In this study,SHECN(Ser-His-Glu-Cys-Asn)and SHCMN(Ser-His-Cys-Met-Asn)soybean peptides were used as the research objects to investigate their antioxidant activities and XO(xanthine oxidase)activity reduction effects.T...In this study,SHECN(Ser-His-Glu-Cys-Asn)and SHCMN(Ser-His-Cys-Met-Asn)soybean peptides were used as the research objects to investigate their antioxidant activities and XO(xanthine oxidase)activity reduction effects.The results show that SHECN and SHCMN have strong antioxidant activities,and at the same time protect the oxidative stress damage caused by HUA(hyperuricemia).They can quench XO,change the secondary structure of XO,and reduce its activity,which leads to the reduction of UA(uric acid)levels.Molecular docking demonstrated soybean peptides could bind to XO target proteins,respectively.In this study,SHECN and SHCMN were obtained from soybean peptides.Based on the data,soy peptides may be an attractive natural ingredient for the development of novel functional foods that inhibit XO activity.展开更多
This study was designed to evaluate the bioactivities such asβ-glucosidase activity,α-galactosidase activity,and the growth behavior of the Lactobacillus cultures in soy milk medium.Ten Lactobacillus cultures were c...This study was designed to evaluate the bioactivities such asβ-glucosidase activity,α-galactosidase activity,and the growth behavior of the Lactobacillus cultures in soy milk medium.Ten Lactobacillus cultures were considered in this study.L.fermentum(M2)and L.casei(NK9)were selected due to their betterα-galactosidase,β-glucosidase activity and growth behavior in soy milk medium during fermentation.Further,soy milk fermented with M2 showed higher proteolytic activity(0.67 OD)and ACE-inhibitory(48.44%)than NK9(proteolytic activity:0.48 OD and ACE-inhibitory activity:41.33%).Bioactive peptides produced during the fermentation of soy milk using the selected Lactobacillus cultures were also identified with potent ACE-inhibitory activity by MALDI-TOF spectrometry,and the identified ACE inhibitory peptide sequences from fermented soy milk were characterized using Biopep database.展开更多
基金This work was supported by the National Key Research and Development Program of China(2017YFD0400504).
文摘In this study,SHECN(Ser-His-Glu-Cys-Asn)and SHCMN(Ser-His-Cys-Met-Asn)soybean peptides were used as the research objects to investigate their antioxidant activities and XO(xanthine oxidase)activity reduction effects.The results show that SHECN and SHCMN have strong antioxidant activities,and at the same time protect the oxidative stress damage caused by HUA(hyperuricemia).They can quench XO,change the secondary structure of XO,and reduce its activity,which leads to the reduction of UA(uric acid)levels.Molecular docking demonstrated soybean peptides could bind to XO target proteins,respectively.In this study,SHECN and SHCMN were obtained from soybean peptides.Based on the data,soy peptides may be an attractive natural ingredient for the development of novel functional foods that inhibit XO activity.
文摘This study was designed to evaluate the bioactivities such asβ-glucosidase activity,α-galactosidase activity,and the growth behavior of the Lactobacillus cultures in soy milk medium.Ten Lactobacillus cultures were considered in this study.L.fermentum(M2)and L.casei(NK9)were selected due to their betterα-galactosidase,β-glucosidase activity and growth behavior in soy milk medium during fermentation.Further,soy milk fermented with M2 showed higher proteolytic activity(0.67 OD)and ACE-inhibitory(48.44%)than NK9(proteolytic activity:0.48 OD and ACE-inhibitory activity:41.33%).Bioactive peptides produced during the fermentation of soy milk using the selected Lactobacillus cultures were also identified with potent ACE-inhibitory activity by MALDI-TOF spectrometry,and the identified ACE inhibitory peptide sequences from fermented soy milk were characterized using Biopep database.
