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Vegetative Storage Proteins in Meliaceae 被引量:7
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作者 田维敏 吴继林 +1 位作者 郝秉中 胡正海 《Acta Botanica Sinica》 CSCD 2002年第2期242-245,共4页
采用光学和电子显微镜技术及SDS_PAGE研究了营养贮藏蛋白质 (VSPs)在楝科树木中的分布和超微结构 ;并采用免疫印迹技术 ,以大叶桃花心木 (SwieteniamacrophyllaKing)的 2 1kDVSP的抗血清检测楝科树木VSPs的免疫相关性。结果表明 ,在桃... 采用光学和电子显微镜技术及SDS_PAGE研究了营养贮藏蛋白质 (VSPs)在楝科树木中的分布和超微结构 ;并采用免疫印迹技术 ,以大叶桃花心木 (SwieteniamacrophyllaKing)的 2 1kDVSP的抗血清检测楝科树木VSPs的免疫相关性。结果表明 ,在桃花心木亚科 (Swietenioideae)的树木中普遍存在VSPs ,但楝亚科 (Melioidae)仅部分属的树木有VSPs而椿亚科 (Cedreloideae)树木没有VSPs。VSPs在同一属树木中的分布是一致的。桃花心木亚科和楝亚科树木之间的VSPs几乎没有免疫相关性 ,但是在桃花心木亚科中 ,VSPs具有相对高的同源性。楝科树木VSPs的超微结构存在一定程度的差异 ,这可能与VSPs的种类不同有关。VSPs的分布。 展开更多
关键词 vegetative storage proteins ULTRASTRUCTURE immuno-correlation MELIACEAE
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Rice Storage Proteins:Focus on Composition,Distribution,Genetic Improvement and Effects on Rice Quality 被引量:4
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作者 LONG Xinkang GUAN Chunmin +5 位作者 WANG Lin JIA Liting FU Xiangjin LIN Qinlu HUANG Zhengyu LIU Chun 《Rice science》 SCIE CSCD 2023年第3期207-221,I0035-I0037,共18页
Rice storage proteins(RSPs)are plant proteins with high nutritional quality.As the second largest type of storage substance in rice,it is the main source of protein intake for people who consume rice as a staple food.... Rice storage proteins(RSPs)are plant proteins with high nutritional quality.As the second largest type of storage substance in rice,it is the main source of protein intake for people who consume rice as a staple food.The content and type of RSPs affect the appearance,processing quality and eating quality of rice.These effects involve the distribution of RSPs in rice grains as well as the interactions of RSPs with other components such as starch in rice grains.In the past two decades,some progress has been made in the genetic improvement of RSPs.However,the determination mechanism of protein content and composition in rice is still unclear,and the mechanism of the effect of RSPs on rice quality has not been elucidated.In this review,the composition,biosynthesis and distribution of RSPs,and quantitative trait loci mapping and cloning of RSP genes are summarized,the research progress of the influence of RSPs and their components on rice quality are reviewed,and the research directions in the future are proposed. 展开更多
关键词 rice storage protein COMPOSITION DISTRIBUTION genetic improvement rice quality
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Genetic Diversity of Seed Storage Proteins in Different Ecotype Varieties of japonica Rice and Its Application 被引量:3
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作者 JIN Wei-dong LI Na HONG De-lin 《Rice science》 SCIE 2006年第2期85-92,共8页
One hundred and fifteen varieties (including cultivars and lines) with different ecotypes in japonica rice (Oryza sativa L.) were analyzed for endosperm storage proteins by sodium dodecyl sulfate polyacrylamide ge... One hundred and fifteen varieties (including cultivars and lines) with different ecotypes in japonica rice (Oryza sativa L.) were analyzed for endosperm storage proteins by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) to estimate their genetic diversity for the purpose of genetic improving and variety identification. Nineteen types of profile were identified according to 1) presence/absence of 65 kDa bands, 2) staining intensity of 70, 60, 57, 37-39, 22-23, 13 and 10 kDa bands, 3) migration velocity of 35 kDa (α-4) and 4) band number at 57 kDa location. An unweighted-pair group average method with arithmetic mean (UPGMA) dendregram based on the cluster analysis of genetic similarity of the protein bands showed a small genetic variation among the tested materials, with the similarity coefficients varying between 0.75 and 1.00. Three distinct groups were identified from the cluster analysis of the rice varieties studied at the similarity coefficient level of 0.894. The first group included eight varieties with high amylose content, the second group contained fifteen varieties with high protein content, and the third group had the remaining ninety-two varieties, which accounted for 80% of the total materials. Clear relationship between ecotypes distinguished by maturity and groups revealed by cluster analysis was not found in this study. Only the group of high amylose linked with medium-maturity medium japonica ecotype. The bands of 70 kDa and 65 kDa can be used as protein markers to identify F1 seed purity of japonica hybrid rice Liuyanyou 422. 展开更多
关键词 genetic diversity japonica rice ECOTYPE storage protein ELECTROPHORESIS
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Rice seed storage proteins:Biosynthetic pathways and the effects of environmental factors 被引量:10
