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苦参碱调节RhoA-ROCK信号通路对冠心病模型大鼠Th17/Treg细胞平衡的影响
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作者 哈斯高娃 乌吉斯古楞 《中药新药与临床药理》 CAS CSCD 北大核心 2024年第3期349-357,共9页
目的探讨苦参碱(Matrine)对冠心病(coronary heart disease,CHD)大鼠辅助T细胞17(helper T cell 17,Th17)/调节性T细胞(regulatory T cells,Treg)细胞平衡及Ras同源基因家族成员A(RhoA)-Rho相关的卷曲螺旋激酶(ROCK)信号通路的影响。方... 目的探讨苦参碱(Matrine)对冠心病(coronary heart disease,CHD)大鼠辅助T细胞17(helper T cell 17,Th17)/调节性T细胞(regulatory T cells,Treg)细胞平衡及Ras同源基因家族成员A(RhoA)-Rho相关的卷曲螺旋激酶(ROCK)信号通路的影响。方法建立冠心病模型,将实验大鼠分为对照组、模型组、苦参碱低剂量(50 mg·kg^(-1))组、苦参碱高剂量(200 mg·kg^(-1))组及苦参碱高剂量(200 mg·kg^(-1))+LPA组(10 mg·kg^(-1))。超声心动图进行大鼠心功能检测;酶联免疫吸附试验(enzyme linked immunosorbent assay,ELISA)法进行白细胞介素17(IL-17)、转化生长因子β(TGF-β)水平检测;流式细胞术检测Th17、Treg数量及Th17/Treg比值;免疫组化进行内皮型一氧化氮合酶(eNOS)、内皮素1(ET-1)蛋白表达水平检测;Masson染色进行大鼠心肌组织的病理形态变化观察;TTC染色检测各组大鼠心肌梗死情况;TUNEL染色进行心肌组织中细胞凋亡情况检测;试剂盒检测RhoA活性;Western Blot法进行半胱氨酸天冬氨酸蛋白酶3(Caspase-3)、B细胞淋巴瘤因子2(Bcl-2)、Bcl-2相关X蛋白(Bax)、RhoA、ROCK1、ROCK2蛋白表达水平检测。结果与对照组比较,模型组心肌组织有大量蓝色胶原纤维沉积,左室舒张末期容积(left ventricular end-diastolic volume,LVEDV)、左室收缩末期容积(left ventricular end-systolic volume,LVESV)、IL-17、Th17、Th17/Treg、ET-1、心肌梗死面积、细胞凋亡率、TUNEL阳性率、Bax、Caspase-3、RhoA活性、RhoA、ROCK1、ROCK2表达水平明显升高,左室射血分数(left ventricular ejection fraction,LVEF)、左室缩短分数(left ventricular shortening fraction,LVFS)、TGF-β、Treg、eNOS、Bcl-2表达水平明显降低(P<0.05)。与模型组比较,Matrine-L组、苦参碱高剂量组心肌组织蓝色胶原纤维逐渐减少,LVEDV、LVESV、IL-17、Th17、Th17/Treg、ET-1、心肌梗死面积、细胞凋亡率、TUNEL阳性率、Bax、Caspase-3、RhoA活性、RhoA、ROCK1、ROCK2表达水平依次明显降低,LVEF、LVFS、TGF-β、Treg、eNOS、Bcl-2表达水平依次明显升高(P<0.05)。与苦参碱高剂量组比较,苦参碱高剂量+LPA组心肌组织蓝色胶原纤维增多,LVEDV、LVESV、IL-17、Th17、Th17/Treg、ET-1、心肌梗死面积、细胞凋亡率、TUNEL阳性率、Bax、Caspase-3、RhoA活性、RhoA、ROCK1、ROCK2表达水平明显升高,LVEF、LVFS、TGF-β、Treg、eNOS、Bcl-2表达水平明显降低(P<0.05)。结论苦参碱通过抑制RhoAROCK信号通路调节Th17/Treg细胞平衡,改善冠心病大鼠心肌损伤。 展开更多
关键词 苦参碱 冠心病 Ras同源基因家族成员A-Rho相关的卷曲螺旋激酶信号通路(RhoA-rock) 辅助T细胞17/调节性T细胞(Th17/Treg) 心肌损伤 大鼠
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THE ROCK ASSOCIATION IN JINSHAJIANG MELANGE BELT
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作者 Lin Shiliang (Chengdu Institute of Geology and Mineral Resources,Chengdu 610082,China) 《地学前缘》 EI CAS CSCD 2000年第S1期126-126,共1页
Jinshajiang melange belt locates between Jianda\|Weixi island arc and Zhongzha massif. The melange belt and island arc makes up Jinshajiang plate junction. Although subsequent tectonic movements had complexed the stru... Jinshajiang melange belt locates between Jianda\|Weixi island arc and Zhongzha massif. The melange belt and island arc makes up Jinshajiang plate junction. Although subsequent tectonic movements had complexed the structural form of Jinshajiang melange belt, there are still a lots of structural block remained which carried amount of information about the tectonic evolution of the belt. Recent researches have identified several kinds of rock association in the structural blocks.(1) Ophiolite:The ophiolite consists of serpentinization ultramafite, ultramafic cumulus crystal rock (pyroxenite, dunite), gabbro, diabase cluster, ocean\|ridge type basalt, plagiogranite and radiolarian silicalite. The isotopic age shows that the ultramafite and basalt formed during Upper Carboniferous and Lower Permian. The silicalite is high in radiolaria of Lower Permian.