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Dynamic transcriptome profiles and novel markers in bovine spermatogenesis revealed by single-cell sequencing
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作者 Yuan Gao Fuxia Bai +4 位作者 Qi Zhang Xiaoya An Zhaofei Wang Chuzhao Lei Ruihua Dang 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2024年第7期2362-2378,共17页
Testicular development is an important biological process in male and requires interaction between the male germ cells and somatic cells.However,the mechanisms of testicular development in livestock,particularly in ca... Testicular development is an important biological process in male and requires interaction between the male germ cells and somatic cells.However,the mechanisms of testicular development in livestock,particularly in cattle,are poorly understood.Furthermore,cellular heterogeneity hinders the profiling of different cell types at different developmental stages.In this study,we first performed a single-cell transcriptomic study of the bovine testis development during puberty by using 10×genomics single-cell RNA sequencing(scRNA-seq).By collecting the scRNA-seq data from 11,083 cells from prepubertal and pubertal bovine testes,a high-resolution scRNA-seq atlas was described,identifying 9 somatic and 13 spermatogenic clusters.We also distinguished several stage-specific marker genes for bovine germ cells and somatic cells,such as GRAF2 and MORC1 for SSC(spermatogonial stem cells),HJURP and TCF19 for differentiating spermatogonia,ARSE for immature Sertoli,CLEC12B for mature Sertoli,LOC112441470 for Leydig.In conclusion,we have examined the transcription levels and constructed the single-cell developmental maps of germ cells and somatic cells during testicular development in Angus cattle.The datasets provided new insights into spermatogenesis and testicular somatic cell development in cattle. 展开更多
关键词 scRNA-seq CATTLE spermatogenesis testis development
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Dietary exposure to sulfamethazine,nanoplastics and their binary mixture disrupts the spermatogenesis of marine medaka(Oryzias melastigma)
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作者 Yuting Zhang Ruanni Chen +6 位作者 Zhiqiang Chen Xiaoyu Fu Ziyi Wu Jinwan Chen Lingtian Xie Humin Zong Jingli Mu 《Acta Oceanologica Sinica》 SCIE CAS CSCD 2024年第8期104-110,共7页
In the coastal environment,the co-occurrence of antibiotic and nanoplastic pollution is common.Investigating their individual and combined toxicity to marine organisms is of great necessity.In the present study,the re... In the coastal environment,the co-occurrence of antibiotic and nanoplastic pollution is common.Investigating their individual and combined toxicity to marine organisms is of great necessity.In the present study,the reproductive toxicity of sulfamethazine(SMZ)and nanoplastics(polystyrene,PS)via the dietary route on the spermatogenesis of marine medaka(Oryzias melastigma)was examined.After 30 d of dietary exposure,SMZ alone decreased the gonadosomatic index(GSI)value(~35%)and the proportion of undifferentiated type A spermatogonia(A_(und))(~40%),probably by disrupting the testicular sex hormone production,the spermatogenesis-related growth factor network and the balance of apoptosis.Individual exposure to PS did not affect the GSI value or the proportions of germ cells at different developmental stages,but dysregulated the expression of several spermatogenesis-related genes.Interestingly,the presence of PS alleviated the decreased GSI value caused by SMZ.This alleviation effect was achieved by enhancing the spermatogonia differentiation instead of reversing the suppressed self-renewal of A_(und),suggesting that the mixture of PS and SMZ could cause reproductive effects in a different way.These findings expand our knowledge of threats of ubiquitous antibiotic and nanoplastic pollution to fish reproduction and population. 展开更多
关键词 nanoplastics ANTIBIOTICS spermatogenesis combined toxicity Oryzias melastigma
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Applications of single-cell RNA sequencing in spermatogenesis and molecular evolution
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作者 Wen-Bo Chen Meng-Fei Zhang +1 位作者 Fan Yang Jin-Lian Hua 《Zoological Research》 SCIE CSCD 2024年第3期575-585,共11页