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作者 Wei He Long Wang +1 位作者 Qinlu Lin Feng Yu 《Journal of Integrative Plant Biology》 SCIE CAS CSCD 2021年第12期1999-2019,共21页
Rice(Oryza sativa L.)is the most important food crop for at least half of the world’s population.Due to improved living standards,the cultivation of high-quality rice for different purposes and markets has become a m... Rice(Oryza sativa L.)is the most important food crop for at least half of the world’s population.Due to improved living standards,the cultivation of high-quality rice for different purposes and markets has become a major goal.Rice quality is determined by the presence of many nutritional components,including seed storage proteins(SSPs),which are the second most abundant nutrient components of rice grains after starch.Rice SSP biosynthesis requires the participation of multiple organelles and is influenced by the external environment,making it challenging to understand the molecular details of SSP biosynthesis and improve rice protein quality.In this review,we highlight the current knowledge of rice SSP biosynthesis,including a detailed description of the key molecules involved in rice SSP biosynthetic processes and the major environmental factors affecting SSP biosynthesis.The effects of these factors on SSP accumulation and their contribution to rice quality are also discussed based on recent findings.This recent knowledge suggests not only new research directions for exploring rice SSP biosynthesis but also innovative strategies for breeding high-quality rice varieties. 展开更多
关键词 GLOBULIN GLUTELIN high temperature nitrogen fertilizer prolamine RICE seed storage proteins
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Fluctuation of Vegetative Storage Proteins in the Seedlings of Swietenia macrophylla, Analogous to the Seasonal Changes of Those in the Shoot of the Adult Tree 被引量:2
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作者 Ya-Qin Han Xing-Fu Yan +3 位作者 Hua Zhang Zheng-Hai Hu Bing-Zhong Hao Wei-Min Tian 《Journal of Integrative Plant Biology》 SCIE CAS CSCD 2007年第3期362-367,共6页
In order to Identify appropriate plant materials for studying the gene expression and biological function of vegetative storage proteins (VSPs) in woody plants, the VSPs in the seedlings of Swietenla rnacrophylla Ki... In order to Identify appropriate plant materials for studying the gene expression and biological function of vegetative storage proteins (VSPs) in woody plants, the VSPs in the seedlings of Swietenla rnacrophylla King were investigated by using light microscopy, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western.blotting. The seed of S. macrophylla was rich in storage proteins that accumulated In the vacuoles of cotyledon parenchyma cells in appearance of compact spherical grains. The growth and development of S. macrophylla seedlings were characterized by an obvious growth rhythm. The storage proteins In seeds disappeared during seedling growth while VSPs appeared in the stem 2 weeks after seedling leaves matured. Thereafter, the VSPs In the seedling stem almost exhausted during new shoot growth, and when the leaves of new shoot Just matured, both the stem beneath the new shoot of seedlings and the stem of new shoot started to accumulate VSPs. Nitrogen application dramatically Increased the level of VSPs, but had little influence on the dynamics of VSP consumption and accumulation in seedling stem. Together with these data, the fluctuation of VSPs in seedlings was very similar to that in the branches of the adult trees. In addition, seedlings are easy to be treated due to their small size. Our results suggested that S. rnacrophylla seedlings were suitable for Investigating the biological roles of VSPs and the mechanism of nitrogen storage in trees. 展开更多
关键词 rhythmical growth SEEDLINGS Swietenia macrophylla King vegetative storage proteins
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Identification in Lupin Seed of a Serine- Endopeptidase Activity Cleaving between Twin Arginine Pairs and Causing Limited Proteolysis of Seed Storage Proteins
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作者 Chiara Magni Fabio Sessa +4 位作者 Gabriella Tedeschi Armando Negri Alessio Scarafoni Alessandro Consonni Marcello Duranti 《Molecular Plant》 SCIE CAS CSCD 2012年第5期1011-1019,共9页
