Objective:To evaluate the antibacterial activity of Cinnamomum verum(C.verum) from32 different essential oils against cariogenic bacteria,Streptococcus mutans(S.mutans)and Streptococcus sobrinus(S.sobrinus).Methods:Th...Objective:To evaluate the antibacterial activity of Cinnamomum verum(C.verum) from32 different essential oils against cariogenic bacteria,Streptococcus mutans(S.mutans)and Streptococcus sobrinus(S.sobrinus).Methods:The antibacterial activities of each essential oil were individually investigated against S.mutans and S.sobrinus.The essential oil of C.verum was selected for further evaluation against S.mutans and S.sobrinus.Gas chromatography mass spectrometry was used to determine the major constituents of C.verum essential oil.In addition,the minimum inhibitory concentration(MIC) and minimum bactericidal concentration of the most effective constituent was investigated.Results:The essential oil from C.verum exhibited the greatest antibacterial activity.Gas chromatography mass spectrometry analysis revealed that the major components of C.verum essential oil were cinnamaldehyde(56.3%),cinnamyl acetate(7.1%) and bphellandrene(6.3%).The MIC of cinnamaldehyde was measured using broth dilution assays.The MIC of cinnamaldehyde was 0.02%(v/v) against both bacterial strains tested.The minimum bactericidal concentration of cinnamaldehyde against S.mutans and S.sobrinus were 0.2% and 0.1%(v/v),respectively.Conclusions:The essential oil of C.verum and its major component cinnamaldehyde possessed considerable in vitro antibacterial activities against cariogenic bacteria,S.mutans and S.sobrinus strains.These results showed that the essential oil of C.verum and its bioactive component,cinnamaldehyde,have potential for application as natural agents for the prevention and treatment of dental caries.展开更多
Caries is a disease of human dentition characterized by the loss of mineralized surfaces of the tooth;it is an infectious disease of the oral cavity in which biofilms play a causative role. Control of biofilms has tra...Caries is a disease of human dentition characterized by the loss of mineralized surfaces of the tooth;it is an infectious disease of the oral cavity in which biofilms play a causative role. Control of biofilms has traditionally relied on non-specific removal of plaque by mechanical means such as brushing, although it is difficult to remove biofilms by this method. Caries is also a widespread infection in children. Streptococcus mutans and S. sobrinus are important causative agents of caries. They produce a homologous exocellular polysaccharide called glucan, which strongly adheres to the enamel surface. This is a review of oral microbial biofilm formation by S. mutans and other related bacteria.展开更多
目的:建立一种快速、简便地同时检测变形链球菌和远缘链球菌的方法。方法:以变形链球菌Dextranase基因设计引物,用PCR检测变形链球菌群细菌。结果:变形链球菌(血清型c、e、f)的PCR扩增产物为720 bp;远缘链球菌(血清型d、g)的PCR扩增产物...目的:建立一种快速、简便地同时检测变形链球菌和远缘链球菌的方法。方法:以变形链球菌Dextranase基因设计引物,用PCR检测变形链球菌群细菌。结果:变形链球菌(血清型c、e、f)的PCR扩增产物为720 bp;远缘链球菌(血清型d、g)的PCR扩增产物为460 bp;道勒链球菌(血清型h)910 bp;仓鼠链球菌(血清型a),鼠链球菌(血清型b)均为1 270 bp。其它异种菌均不能扩增出产物,因此该PCR检测具有高度的特异性。从细菌纯培养物中PCR检测的敏感性为105菌落形成单位(colony-form ing un its,CFU)。结论:PCR能同时检测变形链球菌和远缘链球菌。该检测方法具有较高的敏感性和特异性,有望运用于临床检测。展开更多
目的研究维吾尔族和汉族不同龋敏感儿童牙菌斑中变异链球菌和远缘链球菌的检出量,分析变异链球菌及远缘链球菌与不同民族儿童乳牙龋齿发生的关系,为不同民族儿童乳牙龋的防治提供依据。方法采用T-A克隆技术制备变异链球菌ATCC25175、远...目的研究维吾尔族和汉族不同龋敏感儿童牙菌斑中变异链球菌和远缘链球菌的检出量,分析变异链球菌及远缘链球菌与不同民族儿童乳牙龋齿发生的关系,为不同民族儿童乳牙龋的防治提供依据。方法采用T-A克隆技术制备变异链球菌ATCC25175、远缘链球菌ATCC6715、内参基因16S rRNA的质粒标准品,应用SYBR Green I荧光定量聚合酶链反应对变异链球菌和远缘链球菌进行定量检测。结果维吾尔族儿童高龋组远缘链球菌的检出量高于汉族儿童高龋组[(1.06×105±0.43×103)copies.μL-1vs(8.29×104±1.20×104)copies.μL-1],差异有统计学意义(P<0.05),而维吾尔族、汉族儿童高龋组变异链球菌检出量比较差异无统计学意义(P>0.05),同时维吾尔族和汉族儿童远缘链球菌和变异链球菌的检出量高龋组均高于无龋组,差异均有统计学意义(P<0.05)。结论远缘链球菌可能是维吾尔族儿童乳牙龋高发的危险因素。展开更多
