印度洋深海来源真菌Penicillium sp.SCSIO 06871次级代谢产物研究

本文对印度洋深海来源真菌Penicillium sp.SCSIO 06871的次级代谢产物进行了研究。采用硅胶、Sephadex LH-20凝胶、和反相ODS (octadecylsilyl)柱色谱、及半制备高效液相色谱法(high performance liquid chromatography,HPLC)分离等手段对该菌株的大米固体培养基发酵产物进行分离纯化,结合核磁共振、质谱、旋光等谱学方法对分离得到的化合物进行结构鉴定,共鉴定7个聚酮类化合物(1—7)和1个吲哚生物碱类化合物(8),结构分别为:sydowinin B(1)、mrlactone1(2)、AGIB4(3)、methylcordyolC(4)、diorcinol(5)、11-dehydrosydonicacid(6)、(+)-sydonicacid(7)和indole-3-acetate(8)。抑菌活性实验显示,化合物4,5和8对多株致病细菌均显示出不同程度的抑制活性。同时α-葡萄糖苷酶抑制活性实验显示,化合物5—8均有较强的α-葡萄糖苷酶抑制活性。

一株新型高效聚磷菌Glutamicibacter sp.G2的分离鉴定及除磷特性

聚磷菌(Phosphorus accumulating bacteria,PAOs)在生物除磷系统(EBPR)的强化过程中发挥着重要作用。挖掘新型高效聚磷菌并优化其除磷工艺,对于废水生物除磷技术的发展具有重大意义。文章通过富集驯化、分离纯化,结合MOPS蓝白斑(10×)初筛、Albert异染颗粒复筛等方法从某污水处理厂污泥样品中筛选出一株高效聚磷细菌G2,将其鉴定为盐生谷氨酸杆菌(Glutamicibacter halophytocola),并通过单因素试验探究pH、温度、接种量、初始磷含量,以及水体中的阳离子浓度对菌株G2生长及除磷的影响。实验结果表明,该菌株在初始磷浓度为30 mg/L、pH为8.0、8.0%接种量、32℃的条件下发酵72 h后,磷去除率可达78.79%。同时,添加2.0%的Mg^(2+)可以将菌株G2在人工合成废水中的除磷率提高17.50%。文章所筛选出的盐生谷氨酸杆菌G2在人工合成废水中表现出了良好的除磷效果,有望为废水生物除磷技术提供优质菌种资源。

Study of the Effectiveness of Papaver Sp. Alkaloids as Future Therapeutic Alternatives against Enterococcus Sp. Causing Hospital-Acquired Septicemic Infections

Background and Objective: In recent years, control of Enterococcus sp. It has been proven in the local medical environment to be a cause of acquired septicemia in various age groups, and medical instruments are considered an effective means of transmitting enterococcal septicemia, and catheters are at the forefront in terms of danger. Based on this risk, this study aimed to monitor the spread of Enterococcus sp., which causes blood poisoning acquired from catheters, and to compare its response to antibiotics with that of those isolated from clinical samples in children, as a first study locally. The effectiveness of alkaloids of different types of Papaver sp. In Syrian plants, they were tested against infection with this bacteria. Materials and Methods: The study dealt with two parts: The first part included collecting clinical samples from the University Children’s Hospital in Damascus/bacterial diagnostic laboratories/then isolating and diagnosing the bacteria by following a set of tests to identify the most prevalent genera and species and comparing their prevalence rate with Enterococcus. The second part;It included collecting plant samples, confirming the species taxonomically, then extracting alkaloids from plant parts (fruit, stem, Flowers), then comparing the extent of resistance of bacterial strains to antibiotics compared to the Enterococcus sp., and then confirming the antibacterial activity of the Papaver sp. alkaloids against Enterococcus sp. Result:In its first part, the study confirmed the significant contribution of the Enterococcus sp. to infections acquired from various sources, largely in catheter tip infections (9.09%) and to a lesser extent in other sources (3.7%), The second part was to confirm the effective-ness of the alkaloid extract of the Papaver sp., especially the two species Papaver syriacum, and Papaver dubium, against Enterococcus sp. with areole diameters that ranged between (15 - 26 mm) for the fruit extract and at a minimum inhibitory concentration (3.12 - 6.25 mml) and then the stem (5 - 20 mm). And the effectiveness of the Flowers extract is very weak to almost non-existent. Conclusions: The catheter and medical sources surrounding the patient constitute a dangerous source of multi-resistant Enterococcus sp., which poses a real threat to the lives of children, with new mechanisms represented by colonization of the skin and the ability to form biofilms Surfaces of medical instruments, with are resistant to a wide range of antibiotics. As an alternative and effective modern source to limit its spread in the future, the alkaloid extract of the fruits and stems of the wild Papaver sp. has proven a strong antibiotic effect, especially the two types: Papaver syriacum and Papaver dubium.

