Tacrolimus Inhibits Vasoconstriction by Increasing Ca^(2+) Sparks in Rat Aorta

The present study attempted to test a novel hypothesis that Ca^2＋ sparks play an important role in arterial relaxation induced by tacrolimus. Recorded with confocal laser scanning microscopy, tacrolimus（10 μmol/L） increased the frequency of Ca^2＋ sparks, which could be reversed by ryanodine（10 μmol/L）. Electrophysiological experiments revealed that tacrolimus（10 μmol/L） increased the large-conductance Ca^2＋-activated K＋ currents（BKCa） in rat aortic vascular smooth muscle cells（AVSMCs）, which could be blocked by ryanodine（10 μmol/L）. Furthermore, tacrolimus（10 and 50 μmol/L） reduced the contractile force induced by norepinephrine（NE） or KCl in aortic vascular smooth muscle in a concentration-dependent manner, which could be also significantly attenuated by iberiotoxin（100 nmol/L） and ryanodine（10 μmol/L） respectively. In conclusion, tacrolimus could indirectly activate BKCa currents by increasing Ca^2＋ sparks released from ryanodine receptors, which inhibited the NE- or KCl-induced contraction in rat aorta.

The Nobel Literature Prize Sparks a Mo Yan Craze

正At 19 o'clock, October 11th, Beijing time, the Swedish Academy awarded the 2012 Nobel Prize for Literature to the Chinese writer Mo Yan. Within several hours, Mo Yan's works were struck by the "Nobel effect", sales shooting up in both bookstores and online marts. Some websites also jumped on the bandwagon and put up a reservation service for the new editions.

Watch the Sparks Fly in Yuxian!

yUXIAN in northwestern Hebei Province is a three-hour drive from Beijing. A prefecture capital as far back as the 6th century, this rural town sits on a wealth of heritage from its long past, including over 580 ancient buildings, nine of which are under national protection. Besides these tangible monuments of wood and bricks, the area, thanks to its seclusion and detachment from an industrial economy, has kept alive the old farmingbased culture and customs of China. Around Spring Festival time every year residents from nearby cities flock to join local celebrations in Yuxian, eager to experience a long lost connection with the land.

Dog Meat Festival Sparks Controversy

A controversial annual dog meat festival is celebrated on summer solstice, falling on June 21 or 22, in Yulin, a city in south China＇s Guangxi Zhuang Autonomous Region. However, the festival has drawn widespread criticism in recent years.

Experimental Study on Methane Explosion Ignited by Sparks of Cable Bolt Breakage

An experimental device was designed for studying methane explosion ignited by sparks of cable bolt breakage. With the methane concentration being in explosion range, a series of experiments were conducted to study the law of spark generation during cable bolt breakage and the probability of methane explosion caused by the spark. The results show that the probability of generating sparks during cable bolt breakage is 50%. The spark generated by the breakage of steel cable bolt strand can't ignite a methane explosion. A detection was carried out using infrared-ray imaging apparatus (IRIA) to measure temperature of the spark generated by cable bolt breakage. It is indicated that the maximum temperature of the spark generated by cable bolt breakage is far less than the required ignition temperature for a methane explosion.

The Optical Diagnosis of Underwater Positive Sparks and Corona Discharges

In this paper, two types of underwater discharges, spark discharge and corona discharge, are investigated by optical diagnosis using a high speed framing camera （HSFC） with the framing time within nanoseconds under the same experimental conditions. In order to capture the photographs of streamer propagation, the influence of the randomicity of the prebreakdown duration is taken into consideration. By increasing the conductivity of water, the randomicity reduces effectively. Experimental results show that, for a spark discharge, the process can be separated into three stages： the generation and propagation of a streamer, the generation and expansion of the discharge channel, and the development and annihilation of the plasma. The streamers do not directly move to the opposite electrode, but form a bush-like figure. With the increase of the number of branches, the velocity of streamer propagation slows down. The trajectory of the initial channel between electrodes is not straight. However, with the channel expanding, its shape transforms into a straight column. For a corona discharge, there are two stages： the generation and propagation of a streamer, and the stagnation and annihilation of the streamer. The initial streamer in a corona discharge is generated later than in a spark discharge. The forms of streamers for both kinds of discharge are similar; however, streamers generated by a corona discharge propagate with a slower velocity and the number of branches is less compared with a spark discharge. When the energy injection stops, the luminescence of plasma inside the discharge channel （spark discharge） or streamers （corona discharge） becomes weaker and weaker, and finally disappears.

