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Effects of Murine Cytomegalovirus Infection on Sperm Viability in Mice
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作者 熊锦文 熊承良 +2 位作者 田永红 胡廉 卫海 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 2006年第1期130-132,共3页
In order to explore the effects of testicular infection of murine cytomegalovirus (MCMV) on mature sperm viability at different periods following MCMV inoculation in mice, 91 BALB/c mice without MCMV infection were ... In order to explore the effects of testicular infection of murine cytomegalovirus (MCMV) on mature sperm viability at different periods following MCMV inoculation in mice, 91 BALB/c mice without MCMV infection were randomly divided into two groups: an experimental group (n= 56) and a control group (n=35). The mice in the experimental group were treated by inoculating MCMV intratesticularly, while those in the controlled group were directly inoculated with DMEM without MCMV. The mice in both groups were sacrificed separately on the day 1,1.5, 2, 4, 6, 9 and 14 post-inoculation (D1, 1.5, 2, 4, 6, 9 and 14 PI). The MCMV M83 mRNA gene was detected in the testis by in situ hybridization (ISH) with MCMV late-mRNA probe labeled with digoxin. Sperm viability of mature sperm in the epididymis cauda was measured. The results demonstrated the positive signal of ISH of MCMV was found mainly in the cytoplasm of the testicular interstitial cells and spermatogenic cells in the experimental group. Compared with that in the controlled group, the sperm viability in the experimental group was decreased significantly on D1 PI and D1.5 PI (P〈 0.05). No statistically significant difference in the sperm viability was found after D2 PI between two groups (P〉0.05). This suggested that sperm viability in mice might be descended significantly shortly after MCMV infection and might return to normal with time, indicating that MCMV acute infection might temporarily degrade sperm quality and influence procreation transiently. 展开更多
关键词 murine cytomegalovirus TESTIS in situ hybridization sperm viability
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Sperm quality improvement after date seed oil in Vitro supplementation in spontaneous and induced oxidative stress 被引量:4
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作者 Ben A. Fatma Chakroun F. Nozha +3 位作者 Dammak Ines Attia Hamadi Hentati Basma Ammar K. Leila 《Asian Journal of Andrology》 SCIE CAS CSCD 2009年第3期393-398,共6页
In vitro supplementation with date seed oil (DSO) can protect spermatozoa against hydrogen peroxide (HiO2)- mediated damage and can improve sperm function, possibly owing to antioxidant properties. We tested the a... In vitro supplementation with date seed oil (DSO) can protect spermatozoa against hydrogen peroxide (HiO2)- mediated damage and can improve sperm function, possibly owing to antioxidant properties. We tested the antioxidant effects of DSO on human sperm motility, sperm viability, reacted acrosome and lipid peroxidation assessed in vitro after H202-mediated oxidative damage in spermatozoa. Sixteen patients (mean age: 35 years; range: 25-45 years) referred to the Histology-Embryology Laboratory of the Medicine Faculty of Sfax for semen analysis after 12-24 months of sexual intercourse without conception were selected. After spermiogram, sperm selection by twointerface discontinuous Sill Select gradient was performed, and selected spermatozoa were used in four experimental assays: control; incubation with 100um H2O2; incubation with 0.1% DSO; and co-incubation with 0.1% DSO and 100 um H2O2. Motility and viability were determined using World Health Organization criteria. Acrosome reaction and lipid peroxidation were assessed by staining with fluorescein isothiocyanate-Pisum sativum and spectrophotometric measurement of malondialdehyde, respectively. Results showed that incubation with H2O2 alone led to a significant increase in lipid peroxidation (57.83%, P 〈 0.05) associated with a significant decrease in sperm motility, sperm viability (after 30 min and 24 h) and percentage of reacted acrosome (P 〈 0.05). Date seed oil im- proved sperm motility after 24 h of incubation (P 〈 0.05) and protected spermatozoa against the deleterious effects of H2O2 on motility, viability, acrosome reaction and lipid peroxidation. We conclude that supplementation with DSO may have a function in antioxidant protection against male infertility. 展开更多
关键词 acrosome reaction date seed oil in vitro lipid peroxidation sperm motility sperm viability
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Sperm cryodamage occurs after rapid freezing phase:flow cytometry approach and antioxidant enzymes activity at different stages of cryopreservation 被引量:6
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作者 L.S.Castro T.R.S.Hamilton +4 位作者 C.M.Mendes M.Nichi V.H.Barnabe J.A.Visintin M.E.O.A.Assumpcao 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2016年第4期495-503,共9页
Background: In order to improve the efficiency of bovine sperm cryopreservation process, it is important to understand how spermatozoa respond to differences in temperature as well as the ability to recover its own m... Background: In order to improve the efficiency of bovine sperm cryopreservation process, it is important to understand how spermatozoa respond to differences in temperature as well as the ability to recover its own metabolism. The combination between flow cytometry approach and antioxidant enzymes activity allows a more sensible evaluation of sperm cell during cryopreservation. The aim of this study was to evaluate sperm attributes and antioxidant enzymes activity during different stages of cryopreservation process. Semen samples from Holstein bulls (n = 4) were separated in 3 treatments: fresh (37 ℃); cooled (5 ℃); and thawed. Evaluation occurred at 0 h and 2 h after incubation. Membrane integrity, mitochondrial membrane potential (MMP) and DNA damages were evaluated by flow cytometry; activities of antioxidant enzymes such as catalase, superoxide dismutase and gluthatione peroxidase were measured by spectrofotometry. Results: There was an increase in the percentage of sperm with DNA damage in the thawed group, compared to fresh and cooled, and for 2 hs of incubation when compared to 0 h. Considering MMP, there was an increase in the percentage of cells with medium potential in thawed group when compared to fresh and cooled groups. Opposingly, a decrease was observed in the thawed group considering high mitochondrial potential. Also in the thawed group, there was an increase on cells with damaged acrosome and membrane when compared to fresh and cooled groups. Significant correlations were found between antioxidant enzymes activity and membrane or mitochondrial parameters. Conclusion: Based on our results, we conclude that cryopreservation affects cellular and DNA integrity and that the critical moment is when sperm cells are exposed to freezing temperature. Also, our study indicates that intracellular antioxidant machinery (SOD and GPX enzymes) is not enough to control cryodamage. 展开更多