海洋真菌Phaeosphaeriopsis sp. ZYX-Z-811次生代谢产物研究

对海洋真菌Phaeosphaeriopsis sp. ZYX-Z-811发酵液中次生代谢产物进行分离鉴定,并评价其生物活性。采用正相硅胶柱色谱、反向C18柱色谱、高效液相色谱、SephadexLH-20凝胶柱色谱等技术进行分离纯化,共分离得到10个化合物,利用核磁及红外和紫外波谱技术对分离的单体化合物进行结构鉴定,分别为4-epi-lignicol(1)、lignicol(2)、6-demethylkigelin(3)、6,8-dihydroxy-3,4,7-trimethylisocoumarin(4)、6,7-dimethoxymellein(5)、musaone A(6)、2-(4-hydroxyphenyl)ethylacetate(7)、3-吲哚甲醛(8)、吲哚-3-乙酸甲酯(9)、对羟基苯甲酸甲酯(10),其中化合物1为未发表的化合物,文章通过电子圆二色谱(electrostatic circular dichroism, ECD)计算确定了其绝对构型。对这些化合物分别进行抗氧化和α-糖苷酶抑制活性评价,发现化合物10有弱的抗氧化活性。

无花果内生菌Alternaria sp.QDFB-2菌丝体化学成分的研究

为研究无花果枝条内生真菌Alternaria sp.QDFB-2菌丝体的化学成分,采用硅胶柱层析、Sephadex LH-20凝胶柱层析、反相柱层析和高效液相色谱等方法对Alternaria sp.QDFB-2菌丝体粗浸膏进行分离纯化,利用核磁共振谱(NMR)和质谱(MS)等现代波谱技术、结合文献比对方法确定化合物的结构;采用MTT法进行化合物的体外细胞毒活性评价。结果表明:从Alternaria sp.QDFB-2菌丝体粗浸膏中分离纯化得到7种化合物:alternariol(1)、tentoxin(2)、dihydrotentoxin(3)、cyclo(Gly-Pro)(4)、cyclo(4-hydroxy-Pro-Pro)(5)、cyclo(S-Leu-S-N-methyl-Phe)(6)和uridine(7)。体外细胞毒活性评价显示,化合物1和7对SMMC-7721细胞具有中等增殖抑制活性,其IC50值分别为50.47μg·mL^(-1)(195.62μmol·L^(-1))和42.50μg·mL^(-1)(174.18μmol·L^(-1));化合物1对A549细胞也具有中等的增殖抑制活性,浓度为100μg·mL^(-1)时抑制率为52.12%;阳性对照顺铂的IC50值分别为3.46μg·mL^(-1)(11.53μmol·L^(-1))和5.12μg·mL^(-1)(17.06μmol·L^(-1))。Alternaria sp.QDFB-2菌丝体的化学成分丰富,值得深入研究。