Repeated Sparks Produce Free Energy: A Rigorous Mathematical Explanation of the Authors, by the Hidden Ampere Force Law

This is the twinned paper of the increased mass paper, “Gravity is acting at superluminous infinite speed, thus collapsing special theory of relativity of Einstein, an explanation for almost everything (according to the authors’ rigorous mathematical proof and overwhelming experimental evidence)"?[1]. In the present paper, we describe the repeated spark or repeat current in terms of the hidden, Non-Relativistic Longitudinal Cardinal Ampere Force which is not found yet infallible for every case. The impressive finding is the fact that these repeated sparks as we shall see, have no back-emf, but forward-emf and forward-Ampere-motive-force. The only consumption is the resistive load with the very high currents needed. This means its total energy consumption is less than the released energy-output work plus heat, plus the energy recharging the batteries. All these are in excess of the consumed input energy. This means the Repeated sparking is “over-unity” efficiency and that, using super-conductive materials, we shall have constructed the first “perpetual mobile” plus producing extra electrical energy. Several previous journals that we did not mention here their names did not dare to publish our disliked papers of very important and revolutionary results but were not able to counter argue our arguments. They preferred the silence and have not answered us at all for many years now. Even some of them were involved previously with the repeated sparking which is the same topic of our present paper.

Learning Chinese Sparks Passion for Life

“One World,One Family”is a theme that conjures up images of harmony and the desire to bridge cultural divides,so it was a fitting backdrop for the 18th“Chinese Bridge”—Chinese Proficiency Competition for Foreign College Students held in Changsha,Hunan Province this year.

失物招领处:寻找1‰秒的Soul Sparks

我是一名外科医生,今年30岁。30岁生日那天我在微博上写了一篇文章,题目是《你还记得那个青年医生吗?》。孔老夫子所说的"三十而立",我们究竟应该如何理解呢?是发一篇牛气闪闪的SCI吗,还是申请一项超过百万级别的科研基金呢,还是独立操作达芬奇机器人做一台手术呢,或者,我们换一栋大房子,买一辆心仪已久的跑车,还是带着心爱的姑娘去周游世界?像我们这样,30岁上下,夹在现实与理想之间,父母和孩子之间。

Sparks of the CRISPR explosion:Applications in medicine and agriculture

Exactly 30 years ago,Nakata and coworkers published a paper analyzing the 1664-nucleotide sequence of a chromosomal DNA segment that contained the iap gene and its flanking regions in Escherichia coli（Ishino et al.,1987）.

Contribution of spontaneous L-type Ca^(2+) channel activation to the genesis of Ca^(2+) sparks in resting cardiac myocytes

Ca2+ sparks are the elementary events of intracellular Ca2+ release from the sar-coplasmic reticulum in cardiac myocytes. In order to investigate whether spontaneous L-type Ca2+ channel activation contributes to the genesis of spontaneous Ca2+ sparks, we used confocal laser scanning microscopy and fluo-4 to visualize local Ca2+ sparks in intact rat ventricular myocytes. In the presence of 0.2 mmol/L CdCI2 which inhibits spontaneous L-type Ca2+ channel activation, the rate of occurrence of spontaneous Ca2+ sparks was halved from 4.20 to 2.04 events/(100 μm·s), with temporal and spatial properties of individual Ca2+ sparks unchanged. Analysis of the Cd2+-sensitive spark production revealed an open probability of-10-5 for L-type channels at the rest membrane potentials (-80 mV). Thus, infrequent and stochastic openings of sarcolemmal L-type Ca2+ channels in resting heart cells contribute significantly to the production of spontaneous Ca2+ sparks.