Spermatogenic cell heterogeneity is determined by the complex process of spermatogenesis differentiation.However,effectively revealing the regulatory mechanisms underlying mammalian spermatogenic cell development and ... Spermatogenic cell heterogeneity is determined by the complex process of spermatogenesis differentiation.However,effectively revealing the regulatory mechanisms underlying mammalian spermatogenic cell development and differentiation via traditional methods is difficult.Advances in technology have led to the emergence of many single-cell transcriptome sequencing protocols,which have partially addressed these challenges.In this review,we detail the principles of 10x Genomics technology and summarize the methods for downstream analysis of single-cell transcriptome sequencing data.Furthermore,we explore the role of single-cell transcriptome sequencing in revealing the heterogeneity of testicular ecological niche cells,delineating the establishment and disruption of testicular immune homeostasis during human spermatogenesis,investigating abnormal spermatogenesis in humans,and,ultimately,elucidating the molecular evolution of mammalian spermatogenesis. 展开更多
关键词 Single-cell RNA sequencing(scRNA-seq) spermatogenesis Molecular evolution Sertoli cell
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Assessment of spermatogenesis in camels via seminiferous tubule staging and testosterone profiling
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作者 Saif Ullah Abdul Quddus +7 位作者 Quanwei Wei Wael Ennab Sheeraz Mustafa Jiahao Sun Rahmani Mohammad Malyar Abdul Baseer Dagan Mao Fangxiong Shi 《Asian pacific Journal of Reproduction》 CAS 2024年第3期133-142,共10页
Objective:To investigate the successive morphological stages of spermatogenesis,hormonal regulation,and testosterone profile in dromedary camel reproduction.Methods:Testicular tissue samples were obtained from 12 drom... Objective:To investigate the successive morphological stages of spermatogenesis,hormonal regulation,and testosterone profile in dromedary camel reproduction.Methods:Testicular tissue samples were obtained from 12 dromedary bull camels aged 7 to 8 at a local abattoir.The histological assessment involved tissue processing,hematoxylin and eosin(H&E)staining,and examination under a microscope.Stereological analysis,germ cell identification,and assessment of seminiferous tubules and maturation were conducted.Testosterone assay was performed by radioimmunoassay using blood samples collected at regular intervals.Results:The study revealed 12 phases of the dromedary camel's seminiferous epithelium cycle,highlighting distinct morphological characteristics and cellular processes.Acrosomal migration,maturation,cap formation,and the Golgi-mediated synthesis of proacrosomal vesicles were also explained in dimension,as were the steps of acrosome biogenesis.Spermatids and mature sperm cells were present when spermatogenesis phases were examined.An analysis of the dimensions of seminiferous tubules revealed specific measures for diameter,area,and epithelial height about luminal characteristics.Moreover,there were noticeable variations in the serum testosterone concentrations during the study period,indicating temporal dynamics.Conclusions:This study outlines the spermatogenesis process in dromedary camels across 12 stages,emphasizing cellular dynamics and acrosomal biogenesis.It also provides seminiferous tubule measurements and observes seasonal testosterone fluctuations,offering insights into reproductive regulation and potential strategies for camel breeding conservation. 展开更多
关键词 spermatogenesis CAMEL Testosterone profile Morphological staging
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Pulsatile gonadotropin-releasing hormone therapy induces spermatogenesis in pituitary stalk interruption syndrome:A case report and review of the literature
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作者 Jin-Long Xie Hai-Ying Zhu +5 位作者 Yang Dong Ping-Ping Sun Dan-Dan Qi Su-Xian Luan Yan Zhang Hua-Gang Ma 《World Journal of Clinical Cases》 SCIE 2024年第20期4348-4356,共9页
BACKGROUND Pituitary stalk interruption syndrome(PSIS)is a rare anatomical defect of the pituitary gland falling under the spectrum of holoprosencephaly phenotypes.It is characterized by a deficiency in anterior pitui... BACKGROUND Pituitary stalk interruption syndrome(PSIS)is a rare anatomical defect of the pituitary gland falling under the spectrum of holoprosencephaly phenotypes.It is characterized by a deficiency in anterior pituitary hormones,such as growth hormone,gonadotropins,and thyroid hormones.Due to the syndrome's rarity and nonspecific manifestations,there is a lack of standardized treatment strategies.Consequently,early diagnosis through imaging and on-time intervention are crucial for improving patients’outcomes.CASE SUMMARY A 30-year-old man presented with absent secondary sexual characteristics and azoospermia.Laboratory evaluation revealed a deficiency in gonadotropins,while thyroid function was mostly within normal ranges.Magnetic resonance imaging of the pituitary