The occurrence of twin-arginine motifs (-R-R-) in the amino acid sequences of animal pro-proteins frequently defines the cleavage site(s) for their structural/functional maturation. No information is available on ... The occurrence of twin-arginine motifs (-R-R-) in the amino acid sequences of animal pro-proteins frequently defines the cleavage site(s) for their structural/functional maturation. No information is available on the presence and possible biological meaning of these motifs in the seed storage proteins. In this work, a novel endopeptidase activity with cleavage specificity to twin-arginine pairs has been detected in mature dry Lupinus albus seeds. The endopeptidase was tested with a number of endogenous and exogenous protein substrates, which were selected according to the pres- ence of one or more twin-arginine residue motifs in their amino acid sequences. The observed hydrolysis patterns were limited and highly specific. Partial proteolysis led to stable polypeptide fragments that were characterized by 1- and 2-D electrophoresis. Selected polypeptides were submitted to N-terminal amino acid sequencing and mass spectrometry anal- yses, These approaches, supported by bioinformatic analysis of the available sequences, allowed the conclusion that the polypeptide cleavage events had occurred at the peptide bonds comprised between twin-arginine residue pairs with all tested protein substrates. The endopeptidase activity was inhibited by 4-(2-AminoEthyl)Benzene-Sulphonyl Fluoride hy- drochloride (AEBSF), leupeptin, and serine proteinase protein inhibitors, while it was not affected by pepstatin, trans- EpoxysuccinyI-L-leucylamido(4-guanidino)butane (E64), and ethylenediaminetetraacetic acid (EDTA), thus qualifying the Arg-Arg cleaving enzyme as a serine endopeptidase. The structural features of storage proteins from lupin and other legume seeds strongly support the hypothesis that the occurrence of an endopeptidase activity cleaving -R-R- bonds may be functional to facilitate their degradation at germination and possibly generate polypeptide fragments with specific biological activity. 展开更多
关键词 Lupinus albus leguminous seeds twin-arginine residues PROTEOLYSIS storage proteins proteolytic conver-sion N-terminal amino acid sequence analysis.
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Effects of water deficit and high N fertilization on wheat storage protein synthesis,gluten secondary structure,and breadmaking quality 被引量:4
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作者 Junxian Liu Junwei Zhang +2 位作者 Gengrui Zhu Dong Zhu Yueming Yan 《The Crop Journal》 SCIE CSCD 2022年第1期216-223,共8页
The content and composition of wheat storage proteins are the major determinants of dough rheological properties and breadmaking quality and are influenced by cultivation conditions.This study aimed to investigate the... The content and composition of wheat storage proteins are the major determinants of dough rheological properties and breadmaking quality and are influenced by cultivation conditions.This study aimed to investigate the effects of water deficit and high N-fertilizer application on wheat storage protein synthesis,gluten secondary structure,and breadmaking quality.Reverse-phase ultrahigh-performance liquid chromatography analysis showed that storage protein and gluten macropolymer accumulation was promoted under both independent applications and a combination of water-deficit and high N-fertilizer treatments.Fourier-transform infrared spectroscopy showed that water deficit and high N-fertilizer treatments generally improved protein secondary structure formation and lipid accumulation,and reduced flour moisture.In particular,high N-fertilizer application increasedβ-sheet content by 10.4%and the combination of water-deficit and high N-fertilizer treatments increased random coil content by 7.6%.These changes in gluten content and secondary structure led to improved dough rheological properties and breadmaking quality,including superior loaf internal structure,volume,and score.Our results demonstrate that moderately high N-fertilizer application under drought conditions can improve gluten accumulation,gluten secondary structure formation,and baking quality. 展开更多
关键词 Water deficit High N-fertilizer storage proteins Gluten structure Breadmaking quality
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A Dominant Locus, qBSC-1, Controls β Subunit Content of Seed Storage Protein in Soybean(Glycine max(L.) Merri.) 被引量:5
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作者 WANG Jun LIU Lin +8 位作者 GUO Yong WANG Yong-hui ZHANG Le JIN Long-guo GUAN Rong-xia LIU Zhang-xiong WANG Lin-lin CHANG Ru-zhen QIU Li-juan 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2014年第9期1854-1864,共11页
Soybean seed storage protein is one of the most important plant vegetable proteins, and β subunit is of great significance to enhance soybean protein quality and processing property. F2 segregated population and resi... Soybean seed storage protein is one of the most important plant vegetable proteins, and β subunit is of great significance to enhance soybean protein quality and processing property. F2 segregated population and residual heterozygous lines(RHL) derived from the cross between Yangyandou(low level of β subunit) and Zhonghuang 13(normal level of β subunit) were used for mapping of β subunit content. Our results showed that β subunit content was controlled by a single dominant locus, qBSC-1(β subunit content), which was mapped to a region of 11.9 cM on chromosome 20 in F2 population of 85 individuals. This region was narrowed down to 2.5 cM between BARCSOYSSR_20_0997 and BARCSOYSSR_20_0910 in RHL with a larger population size of 246 individuals. There were 48 predicted genes within qBSC-1 region based on the reference genome(Glyma 1.0, Williams 82), including the two copies of β subunit coding gene CG4. An InDel marker developed from a thymine(TT) insertion in one copy of CG4 promoter region in Yangyandou cosegregrated with BARCSOYSSR_20_0975 within qBSC-1 region, suggesting that this InDel marker maybe useful for marker-assisted selection(MAS). 展开更多
