The intrinsic developmental age signal defines an age-dependent climbing behavior in cucumber

The tendril is a climbing organ in cucurbits and functions in physical support and to avoid shading by neighboring vegetation.However,how cucurbits produce tendrils to obtain climbing ability is largely unknown.In this study,tendril phenotypes were investigated during different developmental stages.Our results revealed that tendril growth exhibited an age-dependent pattern in cucurbits.Tendril growth was inhibited,and the tendril was formed as a short tendril[nonfunctional tendril(nonF-tendril),approximately 0.1 cm]during the seedling stage.In contrast,enhanced cell proliferation and cell expansion led to rapid elongation of the tendril during the climbing stage,and the tendril formed as a functional tendril(F-tendril,approximately 30 cm)to obtain climbing ability.RT-qPCR detection showed that age-dependent tendril growth correlated negatively with the abundance of the conserved age regulator CsmiR156.Defoliation induced CsmiR156 to inhibit CsSPLs,and F-tendril formation and climbing ability were delayed in defoliated cucumbers,which confirmed the role of CsmiR156 in regulating tendril growth in vivo.Additionally,exogenous gibberellin(GA)treatment showed that GA positively regulated tendril growth,and RT-qPCR detection showed that the GA bio-synthetic genes and metabolic genes were affected by age pathway,suggesting that the age pathway depended on GA bio-synthetic and metabolic pathway to regulate cell expansion to determine tendril growth.In summary,our work reveals that change in tendril type is an important marker of phase transition in cucumber,and tendril growth is regulated by an intrinsic developmental age signal,ensuring that the cucumber obtains climbing ability at a suitable age.

Spatial heterogeneity and driving mechanism of urban climbing and its impact on regional environment in varied landform types in the middle reaches of the Yellow River

Urbanization research is essential for the sustainable use of regional land resources and ecological environment protection.The expansion process and driving factors of urban construction land at different scales in the middle reaches of the Yellow River(MRYR)have not been comprehensively elucidated.In this study,we explored the distribution pattern of urban construction land on different slope gradients at different scales and analyzed its influencing factors.The main findings were as follows:(1)There has been significant expansion of urban construction land in the MRYR over the past 20 years.Spatial heterogeneity was observed in the regional urban construction land expansion process among different geomorphic regions.(2)The urban construction land in the MRYR was expanded vertically to areas with slopes of>5°,particularly in 2005–2010.Significant slope climbing of urban construction land was observed in the loess hilly-gully and rocky mountain areas.(3)In MRYR,68.45%of the counties were categorized as the slope-climbing types,including 37.38%high-slope-climbing types.(4)The regional population density and economic development level were closely associated with regional urban construction land area variability.(5)The climbing process of regional urban construction can effectively alleviate farmland encroachment and pressure on the regional ecological environment.The urban expansion of the metropolitan distribution areas in the Plain region(such as Xi'an,Taiyuan)had a relatively significant impact on the local carbon storage.

Stair climbing,genetic predisposition,and the risk of incident type 2 diabetes:A large population-based prospective cohort study

Background:Cross-sectional evidence and small-scale trials suggest positive effects of stair climbing on cardiometabolic disease and glucose regulation.However,few studies have examined the long-term association between stair climbing and the incidence of type 2 diabetes(T2D).We aimed to prospectively evaluate the association of stair climbing with T2D and assess modifications by genetic predisposition to T2D.Methods:We included 451,699 adults(mean age=56.3±8.1 years,mean±SD;55.2%females)without T2D at baseline in the UK Biobank and followed up to March 31,2021.Stair climbing information was collected through the touchscreen questionnaire.Genetic risk score for T2D consisted of 424 single nucleotide polymorphisms.Results:During a median follow up of 12.1 years,14,896 T2D cases were documented.Compared with participants who reported no stair climbing,those who climbed stairs regularly had a lower risk of incident T2D(10-50 steps/day:hazard ratio(HR)=0.95,95%confidence interval(95%CI):0.89-1.00;60-100 steps/day:HR=0.92,95%CI:0.87-0.98;110-150 steps/day:HR=0.86,95%CI:0.80-0.91;>150 steps/day:HR=0.93,95%CI:0.87-0.99,p for trend=0.0007).We observed a significant interaction between stair climbing and genetic risk score on the subsequent T2D risk(p for interaction=0.0004),where the risk of T2D showed a downward trend in subjects with low genetic risk and those who reported stair climbing activity of 110-150 steps/day appeared to have the lowest overall T2D risk among those with intermediate to high genetic risk.Conclusion:A higher number of stairs climbed at home was associated with lower T2D incidence risk,especially among individuals with a low genetic predisposition to T2D.These findings highlight that stair climbing,as incidental physical activity,offers a simple and low-cost complement to public health interventions for T2D prevention.

