Aim:To investigate the impact of abnormal sperm morphology using the sperm deformity index (SDI) on reactive oxygen species (ROS) production and its correlation with sperm DNA damage.Methods:Semen samples were collect...Aim:To investigate the impact of abnormal sperm morphology using the sperm deformity index (SDI) on reactive oxygen species (ROS) production and its correlation with sperm DNA damage.Methods:Semen samples were collected from men undergoing infertility screening (n=7) and healthy donors (n=6).Mature spermatozoa were isolated and incubated with 5 mmol/L β-nicotinamide adenine dinucleotide phosphate (NADPH) for up to 24 h to induce ROS.Sperm morphology was evaluated using strict Tygerberg's criteria and the SDI.ROS levels and DNA damage were assessed using chemiluminescence and terminal deoxynucleotidyl transferase-mediated fluorescein- dUTP nick end labeling (TUNEL) assays,respectively.Results:SDI values (median [interquartiles]) were higher in patients than donors (2 [1.8,2.1] vs.1.53 [1.52,1.58],P=0.008).Aliquots treated with NADPH showed higher ROS levels (1.22 [0.30,1.87] vs.0.39 [0.10,0.57],P=0.03) and higher incidence of DNA damage than those not treated (10 [4.69,24.85] vs.3.85 [2.58,5.10],P=0.008).Higher DNA damage was also seen following 24 h of incubation in patients compared to donors.SDI correlated with the percentage increase in sperm DNA damage following incubation for 24 h in samples treated with NADPH (r=0.7,P=0.008) and controls (r=0.58,P=0.04). Conclusion:SDI may be a useful tool in identifying potential infertile males with abnormal prevalence of oxidative stress (OS)-induced DNA damage.NADPH plays a role in ROS-mediated sperm DNA damage,which appears to be more evident in infertile patients with semen samples containing a high incidence of morphologically abnormal spermatozoa.展开更多
Reliable molecular biomarkers to predict fertility remain scarce.The current study investigated the potential of testis-specific circBOULE RNAs as biomarkers for male infertility and sperm quality.Using reverse transc...Reliable molecular biomarkers to predict fertility remain scarce.The current study investigated the potential of testis-specific circBOULE RNAs as biomarkers for male infertility and sperm quality.Using reverse transcription-PCR and real-time reverse transcription-PCR assays,we identified seven circular RNAs from the human BOULE gene in human sperm.We observed that the expression level of circEx3-6 was significantly reduced in asthenozoospermia,while the expression levels of both circEx2-6 and circEx2-7 were decreased in terato-zoospermia,compared with the controls.Furthermore,we demonstrated that the expression level of circEx2-6 was negatively correlated with the sperm DNA fragmentation index,and the expression level of circEx2-7 was correlated with both fertilization and cleavage rates in those treated with the assisted reproductive technologies.Further functional analyses in a transgenic fly model supported the roles of circBOULE RNAs in sperm development and human male fertility.Collectively,our findings support that sperm circBOULE RNAs may serve as diagnostic biomarkers for assessing sperm motility and DNA quality.Therefore,clinical application and significance of sperm circBOULE RNAs in the assisted reproductive technologies warrant further investigation.展开更多
目的分析不育男性精子DNA碎片与精液常规参数的关系。方法非随机选取2021年7月—2023年6月罗定市人民医院收治的100例不育男性患者为研究对象,检测其精液样本的精液常规参数和精子DNA碎片指数(Sperm DNA Fragmentation Index,DFI),根据...目的分析不育男性精子DNA碎片与精液常规参数的关系。方法非随机选取2021年7月—2023年6月罗定市人民医院收治的100例不育男性患者为研究对象,检测其精液样本的精液常规参数和精子DNA碎片指数(Sperm DNA Fragmentation Index,DFI),根据DFI水平分为A组(DFI≤10%,25例)、B组(10%<DFI<20%,41例)、C组(DFI≥20%,34例),对比3组的精子浓度、精子活率,分析精子浓度、精子活率与精子DFI的相关性。结果A组患者的精子浓度为(128.56±15.15)×10^(6)/mL,高于B组(75.59±13.98)×10^(6)/mL、C组(42.15±8.48)×10^(6)/mL,差异有统计学意义(F=10.688,P=0.005)。A组患者精子存活率高于其他两组,差异有统计学意义(F=39.286,P<0.001)。精子DFI与精子存活率与浓度呈负相关(r=-0.295、-0.354,P均<0.05)。结论患者DFI数值越高,其精子存活率与浓度越低,应着重关注不育男性患者的DFI指数水平。展开更多
