EST isozymes are one of the frequently used biochemical markers in genetic analysis of fungi. and the staining is an important process in electrophoresis analysis of studying Est.The effects were compared ainong diff...EST isozymes are one of the frequently used biochemical markers in genetic analysis of fungi. and the staining is an important process in electrophoresis analysis of studying Est.The effects were compared ainong different stain recipes for Est of 3 kinds of fungi-Lentinus edodes. Pleurotus sapidus. Phellinus igriarius and 2 kinds of plants-Populus sp and Brassica chinensis. Of the four kinds of Est staining recipes tested.the recipe α-acetic acid-naphther showed the best effect.and followed by β-aceticacid-naphther, semicontent α-aceticacid-naphther and α+β-aceticacidnathpher.展开更多
Egg galleries of Tomicus piniperda attacking Pinus yunnanensis were investigated during different period of trunk attack in mid Yunnan Province. 30 Genus of fungi,which belong to 12 families,were isolated. Among them,...Egg galleries of Tomicus piniperda attacking Pinus yunnanensis were investigated during different period of trunk attack in mid Yunnan Province. 30 Genus of fungi,which belong to 12 families,were isolated. Among them,12 are saprophytes,10 facultative fungi and 8 parasites. Moreover,there is no obvious differentiation in the dominant populations including Debaryomyces,Penicillium and Paecilomyces with the damage level of localities and there exists no close interior relation between bark beetle attacks and dominant populations. Besides these,blue stain fungi which identified as Leptographium spp.were isolated from Tomicus piniperda and trunk tunnels.展开更多
文摘EST isozymes are one of the frequently used biochemical markers in genetic analysis of fungi. and the staining is an important process in electrophoresis analysis of studying Est.The effects were compared ainong different stain recipes for Est of 3 kinds of fungi-Lentinus edodes. Pleurotus sapidus. Phellinus igriarius and 2 kinds of plants-Populus sp and Brassica chinensis. Of the four kinds of Est staining recipes tested.the recipe α-acetic acid-naphther showed the best effect.and followed by β-aceticacid-naphther, semicontent α-aceticacid-naphther and α+β-aceticacidnathpher.
文摘Egg galleries of Tomicus piniperda attacking Pinus yunnanensis were investigated during different period of trunk attack in mid Yunnan Province. 30 Genus of fungi,which belong to 12 families,were isolated. Among them,12 are saprophytes,10 facultative fungi and 8 parasites. Moreover,there is no obvious differentiation in the dominant populations including Debaryomyces,Penicillium and Paecilomyces with the damage level of localities and there exists no close interior relation between bark beetle attacks and dominant populations. Besides these,blue stain fungi which identified as Leptographium spp.were isolated from Tomicus piniperda and trunk tunnels.