关键词 soybean seed storage protein β subunit QTL mapping
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Characterization of Agro-diversity by Seed Storage Protein Electrophoresis:Focus on Rice Germplasm from Uttarakhand Himalaya,India 被引量:2
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作者 Arun JUGRAN Indra D.BHATT Ranbeer S.RAWAL 《Rice science》 SCIE 2010年第2期122-128,共7页
The characteristics of 48 rice varieties from Uttarakhand Himalaya, India were detected by morphological and biochemical markers. The grains of the selected rice varieties varied in their morphological (grain length,... The characteristics of 48 rice varieties from Uttarakhand Himalaya, India were detected by morphological and biochemical markers. The grains of the selected rice varieties varied in their morphological (grain length, grain width and grain weight) and biochemical characters (sodium dodecyl sulfate polyacrylamide gel electrophoresis, SDS-PAGE). Based on the presence of 70, 65, 60, 57, 37-39, 22-23, 13 and 10 kDa protein bands in the 48 rice varieties, seven types of profiles were identified. An unweighted pair group average method with arithmetic mean (UPGMA) dendrogram based on cluster analysis of genetic similarity of the protein bands showed two distinct groups with 1%-78% similarity coefficients. The presence of characteristic bands in selected varieties is a useful parameter for identification of rice germplasm. 展开更多
关键词 rice seed storage protein sodium dodecyl sulfate polyacrylamide gel electrophoresis genetic diversity POLYMORPHISM varietal identification
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New combination in Veronica (Scrophulariaceae s.l.) based on morphological characters and the seed storage protein polymorphism
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作者 Cem VURAL Servet ZCAN Mikail AKBULUT 《Journal of Systematics and Evolution》 SCIE CSCD 北大核心 2009年第2期168-172,共5页
Veronica erciyasdagi (M. A. Fischer) C. Vural comb. & stat. nov., previously regarded as a variety, is proposed as a new combination, based on the morphological characters and seed storage protein polymorphism pres... Veronica erciyasdagi (M. A. Fischer) C. Vural comb. & stat. nov., previously regarded as a variety, is proposed as a new combination, based on the morphological characters and seed storage protein polymorphism presented in this study. In addition, information about the ecology and conservation status of Veronica erciyasdagi was reported. This taxon is endemic to central Anatolia, Turkey and is critically endangered. 展开更多
关键词 critically endangered plant MORPHOLOGY SCROPHULARIACEAE seed storage protein polymorphism Veronica erciyasdagi.
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Processing Pisum sativum seed storage protein precursors in vitro
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作者 YANGLIJUN CDOMONEY 《Cell Research》 SCIE CAS CSCD 1990年第2期153-162,共10页
The profile of polypeptides separated by SDS-PAGE from seed of major crop species such as pea (Pisum sativum) is complex, resulting from cleavage (processing) of precursors expressed from multiple copies of genes enco... The profile of polypeptides separated by SDS-PAGE from seed of major crop species such as pea (Pisum sativum) is complex, resulting from cleavage (processing) of precursors expressed from multiple copies of genes encoding vicilin and legumin, the major storage globulins. Translation in vitro of mRNAs hybrid-selected from mid-maturation pea seed RNAs by denned vicilin and legumin cDNA clones provided precursor molecules that were cleaved in vitro by a cell-free protease extract obtained from similar stage seed; the derived polypep tides were of comparable sizes to those observed in vivo. The feasibility of transcribing mENA in vitro from a cDNA clone and cleavage in vitro of the derived translation products was established for a legumin clone, providing a method for determining polypeptide products of an expressed sequence. This approach will also be useful for characterising cleavage site requirements since modifications an readily be introduced at the DNA level. 展开更多
关键词 LEGUMIN PISUM PROCESSING storage protein precursors Vicilin.
