In order to improve the breeding effect of livestock, the data were read from an Excel file with Active Server Page （ASP） programs, and the breeding values of breeding stock were calculated by best linear unbiased p...In order to improve the breeding effect of livestock, the data were read from an Excel file with Active Server Page （ASP） programs, and the breeding values of breeding stock were calculated by best linear unbiased prediction （BLUP） method.展开更多
Lamprotula leai is one of the most commercially important freshwater pearl mussels in China, but there is limited data on its genetic diversity and population structure. In the present study, 119 individuals from four...Lamprotula leai is one of the most commercially important freshwater pearl mussels in China, but there is limited data on its genetic diversity and population structure. In the present study, 119 individuals from four major geographical populations were investigated using 15 microsatellite loci identified via cross-species amplification. A total of 114 alleles were detected, with an average of 7.6 alleles per locus（range： 2 to 21）. Among the four stocks, those from Hung-tse Lake and Poyang Lake had the lowest（0.412） and highest（0.455） observed heterozygosity respectively. The polymorphism information content（PIC） ranged from 0.374 to 0.927（mean： 0.907）. AMOVA showed that 12.56% and 44.68% genetic variances were among populations and within individuals, respectively. Pairwise Fst ranged from 0.073 to 0.146, indicating medium genetic differentiation among the populations. In aggregate, our results suggest that inbreeding is a crucial factor accounting for deviations from Hardy–Weinberg equilibrium at 12 loci. Moreover, the genetic distance among four stocks ranged from 0.192 to 0.890. Poyang Lake and Hung-tse Lake were clustered together, joined with Dongting Lake and Anqing Lake. Given that specimens from Hungtse Lake showed the highest average allele richness, expected heterozygosity and PIC, this location may be the source of the highest quality germplasm resources and the stock from this area may be the best for future breeding efforts.展开更多
文摘In order to improve the breeding effect of livestock, the data were read from an Excel file with Active Server Page （ASP） programs, and the breeding values of breeding stock were calculated by best linear unbiased prediction （BLUP） method.
基金Public Welfare Projects of Technology Office in Zhejiang Province （2011C32SA700049） Major Science and Technology Specific Projects of Zhejiang Province （2012C12907-9） Science and Technology Plan Projects of Jinhua City （2011A22020）ACKNOWLEDGEMENTS We would like to thank the Weiwang Pearl Cultivation Base for providing samples. We are also grateful to Professor Yongjiu Chen （Zhejiang Ocean University） for assistance with the manuscript.
文摘Lamprotula leai is one of the most commercially important freshwater pearl mussels in China, but there is limited data on its genetic diversity and population structure. In the present study, 119 individuals from four major geographical populations were investigated using 15 microsatellite loci identified via cross-species amplification. A total of 114 alleles were detected, with an average of 7.6 alleles per locus（range： 2 to 21）. Among the four stocks, those from Hung-tse Lake and Poyang Lake had the lowest（0.412） and highest（0.455） observed heterozygosity respectively. The polymorphism information content（PIC） ranged from 0.374 to 0.927（mean： 0.907）. AMOVA showed that 12.56% and 44.68% genetic variances were among populations and within individuals, respectively. Pairwise Fst ranged from 0.073 to 0.146, indicating medium genetic differentiation among the populations. In aggregate, our results suggest that inbreeding is a crucial factor accounting for deviations from Hardy–Weinberg equilibrium at 12 loci. Moreover, the genetic distance among four stocks ranged from 0.192 to 0.890. Poyang Lake and Hung-tse Lake were clustered together, joined with Dongting Lake and Anqing Lake. Given that specimens from Hungtse Lake showed the highest average allele richness, expected heterozygosity and PIC, this location may be the source of the highest quality germplasm resources and the stock from this area may be the best for future breeding efforts.
