This study was designed to evaluate the bioactivities such asβ-glucosidase activity,α-galactosidase activity,and the growth behavior of the Lactobacillus cultures in soy milk medium.Ten Lactobacillus cultures were c...This study was designed to evaluate the bioactivities such asβ-glucosidase activity,α-galactosidase activity,and the growth behavior of the Lactobacillus cultures in soy milk medium.Ten Lactobacillus cultures were considered in this study.L.fermentum(M2)and L.casei(NK9)were selected due to their betterα-galactosidase,β-glucosidase activity and growth behavior in soy milk medium during fermentation.Further,soy milk fermented with M2 showed higher proteolytic activity(0.67 OD)and ACE-inhibitory(48.44%)than NK9(proteolytic activity:0.48 OD and ACE-inhibitory activity:41.33%).Bioactive peptides produced during the fermentation of soy milk using the selected Lactobacillus cultures were also identified with potent ACE-inhibitory activity by MALDI-TOF spectrometry,and the identified ACE inhibitory peptide sequences from fermented soy milk were characterized using Biopep database.展开更多
文摘This study was designed to evaluate the bioactivities such asβ-glucosidase activity,α-galactosidase activity,and the growth behavior of the Lactobacillus cultures in soy milk medium.Ten Lactobacillus cultures were considered in this study.L.fermentum(M2)and L.casei(NK9)were selected due to their betterα-galactosidase,β-glucosidase activity and growth behavior in soy milk medium during fermentation.Further,soy milk fermented with M2 showed higher proteolytic activity(0.67 OD)and ACE-inhibitory(48.44%)than NK9(proteolytic activity:0.48 OD and ACE-inhibitory activity:41.33%).Bioactive peptides produced during the fermentation of soy milk using the selected Lactobacillus cultures were also identified with potent ACE-inhibitory activity by MALDI-TOF spectrometry,and the identified ACE inhibitory peptide sequences from fermented soy milk were characterized using Biopep database.