李希奎秘书长出席缘梦Sparks大学生公益青年营分享会

7月25日,李希奎秘书长在京出席缘梦Sparks大学生公益青年营分享会。李希奎秘书长向参加活动的大学生介绍了我会、基金会的有关情况,并勉励大家做'有爱心、有道德、有抱负的人',通过青年营增长才干,为中国汽车工业的进一步发展做出自己的贡献。缘梦Sparks大学生公益青年营是由基金会'汽车人发展基金'资助开展。

Sexually Frustrated Dolphin Sparks Alert

孔夫子的“饮食男女”一说,看来已经超出了人类的范畴。文章涉及的dolphin(海豚)简直令人难以置信！特别是以下一句: When dolphins get sexually excited,they try to isolate a swimmer,normallyfemale.They do this by circling around the individual and gradually move themaway from the beach,boat or crowd of people. 译文:当海豚处于性激动时,它们会孤立一个游泳者,通常是女性。它们围绕个别的游泳者打圈,慢慢地将这些人从海滩上、船上或是人群中引开去。

5-Hydroxytryptamino-induced calcium sparks in cultured rat stomach fundus smooth muscle cells

With a new fluorescence probe of Ca2+, STDIn-AM, 5-hydroxytryptamino (5-HT)-induced spontaneous calcium release events (calcium sparks) in cultured rat stomach fundus smooth mus-cle cells (SFSMC) are investigated by laser scanning confocal microscope. The mechanisms of initiation of Ca2+ sparks, propagating Ca2+ waves and their relation to E-C coupling are discussed. After the extracellular [Ca2+] is increased to 10 mmol/L, addition of 5-HT causes hot spots throughout the cytoplasm, which is brighter near the plasmalemma. The amplitude of the event is at least two times greater than the standard deviation of fluorescence intensity fluctuations meas-ured in the neighboring region and the duration of the Ca2+ signal is over 100 ms. The results sug-gest that 5-HT acts by the way of 5-HT2 receptors on SFSMC, then through 5-HT2 receptors cou-ples IP3/Ca2+ and DG/PKC double signal transduction pathways to cause Ca2+ release from intra-cellular Ca2+ stores and followed Ca2+ influx possibly through calcium release-activated calcium influx. The acceptor of activated 5-HT2 can also cause membrane depolarization, which then stimulates the L-type Ca2+ channels leading to Ca2+ influx. Then the local Ca2+ entry mentioned above activates ryanodine-sensitive Ca2+ release channels (RyR) on sarcoplasmic reticulum (SR) to cause local Ca2+ release events (Ca2+ sparks) through calcium-induced calcium release (CICR).

Modulating sparks in a pulse train for repetitive and energy efficient plasma generation

Spark is a widely studied plasma source for active species production;however,it ex-periences unstable transitions(e.g.to a thermal arc)at high frequencies or long pulse durations.In this study,the sparks generated in a pulse train were studied and modulated based on a physics‐corrected deep learning method.Our results show that a highly repeatable and stable spark plasma source can be achieved by automatically adjusting the voltage amplitude according to the discharge frequency in a high‐frequency pulse train within the time scale of the fluid response.The influences of the electron number density increasing mode and modulated driven voltage profiles on the energy efficiencies were also studied.