(2) Rock association of oceanic island\|arc:The liptocoenosis of oceanic island\|arc scatter in melange belt, it mainly consists of sandy slate, pyroclastic rock, silicalite, basalt and andesite. A part of volcanic rock belongs to calc\|alkaline volcanic suite and the other is tholeiite. The petrochemistry, REE and microelement of volcanic rock have the feature of the rock in ocean\|island arc. The isotopic age of basalt shows that the ocean\|island arc formed in Lower Permian. 展开更多
关键词 rock association Jinshajiang MELANGE BELT OPHIOLITE structura l BLOCKS
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The Middle Devonian Bimodal Association of Volcanic Rocks in the Northern Area of East Junggar, Xinjiang
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作者 Han Baofu Department of Geology, Peking University, Beijing 《Acta Geologica Sinica(English Edition)》 SCIE CAS CSCD 1992年第2期141-153,共13页
The Middle Devonian volcanic rocks in the northern area of East Junggar, located between the Ertix andUlungur rivers of northern Xinjiang, may be divided into basic and acid ones. It is evident that a compositionalgap... The Middle Devonian volcanic rocks in the northern area of East Junggar, located between the Ertix andUlungur rivers of northern Xinjiang, may be divided into basic and acid ones. It is evident that a compositionalgap exists between the two groups so that the volcanic rocks are not in line with a calc-alkaline series becausethe intermediate rocks are absent in the area. The fact shows that the volcanic rocks are a typical bimodal asso-ciation. The formation of the bimodal association of volcanic rocks in the area was closely related to continen-tal rifting or continental extension in the Middle Devonian. In such a tectonic setting, magmas were first pro-duced by partial melting of the mantle. Where crustal thinning was greater, the magmas ascended and eruptedon the surface directly so that the basic volcanic rocks formed, but olivine and/or partial pyroxenefractionation occurred in the magmas during their ascent through the thinning crust. On the other hand, wherecrustal thinning was less, ascending mantle-derived magmas reached the lower crust and accumulated there, re-sulting in partial melting of the lower crust and thus giving rise to the contaminated magma which was consoli-dated as acid volcanic rocks on the surface. 展开更多
关键词 rock REE area The Middle Devonian Bimodal association of Volcanic rocks in the Northern Area of East Junggar XINJIANG
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藁本内酯通过抑制RhoA/ROCK信号通路改善脂多糖诱导小鼠肾足细胞损伤
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作者 顾海燕 王仲建 《广州中医药大学学报》 CAS 2024年第6期1573-1581,共9页
【目的】探讨藁本内酯对脂多糖(LPS)诱导小鼠肾足细胞损伤的改善作用及机制。【方法】(1)体内实验:将小鼠随机分为空白对照组,LPS诱导组,藁本内酯低、高剂量组及藁本内酯高剂量+pcDNA-NC(RhoA阴性对照)组、藁本内酯高剂量+pcDNA-RhoA(R... 【目的】探讨藁本内酯对脂多糖(LPS)诱导小鼠肾足细胞损伤的改善作用及机制。【方法】(1)体内实验:将小鼠随机分为空白对照组,LPS诱导组,藁本内酯低、高剂量组及藁本内酯高剂量+pcDNA-NC(RhoA阴性对照)组、藁本内酯高剂量+pcDNA-RhoA(RhoA过表达)组。除空白对照组,其他各组小鼠采用LPS诱导法构建急性肾损伤(AKI)模型。干预结束后,检测小鼠血清中尿素氮(BUN)和肌酐(Cr)水平,高碘酸-希夫(PAS)染色法观察小鼠肾脏组织的病理变化,Western Blot法检测肾脏组织中RhoA、Rho相关卷曲螺旋形成蛋白激酶(ROCK)蛋白的表达。(2)体外实验:将培养的小鼠足细胞MPC5分为空白对照组,LPS诱导组,藁本内酯低、高剂量组,藁本内酯高剂量+pcDNA-NC组,藁本内酯高剂量+pcDNA-RhoA组,细胞计数试剂盒8(CCK-8)法检测足细胞增殖活性,流式细胞术检测细胞凋亡,鬼笔环肽染色法分析细胞骨架,Western Blot法检测细胞RhoA、ROCK蛋白的表达。【结果】与空白对照组比较,LPS诱导组小鼠血清中BUN和Cr的水平、肾小管损伤评分、RhoA和ROCK蛋白表达水平升高(P<0.05);与LPS诱导组比较,藁本内酯低、高剂量组小鼠血清中BUN和Cr水平、肾小管损伤评分、RhoA和ROCK表达水平降低(P<0.05)。与空白对照组比较,LPS诱导组MPC5细胞增殖活性、F-肌动蛋白荧光强度、RhoA和ROCK的表达降低(P<0.05),凋亡率升高(P<0.05);与LPS诱导组比较,藁本内酯低、高剂量组MPC5细胞增殖活性、F-肌动蛋白荧光强度、RhoA和ROCK的表达升高(P<0.05),凋亡率降低(P<0.05)。利用过表达RhoA进行回补实验发现,RhoA过表达逆转了藁本内酯对LPS诱导小鼠肾组织和足细胞损伤的缓解作用(P<0.01)。【结论】藁本内酯可能通过抑制RhoA/ROCK信号通路改善LPS诱导小鼠肾足细胞损伤。 展开更多