gland showed pituitary stalk agenesis,hypoplasia of the anterior pituitary,and ectopic posterior pituitary,leading to the diagnosis of PSIS.Initially,the patient underwent 6 mo of gonadotropin therapy without significant changes in hormone levels and secondary sexual characteristics.Pulsatile gonadotropin-releasing hormone therapy was then administered,resulting in the detection of sperm in the semen analysis within 3 mo.After 6 mo,routine semen tests showed normal semen quality.The couple faced challenges in conceiving due to abstinence and underwent three cycles of artificial insemination,which was unsuccessful.They also attempted in vitro fertilization,but unfortunately,the woman experienced a miscarriage 10 wk after the embryo transfer.CONCLUSION Early detection,accurate diagnosis,and timely treatment are crucial in improving the quality of life and fertility of PSIS patients. 展开更多
关键词 Pituitary stalk interruption syndrome Hypogonadotropic hypogonadism spermatogenesis Pulsatile gonadotropinreleasing hormone therapy Case report
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Testis Development and Ultrastructural Features During Spermatogenesis in Cultured Small Yellow Croaker,Larimichthys polyactis
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作者 ZHENG Xuebin WANG Jingqian +6 位作者 GAO Xinming DU Chen HOU Congcong XIE Qingping LIU Feng ZHU Junquan LOU Bao 《Journal of Ocean University of China》 SCIE CAS CSCD 2023年第5期1412-1426,共15页
The small yellow croaker Larimichthys polyactis is an economically important marine fish in Northeast Asia.Currently,its natural resources are threatened by overfishing and environmental pollution.Therefore,research o... The small yellow croaker Larimichthys polyactis is an economically important marine fish in Northeast Asia.Currently,its natural resources are threatened by overfishing and environmental pollution.Therefore,research on the reproductive system of the fish is crucial.Here,we studied the testis development and ultrastructural features of spermatogenesis in cultured L.polyactis using anatomical,histological,and ultrastructural techniques.A pair of testes,consisting of a central sperm duct and radial seminiferous lobules,were observed.The reproduction cycle of testes can be divided into stages I–VI.March to May was confirmed as the breeding season for male L.polyactis,while April is the ideal period for artificial breeding.The male L.polyactis can attain sexual maturity within 1 year.The spermatogenesis of L.polyactis comprised spermatogonium,spermatocyte,spermatid,and mature spermatozoon.The morphology of spermatogenic cells changed obviously during spermiogenesis,including nuclear shaping,midpiece and flagellum formation.The mature sperms consist of an ellipsoidal head,a short midpiece,and a long flagellum.The anterior of the head with a kidney-shaped nucleus can be distinguished.The midpiece is located posterior to the head and includes four to six spherical mitochondria.The flagellum has irregular lateral fins.The testis of L.polyactis is an unrestricted lobular type,with cystic spermatogenesis,type II spermiogenesis,and type II spermatozoa.These features are highly similar to those of other Sciaenid species.Our findings provide useful insights into the mechanism underlying testis development and spermatogenesis of L.polyactis,which can facilitate the artificial breeding of this species. 展开更多
关键词 testis development spermatogenesis Larimichthys polyactis HISTOLOGY ULTRASTRUCTURE
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The effects of hormone-mediated PI3K/AKT signaling on spermatogenesis in Sertoli cells
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作者 YANSHUANG ZHAO WANXI YANG 《BIOCELL》 SCIE 2023年第8期1709-1725,共17页
The phosphoinositide-3-kinase/Akt(PI3K/AKT)signaling pathway is crucial for Sertoli cell development and completing spermatogenesis.Its main role is to promote proliferation and inhibit apoptosis.Many factors activate... The phosphoinositide-3-kinase/Akt(PI3K/AKT)signaling pathway is crucial for Sertoli cell development and completing spermatogenesis.Its main role is to promote proliferation and inhibit apoptosis.Many factors activate the PI3K/AKT pathway,like hormones,such as follicle stimulating hormone(FSH),androgen,estrogen,insulin to name a few.Many of these factors have receptors inside or on the surface of Sertoli cells(SCs).This review summarizes how these hormones directly regulate the PI3K/AKT signaling pathway in SCs,which in turn affects SC proliferation and differentiation.Further,hormone-mediated PI3K/AKT signaling also stimulates SC secretion,which is essential for germ cell development,suggesting an indirect role of PI3K/AKT signaling during spermatogenesis.These functions include promoting spermatogonia proliferation and differentiation,meiosis of spermatocytes,sperm maturation,and their release.This review also provides potential hints for clinically treating male infertility issues like cryptorchidism and Sertoli cell-only syndrome. 展开更多
