Immature embryos, mature embryos and embryogenic calli of 6 rice (Oryza sativa L.) materials were transformed with particle bombardment. The plasmids pSSVstl and pVE5+ were used, both containing the phytoalexin gene f...Immature embryos, mature embryos and embryogenic calli of 6 rice (Oryza sativa L.) materials were transformed with particle bombardment. The plasmids pSSVstl and pVE5+ were used, both containing the phytoalexin gene from grapevine coding for stilbene synthase, but driven by 35S and its own promoter respectively. Through resistance selection for G418 (100 to 150 mg/L) or hygromycin (50 mg/L), 54 independent transgenic plants were isolated and further assessed by PCR, Southern blot and Dot blot analyses. The transgenic plants and their progenies were tested for resistance to blast ( Pyricularia oryzae ) and bacterial blight of rice ( Xanthomonas oryzae ). Preliminary results indicated that the stilbene synthase gene could enhance the resistance of transgenic plants and their progenies to both pathogens.展开更多
Resveratrol is a kind of important phenolic compound in grape, and plays an important role in resisting human tumor, controlling blood glucose, preventing cardiovascular disease, resisting inflammation and protecting ...Resveratrol is a kind of important phenolic compound in grape, and plays an important role in resisting human tumor, controlling blood glucose, preventing cardiovascular disease, resisting inflammation and protecting the nervous system, etc. The main factors affecting the synthesis of resveratrol include internal factors and external factors. The internal factors are genetic control, grape varieties and development stages, and the external factors include ultraviolet radiation, salicylic acid, methyl jasmonate, polysaccharides or oligosaccharides, etc. Under natural condition, there is very little resveratrol in grape, and in order to improve the amount of synthesized resveratrol in grape, induction with external factors is necessary. Therefore, the study of influence factors on resveratrol synthesis in grape growth and development process is of great significance.展开更多
文摘Immature embryos, mature embryos and embryogenic calli of 6 rice (Oryza sativa L.) materials were transformed with particle bombardment. The plasmids pSSVstl and pVE5+ were used, both containing the phytoalexin gene from grapevine coding for stilbene synthase, but driven by 35S and its own promoter respectively. Through resistance selection for G418 (100 to 150 mg/L) or hygromycin (50 mg/L), 54 independent transgenic plants were isolated and further assessed by PCR, Southern blot and Dot blot analyses. The transgenic plants and their progenies were tested for resistance to blast ( Pyricularia oryzae ) and bacterial blight of rice ( Xanthomonas oryzae ). Preliminary results indicated that the stilbene synthase gene could enhance the resistance of transgenic plants and their progenies to both pathogens.
文摘Resveratrol is a kind of important phenolic compound in grape, and plays an important role in resisting human tumor, controlling blood glucose, preventing cardiovascular disease, resisting inflammation and protecting the nervous system, etc. The main factors affecting the synthesis of resveratrol include internal factors and external factors. The internal factors are genetic control, grape varieties and development stages, and the external factors include ultraviolet radiation, salicylic acid, methyl jasmonate, polysaccharides or oligosaccharides, etc. Under natural condition, there is very little resveratrol in grape, and in order to improve the amount of synthesized resveratrol in grape, induction with external factors is necessary. Therefore, the study of influence factors on resveratrol synthesis in grape growth and development process is of great significance.