医学影像学教学模式的现状与实践

医学影像学是临床医学中一门实践性极强辅助学科,同时也是发展迅猛,日新月异的学科,在现代医疗中扮演着越来越重要的角色。文章通过对影像医学教学的基本要求、教学目标和目的进行说明,采用影像存储和传输系统(picture archiving and communication system,PACS)结合以问题为基础的教学法(problem-based learning,PBL)及以案例为基础的教学法(case-based learning,CBL)教学法进行实践。针对非影像专业学员学习时间短、学习任务重,学员影像基础和需求迥异,部分非影像专业学员自身对医学影像学的重视不足以及放射基地重视不足等问题。文章阐述了PACS与PBL、CBL教学法、钉钉、微信及SPARK学习平台等学习沟通平台相结合,并增加专职人员对基地管理、带教老师一对一指导,以及不断加强科室建设、安排名师上课来提高科室影响力等多种混合教学模式的解决对策,来唤起学员学习影像相关知识的内驱力,达到提高学员轮转学习效果的目的。

基于互信息和融合加权的并行深度森林算法

针对大数据环境下并行深度森林算法中存在不相关及冗余特征过多、多粒度扫描不平衡、分类性能不足以及并行化效率低等问题,提出了基于互信息和融合加权的并行深度森林算法(parallel deep forest algorithm based on mutual information and mixed weighting,PDF-MIMW)。首先,在特征降维阶段提出了基于互信息的特征提取策略(feature extraction strategy based on mutual information,FE-MI),结合特征重要性、交互性和冗余性度量过滤原始特征,剔除过多的不相关和冗余特征;接着,在多粒度扫描阶段提出了基于填充的改进多粒度扫描策略(improved multi-granularity scanning strategy based on padding,IMGS-P),对精简后的特征进行填充并对窗口扫描后的子序列进行随机采样,保证多粒度扫描的平衡;其次,在级联森林构建阶段提出了并行子森林构建策略(sub-forest construction strategy based on mixed weighting,SFC-MW),结合Spark框架并行构建加权子森林,提升模型的分类性能;最后,在类向量合并阶段提出基于混合粒子群算法的负载均衡策略(load balancing strategy based on hybrid particle swarm optimization algorithm,LB-HPSO),优化Spark框架中任务节点的负载分配,降低类向量合并时的等待时长,提高模型的并行化效率。实验表明,PDF-MIMW算法的分类效果更佳,同时在大数据环境下的训练效率更高。

JEWELZ&SPARKS

This month we caught up with Julius Voigtlander and Gregor Brechmann,more commonly known as Jewelz&Sparks,to chat about their January 12 debut Guangzhou performance.The German DJ duo formed in 2011 and have since seen success both in the studio and on the global EDM festival circuit,performing at Ultra Europe and Tomorrowland.

ALL EYES ON CHINA:Transfer Frenzy Sparks Worldwide Interest Ahead of New CSL Season

It seems almost incredible to say,but us the Chinese Super League prepares to kick-off the 2016 season,the full gaze of the global football laniily is upon it.The remarkable spending frenzy that has seen the country s record transfer fee broken four times in the close season has ensured that other countries have been forced to take notice.

基于Spark分布式框架的海量星表数据时序重构方法研究

时序重构是时域天文学中的一个重要数据处理步骤,也是拟合光变曲线、开展时域分析研究的基础。Hadoop、Spark这类MapReduce分布式模型在执行过程中分布式集群节点间的任务比较独立,需要跨节点的数据传输量较少。提出了非阻塞异步执行流程,每个分布式进程完全针对独立天区的数据进行连续处理,而分块边缘的新增天体导致的其他节点的新增证认任务延时批量追加,并且会根据各进程间的进度不同确定追加方式,保证证认计算没有遗漏,从而在提高并发效率的同时保证算法的精度。此外,对两表间的不同Join策略从理论和实验两个角度进行了研究并提出了免Join策略。最后通过基于Spark分布式框架的高效时序重构系统的设计完成了以上研究的验证。实验表明,与以往研究结果相比,该时序重构算法效率提升明显,为时域天文学中的天文时序数据分析的开展打下了良好的基础。