Investigation on the Effect of the Tension Degree of Tracks on the Adsorption Performance of Tracked Wall Climbing Robot

In this paper,a new tracked wall climbing robot with permanent magnetic units is designed,and the tension degree of robot’s tracks is found to have a significant impact on the robot’s adsorption performance.This tracked wall climbing robot is a remotely controlled robot.All the control devices can be installed on the robot body.All the permanent magnetic units are arranged on the light track.In order to illustrate the relationship between the tension degree and the adsorption performance,when absorbed on the vertical surface and 180⁃degree inverted surface,the static force analysis of the robot is presented.Finally,experiments were demonstrated to prove that higher tension degree of tracks can make adsorption performance better.

Efficient Clustering Using Memetic Adaptive Hill Climbing Algorithm in WSN

Wireless Sensor Networks are composed of autonomous sensing devices which are interconnected to form a closed network.This closed network is intended to share sensitive location-centric information from a source node to the base station through efficient routing mechanisms.The efficiency of the sensor node is energy bounded,acts as a concentrated area for most researchers to offer a solution for the early draining power of sensors.Network management plays a significant role in wireless sensor networks,which was obsessed with the factors like the reliability of the network,resource management,energy-efficient routing,and scalability of services.The topology of the wireless sensor networks acts dri-ven factor for network efficiency which can be effectively maintained by perform-ing the clustering process effectively.More solutions and clustering algorithms have been offered by various researchers,but the concern of reduced efficiency in the routing process and network management still exists.This research paper offers a hybrid algorithm composed of a memetic algorithm which is an enhanced version of a genetic algorithm integrated with the adaptive hill-climbing algorithm for performing energy-efficient clustering process in the wireless sensor networks.The memetic algorithm employs a local searching methodology to mitigate the premature convergence,while the adaptive hill-climbing algorithm is a local search algorithm that persistently migrates towards the increased elevation to determine the peak of the mountain(i.e.,)best cluster head in the wireless sensor networks.The proposed hybrid algorithm is compared with the state of art clus-tering algorithm to prove that the proposed algorithm outperforms in terms of a network life-time,energy consumption,throughput,etc.

Design and analysis of a bisected wheel-based cable climbing robot

To inspect inner wires of the cylindrical cables on a cable-stayed bridge, a new bisected wheel-based cable climbing robot is designed. The simple structure and the moving mode are described and the static features of the robot are analyzed. A cable with a diameter of 139 mm is selected as an example to calculate the design parameters of the robot. For safety energysaving landing in the case of electrical system failure, an electric damper based on back electromotive force and a gas damper with a slider-crank mechanism are introduced to exhaust the energy generated by gravity when the robot is slipping down along the cables. A simplified mathematical model is analyzed and the landing velocity is simulated. For the present design, the robot can climb up a cable with diameters varying from 65 to 205 mm with payloads below 3.5 kg. Several climbing experiments performed on real cables confirm that the proposed robot meets the demands of inspection.

Determination of the Content of Total Alkaloids in Different Solvent Extracts from Climbing Groundsel Herb Produced in Guizhou Province and Their Antibacterial Activity

[Objective] This study aimed to compare the contents of total alkaloids in different solvent extracts from climbing groundsel herb produced in Guizhou Province and their antibacterial activity. [Method] The content of total alkaloids was measured by UV spectrophotometry, and Oxford cups were used to investigate the antibacterial activity of each solvent extract. [Result] There was a good linear correlation between the absorbance measured by the UV spectrophotometer and the content of total al- kaloids within the concentration of 0.011 0-0.054 8 mg/ml at 207 nm, and the re- gression equation was Y=23.654X＋0.021, R=0. 999 7 and the average recovery rate was 99.2%. The contents of total alkaloids in 60% ethanol extract, 95% ethanol and water ethanol were 38.71, 52.25 and 60.50 mg/g, respectively. The 60% ethanol ex- tract had strong antibacterial activity against Streptococcus pneumoniae, Staphylo- coccus aureus and Escherichia coil; the water extractive had stronger antibacterial activity against Escherichia colr, 95% ethanol extract had weak antibacterial activity, with no inhibition of Pseudomonas aeruginosa. [Conclusion] This method is accurate, simple, with good repeatability, which can be used for the determination of alkaloids content of the climbing groundsel herb; there is no positive correlation between the content of total alkaloids in different solvent extracts of climbing groundsel herb and their antibacterial activity.