文摘Aim:To investigate the impact of abnormal sperm morphology using the sperm deformity index (SDI) on reactive oxygen species (ROS) production and its correlation with sperm DNA damage.Methods:Semen samples were collected from men undergoing infertility screening (n=7) and healthy donors (n=6).Mature spermatozoa were isolated and incubated with 5 mmol/L β-nicotinamide adenine dinucleotide phosphate (NADPH) for up to 24 h to induce ROS.Sperm morphology was evaluated using strict Tygerberg's criteria and the SDI.ROS levels and DNA damage were assessed using chemiluminescence and terminal deoxynucleotidyl transferase-mediated fluorescein- dUTP nick end labeling (TUNEL) assays,respectively.Results:SDI values (median [interquartiles]) were higher in patients than donors (2 [1.8,2.1] vs.1.53 [1.52,1.58],P=0.008).Aliquots treated with NADPH showed higher ROS levels (1.22 [0.30,1.87] vs.0.39 [0.10,0.57],P=0.03) and higher incidence of DNA damage than those not treated (10 [4.69,24.85] vs.3.85 [2.58,5.10],P=0.008).Higher DNA damage was also seen following 24 h of incubation in patients compared to donors.SDI correlated with the percentage increase in sperm DNA damage following incubation for 24 h in samples treated with NADPH (r=0.7,P=0.008) and controls (r=0.58,P=0.04). Conclusion:SDI may be a useful tool in identifying potential infertile males with abnormal prevalence of oxidative stress (OS)-induced DNA damage.NADPH plays a role in ROS-mediated sperm DNA damage,which appears to be more evident in infertile patients with semen samples containing a high incidence of morphologically abnormal spermatozoa.
基金supported by the National Natural Science Foundation of China(Grant Nos.31970792 and 31771652).
文摘Reliable molecular biomarkers to predict fertility remain scarce.The current study investigated the potential of testis-specific circBOULE RNAs as biomarkers for male infertility and sperm quality.Using reverse transcription-PCR and real-time reverse transcription-PCR assays,we identified seven circular RNAs from the human BOULE gene in human sperm.We observed that the expression level of circEx3-6 was significantly reduced in asthenozoospermia,while the expression levels of both circEx2-6 and circEx2-7 were decreased in terato-zoospermia,compared with the controls.Furthermore,we demonstrated that the expression level of circEx2-6 was negatively correlated with the sperm DNA fragmentation index,and the expression level of circEx2-7 was correlated with both fertilization and cleavage rates in those treated with the assisted reproductive technologies.Further functional analyses in a transgenic fly model supported the roles of circBOULE RNAs in sperm development and human male fertility.Collectively,our findings support that sperm circBOULE RNAs may serve as diagnostic biomarkers for assessing sperm motility and DNA quality.Therefore,clinical application and significance of sperm circBOULE RNAs in the assisted reproductive technologies warrant further investigation.
文摘目的分析不育男性精子DNA碎片与精液常规参数的关系。方法非随机选取2021年7月—2023年6月罗定市人民医院收治的100例不育男性患者为研究对象,检测其精液样本的精液常规参数和精子DNA碎片指数(Sperm DNA Fragmentation Index,DFI),根据DFI水平分为A组(DFI≤10%,25例)、B组(10%<DFI<20%,41例)、C组(DFI≥20%,34例),对比3组的精子浓度、精子活率,分析精子浓度、精子活率与精子DFI的相关性。结果A组患者的精子浓度为(128.56±15.15)×10^(6)/mL,高于B组(75.59±13.98)×10^(6)/mL、C组(42.15±8.48)×10^(6)/mL,差异有统计学意义(F=10.688,P=0.005)。A组患者精子存活率高于其他两组,差异有统计学意义(F=39.286,P<0.001)。精子DFI与精子存活率与浓度呈负相关(r=-0.295、-0.354,P均<0.05)。结论患者DFI数值越高,其精子存活率与浓度越低,应着重关注不育男性患者的DFI指数水平。