西沙马尾藻共附生真菌Aspergillus sp.SYPUF41304次级代谢产物的分离与鉴定

目的对西沙马尾藻共附生真菌Aspergillus sp.SYPUF41304的次级代谢产物进行分离与鉴定。方法利用硅胶柱色谱、凝胶柱色谱、中压反相柱色谱及半制备高效液相色谱等分离手段对马尾藻共附生真菌Aspergillus sp.SYPUF41304的液体发酵提取物进行分离纯化,并利用核磁共振、质谱等波谱学方法,结合文献对照,确定化合物结构。结果最终从提取物中分离得到10个化合物,经鉴定为吲哚-3-甲醛(1H-indole-3-carboxaldehyde)(1)、吲哚-3-乙酸甲酯(methyl indole-3-acetate)(2)、环(L-脯氨酸-L-缬氨酸)(cyclo-L-prolyl-L-valine)(3)、brevianamide F(4)、asperterreusine A(5)、talaisocoumarin A(6)、3-甲基-6-羟基-8甲氧基-3,4-二氢异香豆素(3-methyl-6-hydroxy-8-methoxy-3,4-dihydroisocoumarin)(7)、3-甲氧基-6,8-二羟基-3-甲基-3,4-二氢异香豆素(3-methoxy-6,8-dihydroxy-3-methyl-3,4-dihydroisocoumarin)(8)、4-hydroxykigelin(9)和de-O-methyldiaporthin(10)。结论化合物按结构类型可分为生物碱和聚酮类等,化合物4~8均为首次从该属真菌中分离得到。所有化合物在4 mg·mL^(-1)浓度下,均未显示出抗细菌活性。

Serratia sp.对铜绿微囊藻、斜生栅藻的溶藻效果

【目的】探讨一株溶藻菌S6(Serratia sp.)对富营养化水中优势蓝藻和绿藻的溶藻效果,为有害藻类的生物控制提供参考。【方法】从富营养化水体中分离到一株溶藻菌S6(Serratia sp.,GenBank登录号为KY462187),将不同浓度菌液分别加至铜绿微囊藻(Microcystis aeruginosa)、斜生栅藻(Scenedesmus obliquus)以及两者以浓度比1∶1混合的藻液(下称“混合藻”)中,进行溶藻实验,测定14 d内实验体系中藻的生物量、叶绿素a、氨氮和总磷(TP)浓度,分析溶藻菌S6对不同藻类的溶藻效果以及对水体中氮磷的影响;运用光谱技术分析溶藻产物特性。【结果与结论】溶藻菌S6对铜绿微囊藻、斜生栅藻和混合藻的溶藻效果较佳,且菌液浓度越高,溶藻效果越佳,其中3组原菌液(2×10^(9)mL^(-1))处理组14 d内的溶藻率分别为89.4%、80%、78.6%。溶藻菌S6对3种实验体系氨氮和TP浓度影响较大,原菌液处理组变化最明显,其中铜绿微囊藻藻液氨氮和TP质量浓度分别下降21.24、6.21 mg/L;斜生栅藻藻液氨氮和TP质量浓度分别下降13.19、7.54 mg/L;混合藻液中氨氮和TP质量浓度分别下降10.81、11.85 mg/L。傅里叶红外光谱、紫外光谱和三维荧光光谱分析表明,铜绿微囊藻的溶藻产物中主要含类色氨酸、类富里酸和类腐殖酸物质,斜生栅藻的溶藻产物中主要含类色氨酸和类腐殖酸物质。

金刚纂内生真菌Irpex sp.的次生代谢产物研究

通过硅胶柱层析和半制备型高效液相色谱等分析方法对金刚纂(Euphorbia neriifolia L.)内生真菌Irpex sp.的液体发酵产物进行提取与分离,共得到9个化合物,分别为IrpexIacteC(1)、IrpexIacteD(2)、5-(4-Oxopentyl)furan-2-carbaldehyde(3)、22-tetraen-3-one(4)、IrlactinL(5)、IrlactinK(6)、IrlactinI(7)、Davotremulane B(8)、(+)-(1R,6S,7S)-Tremul-2-ene-12(11)-lactone(9),其中,3个为呋喃衍生物,5个为倍半萜化合物,1个为甾醇类化合物;化合物3,4,8,9为首次从耙齿菌属Irpex sp.中分离得到.

