Spatial-resolved metabolomics reveals tissue-specific metabolic reprogramming in diabetic nephropathy by using mass spectrometry imaging

Detailed knowledge on tissue-specific metabolic reprogramming in diabetic nephropathy(DN)is vital for more accurate understanding the molecular pathological signature and developing novel therapeutic strategies.In the present study,a spatial-resolved metabolomics approach based on air flowassisted desorption electrospray ionization(AFADESI)and matrix-assisted laser desorption ionization(MALDI)integrated mass spectrometry imaging(MSI)was proposed to investigate tissue-specific metabolic alterations in the kidneys of high-fat diet-fed and streptozotocin(STZ)-treated DN rats and the therapeutic effect of astragalosideⅣ,a potential anti-diabetic drug,against DN.As a result,a wide range of functional metabolites including sugars,amino acids,nucleotides and their derivatives,fatty acids,phospholipids,sphingolipids,glycerides,carnitine and its derivatives,vitamins,peptides,and metal ions associated with DN were identified and their unique distribution patterns in the rat kidney were visualized with high chemical specificity and high spatial resolution.These region-specific metabolic disturbances were ameliorated by repeated oral administration of astragaloside Ⅳ(100 mg/kg)for 12 weeks.This study provided more comprehensive and detailed information about the tissue-specific metabolic reprogramming and molecular pathological signature in the kidney of diabetic rats.These findings highlighted the promising potential of AFADESI and MALDI integrated MSI based metabolomics approach for application in metabolic kidney diseases.

Characteristics of plasma in a novel laser-assisted pulsed plasma thruster

A novel laser-assisted pulsed plasma thruster(LA-PPT)is proposed as an electric propulsion thruster,which separates laser ablation and electromagnetic acceleration.It aims for a higher specific impulse than that achieved with conventional LA-PPTs.Owing to the short-time discharge and the novel configuration,the physical mechanism of the discharge is unclear.Time and spatial-resolved optical emission spectroscopy was applied to investigate the variation in the plasma properties in the thruster discharge channel.The plasma species,electron temperature,and electron density were obtained and discussed.Our investigation revealed that there were H_(α),H_(β),H_(γ),H_(ε) atoms,CⅠ,CⅡ,CⅢ,CⅣ,ClⅠ,ClⅡparticles,and a small amount of CH,C_(3),C_(2),H_(2) neutral molecular groups in the plasma.The electron temperature of the discharge channel of the thruster was within 0.6–4.9 e V,and the electron density was within(1.1–3.0)×10^(18)cm^(-3),which shows that the optical emission spectroscopy method is to measure the electron excitation temperature and electron density in heavy particles.But the Langmuir probe method is to measure the temperature and density of free electrons.The use of laser instead of spark plug as the ignition mode significantly changed the plasma distribution in the discharge channel.Unlike the conventional PPT,which has high electron density near the thruster surface,LAPPT showed relatively large electron density at the thruster outlet,which increased the thruster specific impulse.In addition,the change in the ignition mode enabled the electron density in the LA-PPT discharge channel to be higher than that in the conventional PPT.This proves that the ignition mode with laser replacing the spark plug effectively optimised the PPT performance.