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Isolation and Characterization of Storage Protein Subunit-null-dwarf Mutants in Soybean(Glycine max(L.) Merr.)
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作者 Luo Ting-ting Song Ying-ji +7 位作者 Pang Ze Liu Han-miao Waqar Ahmed Khuhro Li Ming-xue Qiu Zhen-dong Wei Xiao-shuang Song Bo Liu Shan-shan 《Journal of Northeast Agricultural University(English Edition)》 CAS 2019年第4期11-22,共12页
Dwarfing is useful to reduce plant height,when breeding high-yielding and non-lodging crops.In this study,a set of natural storage protein subunit-null dwarf mutants of soybean was reported that showed strongly reduce... Dwarfing is useful to reduce plant height,when breeding high-yielding and non-lodging crops.In this study,a set of natural storage protein subunit-null dwarf mutants of soybean was reported that showed strongly reduced plant stature and deficiency in various 7S and 11S subunits,designated as snd1 mutants.Under normal growth conditions,the snd1 mutants showed a severe dwarf phenotype,with plant height of about 25 cm.Compared with wild-type DN47,the mutant snd1 exhibited no obvious morphological differences at the early stage of development.All the snd1 mutants examined had fewer nodes and shorter than normal internodes;the leaves were similar in shape to normal parents,but were dark-green at the mature stage.The flower size was similar to DN47;however,the flowering period was shorter than in the wild-type.Significant variation was noted for protein content,oil content of the seeds and size of seeds(weight of 100 seeds)among 17 snd1 dwarf lines.Genetic analysis indicated that the dwarfism of snd1 was controlled by a single recessive gene.The snd1 dwarf mutant had markedly different dynamic levels of the endogenous hormones gibberellin(GA),brassinosteroid,indole-3-acetic acid and abscisic acid,at the seedling stage.Exogenous GA3 treatment led to recovery of the plant height phenotype of the snd1 mutant;GA3 at 0.1 mm had the largest effect on enhancing plant height.Using molecular markers,snd1 gene was approximately mapped in an interval of 603 kb between markers Satt166 and Satt561 on chromosome 19.Snd1 mutant provided valuable material for hypoallergenic soybean breeding and the snd1 gene might be a novel gene related to plant height in soybean. 展开更多
关键词 soybean(Glycine max) storage protein subunit-null dwarf mutant genetic analysis gibberellic acid mapping
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Influence of protein characteristics and the proportion of gluten on end-use quality in Korean wheat cultivars 被引量:7
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作者 Seong-Woo Cho Chon-Sik Kang +3 位作者 Hyeon Seok Ko Byung-Kee Baik Kwang-Min Cho Chul Soo Park 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2018年第8期1706-1719,共14页
The effects of protein characteristics and the proportion of gluten on end-use quality in 13 Korean wheat cultivars for three years were verified in this study. Year, cultivar, and the interaction between the year and... The effects of protein characteristics and the proportion of gluten on end-use quality in 13 Korean wheat cultivars for three years were verified in this study. Year, cultivar, and the interaction between the year and the cultivar influenced protein characteristics, the proportion of gluten except for γ-and ω-gliadin using RP-HPLC(reversed-phase high-performance liquid chromatography), and end-use quality. Protein characteristics and the proportion of gluten in Korean wheat cultivars were between those of Australian standard white(ASW) and hard wheat(AH). Korean wheat cultivars exhibited a higher average α+β gliadin proportion than imported wheat, a γ-gliadin proportion similar to that of dark northern spring wheat, and the same ω-gliadin proportion as AH. They showed a bread-loaf volume intermediate between those of ASW and AH and a texture of cooked noodles similar to that of soft white wheat, but less springiness than imported wheat. The cookie diameter of Korean wheat cultivars was similar to that of hard red winter wheat. There was a correlation between bread-loaf volume and protein characteristics, except for the protein content in Korean wheat cultivars. Springiness and cohesiveness of cooked noodles were not correlated with protein characteristics, while hardness was correlated with the protein content and water absorption of a mixograph. Cookie diameter was negatively correlated with the sodium dodecyl sulfate(SDS) sedimentation volume and water absorption of a mixograph. The end-use quality was not correlated with any proportion of gluten composition. Principal component analysis(PCA) showed that the proportion of gluten was not related to the quality of the bread(both PCs, 81.3%), noodle(77.7%), and cookie(82.4%). PCA explained that Keumkang is suitable for superior bread, while Uri is good for cooked noodles and cookies. 展开更多