两种磁性生物炭耦合Diaphorobacter sp.DFA4去除2,4-DFA和Cu^(2+)的特性

以橘子皮为原料,氯化铁、镍锰铁氧体为磁性介质,制备了氯化铁和镍锰铁氧体改性生物炭(分别记为FBC和MBC),重点考察FBC和MBC介导Diaphorobacter sp.DFA4耦合体系去除2,4-二氟苯胺(2,4-DFA)和Cu^(2+)的特性。结果表明:在0.5~2.0 g/L FBC/MBC的介导作用下,Diaphorobacter sp.DFA4对2,4-DFA和Cu^(2+)的去除效果明显提升。当2,4-DFA初始质量浓度为100~900 mg/L时,相比纯菌Diaphorobacter sp.DFA4培养体系,FBC和MBC介导耦合体系对2,4-DFA的降解强化倍数分别为2.24~4.04倍和1.96~4.71倍,Cu^(2+)去除率则分别由59.70%~69.95%提升至88.57%~93.20%和82.12%~88.20%。当pH为6.0~8.0时,较纯菌Diaphorobacter sp.DFA4培养体系,MBC、FBC介导耦合体系对2,4-DFA的去除率分别提高了15.35%~36.32%和22.43%~37.42%,对Cu^(2+)的去除率则分别提高了37.63%~42.34%和43.61%~47.83%;当盐度由0提高至4%时,两个耦合体系对2,4-DFA的去除率基本保持在85%及以上,而纯菌Diaphorobacter sp.DFA4培养体系对2,4-DFA的去除率则由77.98%降至55.19%。机理分析发现,耦合体系可诱导分泌更多的腐殖酸,且在偏碱条件下,FBC更有利于促使腐殖酸的分泌。

副球菌(Paracoccus sp.)提取物对米氏凯伦藻(Karenia mikimotoi)的抑制作用

该文研究了副球菌O-4对米氏凯伦藻的抑藻活性、抑藻途径以及抑藻物质.结果表明,菌株O-4主要通过使藻细胞絮凝、细胞破裂和光合系统损伤实现对米氏凯伦藻生长繁殖的抑制,十九碳烯酸(顺-10)是其产生的主要抑藻活性物质,丰富了对抑藻微生物及其抑藻物质的研究,为微生物防治藻华提供了理论数据.

中国南海豆荚软珊瑚Lobophytum sp.的化学成分及其抗菌活性研究

对采集自中国南海的豆荚软珊瑚Lobophytum sp.的化学成分及生物活性进行研究。采用硅胶柱色谱、凝胶柱色谱、半制备高效液相色谱等分离手段进行分离纯化,通过对1H NMR、13C NMR等波谱数据分析并对比相关参考文献数据,确定了化合物的结构。从豆荚软珊瑚Lobophytum sp.中共分离鉴定了12个化合物,包括9个二萜类化合物:(+)-(7R,8R)-epoxy-7,8-dihydrocembrene C(1)、(1R,2E,4R,7E,11E)-cembra-2,7,11-triene-4-ol(2)、(-)-(2R,7R,8R)-sarcophytoxide(3)、entsarcophine(4)、sarcotenusene C(5)、(7S,8S)-epoxy-1,3,11-cembratrien-16-oic methyl ester(6)、sarcophytonin B(7)、sarcoconvolutum D(8)、laevigatol A(9);3个甾体类化合物:菜油甾醇(10)、(24S)-24-甲基胆甾醇(11)、柳珊瑚甾醇(12)。化合物8为首次从豆荚软珊瑚Lobophytum sp.中分离得到。化合物3、4、5、7、10对鱼类致病菌美人鱼发光杆菌FP2244、耐土霉素的副乳房链球菌SPOF3K,以及人类致病菌耐万古霉素屎肠球菌均具有显著的抗菌活性。