关键词 藁本内酯 急性肾损伤 RhoA/Rho相关卷曲螺旋形成蛋白激酶(rock)通路 脂多糖 足细胞 小鼠
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The Sachakou Deposit in West Kunlun of Xinjiang: A Pb-Zn Polymetallic Deposit Associated with Magmatic Metasomatism of Carbonate Rock 被引量:1
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作者 ZHOU Menglin LIU Yingchao 《Acta Geologica Sinica(English Edition)》 CAS CSCD 2018年第2期883-884,共2页
Objective The Sachakou Pb-Zn polymetallic deposit is located in Hetian County, Xinjiang (geographical coordinates of E78° 57' 54.30"-78°59' 53.63", N34° 39' 27.50"-34° 40' 57.21"). It be... Objective The Sachakou Pb-Zn polymetallic deposit is located in Hetian County, Xinjiang (geographical coordinates of E78° 57' 54.30"-78°59' 53.63", N34° 39' 27.50"-34° 40' 57.21"). It belongs to the West Kunlun orogenic belt on the northwest edge of the Qinghai-Tibet Plateau and is connected to the Sanjiang orogenic belt to the south (Spurlin et al., 2005). In recent years, a series of Pb-Zn mineralized spots and deposits have been discovered in this area one after another, which is called the Huoshaoyun ore concentration area. Among them, the Sachakou Pb-Zn deposit has reserves up to140 Mt, which has reached a large scale. However, the study on the genesis of deposits in this area has only just begun. This work studied the genesis ofthis Pb-Zn deposit in order to provide new ideas for the genesis of regional deposits and regional prospecting. 展开更多
关键词 PB A Pb-Zn Polymetallic Deposit associated with Magmatic Metasomatism of Carbonate rock The Sachakou Deposit in West Kunlun of Xinjiang ZN
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Energy analysis of rock plug thickness in karst tunnels based on non-associated flow rule and nonlinear failure criterion 被引量:6
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作者 杨子汉 张睿 +1 位作者 许敬叔 杨小礼 《Journal of Central South University》 SCIE EI CAS CSCD 2017年第12期2940-2950,共11页
The geological hazards, such as water inrush and mud outburst, are easily induced by the high water pressure caverns ahead of a karst tunnel face. Therefore, it is a pivotal issue to determine the reserved thickness o... The geological hazards, such as water inrush and mud outburst, are easily induced by the high water pressure caverns ahead of a karst tunnel face. Therefore, it is a pivotal issue to determine the reserved thickness of rock plug during the construction of tunnels. The limit analysis principle is employed to analyze the safe thickness from the point of energy dissipation, and the nonlinear and non-associated characteristics of geotechnical materials are both considered. On the basis of a plane failure pattern of rock plug, the expressions of detaching curve and rock plug thickness are derived. The effect of each parameter on the safe thickness of rock plug is