Effect of electronic stimulation at Neiguan(PC 6) acupoint on gene expression of adenosine triphosphate-sensitive potassium channel and protein kinases in rats with myocardial ischemia

OBJECTIVE:To investigate the effects of electronic stimulation at acupoints Neiguan(PC 6) and Lieque(LU 7) on the gene expression of the adenosine triphosphate(ATP)-Sensitive potassium channel(KATP:Kir6.1,Kir6.2,SUR2 A,and SUR2B) and protein kinases(PKA,PKG,and PKCβ2) in myocardial cells of rats with myocardial ischemia(Ml) induced by isoproterenol(ISO).METHODS:Rats were randomly divided into a control,model,Neiguan(PC 6),Lieque(LU 7),and non-acupoint groups.The Ml model was established by injecting rats with ISO.Electro-acupuncture treatment was given to the acupuncture groups,once a day for 7 days.Gene expression was analyzed with real-time PCR.RESULTS:The gene expression of KATP and protein kinases in the model group was higher than those in the control group(P < 0.05).After acupuncture treatment,the KATP and protein kinase expression levels were significantly lower in the Neiguan(PC 6)and Lieque(LU 7) groups compared with the model group[P < 0.05).The Neiguan(PC 6) group lowered these levels significantly more than that of the Lieque(LU 7) group(P < 0.05).No significant differences were observed between the model and non-acupoint groups(P > 0.05).CONCLUSION:Our findings suggest that electronic needling of Neiguan(PC 6) can both reduce the gene expression of KATP and protein kinases in rats with ISO-induced Ml.

Analysis of Non-WSSUS Fading Dispersive Channel Model

The Wide-Sense Stationary Uncorrelated Scattering （WSSUS） model has long been viewed as a basic channel model to describe the fading dispersive channel. But non- WSSUS models have more universal applicability when the wireless mobile channel with broadband is studied with finer and more detailed knowledge of propagation environments. So the four-Dimension （4-D） characteristics of channel, namely time, lag, frequency, and Doppler, should be studied together. In this paper, Wigner-Ville distribution of Time-Frequency （TF） domain is introduced to analyze channel in which the incidence rays are non-stationary and correlated with each other. Several channel models, according to different move modes of incidence rays, with time-varying Doppler shift are designed and 4-D Local Scattering Function （LSF） are computed and simulated respectively. Our simulation results show the LSF present asymmetric and non-periodic TF distri- bution for some symmetric and periodic move modes of incidence rays.