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Observation on Sperm Penetration Rate of Infertile Men with Varicocele at 1.5 to 2 Years after Varicolectomy
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作者 梁蔚波 朱伟杰 苏泽轩 《Journal of Reproduction and Contraception》 CAS 2002年第1期10-15,共6页
Objective To observe the sperm penetration rate among treated but 'unsuccessful' infertile men with varicocele Material & Methods Twenty nine infertile males with II°(n=17) or III°(n=12) var... Objective To observe the sperm penetration rate among treated but 'unsuccessful' infertile men with varicocele Material & Methods Twenty nine infertile males with II°(n=17) or III°(n=12) varicocele were enrolled into this study. They underwent a varicolectomy, but their spouses had not achieved a pregnancy after 1.5~2 years of treatment. The sperm zona-free hamster oocyte penetration assay (SPA) was used to compare the sperm penetration rate (PR) before treatment and 1.5~2 years after treatment. Results After varicolectomy, enhanced PR were observed in 7 cases of the II°group and in 1 case of the III°group. However, the PR values of these 8 cases were still below 20% PR-level. Conclusion Among the treated but 'unsuccessful' men, some patients could obtain improvements on PR after varicolectomy. But PRs of men with III°grade varicele would still remain a low PR-level which indicate varicolectomy is ineffective in increasing PR among part of sever varicocele patients. SPA is a useful test for evaluating the fertilizing potential of treated infertile males with varicocele. 展开更多
关键词 VARICOCELE zona-free hamster oocyte penetration assay(spa) sperm penetration rate
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γ-氨基丁酸诱发人精子顶体反应及其受精能力 被引量:15
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作者 袁玉英 何春娜 石其贤 《生理学报》 CAS CSCD 北大核心 1998年第3期326-332,共7页
本文的目的是为了探讨γ-氨基丁酸(GABA)是否可激发人精子顶体反应(AR)。受精能力及其可能的作用方式。实验采用金霉素荧光染色法(CFC)、胞内游离Ca2+测定和精子穿透去透明带仓鼠卵(SPA)试验,分别评价GABA诱发人精子AR及其穿... 本文的目的是为了探讨γ-氨基丁酸(GABA)是否可激发人精子顶体反应(AR)。受精能力及其可能的作用方式。实验采用金霉素荧光染色法(CFC)、胞内游离Ca2+测定和精子穿透去透明带仓鼠卵(SPA)试验,分别评价GABA诱发人精子AR及其穿卵能力。结果表明:CABA可诱发人获能精子发生AR,且随精子获能进程而显著增加,并存在明显的量效关系,该作用可被P4加强。GABA还可明显地激发胞内钙[Ca2+]i升高。然而,GABA诱发精子AR不仅可被Ca2+螫合剂EGTA所桔抗,而且为Ca2+通道阻滞剂La3+所阻断。此外,GABA还可明显地促进人精子的穿卵能力。据此推论,GABA通过Ca2+介导,调节人精子AN,促进受精率提高。上述结果为激活精子表而GABAA受体及其引起信息传递的研究奠定了基础。 展开更多
关键词 GABA 精子 顶体反应 精子穿卵试验 受精能力
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精索静脉曲张不育患者术后未育者精子穿卵率的远期观察 被引量:1
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作者 梁蔚波 朱伟杰 苏泽轩 《生殖与避孕》 CAS CSCD 北大核心 2002年第3期149-151,共3页
目的 :对精索静脉曲张不育患者术后未育者的精子穿卵率作远期观察。方法 :1双侧经髂窝或经腹腔镜精索静脉高位结扎术 ;2人精子去透明带地鼠卵穿透试验 (SPA)。结果 :2 9例精索静脉曲张不育患者 ,术后 1 .5~ 2年随访仍未育 ,作 SPA,与术... 目的 :对精索静脉曲张不育患者术后未育者的精子穿卵率作远期观察。方法 :1双侧经髂窝或经腹腔镜精索静脉高位结扎术 ;2人精子去透明带地鼠卵穿透试验 (SPA)。结果 :2 9例精索静脉曲张不育患者 ,术后 1 .5~ 2年随访仍未育 ,作 SPA,与术前 SPA穿卵率对比 ,精索静脉曲张 °组 (n=1 7)有 7例改善了穿卵率 ,精索静脉曲张 °组 (n=1 2 )有 2例改善了穿卵率。但两组穿卵率的升高幅度不大。结论 :精索静脉曲张不育患者术后较长时间仍未育者 ,虽部份患者的精子穿卵率已有改善 ,但穿卵率的增加程度不高。术前精索静脉曲张严重的患者 ,术后可能难以提高穿卵率。应建立 SPA对患者术后精子受精能力作评估 ,以及时调整治疗措施。 展开更多
关键词 精索静脉曲张症 去透明带地鼠卵穿透试验 spa 精子 穿卵率
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The hamster egg penetration test may decrease intracytoplasmic sperm injection utilization while maintaining high conventional fertilization rates 被引量:1
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作者 Yetunde Ibrahim Brett Einerson +2 位作者 Douglas T Carrell Benjamin R Emery Erica Johnstone 《Asian Journal of Andrology》 SCIE CAS CSCD 2021年第1期11-15,共5页
This was a cohort study of in vitro fertilization(IVF)subjects at the University of Utah,Salt Lake City(UT,USA)utilizing partner sperm.Cycles where both the hamster egg penetration test(HEPT)and semen analysis were pe... This was a cohort study of in vitro fertilization(IVF)subjects at the University of Utah,Salt Lake City(UT,USA)utilizing partner sperm.Cycles where both the hamster egg penetration test(HEPT)and semen analysis were performed within 2 years prior to IVF cycles were stratified into four groups based on a normal or an abnormal HEPT and morphology.The mean conventional and intracytoplasmic sperm injection(ICSI)fertilization rates were calculated in each group.We performed a univariate analysis on the primary outcome comparing clinically interesting subjects.We performed a cost-effectiveness analysis of a policy of HEPT versus universal ICSI in couples with an abnormal morphology.Among patients with a normal HEPT,there was no difference in the mean conventional fertilization rates between those with a normal and an abnormal morphology.There was no difference in the mean conventional fertilization rates between subjects with a normal morphology without a hamster test and those with a normal HEPT without a morphology assessment.In 1000 simulated cycles with an abnormal morphology,a policy of HEPT was cost saving compared to universal ICSI,yet produced similar fertilization rates.The HEPT is similar to the World Health Organization edition 5(WHO-5)morphology in predicting successful conventional fertilization while allowing decreased utilization of ICSI.A policy of HEPT for males with abnormal morphology saves cost in selecting couples for a fertilization method. 展开更多
关键词 conventional fertilization hamster egg penetration test intracytoplasmic sperm injection semen analysis sperm penetration assay
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