discussed in detail, which interprets the corresponding failure scope of rock plug. The obtained results indicate that the thickness of rock plug is highly influenced by the nonlinear dilatancy coefficient and the nonlinear coefficient. The proposed method is validated by a comparison of the calculated results with those of the engineering project of the "526 karst cavern" of Yunwushan tunnel. This proposed method can provide reference basis for the design and excavation of karst tunnels in the future. 展开更多
关键词 KARST tunnel water inrush safe thickness of rock plug UPPER BOUND THEOREM nonlinear failure criterion non-assoc flow RULE
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ARTICLE Petrographic and Mineralisation Potentials of Precambrian Pegmatities and Associated Rock Units of Olode Area,Southwestern Nigeria
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作者 S.I.Okonkwo S.O.Idakwo 《Journal of Geological Research》 2020年第4期62-74,共13页
The geology of the Olode area, south-western Nigeria was investigatedpetrographically and geochemically in order to elucidate the mineralisationpotential of the rock units in the area. The area under study is generall... The geology of the Olode area, south-western Nigeria was investigatedpetrographically and geochemically in order to elucidate the mineralisationpotential of the rock units in the area. The area under study is generallychange to underlained by granite gneiss, mica schist and pegmatites.Petrographical studies indicated prevalence of anhedral quartz (30 - 50%),plagioclase (14 - 20%), orthoclase (12 - 15%), muscovite (11 - 15%),tourmaline (6 - 10) and other minerals (8 - 11%) for the pegmatite. Thehigh value of SiO2 and Al2O3 is consistent with the petrographical study.High values and wide range in Ba (34 - 737 ppm) and Zr (3.8 - 132.6 ppm)strongly support a mixture of igneous and sedimentary. The bivariant plotsof Rb vs K/Rb, Zn vs K/Rb and Th vs K/Rb indicated a partial series offractionation, suggesting that the pegmatites are of rare element classeswhile granite gneiss and mica schist belong to the barren muscovite andrare element classes. This was supported by high ratios of K/Cs and K/Babut low Th/U values indicating distinctively low rare metal mineralization.The plot of Na2O/Al2O3 vs K2O/Al2O3 revealed an igneous precursor for allthe rock units. The negative Eu anomalies especially in the pegmatitic rockunit indicates fractionation and point toward a late metasomatic effect andtheir relatively weak negative Ce anomalies, also suggest their rare metalmineralization. The pegmatites, granite gneiss and mica schist of Olodearea considered as barren as all the samples plotted below the Gordiyenkosand Beus’ line of mineralization. 展开更多
关键词 Pegmatite and associated rocks Geochemistry Mineralisation Gordiyenkos and Beus’line Olode
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腺病毒介导的RNAi对VaD大鼠脑内NgR/RhoA/ROCK2信号通路的影响
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作者 邓秋媚 向军军 +4 位作者 吴林 莫雪妮 陈炜 黎军宏 胡跃强 《中国老年学杂志》 CAS 北大核心 2023年第18期4456-4460,共5页
