Taking tumorous stem mustard infected by black spot disease as the research material, the ribosomal 5.8S rDNA and its flanking ITS region were cloned, sequenced and aligned in the study. The results showed that the ba...Taking tumorous stem mustard infected by black spot disease as the research material, the ribosomal 5.8S rDNA and its flanking ITS region were cloned, sequenced and aligned in the study. The results showed that the base sequence of pathogens collected from five different sites was almost identical with that of Alternaria brassicae, and the similarity degree reached 99.68%, without base difference greater than 3 bp. It had obvious differences with the sequences of A. brassicicola and A. japonica, and the base difference was greater than 3 bp with a lot of fragment deletions. It was preliminarily determined that the pathogen cau- sing black spot disease on tumorous stem mustard was A. brassicae.展开更多
背景:近些年,肝脏类器官的发展使其成为国际肝病研究领域的热点,但目前仍未有文献对其进行文献计量学分析。目的:基于文献计量学与可视化分析探索近20年肝脏类器官的热点趋势。方法:从Web of Science(科学网,WOS)核心合集中检索2002-01-...背景:近些年,肝脏类器官的发展使其成为国际肝病研究领域的热点,但目前仍未有文献对其进行文献计量学分析。目的:基于文献计量学与可视化分析探索近20年肝脏类器官的热点趋势。方法:从Web of Science(科学网,WOS)核心合集中检索2002-01-01/2022-11-12肝脏类器官的相关文献,运行Origin、Office和CiteSpace软件进行文献计量与可视化分析,通过生成图表的方式来统计分析文献的年发文量、国家、机构、作者、期刊和关键词等内容。结果与结论:肝脏类器官研究领域近20年的发文量、被引频次、加入研究的机构和人员总体呈现上升趋势,说明该领域发展迅速关注度也逐渐升高。在该领域中,美国的发文量最多、影响力最强,虽然投入大量的时间与精力,但是在众多研究机构中美国单个研究机构的发文量并非最高;中国发文量仅次于美国,中国科学院和复旦大学是国内发文量最多的机构。荷兰乌得勒支大学是发文量最多的机构,发文量最高的作者是Clevers H,共引频次最高的文章是“Long-term culture of genome-stable bipotent stem cells from adult human liver”,分子学、生物学、免疫学是肝脏类器官研究的主流学科,出现次数最高的关键词包括stem cell(干细胞)、in vitro(体外)和culture(培养),肝脏类器官领域的研究热点主要集中在体外干细胞三维培养、分化及基因表达。展开更多
基金Supported by Science and Technology Research Project of Chongqing Municipal Education Commission(KJ111307)
文摘Taking tumorous stem mustard infected by black spot disease as the research material, the ribosomal 5.8S rDNA and its flanking ITS region were cloned, sequenced and aligned in the study. The results showed that the base sequence of pathogens collected from five different sites was almost identical with that of Alternaria brassicae, and the similarity degree reached 99.68%, without base difference greater than 3 bp. It had obvious differences with the sequences of A. brassicicola and A. japonica, and the base difference was greater than 3 bp with a lot of fragment deletions. It was preliminarily determined that the pathogen cau- sing black spot disease on tumorous stem mustard was A. brassicae.
文摘背景:近些年,肝脏类器官的发展使其成为国际肝病研究领域的热点,但目前仍未有文献对其进行文献计量学分析。目的:基于文献计量学与可视化分析探索近20年肝脏类器官的热点趋势。方法:从Web of Science(科学网,WOS)核心合集中检索2002-01-01/2022-11-12肝脏类器官的相关文献,运行Origin、Office和CiteSpace软件进行文献计量与可视化分析,通过生成图表的方式来统计分析文献的年发文量、国家、机构、作者、期刊和关键词等内容。结果与结论:肝脏类器官研究领域近20年的发文量、被引频次、加入研究的机构和人员总体呈现上升趋势,说明该领域发展迅速关注度也逐渐升高。在该领域中,美国的发文量最多、影响力最强,虽然投入大量的时间与精力,但是在众多研究机构中美国单个研究机构的发文量并非最高;中国发文量仅次于美国,中国科学院和复旦大学是国内发文量最多的机构。荷兰乌得勒支大学是发文量最多的机构,发文量最高的作者是Clevers H,共引频次最高的文章是“Long-term culture of genome-stable bipotent stem cells from adult human liver”,分子学、生物学、免疫学是肝脏类器官研究的主流学科,出现次数最高的关键词包括stem cell(干细胞)、in vitro(体外)和culture(培养),肝脏类器官领域的研究热点主要集中在体外干细胞三维培养、分化及基因表达。