A Biomimetic Climbing Robot Based on the Gecko

The excellent climbing performance of the gecko is inspiring engineers and researchers for the design of artificial systems aimed at moving on vertical surfaces. Climbing robots could perform many useful tasks such as surveillance, inspection, repair, cleaning, and exploration. This paper presents and discusses the design, fabrication, and evaluation of two climbing robots which mimic the gait of the gecko. The first robot is designed considering macro-scale operations on Earth and in space. The second robot, whose motion is controlled using shape memory alloy actuators, is designed to be easily scaled down for micro-scale applications. Proposed bionic systems can climb up 65 degree slopes at a speed of 20 mm·s^-1.

Multi-Scale Compliant Foot Designs and Fabrication for Use with a Spider-Inspired Climbing Robot

Climbing robots are of potential use for surveillance, inspection and exploration in different environments. In particular, the use of climbing robots for space exploration can allow scientists to explore environments too challenging for traditional wheeled designs. To adhere to surfaces, biomimetic dry adhesives based on gecko feet have been proposed. These biomimetic dry adhesives work by using multi-scale compliant mechanisms to make intimate contact with different surfaces and adhere by using Van der Waals forces. Fabrication of these adhesives has frequently been challenging however, due to the difficulty in combining macro, micro and nanoscale compliance. We present an all polymer foot design for use with a hexapod climbing robot and a fabrication method to improve reliability and yield. A high strength, low-modulus silicone, TC-5005, is used to form the foot base and microscale fibres in one piece by using a two part mold. A macroscale foot design is produced using a 3D printer to produce a base mold, while lithographic definition of microscale fibres in a thick photoresist forms the ＇hairs＇ of the polymer foot. The adhesion of the silicone fibres by themselves or attached to the macro foot is examined to determine best strategies for placement and removal of feet to maximize adhesion. Results demonstrate the successful integration of micro and macro compliant feet for use in climbing on a variety of surfaces.

Critical Suction Characteristic Analyses of a Wall Climbing Robot

A new method called critical suction is used based on the wall climbing robot demands of miniature structure, moving smartly and low noise. It makes the robot achieve the homeostasis state in the suction cup, and in this condition the robot can stay on the wall reliably and move smartly. The fluid mechanics model and fluid network model are set up to analyze the robot suction system when the airflow is steady or changes suddenly. Furthermore, simulation results indicate the close relation between the key parameters of robot structure and the suction system. Finally the method of critical suction proves correct in theory.

Trial mountain climbing algorithm for solving the inverse kinematics of redundant manipulator

Trial mountain climbing algorithm to solve the inverse kinematics problem of redundant manipulator is introduced, and a method of describing a numeral with a special numeration system is given to define the changed step of the trail mountain climbing algorithm. The results show that a likelihood solution can be found quickly in the infinite groups of likelihood solutions within the limited search times, and need not calculate the anti trigonometric function and the inverse matrix. In addition, this algorithm has many good qualities such as concise algorithm, tiny computation, fast convergence velocity, good stability and extensive adaptability.

On Maximal Weight Moment and Overturn Tendency of Multi-Sucker Wall-Climbing Mechanism

For a multi legged robot climbing on a vertical wall, how to increase its anti overturn capacity (AOC) and to detect overturn tendency are most important. They form the basis of off/on line gait programming and safety monitoring system. In the paper, after investigating the indeterminate statics of an insect like six legged wall climbing mechanism in various support patterns, the authors obtained the analytical expressions of maximal weight moment with respect to different support patterns, and the overturn tendency while payload was increased. As a side product, the authors find an unusual phenomenon governing the relationship between the robot's AOC and the number of functioning suckers, which is explained theoretically by distributed mass spring statics model.

Woody Climbing Plants and Their Landscaping Application in Chongqing City

There are a variety of woody climbing plants distributed widely in Chongqing City, and they are loved by landscape designers due to their special form and saving space. In respect of actual application,there is a big difference between various species of woody climbing plants in terms of proportion. In this paper, woody climbing plants in Chongqing City were investigated. The results show that there are74 species of woody climbing plants suitable for landscape in Chongqing City. The research can provide reference for the application of woody climbing plants in landscape greening in Chongqing City in future.