基金Supported by Basic Science Research Program through the National Research Foundation of Korea funded by the Ministry of Education(2015R1A6A1A03031413)
文摘Objective:To evaluate the antibacterial activity of Cinnamomum verum(C.verum) from32 different essential oils against cariogenic bacteria,Streptococcus mutans(S.mutans)and Streptococcus sobrinus(S.sobrinus).Methods:The antibacterial activities of each essential oil were individually investigated against S.mutans and S.sobrinus.The essential oil of C.verum was selected for further evaluation against S.mutans and S.sobrinus.Gas chromatography mass spectrometry was used to determine the major constituents of C.verum essential oil.In addition,the minimum inhibitory concentration(MIC) and minimum bactericidal concentration of the most effective constituent was investigated.Results:The essential oil from C.verum exhibited the greatest antibacterial activity.Gas chromatography mass spectrometry analysis revealed that the major components of C.verum essential oil were cinnamaldehyde(56.3%),cinnamyl acetate(7.1%) and bphellandrene(6.3%).The MIC of cinnamaldehyde was measured using broth dilution assays.The MIC of cinnamaldehyde was 0.02%(v/v) against both bacterial strains tested.The minimum bactericidal concentration of cinnamaldehyde against S.mutans and S.sobrinus were 0.2% and 0.1%(v/v),respectively.Conclusions:The essential oil of C.verum and its major component cinnamaldehyde possessed considerable in vitro antibacterial activities against cariogenic bacteria,S.mutans and S.sobrinus strains.These results showed that the essential oil of C.verum and its bioactive component,cinnamaldehyde,have potential for application as natural agents for the prevention and treatment of dental caries.
文摘Caries is a disease of human dentition characterized by the loss of mineralized surfaces of the tooth;it is an infectious disease of the oral cavity in which biofilms play a causative role. Control of biofilms has traditionally relied on non-specific removal of plaque by mechanical means such as brushing, although it is difficult to remove biofilms by this method. Caries is also a widespread infection in children. Streptococcus mutans and S. sobrinus are important causative agents of caries. They produce a homologous exocellular polysaccharide called glucan, which strongly adheres to the enamel surface. This is a review of oral microbial biofilm formation by S. mutans and other related bacteria.
文摘目的:建立一种快速、简便地同时检测变形链球菌和远缘链球菌的方法。方法:以变形链球菌Dextranase基因设计引物,用PCR检测变形链球菌群细菌。结果:变形链球菌(血清型c、e、f)的PCR扩增产物为720 bp;远缘链球菌(血清型d、g)的PCR扩增产物为460 bp;道勒链球菌(血清型h)910 bp;仓鼠链球菌(血清型a),鼠链球菌(血清型b)均为1 270 bp。其它异种菌均不能扩增出产物,因此该PCR检测具有高度的特异性。从细菌纯培养物中PCR检测的敏感性为105菌落形成单位(colony-form ing un its,CFU)。结论:PCR能同时检测变形链球菌和远缘链球菌。该检测方法具有较高的敏感性和特异性,有望运用于临床检测。
文摘目的研究维吾尔族和汉族不同龋敏感儿童牙菌斑中变异链球菌和远缘链球菌的检出量,分析变异链球菌及远缘链球菌与不同民族儿童乳牙龋齿发生的关系,为不同民族儿童乳牙龋的防治提供依据。方法采用T-A克隆技术制备变异链球菌ATCC25175、远缘链球菌ATCC6715、内参基因16S rRNA的质粒标准品,应用SYBR Green I荧光定量聚合酶链反应对变异链球菌和远缘链球菌进行定量检测。结果维吾尔族儿童高龋组远缘链球菌的检出量高于汉族儿童高龋组[(1.06×105±0.43×103)copies.μL-1vs(8.29×104±1.20×104)copies.μL-1],差异有统计学意义(P<0.05),而维吾尔族、汉族儿童高龋组变异链球菌检出量比较差异无统计学意义(P>0.05),同时维吾尔族和汉族儿童远缘链球菌和变异链球菌的检出量高龋组均高于无龋组,差异均有统计学意义(P<0.05)。结论远缘链球菌可能是维吾尔族儿童乳牙龋高发的危险因素。