关键词 wheat quality flour quality storage proteins
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Molecular identification of ω-secalin gene expression activity in a wheat 1B/1R translocation cultivar 被引量:3
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作者 CHAI Jian-fang ZHANG Cui-mian +1 位作者 MA Xiu-ying WANG Hai-bo 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2016年第12期2712-2718,共7页
ω-Secalin was an important factor influencing processing quality of wheat 1BL/1RS translocations. On the basis of four ω-secalin gene sequences cloned from Lankao 906 (a wheat cultivar with 1BL/1RS translocation) ... ω-Secalin was an important factor influencing processing quality of wheat 1BL/1RS translocations. On the basis of four ω-secalin gene sequences cloned from Lankao 906 (a wheat cultivar with 1BL/1RS translocation) with putative transcription activity, a pair of primers with suitable restriction endonucleases added at their 5" ends were designed to amplify the mature protein-coding regions of the four genes. After digestion with restriction endonucleases, the amplified products were ligated into the prokaryotic expression vector pET30a(+). The prokaryotically expressed recombinant proteins and gliadin isolated from the Lankao 906 seed were analyzed on the same acid polyacrylamide gel electrophoresis. All four prokaryotically expressed secalin bands had corresponding seed-expressed gliadin bands. The four corresponding gliadin bands were confirmed to be the expression products of the four ω-secalin genes by liquid chromatography tandem mass spectrometry (LC-MS/MS). This conclusion was further confirmed because the four ω-secalin bands could be detected in all 14 wheat 1BL/1 RS translocation cultivars used in the study, although there was some interference for the detection of one ω-secalin band from nearby wheat gliadin bands. The sequence information of ω-secalin genes with expression activity will be helpful for improving the processing quality of wheat with 1BL/1RS translocations by using RNA interference method to silence the expression of the ω-secalin genes. 展开更多
关键词 Triticum aestivum processing quality storage proteins prokaryotic expression LC-MS/MS
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Diapause Regulation in the Moth Sesamia nonagrioides (Lepidoptera: Noctuidae)
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作者 Anna Kourti 《Journal of Agricultural Science and Technology(A)》 2013年第12期935-947,共13页
Insects enter in diapause in response to diverse environmental cues. During diapause, insects arrest their development and many genes are down-regulated while a small number of genes uniquely expressed at this time. T... Insects enter in diapause in response to diverse environmental cues. During diapause, insects arrest their development and many genes are down-regulated while a small number of genes uniquely expressed at this time. This review aims to present available data regarding the regulation of diapause in the moth Sesamia nonagrioides (Lepidoptera: Noctuidae). Studying the transcriptional regulation of several genes (five heat shock proteins, two storage proteins and one juvenile hormone esterase) showed that these genes may play various roles in the diapause programming. The results show that SnoHsp19.5 gene was consistently expressed, while SnoHsp20.8 was down-regulated in deep diapause and was up-regulated at the termination of diapause. SnoHsc70 may play important roles in assisting protein conformation during specific stages of diapause. SnoHsp83 displays a similar pattern to SnoHsc70 under diapause conditions, when extra larval moults occur, indicating that could be involved in the developmental process that occurs between two moults. Expression of two SnoSP1 and SnoSP2 hexamerin genes was also observed throughout diapause. And the results lead us to the conclusion that larval diapause of S. nonagrioides is associated with continuous synthesis and accumulation of storage proteins. In addition, the transcript level of the carboxylesterase SnoJHER was higher in non-diapausing larvae than in diapausing ones. During the fifth instar of the non-diapausing and the ninth instar of the diapausing larvae, SnoJHER mRNAs reached higher expression levels on the days close to each larval molt. 展开更多
关键词 Sesamia nonagrioides DIAPAUSE heat shock proteins storage proteins juvenile hormone esterase.
