Exploring visitors’visual perception along the spatial sequence in temple heritage spaces by quantitative GIS methods:a case study of the Daming Temple,Yangzhou City,China

The Daming Temple,built during 457–464 C.E.,is one of the developing ancient temple heritage spaces located in Yangzhou city,P.R.China.Over the past 60 years,variation in visitors’spatial perception along the tour routes in the temple has occurred.This research attempts to reveal the changes in visitors’visual perception along the spatial sequences at 3 different times(i.e.,1962,1973 and 2022).A quantitative GIS-based method,which includes analysing the distribution of visitors’spatial preferences and spatial configuration,is proposed.Digital landscape tools and quantitative estimation methods are used,including mapping within Rhinoceros software,the kernel density estimation(KDE)method within ArcGIS software and spatial syntax analysis within DepthMap software.Extracted geodata from 500 photographs of the heritage space taken by volunteer visitors are analysed within the GIS environment.Values of the mean depth(MD)at both levels of visibility and accessibility are calculated within the visibility graph analysis(VGA)model.Comparisons between the visual preferences of the visitors and the spatial configuration along the spatial sequence are conducted.The results indicate that the spatial sequence has a significant impact on visitors’visual preferences and tour routes.The phenomenon of spatial sequence among dynamic temporal variations and the effects of narrative spaces along the spatial sequence are highlighted and explained,which reveal the relationship between visitors’geospatial preference and the spatial configuration of the temple.Some suggestions are put forwards for further studies on the revitalisation and management of East Asian ancient temple heritage spaces.

Spatial distribution features of sequence types of moderate and strong earthquake in Chinese mainland

Based on 294 earthquake sequences with magnitude greater than or equal to 5.0 occurred in Chinese mainland since 1970, the spatial distribution features of sequence types have been studied. In southwestern China, it takes mainshock-aftershock sequence type （MAT） as the major in Chuan-Dian rhombic block and concerned Xianshuihe-Anninghe-Xiaojiang seismic belt, as well as in Jinshajiang-Honghe seismic belt. Multiple mainshock type （MMT） mainly distributes in western Yunnan, and Longlin and Lancang areas in Tengchong-Baoshan block in west of Nujiang-Lancangjiang fault zone. A few isolated earthquake type （IET） mainly occurred in northwestern Sichuan and there is no IET occurred in Yunnan region. In northwestern China, it takes mainshock-aftershock sequence type （MAT） as the major in west segment of South Tianshan in Xinjiang region. Some MMT also occurred in this area in the intersection of Kalpin block and the Puchang fault zone. It takes IET as the major in middle Tianshan in Xinjiang. Along the Qilianshan seismic belt, most of sequences are MAT. In Qinghai region, it takes MAT as the major, but the regional feature of the spatial distribution of sequence types is not very clear. In North China, it takes MAT as the major in Yinshan-Yanshan-Bohai seismic belt, north edge of North China, and in Hebei plain seismic belt, as well as in sub-plate of lower river area of Yangtze River. In intersection of north segment of Shanxi seismic belt and the NW-trending Yinshan-Yanshan-Bohai seismic belt, there are several moderate or strong MMT with magnitude from 5.0 to 6.0 occurred. In south of North China around the latitude line of 35°N, it takes IET as the major. The spatial distribution of sequence types is relevant to the patterns of tectonic movements. MAT is mostly produced by the ruptures of locked units or asperities or the neonatal separating segments inside the fault zones. MMT is generally relevant to the conjugate structures or intersection of many tectonic settings. Further extension of simple fault often produces IET. Spatial distribution of sequence types is also correlative to the regional and deep environment of crustal medium to some extent. MAT mainly distributes in high velocity area in upper crust or in the transition zone between high velocity area and low velocity area, MMT mostly occurred in the low velocity area in upper crust.

Dynamic heterogeneity of colorectal cancer during progression revealed clinical risk-associated cell types and regulations in single-cell resolution and spatial context

Background:Tumor heterogeneity is contributed by tumor cells and the microenvironment.Dynamics of tumor heterogeneity during colorectal cancer(CRC)progression have not been elucidated.Methods:Eight single-cell RNA sequencing(scRNA-seq)data sets of CRC were included.Milo was utilized to reveal the differential abundance of cell clusters during progression.The differentiation trajectory was imputed by using the Palantir algorithm and metabolic states were assessed by using scMetabolism.Three spatial transcription sequencing(ST-seq)data sets of CRC were used to validate cell-type abundances and colocalization.Cancer-associated regulatory hubs were defined as communication networks affecting tumor biological behaviors.Finally,quantitative reverse transcription polymerase chain reaction and immunohistochemistry staining were performed for validation.Results:TM4SF1t,SOX4t,and MKI67t tumor cells;CXCL12t cancer-associated fibroblasts;CD4t resident memory T cells;Treg;IgAt plasma cells;and several myeloid subsets were enriched in stage IV CRC,most of which were associated with overall survival of patients.Trajectory analysis indicated that tumor cells from patients with advanced-stage CRC were less differentiated,when metabolic heterogeneity showed a highest metabolic signature in terminal states of stromal cells,T cells,and myeloid cells.Moreover,ST-seq validated cell-type abundance in a spatial context and also revealed the correlation of immune infiltration between tertiary lymphoid structures and tumors followed by validation in our cohort.Importantly,analysis of cancer-associated regulatory hubs revealed a cascade of activated pathways including leukocyte apoptotic process,MAPK pathway,myeloid leukocyte differentiation,and angiogenesis during CRC progression.Conclusions:Tumor heterogeneity was dynamic during progression,with the enrichment of immunosuppressive Treg,myeloid cells,and fibrotic cells.The differential state of tumor cells was associated with cancer staging.Assessment of cancer-associated regulatory hubs suggested impaired antitumor immunity and increased metastatic ability during CRC progression.

ScRNA-seq and ST-seq in liver research

Spatial transcriptomics,which combine gene expression data with spatial information,has quickly expanded in recent years.With application of this method in liver research,our knowledge about liver development,regeneration,and diseases have been greatly improved.While this field is moving forward,a variety of problems still need to be addressed,including sensitivity,limited capacity to obtain exact single-cell information,data processing methods,as well as others.Methods like single-cell RNA sequencing(scRNA-seq)are usually used together with spatial transcriptome sequencing(ST-seq)to clarify cell-specific gene expression.In this review,we explore how advances of scRNA-seq and ST-seq,especially ST-seq,will pave the way to new opportunities to investigate fundamental questions in liver research.Finally,we will discuss the strengths,limitations,and future perspectives of ST-seq in liver research.