目的建立自动化磁珠标本制备法(automated magnetic specimen preparation,MSPE)提取联合液相色谱串联质谱(liquid chromatography-tandem mass spectrometry,LC-MS/MS)检测人血清中睾酮(testosterone,T)方法并评估其性能。方法选取2023...目的建立自动化磁珠标本制备法(automated magnetic specimen preparation,MSPE)提取联合液相色谱串联质谱(liquid chromatography-tandem mass spectrometry,LC-MS/MS)检测人血清中睾酮(testosterone,T)方法并评估其性能。方法选取2023年4~6月在重庆市开州区人民医院就诊的150例患者作为研究对象,采用MSPE联合LCMS/MS方法检测患者的血清睾酮,排除患者血清溶血、乳糜、黄疸等异常,评价该方法的特异度、线性、定量限、回收率、精密度及基质效应。同时比较化学发光免疫法(chemiluminescence immunoassay,CLIA)与MSPE联合LC-MS/MS检测两种方法结果的一致性。结果MSPE联合LC-MS/MS方法检测睾酮的检测限为0.005 ng/ml,定量限为0.05 ng/ml,该方法在0.05~15 ng/ml范围内相关性良好,r^(2)>0.99;批内、批间、总精密度均小于15%;回收率为105.5%~106.2%;血清内标与分析物的基质效应接近;与CLIA方法比较,两种方法检测结果相关性较好(r=0.9971),相对百分偏差小于15%。结论MSPE提取联合LC-MS/MS检测人血清中睾酮性能良好,能够满足人血清样品中睾酮的定量分析需求。展开更多
The Jominy end quench machine was designed, manufactured and tested. The manganese steel was developed. The as-cast manganese steels were quenched in water at different austenising temperatures and hardenability test ...The Jominy end quench machine was designed, manufactured and tested. The manganese steel was developed. The as-cast manganese steels were quenched in water at different austenising temperatures and hardenability test was carried out on the samples cut at different distances from the quenched surface with the use of the manufactured Jominy end quench machine. The optical microscope was used to investigate the microstructure of the cut samples. Results revealed that the Jominy end quench machine worked effectively with short cycle time and improved water management system. The extremely rapid cooling prevents the decomposition of the chromium carbides. However, the hardness values of the samples decreased with increase in the distance from the quenched surface and the size of the chromium carbides increased with austentic temperatures which is responsible for reduction in the hardness values of the quenched samples at higher austentinic temperature. The manufactured Jominy end quench machine has advantages of improved water management system and short processing time over the existing ones.展开更多
文摘The Jominy end quench machine was designed, manufactured and tested. The manganese steel was developed. The as-cast manganese steels were quenched in water at different austenising temperatures and hardenability test was carried out on the samples cut at different distances from the quenched surface with the use of the manufactured Jominy end quench machine. The optical microscope was used to investigate the microstructure of the cut samples. Results revealed that the Jominy end quench machine worked effectively with short cycle time and improved water management system. The extremely rapid cooling prevents the decomposition of the chromium carbides. However, the hardness values of the samples decreased with increase in the distance from the quenched surface and the size of the chromium carbides increased with austentic temperatures which is responsible for reduction in the hardness values of the quenched samples at higher austentinic temperature. The manufactured Jominy end quench machine has advantages of improved water management system and short processing time over the existing ones.