目的探讨基于腺相关病毒为载体构建的Nogo受体(NgR)抑制剂通过NgR/Ras基因家族成员(Rho)A/Rho相关激酶(ROCK)2信号通路改善血管性痴呆(VaD)大鼠学习记忆能力及轴突再生分子机制。方法将40只SD大鼠随机分为假手术组、模型组、NgR干扰剂组... 目的探讨基于腺相关病毒为载体构建的Nogo受体(NgR)抑制剂通过NgR/Ras基因家族成员(Rho)A/Rho相关激酶(ROCK)2信号通路改善血管性痴呆(VaD)大鼠学习记忆能力及轴突再生分子机制。方法将40只SD大鼠随机分为假手术组、模型组、NgR干扰剂组(腺相关病毒)、阴性对照组(NgR空载体病毒),每组10只。采用双侧颈总动脉永久结扎术法制作VaD大鼠模型,模型成功后,将AAV9-NgR-shRNA通过脑立体定位术注射至大鼠海马组织,阴性对照组注入等量空载体腺相关病毒。4 w后,采用Morris水迷宫测定学习、记忆能力,采用实时荧光定量-聚合酶链反应(PCR)、Western印迹检测各组NgR/RhoA/ROCK2通路NgR、RhoA、ROCK2 mRNA及蛋白表达水平,电镜观察大鼠海马突触超微结构变化。结果术后1 w,模型组、阴性对照组潜伏期时间与跨越平台次数与NgR干扰组比较,差异无统计学意义(P>0.05),与假手术组比较有显著性差异(均P<0.05)。术后4 w,与模型组及阴性对照组比较,NgR干扰组潜伏期显著缩短,跨越平台次数显著增加,海马中NgR、RhoA、ROCK2 mRNA及其蛋白表达显著减少(均P<0.05),且海马组织内突触前后囊泡正常、细胞器更加完整;阴性对照组上述指标与模型组比较差异无统计学意义(均P>0.05)。结论NgR抑制剂可能通过抑制NgR/RhoA/ROCK2信号通路起到促进神经轴突再生,改善海马突触结构,减轻VaD大鼠认知功能障碍的作用。 展开更多
关键词 血管性痴呆 突触再生 Nogo受体(NgR)/Ras基因家庭成员(Rho)A/Rho相关激酶(rock)2通路
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ROCKⅡ与子痫前期发病的关系 被引量:3
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作者 董潇 尚涛 《中国全科医学》 CAS CSCD 2008年第1期69-72,共4页
为了解ROCKⅡ与子痫前期发病的关系,查阅国内外文献并对文献进行整理,发现ROCKⅡ在子痫前期发病过程中起一定作用,现就Rho/ROCK信号传导通路与妊娠的关系做一综述。
关键词 RHO (ras homologus oncogenes) rock (Rho associated coiled COIL forming protein kinase) 子痫前期
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ROCK蛋白在肝癌中的表达及临床意义 被引量:8
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作者 程朋 李焱 +5 位作者 谭勇 高辉 彭晶晶 张涛 魏东 苏晓妹 《西南国防医药》 CAS 2011年第2期138-140,共3页
目的了解肝癌组织中Rho相关卷曲螺旋形成蛋白激酶(ROCK)表达水平,探讨其在肝癌组织中表达的临床意义。方法在60例肝癌标本中,采用ROCK蛋白特异标记物抗体进行免疫组化染色,了解肝癌组织中ROCK的表达及分布情况,结合病例的年龄、性别、... 目的了解肝癌组织中Rho相关卷曲螺旋形成蛋白激酶(ROCK)表达水平,探讨其在肝癌组织中表达的临床意义。方法在60例肝癌标本中,采用ROCK蛋白特异标记物抗体进行免疫组化染色,了解肝癌组织中ROCK的表达及分布情况,结合病例的年龄、性别、分期、转移情况,了解ROCK蛋白表达情况与不同临床病例参数之间的关系。结果肝癌组织中ROCK蛋白表达明显高于正常肝组织,ROCK蛋白的表达在分期较晚及有转移的病例中较高,并具有统计学意义(P<0.05)。结论肝癌组织中,ROCK蛋白的高表达对肿瘤的浸润和转移起到一定作用。 展开更多
关键词 Rho相关卷曲螺旋形成蛋白激酶 肝癌 Rho/rock
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MRS2578通过P2Y6-ROCK-MILC信号通路抑制海马CA3区域小胶质细胞吞噬作用并加重小鼠癫痫发作 被引量:1
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作者 沈晓华 胡泊 +2 位作者 唐洁 徐龙 朱佳 《中国生物化学与分子生物学报》 CAS CSCD 北大核心 2020年第11期1312-1320,共9页
许多研究表明,癫痫过程中P2Y6受体表达增加。MRS2578作为P2Y6受体的不可逆抑制剂,其在癫痫中作用尚不明确。本研究旨在阐明MRS2578在癫痫中的作用及其潜在的分子机制。研究发现,小鼠脑室注射海人酸诱导癫痫模型,同时注射MRS2578能加重... 许多研究表明,癫痫过程中P2Y6受体表达增加。MRS2578作为P2Y6受体的不可逆抑制剂,其在癫痫中作用尚不明确。本研究旨在阐明MRS2578在癫痫中的作用及其潜在的分子机制。研究发现,小鼠脑室注射海人酸诱导癫痫模型,同时注射MRS2578能加重癫痫发作,此作用呈现浓度依赖效应;MRS2578增加海马CA3区域神经元丢失(FJB+神经元由71.67±8.88增加至98.45±9.97个/视野);同时抑制小胶质细胞的激活以及转变为阿米巴样细胞(小胶质细胞密度由66.18±5.86下降至55.22±10.85个/视野),小胶质细胞CD68表达也相应降低;共聚焦显微镜检测发现,MRS2578处理后,CA3区域小胶质细胞吞噬细胞所形成的"吞噬杯"结构减少;Western印迹检测证实,MRS2578抑制小胶质细胞P2Y6、Rho相关激酶(ROCK)、p-MLC-Ser19的分子表达。上述结果提示,MRS2578加重海人酸诱导的癫痫发作,伴随小胶质细胞激活的抑制和细胞吞噬功能下调,此过程依赖P2Y6-ROCK-MLC信号途径。 展开更多
关键词 MRS2578 P2Y6 癫痫 小胶质细胞 吞噬 Rho相关激酶 肌球蛋白轻链
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我国矿产资源综合利用现状评估与发展路径
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作者 鞠建华 韩见 冯聪 《中国矿业》 北大核心 2024年第6期14-25,共12页
我国各类矿产资源储量规模和结构不均衡,大型单一富矿不足,共伴生、低品位矿多,这种资源禀赋特点决定了必须走综合利用的发展路线和模式。经过多年努力,我国已形成全球最完整的矿产资源勘查、采选、冶炼、加工和应用产业体系,综合开发... 我国各类矿产资源储量规模和结构不均衡,大型单一富矿不足,共伴生、低品位矿多,这种资源禀赋特点决定了必须走综合利用的发展路线和模式。经过多年努力,我国已形成全球最完整的矿产资源勘查、采选、冶炼、加工和应用产业体系,综合开发利用水平显著提升,油气采收率高位平稳,煤炭平均开采回采率达到70%,铁矿开采回采率和选矿回收率保持在80%左右,有色金属矿种采选指标稳中有升,尾矿废石综合利用步伐加快,缓解了生态环境压力。我国矿产资源供应能力基本保持稳定,为经济社会发展和平稳运行发挥了重要保障作用。近年来,矿产资源供需矛盾依然十分突出,部分矿产增储上产乏力或有下降趋势,产业链上游压力增大,大力提升矿产资源综合利用水平是保障我国资源安全供给的必然选择,也是推进矿业领域生态文明建设和高质量发展的必由之路,更是培育发展矿业新质生产力的重要载体和抓手。本文较为客观地评价了我国矿产资源综合利用基本现状,分析了存在的问题与挑战,认为我国矿产资源综合利用潜力大、前景好,并提出了全面构建矿产资源综合利用五大支撑体系的发展路径。 展开更多