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Antimicrobial Activity of a Cys-Rich Peptide Derived from a Centrosema virginianum Vicilin
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作者 Zhongyu Xie Nibedita Saha Caryl Chlan 《American Journal of Plant Sciences》 2016年第1期92-107,共16页
Many antimicrobial peptides (AMPs) have been identified in plants. These peptides are highly divergent at the primary sequence level and vary in their hierarchical structures. Some common biochemical features include ... Many antimicrobial peptides (AMPs) have been identified in plants. These peptides are highly divergent at the primary sequence level and vary in their hierarchical structures. Some common biochemical features include the ability to form disulfide bonds, tandemly repeated amino acid sequences and a net charge at pH 7. Unusual Cysteine containing repeats has been identified in several plant seed storage proteins that may act as AMPs. We identified a Cys repeat within a vicilin (seed storage protein) of a wild legume, Centrosema virginianum. Cleavage of the vicilin protein during germination would generate a vicilin derived Cys peptide (VDCP). We investigated the antimicrobial properties of this VDCP and compared its efficacy as an antimicrobial agent to VDCPs from other species. We developed transgenic tobacco plants that expressed cloned sequences encoding the Cysteine repeat unit from C. virginianum, Theobroma cacao and Gossypium hirsutum. Extracts from fully expanded leaves were tested for antimicrobial activity against a fungal pathogen, Botrytis cinerea. The Cys motif from C. virginianum was also expressed in two E. coli cell lines (reducing or oxidizing cytoplasm) and peptide fusion protein fractions were tested for antimicrobial activity against a battery of fungal strains. The unique Cysteine repeat single unit from C. virginianum exhibited antimicrobial properties greater than or equal to the antimicrobial activity associated with expression of the multiple Cys-repeat VDCPs from G. hirsutum or T. cacao in transgenic tobacco. When expressed in bacteria, a C. virginianum VDCP fusion protein exhibited antifungal activity against 3 of the 4 fungi tested. Although the primary role of seed storage proteins is to provide a pool of amino acids and nitrogen for germinating seeds and developing plantlets, it is likely that seed storage protein proteolytic products also provide beneficial antimicrobial properties during germination and young plantlet development. 展开更多
关键词 Seed storage proteins Vicilins Antimicrobial Peptides C. virginianum
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Effects of nitrogen fertilizer on protein synthesis, accumulation, and physicochemical properties in common buckwheat 被引量:1
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作者 Chenxi Wan Licheng Gao +4 位作者 Jiale Wang Xinhui Lei Jincai Tao Baili Feng Jinfeng Gao 《The Crop Journal》 SCIE CSCD 2023年第3期941-950,共10页
Nitrogen(N)fertilization affects grain quality in common buckwheat(Fagopyrum esculentum Moench).But the effects of N fertilizer on various buckwheat protein parameters are not fully understood.This study aimed to inve... Nitrogen(N)fertilization affects grain quality in common buckwheat(Fagopyrum esculentum Moench).But the effects of N fertilizer on various buckwheat protein parameters are not fully understood.This study aimed to investigate the synthesis,accumulation,and quality of buckwheat protein under four N application rates in the Loess Plateau,China.Optimal N application(180 kg N ha-1)improved yield,agronomic traits,and N transport and increased protein yield and protein component accumulation.Prolamin and glutelin accumulation first increased and then decreased with increasing N application.The relationships between the contents of protein components and the amount of applied N generally followed quadratic functions.Nitrate reductase and glutamine synthetase activities first increased and then decreased with increasing N levels.Optimal N fertilizer increased the waterholding capacity and thermal stability of buckwheat protein and reduced its emulsification capacity,but negligibly changed its oil-absorption capacity.Hydrophobic amino acids and glutelin content were the main factors affecting protein quality. 展开更多
关键词 Common buckwheat Nitrogen fertilizer storage protein Nitrogen metabolism Grain quality
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Genetic Diversity and Classification of Chinese Elite Foxtail Millet [Setaria italica (L.) P. Beauv.] Revealed by Acid-PAGE Prolamin 被引量:1
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作者 Guoxing Ma Qiang Li +4 位作者 Suying Li Zhengli Liu Yanjiao Cui Jing Zhang Dan Liu 《Agricultural Sciences》 2022年第3期404-428,共25页