关键词 HORMONE PI3K/AKT signaling spermatogenesis Sertoli cell secretion Male infertility
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Male Reproductive System and Spermatogenesis in Homoptera(Insecta:Hemiptera) 被引量:7
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作者 田润刚 袁锋 张雅林 《Entomotaxonomia》 CSCD 北大核心 2006年第4期241-253,共13页
Morphology of the male reproductive system, chromosome behaviors during meiosis and spem tail structures in Homoptera and Heteroptera are compared in this paper. The sheathed testis is found in Fulgoroidea and Heterop... Morphology of the male reproductive system, chromosome behaviors during meiosis and spem tail structures in Homoptera and Heteroptera are compared in this paper. The sheathed testis is found in Fulgoroidea and Heteroptera, and unsheathed testis occurs in Cicadoidea, Cicadelloidea, Cercopoidea, Membracoidea, Psyloidea, Aphidoidea, Aleyrodoidea and Coccoidea. The testis also can be divide into three types by the shape of testicular follicles. The sphere-shaped type is found in Cicadoidea, Cicadelloidea, Cercopoidea, Membracoidea, Aphidoidea and Aleyrodoidea, the tube-shaped type observed in Fulgoroidea, Psyloidea and Coccoidea, and the lamella-shaped type represented by Heteroptera. It is suggested the unsheathed testis may be the primitive type in Homoptera. Meiosis can be divided into 6 type at least, i.e. 1) Cicadoid type; 2) Fulgoroid type; 3) Psyloid type; 4) Aphidoid type; 5) Aleyrodoid type; and 6) Coccoid type. At least four groups exhibit a diffuse stage during meiosis prophase l, they are Psyloidea, Fulgoroidea, Coccoidea and Heteroptera. Sperm tail structures are similar to those reported from other insects with a typical 9+9+2 axoneme except that in Aleyrodoidea and Coccoidea whose sperm tail is degenerated. 展开更多
关键词 HEMIPTERA HOMOPTERA reproductive system spermatogenesis MEIOSIS sperm structure
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Effect of sodium arsenite on spermatogenesis, plasma gonadotrophins and testosterone in rats 被引量:25
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作者 Mahitosh Sarkar Gargi Ray Chaudhuri +1 位作者 Aloke Chattopadhyay Narendra Mohan Biswas 《Asian Journal of Andrology》 SCIE CAS CSCD 2003年第1期27-31,共5页
To investigate the effect of arsenic on spermatogenesis. Methods: Mature (4 months old) Wistar rats were intraperitoneally administered sodium arsenite at doses of 4, 5 or 6 mg-kg^-day1 for 26 days. Different varietie... To investigate the effect of arsenic on spermatogenesis. Methods: Mature (4 months old) Wistar rats were intraperitoneally administered sodium arsenite at doses of 4, 5 or 6 mg-kg^-day1 for 26 days. Different varieties of germ cells at stage VII seminiferous epithelium cycle, namely, type A spermatogonia (ASg), preleptotene spermatocytes (pLSc), midpachytene spermatocytes (mPSc) and step 7 spermatids (7Sd) were quantitatively evaluated, along with radioimmunoassay of plasma follicle-stimulating hormone (FSH), lutuneizing hormone (LH), testosterone and assessment of the epididymal sperm count. Results: In the 5 and 6 mg/kg groups, there were significant dose-dependent decreases in the accessory sex organ weights, epididymal sperm count and plasma concentrations of LH, FSH and testosterone with massive degeneration of all the germ cells at stage VII. The changes were insignificant in the 4 mg/kg group. Conclusion: Arsenite has a suppressive influence on spermatogenesis and gonadotrophin and testosterone release in rats. 展开更多
关键词 ARSENITE spermatogenesis GONADOTROPHINS TESTOSTERONE TESTIS
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Regulation of spermatogenesis by paracrine/autocrine testicular factors 被引量:22
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作者 Mahmoud Huleihel Eitan Lunenfeld 《Asian Journal of Andrology》 SCIE CAS CSCD 2004年第3期259-268,共10页