Screening and pilot-scale evaluation of a highly efficient pesticide-degrading Pseudomonas sp.strain BL5

The widespread use of pesticides has caused serious harm to ecosystems,necessitating effective and environmentally friendly treatment methods.Bioremediation stands out as a promising approach for pollutant treatment,wherein the metabolic activities of microorganisms can transform toxic pesticides into compounds with lower or no toxicity.In this study,we obtained eight pesticide-degrading strains from pesticide-contaminated sites through continuous enrichment and screening.Four highly efficient pesticide-degrading strains(degradation ratios exceeding 80%)were identified.Among them,Pseudomonas sp.BL5 exhibited the strongest growth(exceeding 10^(9) CFU·ml^(-1))and outstanding degradation of benzene derivatives and chlorinated hydrocarbons at both laboratory and pilot scales,with degradation ratios exceeding 98%and 99.6%,respectively.This research provides new tools and insights for the bioremediation of pesticide-related pollutants.

耐铬Serratia sp. CM01基因组序列及铬代谢基因分析

以耐铬(VI)菌株Serratia sp. CM01为研究对象,探究其全基因组信息,挖掘其潜在的铬代谢相关基因。本研究采用基因组测序技术对CM01进行全基因组测序并分析其基因序列特征;同时,结合前期差异蛋白研究结果,进行铬代谢相关基因分析。测序结果表明,CM01基因组大小为4,902,254 bp,预测编码蛋白序列的基因有4547个;蛋白功能注释结果显示其涉及氧化还原、氨基酸代谢、碳水化合物和能量代谢编码的基因有较高的占比。结合前期蛋白组学的结果,筛选出了12个与铬代谢相关的基因。qRT-PCR分析结果显示,在Cr(VI)胁迫下,ChrA1、Srpc、GrxA和NemA基因的表达上调。CM01基因组全序列已上传至NCBI,序列号:PRJNA675313。本研究通过对CM01的基因组序列分析,为全面了解细菌的铬代谢机制提供基础,为修复环境铬污染的新生物技术提供理论依据。The study focused on the chromium (VI)-resistant bacterial strain Serratia sp. CM01 to explore its whole-genome information and to mine its potential chromium metabolism-related genes. In this research, genomic sequencing technology was employed to perform whole-genome sequencing on CM01 and to analyze its gene sequence characteristics. Additionally, the results from previous differential protein studies were integrated to analyze genes related to chromium metabolism. The sequencing results indicated that the CM01 genome is 4,902,254 base pairs in size, with 4547 genes predicted to encode protein sequences. Protein function annotation revealed a high proportion of genes involved in oxidation-reduction, amino acid metabolism, carbohydrate metabolism, and energy metabolism. Combining the outcomes from previous proteomics studies, 12 genes related to chromium metabolism were identified. Quantitative real-time PCR analysis showed that the expression of ChrA1, Srpc, GrxA, and NemA genes was upregulated under Cr(VI) stress. The complete genome sequence of CM01 has been uploaded to NCBI with the accession number PRJNA675313. Through the genomic sequence analysis of CM01, this study provides a foundation for a comprehensive understanding of bacterial chromium metabolism mechanisms and offers a theoretical basis for the development of new biotechnologies for the remediation of environmental chromium pollution.

