Enhancing host immunity is an effective way to reduce morbidity in chickens.Heterophil to lymphocyte ratio(H/L)is associated with host disease resistance in birds.Chickens with different H/L levels show different dise...Enhancing host immunity is an effective way to reduce morbidity in chickens.Heterophil to lymphocyte ratio(H/L)is associated with host disease resistance in birds.Chickens with different H/L levels show different disease resistances.However,the utility of the H/L as an indicator of immune function needs to be further analyzed.In this study,a H/L directional breeding chicken line(Jingxing yellow chicken)was constructed,which has been bred for 12 generations.We compared the function of heterophils,and combined statistical analysis to explore the candidate genes and pathways related to H/L.The oxidative burst function of the heterophils isolated from the H/L selection line(G12)was increased(P=0.044)compared to the non-selection line(NS).The 22.44 Mb genomic regions which annotated 300 protein-coding genes were selected in the genome of G9(n=92)compared to NS(n=92)based on a genome-wide selective sweep.Several selective regions were identified containing genes like interferon induced with helicase C domain 1(IFIH1)and moesin(MSN)associated with the intracellular receptor signaling pathway,C–C motif chemokine receptor 6(CCR6),dipeptidyl peptidase 4(DPP4)and hemolytic complement(HC)associated with the negative regulation of leukocyte chemotaxis and tight junction protein 1(TJP1)associated with actin cytoskeleton organization.In addition,45 genome-wide significant indels containing 29 protein-coding genes were also identified as associated with the H/L based on genome-wide association study(GWAS).The expression of protein tyrosine phosphatase non-receptor type 5(PTPN5)(r=0.75,P=0.033)and oxysterol binding protein like 5(OSBPL5)(r=0.89,P=0.0027)were positively correlated with H/L.Compared to the high H/L group,the expressions of PTPN5 and OSBPL5 were decreased(P<0.05)in the low H/L group of Beijing you chicken.The A/A allelic frequency of indel 5_13108985(P=3.85E–06)within OSBPL5 gradually increased from the NS to G5 and G9,and the individuals with A/A exhibited lower H/L than individuals with heterozygote A/ATCT(P=4.28E–04)and homozygous ATCT/ATCT(P=3.40E–05).Above results indicated oxidative burst function of heterophils were enhanced,and 22.44 Mb genomic regions were selected with the directional selection of H/L.In addition,PTPN5 and OSBPL5 genes were identified as H/L-related candidate genes.These findings revealed the complex genetic mechanism of H/L related to immunity and will allow selection for improving chicken immunity based on the H/L.展开更多
The essential role of zonal flow in the L-H transition and the suppression of turbulence have been studied with a long range correlation technique using Langmuir probe arrays in EAST tokamak.Two toroidally localized p...The essential role of zonal flow in the L-H transition and the suppression of turbulence have been studied with a long range correlation technique using Langmuir probe arrays in EAST tokamak.Two toroidally localized probe arrays are used to measure the zonal flow during L-H transition and H-L back transition.The energy ratio of the low frequency zonal flow to the total drift wave turbulence is calculated.During ELM-free H mode,the energy ratio is higher than that in L mode,which reveals the important role of zonal flows in regulating turbulence amplitude in L-H transition.展开更多
基金supported by the grants from the National Natural Science Foundation of China(32072708)the National Key R&D Program of China(2018YFE0128000)the Major Scientific Research Projects of Chinese Academy of Agricultural Sciences(CAAS-ZDRW202005).
文摘Enhancing host immunity is an effective way to reduce morbidity in chickens.Heterophil to lymphocyte ratio(H/L)is associated with host disease resistance in birds.Chickens with different H/L levels show different disease resistances.However,the utility of the H/L as an indicator of immune function needs to be further analyzed.In this study,a H/L directional breeding chicken line(Jingxing yellow chicken)was constructed,which has been bred for 12 generations.We compared the function of heterophils,and combined statistical analysis to explore the candidate genes and pathways related to H/L.The oxidative burst function of the heterophils isolated from the H/L selection line(G12)was increased(P=0.044)compared to the non-selection line(NS).The 22.44 Mb genomic regions which annotated 300 protein-coding genes were selected in the genome of G9(n=92)compared to NS(n=92)based on a genome-wide selective sweep.Several selective regions were identified containing genes like interferon induced with helicase C domain 1(IFIH1)and moesin(MSN)associated with the intracellular receptor signaling pathway,C–C motif chemokine receptor 6(CCR6),dipeptidyl peptidase 4(DPP4)and hemolytic complement(HC)associated with the negative regulation of leukocyte chemotaxis and tight junction protein 1(TJP1)associated with actin cytoskeleton organization.In addition,45 genome-wide significant indels containing 29 protein-coding genes were also identified as associated with the H/L based on genome-wide association study(GWAS).The expression of protein tyrosine phosphatase non-receptor type 5(PTPN5)(r=0.75,P=0.033)and oxysterol binding protein like 5(OSBPL5)(r=0.89,P=0.0027)were positively correlated with H/L.Compared to the high H/L group,the expressions of PTPN5 and OSBPL5 were decreased(P<0.05)in the low H/L group of Beijing you chicken.The A/A allelic frequency of indel 5_13108985(P=3.85E–06)within OSBPL5 gradually increased from the NS to G5 and G9,and the individuals with A/A exhibited lower H/L than individuals with heterozygote A/ATCT(P=4.28E–04)and homozygous ATCT/ATCT(P=3.40E–05).Above results indicated oxidative burst function of heterophils were enhanced,and 22.44 Mb genomic regions were selected with the directional selection of H/L.In addition,PTPN5 and OSBPL5 genes were identified as H/L-related candidate genes.These findings revealed the complex genetic mechanism of H/L related to immunity and will allow selection for improving chicken immunity based on the H/L.
基金the EAST team for their support during the experimentssupported by the National Natural Science Foundation of China with Grant Nos.10990210,10990211,11375188,11105144,and 11375053+1 种基金the National Magnetic Confinement Fusion Science Program of China under Contracts Nos.2013GB106002, 2013GB106003the Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology with Grant No.2014FXCX003
文摘The essential role of zonal flow in the L-H transition and the suppression of turbulence have been studied with a long range correlation technique using Langmuir probe arrays in EAST tokamak.Two toroidally localized probe arrays are used to measure the zonal flow during L-H transition and H-L back transition.The energy ratio of the low frequency zonal flow to the total drift wave turbulence is calculated.During ELM-free H mode,the energy ratio is higher than that in L mode,which reveals the important role of zonal flows in regulating turbulence amplitude in L-H transition.