Macroscopic and microscopic mechanical behaviors of climbing tendrils

Tendril-bearing climbing plants must recur to the tendril helices with chiral perversion or dual chirality for climbing and to obtain sun exposure. Despite researchers' prolonged fascination with climbing tendrils since Darwin's time and even earlier, why the soft and slender tendrils can bear heavy loads such as the self-weight of a plant or additional load caused by rain remains elusive. In this paper, we take towel gourd tendrils as an example and investigate the macroscopic and microscopic mechanical behaviors of tendrils through experiments and simulations. Our study indicates that the tendril filament exhibits rubber-like hyperelastic behaviors and can particularly endure large elongation, which is mainly attributed to the superelasticity of the cellulose fibril helix contained in the cell wall. Combination of the tendril helical structure with dual chirality or chiral perversion at a macroscale and a cellulose filament helix at a subcellular level creates superior elasticity for biological species relying on support and climbing. This study provides deep insight into the structure-property relationship of climbing tendrils, and the relationship is useful for the bioinspired design of composite systems with superior elasticity.

Research on adsorption mechanism of wall climbing robots based on internally balanced theory

The internally balanced theory proposed by the Japanese researchers,solved the contradiction between adsorption ability and moving capability of the permanent magnetic adsorption mechanism.However,it still has some problems when applied to wall climbing robots.This paper analyzes and improves this theory,and the improved internally balanced theory satisfies the requirements of the adsorption mechanism significantly.Finally,a practical prototype is proposed based on this method,and both the analysis using ANSYS and the experiment results justify the design validity.

Adsorption Performance of Sliding Wall-Climbing Robot

Sliding wall-climbing robot （SWCR） is applied worldwide for its continuous motion, however, considerable air leakage causes two problems： great power consumption and big noise, and they constraint the robot＇s comprehensive performance. So far, effective theoretical model is still lacked to solve the problems. The concept of SWCR＇s adsorption performance is presented, and the techniques of improving utilization rate of given adsorption force and utilization rate of power are studied respectively to improve SWCR＇s adsorption performance. The effect of locomotion mechanism selection and seal＇s pressure allocation upon utilization rate of given adsorption force is discussed, and the theoretical way for relevant parameters optimization are provided. The directions for improving utilization rate of power are pointed out based on the detail analysis results of suction system＇s thermodynamics and hydrodynamics. On this condition, a design method for SWCR-specific impeller is presented, which shows how the impeller＇s key parameters impact its aerodynamic performance with the aid of computational fluid dynamics （CFD） simulations. The robot prototype, BIT Climber, is developed, and its functions such as mobility, adaptability on wall surface, payload, obstacle ability and wall surface inspection are tested. Through the experiments for the adhesion performance of the robot adsorption system on the normal wall surface, at the impeller＇s rated rotating speed, the total adsorption force can reach 237.2 N, the average effective negative pressure is 3.02 kPa and the design error is 3.8% only, which indicates a high efficiency. Furthermore, it is found that the robot suction system＇s static pressure efficiency reaches 84% and utilization rate of adsorption force 81% by the experiment. This thermodynamics model and SWCR-specific impeller design method can effectively improve SWCR＇s adsorption performance and expand this robot applicability on the various walls. A sliding wall-climbing robot with high adhesion efficiency is developed, and this robot has the features of light body in weight, small size in structure and good capability in payload.

The Kinematics and Force Analysis of a New Leg Mechanism for Multi-legged Wall-Climbing Robot

This paper presents a new kind of leg mechanism with which the wall climbing robot can easily perform the ground to wall transition by itself.To get its walking envelope and limit position,the forward/inverse kinematics and the statics of the mechanism are solved.All of these lay the foundation for ground to wall transition gait programing,mechanism parameter selection and optimization.

A Wall Climbing Robot with Two Driven Wheels

A wall climbing robot with two driven wheels and single adhering disk is introduced in this paper. The robot has a compact structure and can be controlled flexibly. This kind of robot will have a wide prospect for application.

PERFORMANCE OF A KIND OF PERMANENT MAGNETICSUCKERSUSED IN WALL- CLIMBING ROBOTS

The magnetic circuit of a kind of permanent magnetic sucker attached to the tracks of a wall climbing robot was researched. The formula of the attractive force of sucker to a wall was derived and the relationship between the force and the air gaps was analyzed. Furthermore the effect of the parameters of the magnetic sucker on the sucker’s performance was discussed. The experiments show that proper selections of the sucker’s structural parameters can provide sufficient attractive force so as to make the wall climbing robot move safely on the steel wall surface.

Wall-climbing Robot with Negative Pressure Sucker Used for Cleaning Work

The Wall climbing Robot is a kind of robot used in extremely hazardous environment, which has very widely use. In this paper, we introduced a new kind of wall climbing robot, which are used in cleaning work. It was designed with single sucker and special designed sealing mechanism, which can be used to do cleaning work on ceramic and glasses surface of high buildings(about 70 meters).