关键词 矿产资源 综合利用 共伴生矿 废石 尾矿 支撑体系
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超固结黏土中不排水柱孔扩张弹塑性解及应用
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作者 吕桂阳 和西良 +5 位作者 梁汝鸣 付艳斌 刘洋 葛序尧 祝人杰 张波 《工程力学》 EI CSCD 北大核心 2024年第1期160-170,共11页
为求得超固结黏土中考虑K0影响的柱孔不排水扩张精确解,该文基于黏土和砂土模型(CASM)屈服准则,采用经典的应力剪胀关系,充分考虑土体静止土压力参数K0和重度超固结比,运用非相关联流动法则进行应力空间转换,进而建立超固结黏土中考虑K... 为求得超固结黏土中考虑K0影响的柱孔不排水扩张精确解,该文基于黏土和砂土模型(CASM)屈服准则,采用经典的应力剪胀关系,充分考虑土体静止土压力参数K0和重度超固结比,运用非相关联流动法则进行应力空间转换,进而建立超固结黏土中考虑K0影响情况下柱孔不排水扩张问题的一阶偏微分方程组求解模型。根据弹塑性边界已知的应力和位移条件,通过数值求解方法确定柱孔扩张模型的精确解。对比修正剑桥模型(MCC)解,基于SMP准侧改进的修正剑桥模型(SMP-MCC)解及黏土和砂土统一硬化模型(CSUH)解进行参数分析表明:当土体OCR≤2时,该文方法较好地模拟土体单元屈服应力和破坏应力;当土体OCR>2时,该文方法克服了超固结黏土高估屈服应力和破坏应力;当K0由1.0变化到2.5时,归一化极限扩孔压力由5.4变化到3.2,归一化极限超静孔隙水压力由2.4变化到1.0。并通过现场实测数据验证了该文方法的实用性与正确性。 展开更多
关键词 岩土力学 柱孔扩张理论 本构模型 超固结黏土 非相关联流动法则
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异丙酚通过Rho/Rock信号通路调控大鼠呼吸机相关性肺损伤的机制研究
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作者 朱帅 官彬 +1 位作者 周艳楠 童华 《解剖学研究》 CAS 2019年第4期286-289,共4页
目的探讨异丙酚通过Rho/Rock信号通路调控大鼠呼吸机相关性肺损伤的机制。方法 40只成年雄性SD大鼠随机分为4组,每组10只,分别为双肺通气组(TLV组),单肺通气组(OLV足),单肺通气+异丙酚组(D组),单肺通气+异丙酚+法舒地尔组(DL组)。对各... 目的探讨异丙酚通过Rho/Rock信号通路调控大鼠呼吸机相关性肺损伤的机制。方法 40只成年雄性SD大鼠随机分为4组,每组10只,分别为双肺通气组(TLV组),单肺通气组(OLV足),单肺通气+异丙酚组(D组),单肺通气+异丙酚+法舒地尔组(DL组)。对各组大鼠血清血清TNF-α、IL-6及IL-10含量及肺组织RhoA及Rock2蛋白水平和基因表达水平进行测定。结果 D组和DL组大鼠血清TNF-α、IL-6水平低于TLV和OLV组,IL-10水平高于TLV和OLV组,差异有统计学意义(P<0.05)。DL组血清TNF-α、IL-6水平低于D组,IL-10水平高于D组,差异有统计学意义(P<0.05)。D组和DL组大鼠W/D及BALF总蛋白水平低于TLV和OLV组,差异有统计学意义(P<0.05)。D组和DL组大鼠RhoA和Rock2蛋白含量低于TLV和OLV组,差异有统计学意义(P<0.05)。DL组大鼠RhoA和Rock2蛋白含量水平低于D组,差异有统计学意义(P<0.05)。结论异丙酚能够有效缓解呼吸机相关肺损伤的炎性反应,同时其作用机制可能与其能够抑制Rho/Rock信号通路的激活有关。 展开更多
关键词 异丙酚 RHO/rock信号通路 呼吸机相关性肺损伤 大鼠
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Distribution Characteristics of Effective Source Rocks and Their Control on Hydrocarbon Accumulation: A Case Study from the Dongying Sag, Eastern China 被引量:32
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作者 ZHUGuangyou JINQiang +3 位作者 ZHANGShuichang DAIJinxing ZHANGLinye LIJian 《Acta Geologica Sinica(English Edition)》 SCIE CAS CSCD 2004年第6期1275-1288,共14页
The exploration conducted in the Bohai Bay basin, eastern China has demonstrated that the abundant petroleum resources have close affinities to the hydrocarbon kitchen with rich organic matter. A number of oil-generat... The exploration conducted in the Bohai Bay basin, eastern China has demonstrated that the abundant petroleum resources have close affinities to the hydrocarbon kitchen with rich organic matter. A number of oil-generating associations with various characteristics of organic geochemistry and assemblages of multiple reservoir facies are developed due to the multi-center sedimentation, multi-source supply and multi-cycle evolution of filling, which have resulted in the formation of multiple oil and gas accumulation zones of various layers and trap styles. Among them the Paleogene Shahejie Formation is the most important hydrocarbon accumulation combination in the Dongying sag. Heretofore, its proved reserve has reached nearly 1.8×109t, which accounts for more than 90% of the total proved reserves of the Dongying sag. Based on previous studies, more than 600 source rock samples and 186 crude oil samples of the Shahejie