霍山石斛内生真菌Trichoderma sp.次生代谢产物研究

目的研究霍山石斛内生真菌Trichoderma sp.的次生代谢产物。方法采用正相硅胶、葡聚糖凝胶、反相C-18及制备液相结合二极管阵列检测器等多种色谱技术分离纯化,所获得的化合物经紫外光谱、核磁共振波谱和质谱数据鉴定。结果从霍山石斛内生真菌Trichoderma sp.中定向分离并鉴定了1个新化合物及8个色原酮和蒽醌类化合物,分别为trichphenoid(1),2,3-dialkylchromone(2),5-hydroxy-2,3-dimethyl-7-methoxychromone(3),7-hydroxy-2-(2'-hydroxypropyl)-5-methylchromone(4),2,5-二甲基-7-羟基色酮(5),1,7-dihydroxy-3-hydroxymethyl-9,10-anthraquinone(6),1-hydroxy-3-hydroxymethyl-9,10-anthraquinone(7),pachybasin(8),大黄酚(9)。结论所有化合物均为首次从霍山石斛内生真菌Trichoderma sp.中分离得到,化合物1为新化合物。

Yr5-virulent races of Puccinia striiformis f.sp.tritici possess relative parasitic fitness higher than current main predominant races and potential risk

Wheat stripe rust,caused by Puccinia striiformis f.sp.tritici(Pst),is one of the most destructive fungal diseases of wheat,and seriously threatens safe production of the crop worldwide.In China,new races historically appeared and rapidly developed to be predominant races and have resulted in ineffectiveness and replacement of wheat resistance cultivars as well as massive reduction in yield.In the present study,the relative parasitic fitness of the two newlyemerged Yr5-virulent races(TSA-6 and TSA-9)were compared with those of four currently predominant Chinese races(CYR31,CYR32,CYR33,and CYR34)based on evaluation on 10 Chinese wheat cultivars.As a result,there were significant differences in the relative parasitic fitness parameters among overall tested races based on multiple comparison(LSD)analysis(P<0.05).The principal component analysis(PCA)of overall parasitic fitness parameters indicated that the sporulation ability,infection and spore survivability,expansion capacity,and potential pathogenicity were the most important parasitic fitness attributes of the tested races.Based on the establishment of extracted three principal components and a comprehensive factor score mathematical models,evaluations of the parasitic fitness attributes of tested races showed that the level of relative parasitic fitness of the tested six races was:CYR32(1.15)>TSA-9(0.95)>TSA-6(0.92)>CYR34(0.29)>CYR31(–1.54)>CYR33(–1.77).The results indicated that two Yr5-virulent races TSA-9 and TSA-6 possessed relative parasitic fitness higher than races CYR34,CYR31,and CYR33,but lower than race CYR32,and have potential risks in developing to be predominant races.Therefore,continual monitoring of both Yr5-virulent races,and their variants is needed.The use of wheat cultivars(lines)with Yr5 resistance gene singly in wheat breeding is essential for being avoided,and is suggested to combine with other effective stripe rust resistance genes.

Bioactive chemical constituents from the marine-derived fungus Cladosporium sp.DLT-5

A new isochromanone,cladosporinisochromanone(1),accompanied by 15 known compounds(2–16)were obtained from secondary metabolites produced by marine-derived fungus Cladosporium sp.DLT-5.NMR and HRESIMS spectra elucidation determined the planar structure of 1.Subsequent electronic circular dichroism(ECD)experiment assigned the absolute configuration of 1.Compounds 1,2,4–6,and 10 displayed different degrees of neuroprotective activities on human neuroblastoma cells SH-SY5Y.Five compounds(1,3–5,and 13)emerged resistance to protein tyrosine phosphatase 1B(PTP1B),further kinetic analysis and molecular docking study indicated that the most potent compound 13(IC50value of 10.74±0.61μmol/L)was found as a noncompetitive inhibitor for PTP1B.Surface plasmon resonance(SPR)and molecular docking studies also demonstrated the interaction between compound 12 and Niemann-Pick C1 Like 1(NPC1L1),which has been identified as significant therapeutic target for hypercholesteremia.In addition,compounds 3,6,and 14 showed attractive inhibitory activity against the phytopathogenic fungi:Colletotrichum capsici.Therefore,library of Cladosporium metabolites is enriched and new active uses of known compounds are explored.

Effects of Fungi Fusarium sp. to Rhizosphere Soil and Physiological Characteristics of Camellia oleifera Abel.