Arid and semi-arid regions of China account for more than half of the country. Because of drought resistance and high nutritive value, elite foxtail millet (Setaria Italica (L.) P. Beauv.) is one of the most important... Arid and semi-arid regions of China account for more than half of the country. Because of drought resistance and high nutritive value, elite foxtail millet (Setaria Italica (L.) P. Beauv.) is one of the most important cereal crops in China. Evaluation of germplasm and genetic diversity of foxtail millet is still in its infancy, but prolamin could play an important role as a protein marker. To investigate the genetic diversity and population structure of foxtail millet from different ecological zones of China, 90 accessions of foxtail millet were collected from three major ecological areas: North, Northwest, and Northeast China. The prolamin contents were examined by acid polyacrylamide gel electrophoresis (acid-PAGE). Five to twenty-two prolamin bands appeared in tested varieties, of which were polymorphic, so prolamin patterns of foxtail millet varieties can be used in variety identification and evaluation. Structure analysis identified six groups, which matches their pedigree information but not their geographic origins. This indicated a high degree (87.78%) of consistency with a phylogenetic classification based on SSR. The results showed prolamin banding patterns were an effective method for analyzing foxtail millet genetic variability. 展开更多
关键词 Foxtail Millet [Setaria italica (L.) P. Beauv.] Seed storage Protein Protein Polymorphism PROLAMIN
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Orchestrating seed storage protein and starch accumulation toward overcoming yield–quality trade-off in cereal crops 被引量:1
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作者 Shuanghe Cao Bingyan Liu +4 位作者 Daowen Wang Awais Rasheed Lina Xie Xianchun Xia Zhonghu He 《Journal of Integrative Plant Biology》 SCIE CAS CSCD 2024年第3期468-483,共16页
Achieving high yield and good quality in crops is essential for human food security and health.However,there is usually disharmony between yield and quality.Seed storage protein(SSP)and starch,the predominant componen... Achieving high yield and good quality in crops is essential for human food security and health.However,there is usually disharmony between yield and quality.Seed storage protein(SSP)and starch,the predominant components in cereal grains,determine yield and quality,and their coupled synthesis causes a yield–quality trade-off.Therefore,dissection of the underlying regulatory mechanism facilitates simultaneous improvement of yield and quality.Here,we summarize current findings about the synergistic molecular machinery underpinning SSP and starch synthesis in the leading staple cereal crops,including maize,rice and wheat.We further evaluate the functional conservation and differentiation of key regulators and specify feasible research approaches to identify additional regulators and expand insights.We also present major strategies to leverage resultant information for simultaneous improvement of yield and quality by molecular breeding.Finally,future perspectives on major challenges are proposed. 展开更多
关键词 cereal crops grain yield and quality seed storage protein STARCH synergistic regulation
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Disulphide Linkages Occur in Many Polypeptides of Rice Protein Fractions: a Two-dimensional Gel Electrophoretic Study
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作者 Arvinder SINGH Narender K.MATTA 《Rice science》 SCIE 2011年第2期86-94,共9页
Most of the work on rice storage proteins focused on the major fraction - glutelin. In contrast to previous reports of the occurrence of single legumin-like polypeptide pair in glutelin, two-dimensional gel electropho... Most of the work on rice storage proteins focused on the major fraction - glutelin. In contrast to previous reports of the occurrence of single legumin-like polypeptide pair in glutelin, two-dimensional gel electrophoresis revealed many glutelin bands, with some having and some lacking disulphide-linkages. Five legumin-like polypeptide pairs exhibited wide heterogeneity over a range of molecular weight (Mr) 25 to 60 kDa, each having a large subunit (Mr ranged from 18 to 40.5 kDa) disulphide-bonded to a small subunit (Mr ranged from 16.5 to 18.0 kDa). A band of 49 kDa was homodimeric with two subunits of 29 kDa each; a polypeptide of 51 kDa which altered position to ones corresponding to 53 kDa and 57 kDa on 2-D gels contained intrapolypeptide linkages. Polypeptides of 65 kDa and 60 kDa occurred as aggregates of 110 kDa. The number of polypeptides in other seed protein fractions albumins, globulins and prolamins, varied from four to ten. One of the albumin bands had intrapolypeptide disulphide linkages (20 kDa) and the globulins contained two such bands (13.5 and 20 kDa). Thus, the present study provides a description of the polypeptide composition of different rice protein fractions that is finely resolved with respect to the occurrence of disulphide linkages. 展开更多
关键词 seed storage protein GLUTELIN PROLAMIN GLOBULIN Oryza sativa
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