Formation, collected from 30 oilfields, have been treated with organic geochemical testing. By combining the sedimentary sequence characteristics and geochemical analysis results, the source rocks of the Shahejie Formation can be divided into three genetic types, i.e. the saline lake facies (the upper ES4, brackish water deep lake facies (the lower ES3) and fresh-water lake facies (the middle ES3), which correspond to the under-filling, balanced-filling and over-filling lake types respectively and represent the three most essential genetic models for source rocks of non-marine fault basins in China. Based on a thorough oil-source correlation, the source rocks of the saline lake and brackish deep lake facies are determined to be the most contributive source rocks in the Dongying sag. Furthermore, by probing into the accumulation of organic matter and hydrocarbon migration pattern, it can be inferred that the hydrocarbons, generated from source rocks of the saline lake facies with relatively low maturity, have mainly accumulated in the uplifted parts of the basin's margin via lateral migration and the reservoirs are dominated by the buried hill and stratigraphic types, while hydrocarbons generated from source rocks of the brackish lake facies are mainly distributed in the basin's center via vertical migration and characterized by forming lithologic reservoirs. Also, the reservoirs located in the transitional belts have complex types and mostly controlled by faults, characterized by block (structural-lithologic) reservoirs, and the crude oils mainly come from source beds of Es31 and Es42 respectively. 展开更多
关键词 source rocks association characteristics fluctuation lacustrine facies sedimentation heterogeneity Dongyingsag
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Rho/ROCK2通路在阿尔茨海默病病理进程中的重要作用 被引量:4
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作者 张天弼 张云龙 +1 位作者 胡警文 张应玖 《国际药学研究杂志》 CAS CSCD 北大核心 2016年第1期33-38,共6页
Rho/Rho相关卷曲螺旋形成蛋白激酶(ROCK)通路是生物体中广泛存在的经典信号通路,参与多种生理调节过程。ROCK有2种亚型,即ROCK1和ROCK2。在阿尔茨海默病(AD)患者脑中,Rho/ROCK2表现为活性上调,并伴有Aβ42水平升高,以及神经细胞突起的... Rho/Rho相关卷曲螺旋形成蛋白激酶(ROCK)通路是生物体中广泛存在的经典信号通路,参与多种生理调节过程。ROCK有2种亚型,即ROCK1和ROCK2。在阿尔茨海默病(AD)患者脑中,Rho/ROCK2表现为活性上调,并伴有Aβ42水平升高,以及神经细胞突起的形态与功能异常,推测AD的发生、发展与Rho或ROCK2的高表达或过度激活有关。目前Rho/ROCK2通路被认为是预防和治疗AD的一个有效的靶通路,而Rho或ROCK2,也成为药物研发的重要靶点。研究发现,降低Rho或ROCK2的表达,或者抑制Rho或ROCK2的活性均可减少Aβ42诱导的神经毒性,保护神经元,减缓AD的发展。因此,Rho/ROCK2的特异性抑制对中枢神经损伤修复及AD的治疗有重要的意义。为此,本文针对Rho/ROCK2通路在阿尔茨海默病发展中的作用做一综述。 展开更多
关键词 RHO Rho相关卷曲螺旋形成蛋白激酶 阿尔茨海默病 抑制剂
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RhoA/ROCK信号通路与心血管系统疾病关系的研究进展 被引量:2
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作者 张丽美 王国强 +2 位作者 陈世娇 许金鹏 宋书江 《医学研究与教育》 CAS 2021年第2期8-13,共6页
心血管疾病是中国人群死亡的主要原因,然而其发病机制尚不明确。研究发现,RhoA/ROCK信号通路与高血压、冠心病、心力衰竭、心律失常等疾病的发生和发展密切相关。ROCK抑制剂也为心血管疾病的治疗提供新的思路,现就RhoA/ROCK信号通路在... 心血管疾病是中国人群死亡的主要原因,然而其发病机制尚不明确。研究发现,RhoA/ROCK信号通路与高血压、冠心病、心力衰竭、心律失常等疾病的发生和发展密切相关。ROCK抑制剂也为心血管疾病的治疗提供新的思路,现就RhoA/ROCK信号通路在心血管疾病中研究做一综述。 展开更多
关键词 RhoA/rock信号通路 心血管疾病 RHO激酶抑制剂
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Dolomite genesis and reservoir-cap rock assemblage in carbonate-evaporite paragenesis system 被引量:2
