This paper reports establishment of stable diploid cell lines containing nopaline synthase genes from callus containing T-DNA after 65 generations of successive suspension cultures. The strain C58 Agrobacterium tumefa...This paper reports establishment of stable diploid cell lines containing nopaline synthase genes from callus containing T-DNA after 65 generations of successive suspension cultures. The strain C58 Agrobacterium tumefaciens was used for tumor inducing on variety 'Siyuchuang'. The T-DNA-containing tissue was separated in auxin-free MS medium by strictly screening three times from single cells. The presence of nopaline was identified by paper electrophoresis. The T-DNA-containing polyploid soybean cell lines were obtained at the same time. The nopaline synthase genes from T-DNA were stably integrated and expressed in soybean genome, which can be used in gene engineering with soybean.展开更多
文摘This paper reports establishment of stable diploid cell lines containing nopaline synthase genes from callus containing T-DNA after 65 generations of successive suspension cultures. The strain C58 Agrobacterium tumefaciens was used for tumor inducing on variety 'Siyuchuang'. The T-DNA-containing tissue was separated in auxin-free MS medium by strictly screening three times from single cells. The presence of nopaline was identified by paper electrophoresis. The T-DNA-containing polyploid soybean cell lines were obtained at the same time. The nopaline synthase genes from T-DNA were stably integrated and expressed in soybean genome, which can be used in gene engineering with soybean.