关键词 BOVINE DNA integrity JC-1 sperm viability
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Predictive value of hypo-osmotic swelling test to identifyviable non-motile sperm 被引量:3
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作者 William M.Buckett 《Asian Journal of Andrology》 SCIE CAS CSCD 2003年第3期209-212,共4页
Aim: To determine the predictive value of the hypo-osmotic swelling (HOS) test to identify viable, non-motile sperm. Methods: Semen samples from 20 men with severe asthenozoospermia underwent traditional seminal analy... Aim: To determine the predictive value of the hypo-osmotic swelling (HOS) test to identify viable, non-motile sperm. Methods: Semen samples from 20 men with severe asthenozoospermia underwent traditional seminal analysis, eosin-nigrosin (EN) staining and the HOS test. A further EN stain was then performed on a HOS pre-treated aliquot and a total of 2000 further sperm examined. Results: The median sperm density was 5.1 million/mL (IQR 4.3-13.1) and the median motility was 3.0 % (IQR 0-7). Seven samples showed complete asthenozoospermia. Initial EN staining showed 59 % viability (range 48-69) despite the poor standard parameters and 47 % (range 33-61) in the complete asthenozoospermia subgroup. The HOS test showed 49.9 % reacted overall (range 40-59) and 41.7 % (range 22-61) in the complete asthenozoospermia subgroup. The combined HOS/EN stain showed the positive predictive value of the HOS test to identify viable sperm was 84.2 % overall and 79.7 % in the complete asthenozoospermia subgroup. Conclusion: The HOS test can effectively predict sperm viability in patients with severe and complete asthenozoospermia. 展开更多
关键词 azoospermia sperm spermmotility hypo-osmotic swelling (HOS) test sperm viability predictive value
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Acute toxicity effects of perfluorooctane sulfonate on sperm vitality,kinematics and fertilization success in zebrafish 被引量:2
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作者 夏继刚 牛翠娟 《Chinese Journal of Oceanology and Limnology》 SCIE CAS CSCD 2017年第4期723-728,共6页
Perfluorooctane sulfonate (PFOS) has emerged as one of the most concerning contaminants in recent years. This study aimed to investigate the acute toxicity effect of PFOS on sperm viability, kinematics and fertiliza... Perfluorooctane sulfonate (PFOS) has emerged as one of the most concerning contaminants in recent years. This study aimed to investigate the acute toxicity effect of PFOS on sperm viability, kinematics and fertilization success in zebrafish (Danio rerio). Sperm were activated in aqueous media containing a range of PFOS concentrations (0, 0.09, 0.9 and 9 mg/L). Viabilities and kinematics of the sperm exposed to different PFOS treatments were assessed via computer-assisted sperm analysis (CASA) at 20, 40, 60, and 80 s after activation. PFOS exposure decreased the percentage of motile sperm, the curvilinear velocity (VCL), and the mean angular displacement (MAD) of spermatozoa, but showed no influence on the straight- line velocity (VSL) or the angular path velocity (VAP). Furthermore, a significant decrease in fertilization success was observed in spermatozoa that were exposed to 0.9 mg/L PFOS or more. These findings indicate that PFOS pollution in natural aquatic environment may be a potential threaten to successful reproduction of fish. 展开更多
关键词 perfluorooctane sulfonate (PFOS) acute toxicity sperm viability sperm kinematics fertilization Success
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Sperm traits differ between winged and wingless males of the ant Cardiocondyla obscurior
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作者 Alexandra SCHREMPF Astrid MOSER +1 位作者 Jacques DELABIE Jürgen HEINZE 《Integrative Zoology》 SCIE CSCD 2016年第6期427-432,共6页
Size and shape of sperm cells vary tremendously throughout the animal kingdom.The adaptive significance of this variation is not fully understood.In addition to sperm-female interactions and the environmental condi... Size and shape of sperm cells vary tremendously throughout the animal kingdom.The adaptive significance of this variation is not fully understood.In addition to sperm-female interactions and the environmental condi­tions,the risk of sperm competition might affect number,morphology and other“quality”traits of sperm.In the male-diphenic ant Cardiocondyla obscurior,winged sneaker males have limited sperm number,because their testes degenerate shortly after adult emergence,as is typical for males of social Hymenoptera.In contrast,wing­less fighter males continuously replenish their sperm supply due to their exceptional lifelong spermatogene­sis.While winged males usually have to compete with several other winged males for virgin queens,wingless males are able to monopolize queens by killing all other rivals.Hence,this presents a unique system to inves­tigate how alternative reproductive tactics and associated physiology affect sperm morphology and viability.We found that sperm-limited males invest into sperm number instead of sperm size.Variance in sperm length is smaller in winged males,probably reflecting that they have to compete with several other males.Finally,sperm viability is equally high in both male phenotypes. 展开更多
关键词 alternative reproductive tactic social insect sperm length sperm viability
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