Spermatogenesis is a complex process regulated by endocrine and testicular paracrine/autocrine factors. Gonadotropins are involved in the regulation of several testicular paracrine factors, mainly of the IL-1 family a... Spermatogenesis is a complex process regulated by endocrine and testicular paracrine/autocrine factors. Gonadotropins are involved in the regulation of several testicular paracrine factors, mainly of the IL-1 family and testicular hormones. Testicular cytokines and growth factors (such as IL-1, IL-6, TNF, IFN-γ, LIF and SCF) were shown to affect both the germ cell proliferation and the Leydig and Sertoli cells functions and secretion. Cytokines and growth factors are produced by immune cells and in the interstitial and seminiferous tubular compartments by various testicular cells, including Sertoli, Leydig, peritubular cells, spermatogonia, differentiated spermatogonia and even spermatozoa. Corresponding cytokine and growth factor receptors were demonstrated on some of the testicular cells. These cytokines also control the secretion of the gonadotropins and testosterone in the testis. Under pathological conditions the levels of pro-inflammatory cytokines are increased and negatively affected spermatogenesis. Thus, the expression levels and the mechanisms involved in the regulation of testicular paracrine/autocrine factors should be considered in future therapeutic strategies for male infertility. 展开更多
关键词 spermatogenesis PARACRINE AUTOCRINE CYTOKINE growth factor
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Effect of Lepidium meyenii(maca)roots on spermatogenesis of male rats 被引量:22
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作者 Gustavo F.Gonzales Ana Ruiz +2 位作者 Carla Gonzales León Villegas Amanda Cordova 《Asian Journal of Andrology》 SCIE CAS CSCD 2001年第3期231-233,共3页
Aim:To determine the effect of oral administration of an aqueous extract from the roots of Lepidium meyenii(maca)on spermatogenesis in adult male rats.Methods;Male rats received an aqueous extract of the root(66.7 mg ... Aim:To determine the effect of oral administration of an aqueous extract from the roots of Lepidium meyenii(maca)on spermatogenesis in adult male rats.Methods;Male rats received an aqueous extract of the root(66.7 mg in onemL)twice a day for 14 consecutive days.Results:Treatment with Lepidium meyenii resulted in an increase in theweights of testis and epididymis but not the seminal vesicle weight.The length and frequency of stages IX-XIV seminif-erous tubules,where mitosis occurred,were increased and stages I-VI were reduced in rats treated with Lepidiummeyenii.Conclusion;The Lepidium meyenii root invigorates spermatogenesis in male rats by acting on its initialstages(IX-XIV). 展开更多
关键词 spermatogenesis Lepidium meyenii MACA TRANSILLUMINATION
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Expression and localization of CKLFSF2 in human spermatogenesis 被引量:16
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作者 Gang Liu Zhong-Cheng Xin +5 位作者 Liang Chen Long Tian Yi-Ming Yuan Wei-Dong Song Xue-Jun Jiang Ying-Lu Guo 《Asian Journal of Andrology》 SCIE CAS CSCD 2007年第2期189-198,共10页
Aim: To investigate the expression and subcellular localization of chemokine-like factor superfamily 2 (CKLFSF2) in human testis and its potential role in spermatogenesis. Methods: A specific polyclonal antibody a... Aim: To investigate the expression and subcellular localization of chemokine-like factor superfamily 2 (CKLFSF2) in human testis and its potential role in spermatogenesis. Methods: A specific polyclonal antibody against CKLFSF2 was raised. The expression and cellular localization of CKLFSF2 in the seminiferous tubules was checked by immunohistochemistry method. Also, in situ hybridization was applied to localize the mRNA distribution. The EGFP- CKLFSF2 fusion protein was expressed in COS-7 cells to localize its subcellular location in vitro. In addition, the abnormal expression of CKLFSF2 in testes of patients with male infertility was assayed by reverse transcription polymerase chain reaction (RT-PCR) and immunohistochemistry methods. Results: Having a close correlation with spermatogenesis defects, CKLFSF2 was specifically expressed in meiotic and post-meiotic germ cells, which were localized to the endoplasmic reticulum (ER) near the Golgi apparatus. Conclusion: CKLFSF2 could play important roles in the process of meiosis and spermiogenesis, and might be involved in the vesicular transport or membrane apposition events in the endoplasmic reticulum. 展开更多
关键词 spermatogenesis TESTIS chemokine-like factor superfamily INFERTILITY endoplasmic reticulum
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Blood-testis barrier and spermatogenesis: lessons From genetically-modified mice 被引量:9
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作者 Xiao-Hua Jiang Ihtisham Bukhari +4 位作者 Wei Zheng Shi Yin Zheng Wang Howard J Cooke Qing-Hua Shi 《Asian Journal of Andrology》 SCIE CAS CSCD 2014年第4期572-580,共9页