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作者 HU Anping SHEN Anjiang +4 位作者 YANG Hanxuan ZHANG Jie WANG Xin YANG Liu MENG Shaoxing 《Petroleum Exploration and Development》 2019年第5期969-982,共14页
Regarding to the problem on the reservoir-cap rock assemblage evaluation in the carbonate-evaporite paragenesis system,this study examined the dolomite and reservoirs genesis and the characteristics of reservoir-cap r... Regarding to the problem on the reservoir-cap rock assemblage evaluation in the carbonate-evaporite paragenesis system,this study examined the dolomite and reservoirs genesis and the characteristics of reservoir-cap rock assemblage.Based on the literature research of the global carbonate reservoirs and the case study on four profiles of carbonate-evaporite succession,together with geological and experimental work,three aspects of understandings are achieved.(1)Lithology of carbonate-evaporite paragenesis system is mainly composed of microbial limestone/bioclastic limestone,microbial dolomite,gypsum dolomite and gypsum salt rock deposited sequentially under the climatic conditions from humid to arid,and vice versa,and an abrupt climate change event would lead to the lack of one or more rock types.(2)There developed two kinds of dolomite(precipitation and metasomatism)and three kinds of reservoirs in the carbonate-evaporite system;and the carbon dioxide and organic acid generated during early microorganism degradation and late microbial dolomite pyrolysis process,and early dolomitization are the main factors affecting the development of microbial dolomite reservoirs with good quality.(3)In theory,there are 14 types of reservoir-cap rock assemblages of six categories in the carbonate-evaporite system,but oil and gas discoveries are mainly in four types of reservoir-cap rock assemblages,namely"microbial limestone/bioclastic limestone–microbial dolomite–gypsum dolomite–gypsum salt rock","microbial limestone/bioclastic limestone–gypsum salt rock","microbial dolomite–gypsum dolomite–gypsum salt rock"and"gypsum dolomite–microbial dolomite–tight carbonate or clastic rock".These four kinds of reservoir-cap rock assemblages should be related with the climate change rules in the geologic history,and have good exploration prospects. 展开更多
关键词 carbonate-evaporate PARAGENESIS system lithological association sequence microbial DOLOMITE gypsum DOLOMITE reservoir-cap rock ASSEMBLAGE
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Duobaoshan Porphyry Copper DepositandIts Associated Components
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《Journal of Earth Science》 SCIE CAS CSCD 2000年第2期83-86,共4页
关键词 In BODY rock Duobaoshan Porphyry Copper DepositandIts associated Components
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The Chromitites Associated with the Pan-African Ophiolites in Egypt
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作者 Mohamed Metwaly ABU ANBAR 《Acta Geologica Sinica(English Edition)》 SCIE CAS CSCD 2016年第S1期206-208,共3页
Ophiolites components occur in Pan-African belt in Central Eastern Desert(CED)and South Eastern Desert(SED.The ultramafic components are severely serpentinized and in some areas occur as small fresh
关键词 SED CED The Chromitites associated with the Pan-African Ophiolites in Egypt CR rock high
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