 mg/L hygromycin could inhibit plantlet regeneration.These results provided important reference for further studies of transgenic A.andraeanum.展开更多
基金Supported by Special Fund of President of the Guangdong Academy of Agricultural Sciences(201217)
文摘In this study,embryogenic cell aggregates obtained from the established embryogenic cell suspension culture system of Anthurium andraeanum‘Alabama’were used as experimental materials to investigate the effects of kanamycin and hygromycin on survival rate of cell aggregates,somatic embryogenesis and plantlet regeneration of A.andraeanum.According to the results,at the embryonic cell propagation stage,lethal doses of kanamycin and hygromycin to embryogenic cell aggregates of A.andraeanum were 200 and 60 mg/L,respectively;at the differentiation stage,either 150 mg/L kanamycin or 40 mg/L hygromycin could inhibit somatic embryogenesis;either 100 mg/L kanamycin or 20 mg/L hygromycin could inhibit plantlet regeneration.These results provided important reference for further studies of transgenic A.andraeanum.