The blood-testis barrier (BTB) is found between adjacent Sertoli cells in the testis where it creates a unique microenvironment for the development and maturation of meiotic and postmeiotic germ cells in seminiferou... The blood-testis barrier (BTB) is found between adjacent Sertoli cells in the testis where it creates a unique microenvironment for the development and maturation of meiotic and postmeiotic germ cells in seminiferous tubes. It is a compound proteinous structure, composed of several types of cell junctions including tight junctions (TJs), adhesion junctions and gap junctions (GJs). Some of the junctional proteins function as structural proteins of BTB and some have regulatory roles. The deletion or functional silencing of genes encoding these proteins may disrupt the BTB, which may cause immunological or other damages to meiotic and postmeiotic cells and ultimately lead to spermatogenic arrest and infertility. In this review, we will summarize the findings on the BTB structure and function from genetically-modified mouse models and discuss the future perspectives. 展开更多
关键词 blood-testis barrier genetically-modified mouse seminiferous tubule sertoli cells spermatogenesis
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Effect of vasectomy via inguinal canal on spermatogenesis in rabbits 被引量:8
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作者 Bin Peng Ya-Ping Wang +2 位作者 Yi Shang Yang Guo Zheng-Wei Yang 《Asian Journal of Andrology》 SCIE CAS CSCD 2008年第3期486-493,共8页
Aim: To determine whether vasectomy away from the epididymal tail (via the inguinal canal) in rabbits can reduce the early postoperative effects on spermatogenesis. Methods: Twenty-nine normal male Japanese white ... Aim: To determine whether vasectomy away from the epididymal tail (via the inguinal canal) in rabbits can reduce the early postoperative effects on spermatogenesis. Methods: Twenty-nine normal male Japanese white rabbits (aged 4- 6 months) were subjected to unilateral close-ended (conventional) or open-ended (the cut end of the juxta-epididymal vas deferens not ligated) vasectomy via the inguinal canal. Ten days and 3 months after operation, testes, epididymides and vasa deferentia were removed and methacrylate resin-embedded sections prepared. The histology of the testis, epididymis and vas deferens was examined under light microscope, and the volume and diameter of the seminiferous tubules were quantitatively studied using stereological methods. Results: Neither of the methods of vasectomy led to apparent damage to spermatogenesis on the vasectomized side in comparison with the contralateral shamoperated side, but the juxta-epididymal vas deferens on the vasectomized side was highly distended and contained numerous sperm 3 months after operation. Conclusion: Vasectomy away from the cauda epididymis has no significant early postoperative effects on spermatogenesis in rabbits. 展开更多
关键词 VASECTOMY inguinal canal spermatogenesis TESTIS RABBITS
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Preliminary study of letrozole use for improving spermatogenesis in non-obstructive azoospermia patients with normal serum FSH 被引量:9
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作者 Giorgio Cavallini Giovanni Beretta Giulio Biagiotti 《Asian Journal of Andrology》 SCIE CAS CSCD 2011年第6期895-897,共3页
We investigated whether letrozole (2.5 mg day-1) improves sperm count in non-obstructive azoospermia (NOA) patients. Four men were included in this study, and they had folliculo-stimulating hormone and other hormo... We investigated whether letrozole (2.5 mg day-1) improves sperm count in non-obstructive azoospermia (NOA) patients. Four men were included in this study, and they had folliculo-stimulating hormone and other hormone levels within the normal range and no varicoceles or chromosomal aberrations. These four patients were administered letrozole for 3 months. Sperm count, testicular volume, gonadotropin, testosterone (T) and estradiol (E2) blood levels were assessed before, during and 1 week after the suspension of treatment. All patients showed spermatozoa in their ejaculate, increased gonadotropin and T levels and lower E2 levels (P〈0.05 in all cases), when letrozole was administered. This suggests that letrozole treatment might improve sperm count in an NOA sub-population; however, more studies, including the proper controls, are needed to confirm its efficacy. 展开更多
关键词 LETROZOLE medical treatment non-obstructive azoospermia spermatogenesis
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Radio-protective effect of vitamin E on spermatogenesis in mice exposed to γ-irradiation: a flow cytometric study 被引量:7
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作者 C.Songthaveesin J.Saikhun +1 位作者 Y.Kitiyanant K.Pavasuthipaisit 《Asian Journal of Andrology》 SCIE CAS CSCD 2004年第4期331-336,共6页
