Detecting protein expression levels in organisms exposed to environmental pollutants can help us understand the action mechanism of toxicity. In the present study, adult female Sprague-Dawley (SD) rats received a cons...Detecting protein expression levels in organisms exposed to environmental pollutants can help us understand the action mechanism of toxicity. In the present study, adult female Sprague-Dawley (SD) rats received a consecutive intraperitoneal injection of tris(2,3-dibromopropyl) isocyanurate (TBC) for seven days, and the ^16O/^18O labeling comparative proteomic approach was used to study the change of liver proteome in these rats. Thirty six differential proteins were identified between the control group and the high-dose-exposed group (8 mg/kg), of which, twenty eight proteins were down-regulated and eight proteins were up-regulated. Bioinformatics analysis revealed that most of the differential proteins were related with the metabolic and cellular processes. In addition, the contents of malondialdehyde (MDA), catalase (CAT), and glutathione (GSH) in liver were measured to evaluate the oxidative stress status induced by TBC exposure. Together, these findings showed that TBC might be toxic to liver by disrupting metabolic process and apoptosis. The results might provide a better insight into the mechanism of toxicity induced by TBC.展开更多
为明确不同生育期干旱胁迫与氮肥施用对花生氮素吸收利用的影响,利用^(15)N示踪技术,研究了不同水分条件下氮肥施用对花生各器官肥料氮吸收利用以及氮肥残留和损失情况的影响。水分设置为正常供水(WW,75%~80%田间持水量)、花针期轻度干...为明确不同生育期干旱胁迫与氮肥施用对花生氮素吸收利用的影响,利用^(15)N示踪技术,研究了不同水分条件下氮肥施用对花生各器官肥料氮吸收利用以及氮肥残留和损失情况的影响。水分设置为正常供水(WW,75%~80%田间持水量)、花针期轻度干旱胁迫(FD,55%~60%田间持水量)和结荚期轻度干旱胁迫(PD,55%~60%田间持水量)3个条件,氮肥水平设置为不施氮(LN)、中氮(MN,90 kg hm^(-2))、高氮(HN,180 kg hm^(-2))。结果表明,与正常供水条件相比,不同生育期干旱胁迫均降低了花生产量和植株氮素积累量,且花针期干旱胁迫的降低幅度大于结荚期干旱胁迫。花生籽仁的氮素积累量占全株氮素积累量的68.42%~77.67%。与WWMN处理相比,FDMN处理下花生各器官氮肥吸收比例(Ndff,the percentage of N derived from ^(15)N fertilizer)和^(15)N积累量显著提高,且促进了氮素向籽仁的转运,PDMN处理下籽仁^(15)N积累量却显著降低。花生对^(15)N标记氮肥的回收率为30.20%~38.42%,残留率为37.12%~48.83%,损失率为12.75%~32.68%。FDMN处理下氮肥回收率最高,损失率最低。表明90 kg hm^(-2)的施氮水平促进了干旱胁迫下花生产量的提高和氮素吸收利用,降低了肥料氮的损失。展开更多
目的:观察大鼠酒精性肝病组织病理形态学改变,探讨细胞凋亡与细胞色素P4502E1的表达以及和氧化应激的关系.方法:用酒精灌胃法制备酒精性肝病大鼠模型,模型组给予酒精8 g/kg,每天分2次灌胃连续8 wk,对照组给予等量的生理盐水灌胃.实验8 w...目的:观察大鼠酒精性肝病组织病理形态学改变,探讨细胞凋亡与细胞色素P4502E1的表达以及和氧化应激的关系.方法:用酒精灌胃法制备酒精性肝病大鼠模型,模型组给予酒精8 g/kg,每天分2次灌胃连续8 wk,对照组给予等量的生理盐水灌胃.实验8 wk末,观察肝组织的病理形态学改变,用原位末端标记法(TUNEL)检测肝细胞凋亡,用免疫组化法检测肝组织中Caspase-3蛋白表达,用全自动生化仪检测ALT和AST的含量,用PCR法测定肝细胞色素P4502E1的基因表达,分别用硫代巴比妥酸法(TBA法)和黄嘌呤氧化酶法测定肝组织丙二醛(MDA)的含量和超氧化物歧化酶(SOD)的活力.结果:模型组凋亡的肝细胞明显增多,主要分布在中央静脉周围、点状和灶状坏死区;Caspase-3主要分布于中央静脉及肝细胞坏死灶周围细胞的胞质中.模型组肝细胞凋亡指数(AI)和Caspase-3蛋白表达强度明显高于对照组(AI:6.2%±1.7% vs 1.7%±0.8%;Caspase-3:66.7% vs 9.5%,P<0.05,P<0.01).CYP2E1表达:对照组c1基因频率为91.6%,c2基因频率为8.4%;模型组c1基因频率为53.4%,c2基因频率为46.6%,均有显著性差异(P<0.05).长期酒精摄入大鼠血清MDA含量增加(41.53±7.43μmol/L vs 15.72±2.06μmol/L,P<0.05),SOD活力下降(353.12±61.02 kU/L vs 636.82±138.60 kU/L,P<0.05),与酒精性肝病肝细胞凋亡程度有相关性(r=0.644,r=-0.511).结论:长期酒精摄入可引起大鼠酒精性肝病及及肝功能损伤,肝细胞凋亡明显增加.CYP2E1基因PstⅠ及RsaⅠRFLPs与酒精性肝病有关,其中c2基因可能与大鼠酒精性肝病的发生有关.MDA含量和SOD活力在酒精性肝病的肝细胞凋亡过程及脂质过氧化反应中发挥重要作用.展开更多
基金Supported by the Basic Research Foundation of Beijing Institute of Technology(20141642002)
文摘Detecting protein expression levels in organisms exposed to environmental pollutants can help us understand the action mechanism of toxicity. In the present study, adult female Sprague-Dawley (SD) rats received a consecutive intraperitoneal injection of tris(2,3-dibromopropyl) isocyanurate (TBC) for seven days, and the ^16O/^18O labeling comparative proteomic approach was used to study the change of liver proteome in these rats. Thirty six differential proteins were identified between the control group and the high-dose-exposed group (8 mg/kg), of which, twenty eight proteins were down-regulated and eight proteins were up-regulated. Bioinformatics analysis revealed that most of the differential proteins were related with the metabolic and cellular processes. In addition, the contents of malondialdehyde (MDA), catalase (CAT), and glutathione (GSH) in liver were measured to evaluate the oxidative stress status induced by TBC exposure. Together, these findings showed that TBC might be toxic to liver by disrupting metabolic process and apoptosis. The results might provide a better insight into the mechanism of toxicity induced by TBC.