[Objectives]To study the effects of fungi Fusarium sp.to rhizosphere soil and physiological characteristics of Camellia oleifera Abel.[Methods]We investigated the effects of Fusarium sp.to rhizosphere soil nutrient element content and metabolites of C.oleifera.C.oleifera was inoculated with the suspension of Fusarium sp.in pot experiments and ammonium-N,available phosphorus,available potassi-um,organic matter,enzymes and pH of rhizosphere soil,MDA content,activity of SOD,POD of C.oleifera leaves were analyzed.[Results]Fusarium sp.stress significantly inhibited soil enzyme activities and significantly reduced available phosphorus content,especially for phospha-tase and sucrase.Antioxidant enzyme activities in C.oleifera tissues showed that Fusarium sp.stress significantly increased MDA and SOD enzyme activities and decreased POD enzyme activity.Especially,SOD enzyme activity was elevated by 53.86%compared with the CK group.In addition,analysis of the content of major metabolites in C.oleifera leaves showed that Fusarium sp.stress significantly reduced the content of total flavonoids,quercetin,isoquercitrin and isoquercitrin in C.oleifera leaves by 7.80%,50.00%and 75.90%,respectively.[Conclusions]Our results are an important step which showed strong resistance of C.oleifera and can give a novel insight for researches on the effects in the rhizosphere soil enzyme,soil nutrient elements and metabolites of C.oleifera under the Fusarium sp.too.

Arthrobacter sp. AD30和Pseudomonas sp. AD39在阿特拉津工业废水生物处理及污染土壤生物修复中的应用

将分类地位和降解特性不同的两个高效降解除草剂阿特拉津的菌株Arthrobacter sp.AD30和Pseudo-monas sp.AD39,用于阿特拉津工业废水的生物处理和污染土壤的生物修复试验.填充聚氨酯泡沫的小型生物反应器阿特拉津降解实验表明,在进水的CODCr为1702mg.l-1、阿特拉津浓度为133mg.l-1和水力停留时间(HRT)为24h的条件下,出水的CODCr稳定在100mg.l-1以下,阿特拉津浓度在0.2mg.l-1以下,均达到国家工业水污染物排放标准(GB21523—2008).土壤的生物修复实验表明,含有200mg·kg-1阿特拉津的土壤接种上述两个菌株,在30℃处理20d以后,土壤中99.1%的阿特拉津被去除.这些结果表明,由AD30和AD39组成的混合菌株在工业废水的生物处理和污染土壤的生物修复中具有很好的应用潜力.

固定化Acinetobacter sp. XA05和Sphingomonas sp. FG03降解苯酚

从活性污泥和受苯酚污染的土壤中分离出的菌株XA05和FG03均具有很强的苯酚生物降解能力。16srDNA序列分析表明,XA05和FG03菌株分别属于不动杆菌属(Acinetobacter sp.)和鞘氨醇单胞菌属(Sphin-gomonas sp.)。实验结果表明,在苯酚初始质量浓度为800.0mg/L、培养时间为35h的条件下,自由悬浮细胞和固定化细胞的苯酚降解率均高于95.0%。

金枪鱼共附生菌Streptomyces sp.TUN21的次级代谢产物研究

海洋动物共附生微生物次级代谢产物是海洋药物的重要来源。该文从金枪鱼共附生菌Streptomyces sp.TUN21的次级代谢产物中分离得到3个化合物,分别为campechic acid B(1),5-(3’-aminophenyl)-2,4-pentadienoic acid(2)和benzenemethanol(3)。其中化合物1对PC-3和HCT-116的抑制活性的IC_(50)值分别为(1.3±0.2)μmol和(0.64±0.1)μmol,而化合物2是首次从该属中分离得到的天然产物。化合物1~3在50μg/mL的浓度下对耐甲氧金黄色葡萄球菌、大肠杆菌和白色念珠菌无抑制活性。