Aim: To investigate the effect of vitamin E on the radioprotection of spermatogenesis and chromatin condensation of spermatozoa during passage through the epididymis in mice exposed to irradiation. Methods: Adult outb... Aim: To investigate the effect of vitamin E on the radioprotection of spermatogenesis and chromatin condensation of spermatozoa during passage through the epididymis in mice exposed to irradiation. Methods: Adult outbred male ICR mice were orally administered natural vitamin E (VE, D-α-tocopheryl acetate) at 400 IU/kg for 7 days before exposure to 1 Gy of γ-irradiation. The animals were sacrificed at day 1, 7, 14, 21, 28, 35 and 70 post-irradiation (IR) and the percentage of testicular germ cells and epididymal sperm chromatin condensation was analyzed using flow cytometry. Results: Serum D-α-tocopheryl acetate levels were 47.4 ± 3.2 μg/dL in the treated group, yet it could not be detected in the control group. The testicular weight of irradiated mice pretreated with VE+IR was significantly (P<0.05) higher than that of those without VE treatment (IR) at day 14 and 21 post-irradiation. The percentage of primary spermatocytes (4C) in the VE+IR group was comparable to the controls but significantly (P<0.05) higher than those in the IR group from day 7 to 35 post-irradiation. The percentage of round spermatids (1C) in the VE+IR group was also significantly (P<0.05) higher than those in the IR group at day 28 post-irradiation. The primary spermatocytes : spermatogonia ratio in the IR group was significantly (P<0.05) declined at day 7 to 35 post-irradiation when compared to the VE+IR and control groups. The round spermatid : spermatogonia ratio in the VE+IR group was significantly (P<0.05) higher than that of the IR group at day 14 and 28 post-irradiation. The chromatin condensation of epididymal spermatozoa measured by propidium iodide uptake was not affected by 1 Gy of γ-irradiation. Conclusion: The administration of VE prior to irradiation protects spermatogenic cells from radiation. 展开更多
关键词 vitamin E RADIOPROTECTION spermatogenesis mouse flow cytometry
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Proteomics of spermatogenesis: from protein lists to understanding the regulation of male fertility and infertility 被引量:7
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作者 Xiaoyan Huang Jiahao Sha 《Asian Journal of Andrology》 SCIE CAS CSCD 2011年第1期18-23,共6页
Proteomic technologies have undergone significant development in recent years, which has led to extensive advances in protein research. Currently, proteomic approaches have been applied to many scientific areas, inclu... Proteomic technologies have undergone significant development in recent years, which has led to extensive advances in protein research. Currently, proteomic approaches have been applied to many scientific areas, including basic research, various disease and malignant tumour diagnostics, biomarker discovery and other therapeutic applications. In addition, proteomics-driven research articles examining reproductive biology and medicine are becoming increasingly common. The key challenge for this field is to move from lists of identified proteins to obtaining biological information regarding protein function. The present article reviews the available scientific literature related to spermatogenesis. In addition, this study uses two-dimensional electrophoresis mass spectrometry (2DE-MS) and liquid chromatography (LC)-MS to construct a series of proteome profiles describing spermatogenesis. This large-scale identification of proteins provides a rich resource for elucidating the mechanisms underlying male fertility and infertility. 展开更多
关键词 INFERTILITY male fertility PROTEOMICS spermatogenesis
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The roles of microRNAs in regulation of mammalian spermatogenesis 被引量:7
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作者 Xiaoxu Chen Xueliang Li +2 位作者 Jiayin Guo Pengfei Zhang Wenxian Zeng 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2017年第4期779-786,共8页
Mammalian spermatogenesis contains three continuous and organized processes, by which spermatogonia undergo mitosis and differentiate to spermatocytes, follow on meiosis to form haploid spermatids and ultimately trans... Mammalian spermatogenesis contains three continuous and organized processes, by which spermatogonia undergo mitosis and differentiate to spermatocytes, follow on meiosis to form haploid spermatids and ultimately transform into spermatozoa. These processes require an accurately, spatially and temporally regulated gene expression patterns. The microRNAs are a novel class of post-transcriptional regulators. Cumulating evidences have demonstrated that microRNAs are expressed in a cell-specific or stage-specific manner during spermatogenesis. In this review, we focus on the roles of microRNAs in spermatogenesis. We highlight that N6-methyladenosine(m6A)is involved in the biogenesis of microRNAs and miRNA regulates the m6A modification on mRNA, and that specific mi RNAs have been exploited as potential biomarkers for the male factor infertility, which will provide insightful understanding of microRNA roles in spermatogenesis. 展开更多