文摘为明确不同生育期干旱胁迫与氮肥施用对花生氮素吸收利用的影响,利用^(15)N示踪技术,研究了不同水分条件下氮肥施用对花生各器官肥料氮吸收利用以及氮肥残留和损失情况的影响。水分设置为正常供水(WW,75%~80%田间持水量)、花针期轻度干旱胁迫(FD,55%~60%田间持水量)和结荚期轻度干旱胁迫(PD,55%~60%田间持水量)3个条件,氮肥水平设置为不施氮(LN)、中氮(MN,90 kg hm^(-2))、高氮(HN,180 kg hm^(-2))。结果表明,与正常供水条件相比,不同生育期干旱胁迫均降低了花生产量和植株氮素积累量,且花针期干旱胁迫的降低幅度大于结荚期干旱胁迫。花生籽仁的氮素积累量占全株氮素积累量的68.42%~77.67%。与WWMN处理相比,FDMN处理下花生各器官氮肥吸收比例(Ndff,the percentage of N derived from ^(15)N fertilizer)和^(15)N积累量显著提高,且促进了氮素向籽仁的转运,PDMN处理下籽仁^(15)N积累量却显著降低。花生对^(15)N标记氮肥的回收率为30.20%~38.42%,残留率为37.12%~48.83%,损失率为12.75%~32.68%。FDMN处理下氮肥回收率最高,损失率最低。表明90 kg hm^(-2)的施氮水平促进了干旱胁迫下花生产量的提高和氮素吸收利用,降低了肥料氮的损失。
文摘目的:观察大鼠酒精性肝病组织病理形态学改变,探讨细胞凋亡与细胞色素P4502E1的表达以及和氧化应激的关系.方法:用酒精灌胃法制备酒精性肝病大鼠模型,模型组给予酒精8 g/kg,每天分2次灌胃连续8 wk,对照组给予等量的生理盐水灌胃.实验8 wk末,观察肝组织的病理形态学改变,用原位末端标记法(TUNEL)检测肝细胞凋亡,用免疫组化法检测肝组织中Caspase-3蛋白表达,用全自动生化仪检测ALT和AST的含量,用PCR法测定肝细胞色素P4502E1的基因表达,分别用硫代巴比妥酸法(TBA法)和黄嘌呤氧化酶法测定肝组织丙二醛(MDA)的含量和超氧化物歧化酶(SOD)的活力.结果:模型组凋亡的肝细胞明显增多,主要分布在中央静脉周围、点状和灶状坏死区;Caspase-3主要分布于中央静脉及肝细胞坏死灶周围细胞的胞质中.模型组肝细胞凋亡指数(AI)和Caspase-3蛋白表达强度明显高于对照组(AI:6.2%±1.7% vs 1.7%±0.8%;Caspase-3:66.7% vs 9.5%,P<0.05,P<0.01).CYP2E1表达:对照组c1基因频率为91.6%,c2基因频率为8.4%;模型组c1基因频率为53.4%,c2基因频率为46.6%,均有显著性差异(P<0.05).长期酒精摄入大鼠血清MDA含量增加(41.53±7.43μmol/L vs 15.72±2.06μmol/L,P<0.05),SOD活力下降(353.12±61.02 kU/L vs 636.82±138.60 kU/L,P<0.05),与酒精性肝病肝细胞凋亡程度有相关性(r=0.644,r=-0.511).结论:长期酒精摄入可引起大鼠酒精性肝病及及肝功能损伤,肝细胞凋亡明显增加.CYP2E1基因PstⅠ及RsaⅠRFLPs与酒精性肝病有关,其中c2基因可能与大鼠酒精性肝病的发生有关.MDA含量和SOD活力在酒精性肝病的肝细胞凋亡过程及脂质过氧化反应中发挥重要作用.