关键词 MICRORNAS RNA METHYLATION spermatogenesis
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The role of C-type natriuretic peptide in rat testes during spermatogenesis 被引量:7
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作者 Dong-Hui Huang Shi-Wei Zhang +1 位作者 Hu Zhao Ling Zhang 《Asian Journal of Andrology》 SCIE CAS CSCD 2011年第2期275-280,共6页
C-type natriuretic peptide (CNP) is a 22-amino acid peptide and act as a local paracrine or autocrine regulator. There is growing evidence that ChIP is involved in male reproductive processes. To investigate the rol... C-type natriuretic peptide (CNP) is a 22-amino acid peptide and act as a local paracrine or autocrine regulator. There is growing evidence that ChIP is involved in male reproductive processes. To investigate the role of CNPduring spermatogenesis, we measured the mRNA expression of CNPand its specific membrane-bound natriuretic peptide receptor-B (NPR-B) using real-time RT-PCR in the testes of normal rats on different postnatal days. After that spermatogenesis dysfunction model induced by ornidazole was established with the aim to study the correlation of CNPwith spermatogenic dysfunction. Then, Sertoli cells from 18- to 22-day-old healthy male rats were cultured in the presence of different CNPconcentrations (1 ×10-6, 1×10-7 and1×10-8 mol l-1), and the mRNA expression levels of androgen.binding protein, inhibin B and transferrin were examined at 0 min, 30 min, 1 h, 2 h, 4 h, 8 h, 12 h, 24 h and 48 h. During the postnatal development of rat testes, the highest mRNA expression levels of CNPand NPR-B were found at postnatal Do, and the levels then declined gradually, with a second ChIPpeak at postnatal D35. In the ornidazole-induced infertile rat testes, CIVPgene expression was lower than in the uninduced rats (P〈0.05), while IVPR-Bgene expression was greater (P〈0.05). In cultured Sertoli cells, supplementation with CNP stimulated the gene expression of androgen-binding pmteirginhibin B/transferrin, particularly at 12 h, and 1× 10-7 mol l-1 CNP had the highest upregulation effect. The gene expression levels of CNPIIVPR-B in rat testes at different postnatal stages and in infertile rat testes indicated that CNP may participate in the physiology and/or pathology related to spermatogenesis. Moreover, ChIP regulated endocrine function in Sertoli cells. Taken together, these results showed that CNP is closely tied to spermatogenesis. 展开更多
关键词 C-type natriuretic peptide natriuretic peptide receptor-B Sertoli cell culture spermatogenesis
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Reversible effect of testosterone undecanoate injection on spermatogenesis in rats 被引量:6
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作者 Xiao-HongWEN Wing-HaiWANG +2 位作者 Jian-SunTONG ZHENG-WeiYANG Gui-YuanZHANG 《Asian Journal of Andrology》 SCIE CAS CSCD 2000年第3期207-211,共5页
Aim: To study the effect of testosterone undecanoate (TU) injection on spermatogenesis in rats. Methods:Twenty adult SD rats received vehicle or TU (8 mg/kg, 19 mg/kg or 625 mg/kg) injection, im, every 15 days for 60d... Aim: To study the effect of testosterone undecanoate (TU) injection on spermatogenesis in rats. Methods:Twenty adult SD rats received vehicle or TU (8 mg/kg, 19 mg/kg or 625 mg/kg) injection, im, every 15 days for 60days, and another 38 animals received similar treatments for 130 days with half of them undergoing a recovery phase of120 days (5 rats for each treatment). At the end of the treatment, testes were removed and the diameter of the seminif-erous tubules and the number of late elongated spermatids (steps 15-19) per testis were estimated with stereologicalmethods as a measure of the spermatogenic efficiency. Results: Low dose (8 mg/kg) TU treatment virtually hadno effect on spermatogenesis. A dose of 19 mg/kg slightly suppressed spermatogenesis 60 days after treatment, and se-vere suppression occurred after another 70 days of dosing. Spermatogenesis was completely recovered at the end of therecovery phase. Large dose (625 mg/kg) TU treatment did not significantly affect spermatogenesis and was well toler-ated by animals. Conclusion: TU injection reversibly suppresses spermatogenesis in rats. 展开更多
关键词 spermatogenesis STEREOLOGY TESTIS testosterone undecanoate
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